Osteoarthritis (OA) is characterized by inflammation of the joints and degradation of articular cartilage matrix, but treatment remains symptomatic and effective therapies have yet to be established. In OA, various inflammatory stimuli are applied to chondrocytes, resulting in cellular damage. Therefore, elucidating the regulatory mechanisms mediating inflammatory responses in chondrocytes is crucial. REV-ERBs function as transcriptional repressors in inflammatory responses, but the roles of REV-ERBs in OA have not been clarified. Then, the current study investigated the effect of REV-ERB agonist on the expression of inflammatory cytokines and proteolytic enzymes in chondrocyte stimulated with lipopolysaccharide (LPS). The chondrocytes were prepared from the knee articular cartilage of neonatal Wistar rats. Messenger RNA expression was measured by real-time PCR. Treatment of chondrocytes with LPS increased the mRNA expression of degradation enzymes of extracellular matrix (matrix metalloproteinase (MMP)-3, MMP-13) and proinflammatory cytokines (interleukin-1β, tumor necrosis factor-α). However, pretreatment with SR9009 significantly blocked these responses. These results suggest that REV-ERBs might be involved in the regulation of inflammatory responses and degradation of extracellular matrix in chondrocytes.