Detection of Vancomycin Resistant Enterococci from Rectal Swab Samples by Becton-Dickinson GeneOhm VanR assay and Culture at ICU of a Tertiary Care Center in Turkey.

Objectives: Vancomycin resistance is due to change in ligase enzyme that destroys the binding of the drug. The gold standard is culture; but now molecular methods have also been developed. The aim was to detect the VRE rate at ICUs by culture and BD GeneOhm™ VanR and compare the results of both assays. Methodology: 135 perianal swabs were taken from the patients at ICUs between January 1st 2009 and April 30th 2009. Samples were identified by conventional methods and BD GeneOhm VanR assay. Results: In newborn ICU, 41 patients (74.6%) were negative by both methods. Two (3.6%) were positive by both methods. Twelve (21.8%) of them were culture negative and PCR positive. In adult ICU, 73 (91.3%) patients were negative by both methods. Seven patients (8.8%) were positive by molecular method only. Conclusion: This study showed low VRE positivity due to factors like inhibition in PCR or culture negativity due low inoculum for bacterial growth. Early detection of VRE is an important issue especially in ICUs and molecular techniques are important tools; but against all, we still need to confirm this method with culture based techniques and in order to do this further studies with higher number of patients with VRE colonisation are required.


INTRODUCTION
Enterococci which are Gram positive cocci in chain forming can lead to nosocomial and community-acquired infections. 1 They are a part of colon flora and can grow in 6.5% NaCl containing blood agar medium. 2 Vancomycin is an important treatment choice for enterococci. 3 Vancomycinresistant enterococcus (VRE) became one of the most important causes of nosocomial infection after being first identified in 1986. 4 The most common method to detect VRE isolates is the culture based methods that take 1-5 days. [5][6][7] from perianal swabs. This rapid molecular method helps to detect VRE carriers in less amount of time compared to culture based methods which may take days to report the result and also provide the chance of avoiding the spread from one patient to another. The aim of our study was to detect the rate of vancomycin resistant enterococci isolated from perianal swab samples of the patients hospitalized at intensive care units of Tepecik Education and Research Hospital by culture and Becton-Dickinson GeneOhm™ VanR assay and compare the results of both assays.

METHODOLOGY
Perianal swab samples were taken from the patients hospitalized at the intensive care units of Tepecik Education and Research Hospital between January 1 st 2009 and April 30 th 2009. Patients' demographic data and underlying diseases were also recorded. Both samples for RT-PCR and culture methods were from the same (duplicated) perianal swabs. The identification was done by BBL CRYSTAL Gram-Positive (GP) identification (ID) system (Becton-Dickinson, USA). The antimicrobial susceptibility was studied by Kirby-Bauer disk diffusion method. Penicilin (10U), ampicilin/sulbactam (10μg/10μg), gentamycin (120μg), streptomycin (300μg), ciprofloxacin (5μg), levofloksasin (5μg), erythromycin (15μg), linezolid (30μg), vancomycin (30μg) and teikoplanin (30μg) antimicrobial disks (Becton-Dickinson, USA) were applied. 8 The MIC values of vancomycin resistant isolates were studied by E-test (AB, Biodisk, Solna, Sweeden). The BD GeneOhm™ VanR Assay is a qualitative in vitro test for the rapid detection of vancomycin-resistance (vanA and vanB) genes directly from perianal swabs. The assay was performed and evaluated according to manufacturer's instructions. 9

RESULTS
A total of 135 patients were included into the study, 71(52.6%) were male, 64 (47.4%) were female. There were 55 newborns and 80 adult patients. The demographic data and the underlying disorders are shown in Table- The antimicrobial suceptibility of E. faecalis isolates were 5.2% to penicillin, 5.2% to ampicilinsulbactam, 13.1% to erithromycin, 28.9% to cipro-   Table-IV. of 6 µg/ml vancomycin containing agar. 8 The first VRE isolate in Turkey was detected in 1998 at Akdeniz University Hospital and the reports followed there after. 13 Tufan ZK et al reported a study on screening of VRE carriers in total of 508 intensivecare unit patients and health care staff. Risk factors such as previous antibiotic use, especially vancomycin and cephalosporin, the presence of invasive devices like catheters, and co-morbid diseases were investigated. Rectal smear cultures were obtained from each participant to detect VRE colonization. Except for one patient, who had been transferred from another hospital, no VRE colonization was detected in patients or health care staff. 14 Yis R et al evaluated the status of VRE colonization in Oncology Department of Gaziantep Children's Hospital following a VRE isolation from the urine sample of a patient. 15 Perirectal swab samples were collected from patients. A total of 123 perirectal swab specimens obtained from patients staying at oncology, burn, pediatric surgery and ICUs were investigated and the rate of VRE colonization was 14.6%. In our study the rate of VRE positivity was less than this one; because of the fact that our study was not conducted during an outbreak as stated above and in the study above they did not apply a molecular method as well which could have detected the VRE positivity earlier. 15 Zer Y et al investigated the molecular epidemiology and antibacterial resistance of Enterococci strains isolated from in-patients of the 800-bed capacity research hospital of the Faculty of Medicine, University of Gaziantep between January 2009 and August 2010. vanA gene-type resistance was recorded in 76 (93.8%) of the strains, vanB genetype resistance in two (2.5%) and nonA-nonB type resistance gene in three (3.7%) of the strains. 16 In our study we had only two positive patients by both Ayhan Gozaydin et al.

DISCUSSION
The first isolates of VRE were reported at the end of 1980s. After this VRE infection and colonisation showed an increase. 10 The resistance is troublesome for the management of hospitalized patients. Preventing the spread of VRE is an important matter. 11 The detection of VRE is based on culture, which requires 24 to 72 hours. 12 CLSI recommends the use methods and had less van A and van B positivity and this may be due to inhibition in molecular method or strict application of infection control measures. Most of the studies that we considered in Turkey are about the evaluation of outbreaks and clonal relationship; but in our study we evaluated the VRE rate at ICUs not during an outbreak status. Effective infection control measures may be a factor for not having a high VRE prevalence. Many investigators worked on the detection of vanA or vanB from rectal samples or colonies by PCR. These methods have the advantage of giving rapid results compared to culture based methods. Isolation from samples can have the problem of inhibition. [17][18][19] Werner G et al compared direct cultivation on a selective solid medium, polymerase chain reaction from an enrichment broth, and the BD GeneOhm™ VanR Assay for identification of vancomycin-resistant enterococci in screening specimens. They reported the performance of the assay and compared with culture-based methods and subsequent PCR analysis in two university hospitals with a different VRE prevalence. A total of 1786 samples were analyzed. The overall sensitivity was 93.1%; the specificity was 87.0% mainly due to false-positive vanB results. 20 Usacheva EA et al reported the multicenter evaluation of the BD GeneOhm VanR assay for direct, rapid detection of vancomycin-resistant Enterococcus species in perianal and rectal specimens. They studied 1,027 perianal and rectal swab specimens from three geographically distinct US sites. Direct swab specimens were tested by the assay and compared with direct culture. The sensitivity, specificity, and positive and negative predictive values of the assay were 93.2%, 81.9%, 54.4%, and 98.1%. According to the authors the specificity was limited because of false-positive results in the vanB portion of the assay. Specificity with perianal swabs was significantly greater than with rectal swabs. They think that the assay is a simple, rapid, and acceptable method for screening for VRE in a variety of populations in which vanA is the predominant genotype. Samples positive for the vanB genotype should be confirmed by culture owing to the apparent high number of false-positive results. 21 In our study 43 Enterococcus spp. isolates were obtained from 135 perianal swab samples; but only two (3.6%) samples taken from the newborn ICU, had vancomycin resistance (vanA type) that was detected both by the BD GeneOhm™ VanR assay and culture. BD GeneOhm VanR assay detected vanA type resistance in sixteen samples (11.8%), vanB type resistance in four samples (2.9%) and one sample (0.7%) had both vanA and vanB types of vancomycin resistance. Only two E. faecium isolates isolated at newborn ICU were resistant to vancomycin and teicoplanin. The most common underlying diseases at adult ICU were pneumoniae (16.2%), cerebrovascular diseases (13.7%) and 31.2% of them had multiple underlying diseases. In adult ICU most of the patients had multiple underlying diseases and that may be an additional factor although none of the patients were confirmed by culture based techniques. The newborns had prematurity (70.9%), respiratuary distress syndrome (49.1%), transient tachypneae (7.5%). These underlying disorders in newborn ICU could have attributed to colonisation of VRE. Also two VRE positive babies could be affected by VRE carrier state of their mothers and besides they could have been transferred from another center already being a VRE carrier. Risk factors such as renal failure, GIS operation, diabetes mellitus, malignity and corticosteroid treatment could also have contributed to VRE colonisation detected by only molecular method in both of the ICUs.
The sensitivity of the assay was 100% due to lack of false negative results; and the specifity was 85.7%. The positive predictive value (PPV) was 9.5% and the negative predictive value (NPV) was 100%. The PPV mainly depends on the prevalance of that population and that may be the cause of low PPV. The low prevalance in our study group may be due to effective infection control measures; although the population that we have screened had underlying disorders, treated with broad spectrum antibiotics and hospitalized at ICUs. We also had discordant results such as simultaneous PCR positivity and culture negativity that can be due to dead enterococci. We had more PCR positivity than culture positivity. This can be due to poor culture growth, inhibition, contamination or not having enough inoculum for bacterial growth. Although molecular methods have the advantage of rapidity and sensitivity; they also have some disadvantages like high cost, DNA extraction problems, detecting DNA although the bacteria being dead, contamination, inhibition and false positivity. 7,22,23 As a conclusion culture based methods require at least 48-72 hours; but PCR assays result is available in two to three hours. The most important disadvantage of these molecular assays is inhibition due to feacal samples and contamination. Against all, molecular methods decreased hospital stay and cost. GeneOhm VanR assays can be used as VRE screening tests in populations that show a predominant BD GeneOhm, VanR assay and culture based methods colonization with vanA containing enterococci, while vanB positive samples need to be confirmed by another method for the existence of VRE. In our study we detected more VRE colonisation by PCR compared to culture which may be due to detecting bacteria already being dead in PCR, poor culture growth or not having enough for bacterial growth. Early detection of VRE is an important issue especially in ICUs and molecular techniques are important tools; but we still need to confirm this method with culture based techniques and in order to do this further studies with higher number of patients with VRE colonisation are required.