The genome sequence of the Common Emerald, Hemithea aestivaria (Hübner, 1789)

We present a genome assembly from an individual male Hemithea aestivaria (the Common Emerald; Arthropoda; Insecta; Lepidoptera; Geometridae). The genome sequence is 501.7 megabases in span. Most of the assembly is scaffolded into 31 chromosomal pseudomolecules, including the Z sex chromosome. The mitochondrial genome has also been assembled and is 17.05 kilobases in length. Gene annotation of this assembly on Ensembl identified 18,477 protein coding genes.


Background
The 'emeralds' are a group of over 2000 moth species, most of which have characteristic blue-green wings.Phylogenetic analysis using a small number of genes suggests the group is monophyletic and it is currently classified as a distinct subfamily Geometrinae within the family Geometridae (Ban et al., 2018;Sihvonen et al., 2011).Of the 10-12 species found in Britain and Ireland, the Common Emerald, Hemithea aestivaria, is one of the most widespread and can be recognised by its dark green angular wings with black and white chequered fringes.
The geographic range of H. aestivaria spans much of Eurasia, from Portugal and Ireland to Japan and Korea (GBIF Secretariat, 2022).In Britain, the moth is most common in the southern counties of England and has a northern limit in the south of Scotland (Randle et al., 2019).In Europe, the moth in univoltine with the adult flying in summer; the polyphagous larvae feed on low-growing herbaceous plants in autumn, and after overwintering eat the leaves of woody trees and bushes (South, 1961).H. aestivaria is recorded as bivoltine in Japan (Hausmann, 2001).The species is thought to have been introduced accidentally to North America: larvae were first recorded on fruit trees in British Columbia in 1973 before the species spread south to Oregon and Washington State where it is a minor pest of apple orchards (Doǧanlar & Beirne, 1979;LaGasa, 1996;Looney et al., 2016;Schmidt & Anctil, 2021).Since 2019, H. aestivaria has also been recorded on the east coast of Canada in Ontario, Québec and Nova Scotia (Schmidt & Anctil, 2021).
The green colour of emerald moths has long intrigued entomologists due to its propensity to fade in living individuals and in dried museum specimens.It also a recognisably different shade to the green on other lepidopteran wings.The colour is conferred by a pigment located in granules within the wing scales; extractions using wings of H. aestivaria and other emerald moths has shown it to be a light-sensitive polar molecule, most likely bound to protein (Cook, 1993;Cook et al., 1994).The chemical structure of the pigment (named geoverdin) and the biochemical pathway for its production have not been determined.
The genome sequence of Hemithea aestivaria was determined as part of the Darwin Tree of Life project.The assembled genome sequence will facilitate research into the biochemistry underpinning pigment synthesis in insects, and contribute to the growing set of resources for studying lepidopteran ecology and evolution.

Genome sequence report
The genome was sequenced from one male Hemithea aestivaria (Figure 1) collected from Wytham Woods,Oxfordshire (51.77,.A total of 36-fold coverage in Pacific Biosciences single-molecule HiFi long reads was generated.Primary assembly contigs were scaffolded with chromosome conformation Hi-C data.Manual assembly curation corrected 67 missing joins or mis-joins and removed 17 haplotypic duplications, reducing the assembly length by 0.64% and the scaffold number by 10.48%, and increasing the scaffold N50 by 0.48%. The final assembly has a total length of 501.7 Mb in 93 sequence scaffolds with a scaffold N50 of 17.6 Mb (Table 1).Most (99.38%) of the assembly sequence was assigned to 31 chromosomal-level scaffolds, representing 30 autosomes and the Z sex chromosome.Chromosome-scale scaffolds confirmed by the Hi-C data are named in order of size (Figure 2-Figure 5; Table 2).While not fully phased, the assembly deposited is of one haplotype.Contigs corresponding to the second haplotype have also been deposited.The mitochondrial genome was also assembled and can be found as a contig within the multifasta file of the genome submission.
Metadata for specimens, spectral estimates, sequencing runs, contaminants and pre-curation assembly statistics can be found at https://links.tol.sanger.ac.uk/species/572857.

Sample acquisition and nucleic acid extraction
The specimen selected for genome sequencing was a male Hemithea aestivaria (ilHemAest2), collected from Wytham Woods, Oxfordshire (biological vice-county Berkshire), UK (latitude 51.77, longitude -1.34) on 2020-07-05.The specimen was taken from woodland habitat by Douglas Boyes (University of Oxford) using a light trap.The specimen was identified by the collector, and then snap-frozen on dry ice.
DNA was extracted at the Tree of Life laboratory, Wellcome Sanger Institute (WSI).The ilHemAest2 sample was weighed and dissected on dry ice with tissue set aside for Hi-C sequencing.Whole organism tissue was disrupted using a Nippi Powermasher fitted with a BioMasher pestle.High molecular weight (HMW) DNA was extracted using the Qiagen MagAttract HMW DNA extraction kit.HMW DNA was sheared into an average fragment size of 12-20 kb in a Megaruptor 3 system with speed setting 30.Sheared DNA was purified by solid-phase reversible immobilisation using AMPure PB beads with a 1.8X ratio of beads to sample to remove the shorter fragments and concentrate the DNA sample.The concentration of the sheared and purified DNA was assessed using a Nanodrop spectrophotometer and Qubit Fluorometer and Qubit dsDNA High Sensitivity Assay kit.Fragment size distribution was evaluated by running the sample on the FemtoPulse system.

Sequencing
Pacific Biosciences HiFi circular consensus d DNA sequencing libraries were constructed according to the manufacturers' instructions.Poly(A) RNA-Seq libraries were constructed using the NEB Ultra II RNA Library Prep kit.DNA and RNA sequencing was performed by the Scientific Operations core at the WSI on the Pacific Biosciences SEQUEL II (HiFi) instrument.Hi-C data were also generated from tissue of ilHemAest2 that had been set aside, using the Arima2 kit and sequenced on the Illumina NovaSeq 6000 instrument.A Hi-C map for the final assembly was produced using bwa-mem2 (Vasimuddin et al., 2019) in the Cooler file format (Abdennur & Mirny, 2020).To assess the assembly metrics, the k-mer completeness and QV consensus quality values were calculated in Merqury (Rhie et al., 2020).This work was done using Nextflow (Di Tommaso et al., 2017) DSL2 pipelines "sanger-tol/readmapping" (Surana et al., 2023a) and Further, the Wellcome Sanger Institute employs a process whereby due diligence is carried out proportionate to the nature of the materials themselves, and the circumstances under which they have been/are to be collected and provided for use.

Genome assembly, curation and evaluation
The purpose of this is to address and mitigate any potential legal and/or ethical implications of receipt and use of the materials as part of the research project, and to ensure that in "sanger-tol/genomenote" (Surana et al., 2023b).The genome was analysed within the BlobToolKit environment (Challis et al., 2020) and BUSCO scores (Manni et al., 2021;Simão et al., 2015) were calculated.
Table 3 contains a list of relevant software tool versions and sources.

Genome annotation
The BRAKER2 pipeline (Brůna et al., 2021) was used in the default protein mode to generate annotation for the Hemithea aestivaria assembly (GCA_947507615.1) in Ensembl Rapid Release.

Wellcome Sanger Institute -Legal and Governance
The materials that have contributed to this genome note have been supplied by a Darwin Tree of Life Partner.The submission

Arun Arumugaperumal
Department of Biotechnology, Rajalakshmi Engineering College, Chennai, Tamilnadu, India This is the first report of the genome sequence of the common emerald Hemithea aestivaria.The genome is of size 500 Mbp and has 31 chromosomes.The authors have predicted 18,477 genes in the genome.A 17 kbp mitochondrial genome was also assembled.The genome sequence was shown to be 98% complete through BUSCO analysis.The genome can be accessed from NCBI using the identifier GCA_947507615.1.
The photograph of the specimen could have been taken with a standard background along with a scale.The authors could have predicted the non-coding RNA genes and provided the data also.

Is the rationale for creating the dataset(s) clearly described? Yes
Are the protocols appropriate and is the work technically sound?Yes

Are sufficient details of methods and materials provided to allow replication by others? Yes
Are the datasets clearly presented in a useable and accessible format?Yes Competing Interests: No competing interests were disclosed.

Figure 2 .
Figure 2. Genome assembly of Hemithea aestivaria, ilHemAest2.1:metrics.The BlobToolKit Snailplot shows N50 metrics and BUSCO gene completeness.The main plot is divided into 1,000 size-ordered bins around the circumference with each bin representing 0.1% of the 501,713,186 bp assembly.The distribution of scaffold lengths is shown in dark grey with the plot radius scaled to the longest scaffold present in the assembly (23,607,823 bp, shown in red).Orange and pale-orange arcs show the N50 and N90 scaffold lengths (17,632,203 and 11,810,528 bp), respectively.The pale grey spiral shows the cumulative scaffold count on a log scale with white scale lines showing successive orders of magnitude.The blue and pale-blue area around the outside of the plot shows the distribution of GC, AT and N percentages in the same bins as the inner plot.A summary of complete, fragmented, duplicated and missing BUSCO genes in the lepidoptera_odb10 set is shown in the top right.An interactive version of this figure is available at https://blobtoolkit.genomehubs.org/view/ilHemAest2.1/dataset/CANNRZ01/snail.

Figure 5 .
Figure 5. Genome assembly of Hemithea aestivaria, ilHemAest2.1:Hi-C contact map of the ilHemAest2.1 assembly, visualised using HiGlass.Chromosomes are shown in order of size from left to right and top to bottom.An interactive version of this figure may be viewed at https://genome-note-higlass.tol.sanger.ac.uk/l/?d=b1h_oRM_RIu7RhwFE-mCfA.