Diploid potato breeding populations with quantitatively inherited traits related to pest resistance were selected. These lines were further crossed to 4x cultivars via FDR 2n pollen to examine the level of transmission of variable traits including the resistance to bacterial wilt (BW), potato tuber moth (PTM), and root-knot nematodes (RKN). Transmission of quantitative traits including late blight (LB) resistance and the presence of glandular trichomes which confer general insect resistance in tetraploid genotypes was also examined in the 4x × 2x progeny. All the quantitative traits except for the resistance to PTM were transmitted to progeny with a high frequency of resistant progenies. Among 557 clones from 4x × 2x crosses, 114 clones showed a combination of two types of quantitative resistance traits in a single clone. Four types of cornbinations, BW + RKN (85 clones), LB + BW (14 clones), BW + glandular trichonres (1 clone), and RKN + glandular trichomes (14 clones), were identified. Our results indicated that: 1) the transmission of combined quantitative traits related to pest resistance was possible via FDR 2n pollen; and 2) quantitative traits related to LB resistance and the presence of glandular trichomes could be transmitted to progeny with a high frequency.