1) The most suitable carbon source for 5-ketofructose fermentation by Gluconobacter suboxydans Strain 1 was confirmed to be D-sorbitol or L-sorbose using growing and resting cells. D-Fructose had little effect on the formation of this dicarbonylhexose.
2) The optimal pH for the formation from L-sorbose by intact cells was found to be at 4.2.
3) The activity of the pentose phosphate cycle in the resting cells was calculated as 13_??_17μatoms/hr/mg of dry cells by the use of the manometric techniques.
4) There was no strain tested so far which could accumulate a large amount of 5-keto-D-fructose from D-sorbitol except this bacterium.
5) The experimental results shown in this paper makes the prediction that a certain dehydrogenating system of L-sorbose is functional in the organism, and the metabolic pathways of D-sorbitol via L-sorbose and 5-keto-D-fructose is proposed.

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