Fractionation monitored by the immunomodulatory activity of the AcOEt extract of an Ascomycete, Emericella aurantio-brunnea, afforded two known fungal sesterterpenes, variecolin (1) and variecolactone (2), two new variecolin congeners named variecoacetals A (3) and B (4), and a new sesquiterpenetriol diester named emeremophiline (5), as the immunosuppressive constituests of this fungus. The absolute configuration of 1, which was previously not determined, was determined to be (2S, 3S, 6R, 10S, 11R, 14S, 15R, 16S) from the NMR spectral data of the (6R, 7R)-dimethyl-1, 3, 5-trioxacycloheptyl derivative of 1 (7). The absolute configurations of the other variecolin congeners, 2-4, and variecolol (6) are also proposed form biosynthetic considerations.