Serum Levels of MDC and MMP-9 and the Relationship Between Serum Levels and Disease Activity in the Patients with Systemic Lupus Erythematosus

Background and Objective: Systemic lupus erythematosus (SLE) is a complicated autoimmune disease. Although its pathogenesis is not clear, cytokine may be involved in it. So we investigated serum levels of macrophage-derived chemokine (MDC), and matrix metalloproteinase-9 (MMP-9), and to determine the relationship between serum levels and the disease activity of SLE. Methods: Serum levels of MDC and MMP-9 were measured by enzyme-linked immuno sorbent assay (ELISA). Results: Significantly decreased serum levels of MDC and MMP-9 were found in SLE as compared to those in controls (P<0.001 P<0.001), but serum levels of MDC and MMP-9 increased after treatment (P<0.001 P<0.05). Serum levels of MDC and MMP-9 were lower in patients with active disease than those with inactive disease (P<0.001 P<0.05). Significantly decreased serum levels of MDC and MMP-9 were found in patients with renal damage than those without the damage (P<0.001 P<0.05). Serum level of MDC was lower in patients with arthritis than those without the damage (P<0.001), but serum level of MMP-9 has no significant difference in two groups (P>0.05). Conclusion: The present data suggest that MDC and MMP-9 may be involved in the pathogenesis of SLE, and serum levels of MDC and MMP-9 could be markers of monitoring disease activity, renal damage, disease progression and improvement in SLE.

evaluated by the SLEDAI (SLE Disease Activity Index). 2 The patients with SLE (32 women and 4 men, aged 11-61 yrs) were divided into 2 groups: 13 SLE patients with renal disease and 23 SLE patients without renal disease according to proteinuria (++↑ or >0.5g/d); 23 active course patients and 13 inactive course patients were evaluated using the SLEDAI standard, and active disease was indicated by SLEDAI score of more than 8 points; 23 SLE patients with arthritis and 13 without the lesion according to articular damage. 30 sex and age matched healthy volunteers were recruited as controls. Serum: Three milliliters of venous peripheral blood were collected from each subject, centrifuged to get serum, and then stored at -80ºC. Nine serum samples were collected from patients taking corticosteroid three or four weeks later. The concentrations of MDC and MMP-9 were measured by ELISA respectively. ELISA kits for HGF, MDC and MMP-9 were purchased from R&D Systems Inc (Catalog No: DMD00, DMP00). Assays were performed according to the manufacturer's instructions. Statistical analysis: All the results were presented as mean±standard deviatioin. The differences were tested for statistical significance by Student's t test and relative risk. A probability (P) less than 0.05 was considered as significantly different.

DISCUSSION
SLE is a complicated autoimmune disease characterized by various immunological abnormalities, including polyclonal activation of circulating B lymphocyte that produce a large quantity of autoreactive antibodies, the abnormality of T lymphocyte and IC deposition. During the process, many chemokines and their receptors play an important regulating role. 3 If monocyte/macrophage releases IL-10 continuously, it will result in division of Th cell to Th2 cell. If monocyte/macrophage releases IL-12 continuously, it will promote transformation of Th cell to Th1 cell. So we focus on cytokines derived from monocyte/macrophage and this will demonstrate the role of chemokines in SLE pathogenesis.
MDC, a CC chemokine, is a potent chemoattractant which activated Th2 lymphocytes via the chemokine receptor CCR4, 4 and its receptor CC chemokine receptor 4 (CCR4) preferentially MMP-9 was involved in inflammation and immune system dysfunctions. Besides immunologic abnormalities, SLE also presents chronic inflammatory components. Therefore, a role of MMP-9 in SLE pathology might be suspected. 8 Serum level of MMP-9 in 36 SLE patients was tested and it was discovered that serum level of MMP-9 was significantly decreased in patients as compared to those in controls, and was lower in patients with active disease than those with inactive disease. A negative relation between serum level of MMP-9 and SLEDAI was found, which may reflect dynamic change of SLE. SLE tends to damage multiple organs, including the kidney and article. Our test showed the serum levels of MDC and MMP-9 were decreased in patients with renal damage than those without the damage, and the serum level of MDC was markedly decreased in patients with arthritis than those without the damage. So MDC and MMP-9 may be involved in the pathogenesis of lupus nephritis and seemed to be markers of renal damage, and MDC may be involved in the pathogenesis of arthritis and seemed to be a new sensitive marker of article damage. As Garcia 9 and his colleagues reported, they found that MDC was critically involved in the development at anti-GBM GN from acute glomerular injury to irreversible tissue damage. Sato 10 and his colleagues investigated the serial changes of glomerular metalloproteinase activity in antithymocyte-induced glomerulonephritis in rats and found attennated glomerular MMP-9 activity.
The serum levels of MDC and MMP-9 in 9 SLE patients taking corticosteroid 3 or 4 weeks later were examined and it was found that clinical symptoms were relieved, indexes of lab test improved, serum level of MDC and MMP-9 increased. The increased serum level of MDC remains to be studied, but the increased serum level of MMP-9 may explain that glucocortisone influenced the release of MMP-9 from blood circulation. 11 It is thought that the increased serum level of MDC and MMP-9 may demonstrate the lupus improvement, so the serum levels of MDC and MMP-9 may serve as markers for the determination of disease progression or improvement.
MDC and MMP-9 were involved in the pathogenesis of SLE. What is their relationship remains to be studied. Vermaelen 12 and his colleagues reported that the specific absence of MMP-9 activity inhibited the development of allergic Serum levels and disease activity airway inflammation by impairing the recruitment of DCs into airways and the local production of DCderived chemokines. At present, DCs are the most powerful antigen presenting cells and the main resource of MDC. 13 The functional disorder antigen presenting cells 14 and the reduced DCs 15 are in existence in patients with SLE has been reported and the proportion of DCs in peripheral blood showed a negative correlation with disease activity. 15 From our study it has been shown that serum levels of MDC and MMP-9 were significantly decreased in SLE patients. Whether this mechanism may also be involved in SLE pathogenesis, it depends on further accumulation of clinical researches.

CONCLUSION
SLE is an autoimmune disease. The useful measurement to cure this disease is Prednisone. When symptom ease, the dosage of Prednisone should be reduced. At this time, patient's condition prefers to relapse, especially during 10-15mg a day to take. So the combing between Traditional and Western medicine is very important. The combination can control its deterioration and reduce its relapse so it is better to cure it.

Limitations of the Study:
Small sample size was one of the important limitations of this study.