Four new triterpene glycosides, named nigellosides A, B, C, and D, were from the air-dried aerial parts of Nigella damascena L. (Ranunculaceae), and the structures were elucidated on the basis of spectroscopic data including 2D NMR spectra and chemical evidence. Their chemical structures have been characterized as 3-O-α-L-rhamnopyranosyl-(1→2)-α-L-arabinopyranosyl gypsogenin 28-β-D-glucopyranosyl-(1→6)-β-D-glucopyranosyl ester, 3-O-β-D-glucopyranosyl-(1→3)-α-L-rhamnopyranosyl-(1→2)-α-L-arabinopyranosyl gypsogenin 28-β-D-glucopyranosyl-(1→6)-β-D-glucopyranosyl ester, 3-O-α-L-rhamnopyranosyl-(1→2)-β-D-xylopyranosyl hederagenin 28-β-D-glucopyranosyl-(1→6)-β-D-glucopyranosyl ester, and 3-O-β-D-glucopyranosyl-(1→3)-α-L-rhamnopyranosyl-(1→2)-β-D-xylopyranosyl hederagenin 28-β-D-glucopyranosyl-(1→6)-β-D-glucopyranosyl ester.