A reciprocal cross difference in the frequency of male recombination was detected in progeny of hybrids between e se; bri ru and Tonga stocks of Drosophila ananassae. A chromosomal enhancer-cytoplasmic suppressor system determining male crossing over in D. ananassae was identified that appeared to be similar to, but distinct from, that described previously by Hinton (1974). The higher the temperature at which males were reared, the less reciprocal cross ratio in the frequency of male recombination was found. The reciprocal cross differences might be explained by a cytoplasmic suppressor of the Tonga stock and a nuclear chromosomal gene(s). This chromosomal gene is dominant for the reducing the frequency of male recombination, and is located on the 2nd chromosome of the Tonga stock. The maternally transmitted cytoplasmic suppressor was maintained by other chromosomal regions (genes) on both 2nd and 3rd chromosomes of the Tonga stock.