NG-Nitro-<sc><font size = "-2">L</font></sc>-arginine Methyl Ester-Induced Potentiaton of the Effect of Aminophylline on Rat Diaphragm: the Role of Extracellular Calcium

Radan Stojanovic; Zoran Todorovic; Zorica Nesic; Sonja Vuckovic; Natasa Cerovac-Cosic; Milica Prostran

doi:10.1254/jphs.SCE04001X

Short Communication

N^G-Nitro-L-arginine Methyl Ester-Induced Potentiaton of the Effect of Aminophylline on Rat Diaphragm: the Role of Extracellular Calcium

Radan Stojanovic, Zoran Todorovic, Zorica Nesic, Sonja Vuckovic, Natasa Cerovac-Cosic, Milica Prostran

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Keywords: aminophylline, calcium, N^G-nitro-L-arginine methyl ester

JOURNAL FREE ACCESS

2004 Volume 96 Issue 4 Pages 493-498

DOI https://doi.org/10.1254/jphs.SCE04001X

View "Advance Publication" version (December 15, 2004).

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Abstract

The role of extracellular calcium in the interaction between intracellular cAMP and nitric oxide (NO)/cGMP on the contractility of rat diaphragm pretreated with cumulative concentrations of aminophylline (0.36 – 3.60 mM) was investigated. In a Ca²⁺-free medium, N^G-nitro-L-arginine methyl ester (L-NAME) (1 and 3 mM) depressed tension developed (Td) and also aminophylline-induced potentiation of Td in a concentration-dependent manner. Verapamil (2.5 μM) or nicardipine (20 μM) significantly antagonized the potentiating effect of L-NAME on Td in a calcium-containing medium. However, in the presence of verapamil or nicardipine, L-NAME still produced statistically significant potentiation of the cumulative concentrations of aminophylline (0.36 – 3.60 mM), given in the second series.

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