Biological and Pharmaceutical Bulletin
Online ISSN : 1347-5215
Print ISSN : 0918-6158
ISSN-L : 0918-6158
Notes
Girolline, an Antitumor Compound Isolated from a Sponge, Induces G2/M Cell Cycle Arrest and Accumulation of Polyubiquitinated p53
Sachiko TsukamotoKatsumi YamashitaKazuhiro TaneRyoichi KizuTomihisa OhtaShigeki MatsunagaNobuhiro FusetaniHiroyuki KawaharaHideyoshi Yokosawa
Author information
JOURNAL FREE ACCESS

2004 Volume 27 Issue 5 Pages 699-701

Details
Abstract

Girolline, an antitumor compound isolated from a sponge, has been reported to inhibit the termination step of protein synthesis in vivo. In this study, we found that girolline induced G2/M cell cycle arrest in several tumor cell lines. Immunochemical analysis revealed that polyubiquitinated p53 was accumulated in girolline-treated cells, while other polyubiquitinated cellular proteins were not accumulated, indicating that the effect of girolline is specific for p53. On the other hand, girolline did not inhibit proteasome activity in vitro, and accumulation of polyubiquitinated p53 was scarcely detected in the presence of leptomycin B, an inhibitor of nuclear export. Based on the above findings, we propose that girolline affects the step of recruitment of polyubiquitinated p53 to the proteasome.

Content from these authors
© 2004 The Pharmaceutical Society of Japan
Previous article Next article
feedback
Top