Monitoring Expression of Cytochrome P450 Genes During Postischemic Rat Liver Reperfusion Using DNA Microarrays

Yasuo Takahashi; Yayoi Nishida; Yukimoto Ishii; Koichi Ishikawa; Satoshi Asai

doi:10.1254/jphs.RC0040040

Rapid Communication

Monitoring Expression of Cytochrome P450 Genes During Postischemic Rat Liver Reperfusion Using DNA Microarrays

Yasuo Takahashi, Yayoi Nishida, Yukimoto Ishii, Koichi Ishikawa, Satoshi Asai

Author information

Keywords: cytochrome P450, postischemic liver reperfusion, gene expression

JOURNAL FREE ACCESS

2005 Volume 97 Issue 1 Pages 153-156

DOI https://doi.org/10.1254/jphs.RC0040040

View "Advance Publication" version (January 15, 2005).

Details

Abstract

We investigated the expression of 50 cytochrome P450 (CYP) genes in the postischemic reperfused rat liver using a DNA microarray. Thirteen CYPs showed absent expression in all experiments and 2 CYPs were induced by pentobarbital, which was used as an anesthetic. Eight of the remaining 35 CYPs showed significantly decreased expression following ischemia-reperfusion. Monitoring CYP expression may be a powerful approach for elucidation of pathways regulating drug metabolism that may be involved in postischemic reperfusion liver injury.

Corresponding author

Register with J-STAGE for free!