Functional Analysis of Human Sodium-Phosphate Transporter 4 (NPT4/SLC17A3) Polymorphisms

Promsuk Jutabha; Naohiko Anzai; Toru Kimura; Atsuo Taniguchi; Wako Urano; Hisashi Yamanaka; Hitoshi Endou; Hiroyuki Sakurai

doi:10.1254/jphs.10228SC

Short Communications

Functional Analysis of Human Sodium-Phosphate Transporter 4 (NPT4/SLC17A3) Polymorphisms

Promsuk Jutabha, Naohiko Anzai, Toru Kimura, Atsuo Taniguchi, Wako Urano, Hisashi Yamanaka, Hitoshi Endou, Hiroyuki Sakurai

Author information

Promsuk Jutabha
Department of Pharmacology and Toxicology, Kyorin University School of Medicine, Japan
Naohiko Anzai
Department of Pharmacology and Toxicology, Kyorin University School of Medicine, Japan
Toru Kimura
Department of Pharmacology and Toxicology, Kyorin University School of Medicine, Japan
Atsuo Taniguchi
Institute of Rheumatology, Tokyo Women’s Medical University, Japan
Wako Urano
Institute of Rheumatology, Tokyo Women’s Medical University, Japan
Hisashi Yamanaka
Institute of Rheumatology, Tokyo Women’s Medical University, Japan
Hitoshi Endou
Department of Pharmacology and Toxicology, Kyorin University School of Medicine, Japan
Hiroyuki Sakurai
Department of Pharmacology and Toxicology, Kyorin University School of Medicine, Japan

Keywords: single nucleotide polymorphism, diuretic, urate

JOURNAL FREE ACCESS

2011 Volume 115 Issue 2 Pages 249-253

DOI https://doi.org/10.1254/jphs.10228SC

View "Advance Publication" version (January 26, 2011).

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Abstract

We analyzed the functional properties of five nonsynonymous single nucleotide polymorphisms (SNPs) in the sodium-phosphate transporter NPT4 gene (SLC17A3) using the Xenopus oocyte expression system. NPT4 variants carrying SNP V257F, G279R, or P378L exhibited reduced transport of [¹⁴C]para-aminohippurate, [³H]bumetanide, [³H]estrone sulfate, and [¹⁴C]urate, when each variant clone was expressed in the plasma membrane of oocytes. This study suggests the possibility that the genetic variation of NPT4 contributes to inter-individual differences in disposition of anionic drugs such as diuretics as well as certain endogenous organic anions such as urate.

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