Acetyl shengmanol xyloside (5) and 24-O-acetylhydroshengmanol xyloside (6) were isolated from the rhizome of Cimicifuga dahurica. Shengmanol xyloside (1) was detected in the rhizome by high performance liquid chromatography (HPLC). It was found that the total content of these glycosides was 0.56% in the rhizome from the result of quantitative determination with HPLC. The above glycosides contents were determined to be 0.29% and 0.43% in the fresh rhizomes of C. acerina and C. acerina var. peltata by the same method. Cimigenol xyloside (4), 25-O-acetylcimigenol xyloside (2), and 25-O-methylcimigenol xyloside (3) seem not to be present in the fresh rhizomes of these Cimicifuga plants.