Cytotoxic activities of 20 steroidal glucosides obtained from Solanum genera plants were examined against various cell lines to provide new evidence as follows : 1) As regarding the sugar linkage, the glucosides possessing a β-chacotriosyl moiety were the most effective and the presence of the terminal rhamnosyl residue was required for exhibiting strong activity. 2) As for the aglycone, the glycosides carrying a spirostanol aglycone showed the strongest activity and even an aglycone without a sugar linkage presented considerably strong activity.