Skip to main content

Advertisement

SpringerLink
Log in

An Investigation into the Serum Thioredoxin, Superoxide Dismutase, Malondialdehyde, and Advanced Oxidation Protein Products in Patients with Breast Cancer

  • Breast Oncology
  • Published:
Annals of Surgical Oncology Aims and scope Submit manuscript

Abstract

Background

Reactive oxygen species (free radicals) play an important role in carcinogenesis. Extensive antioxidant defense mechanisms counteract free radicals in mammalian cells. Oxidative stress is a disturbance in the balance between the production of free radicals and antioxidant defenses. There is direct evidence that oxidative stress and lipid peroxidation (LPO) are linked to the etiology of breast cancer. The increasing global incidence of breast cancer emphasizes the need to understand the various mechanisms involved in breast tumorigenesis. The present study was undertaken to investigate the oxidative stress and antioxidant status in the blood samples of patients with breast cancer.

Methods

The present study was based on 23 women who were surgically treated at Gazi University, Faculty of Medicine, Department of General Surgery. The malondialdehyde (MDA) levels as an index of LPO along with the examination of superoxide dismutase (SOD) activities and advanced oxidation protein product (AOPP) and thioredoxin (Trx) levels were determined in the blood samples of 23 patients with breast cancer and 13 healthy controls.

Results

MDA, AOPP, and Trx levels and SOD activities were significantly higher in patients with breast cancer than the controls.

Conclusions

The results showed that oxidative stress may be related to breast cancer and especially some molecules, such as Trx and AOPP, may be useful biomarkers in breast cancer diagnosis and treatment. More detailed knowledge related to the pathophysiology of these molecules could provide valuable information on the origin and development of malignant tumors, such as breast cancer.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Latest world cancer statistics. The online GLOBOCAN (2012). http://globocan.iarc.fr/.

  2. Ziech D, Franco R, Pappa A, Panayiotidis MI. Reactive oxygen species (ROS)-induced genetic and epigenetic alterations in human carcinogenesis. Mutat Res. 2011;711:167–73.

    Article  CAS  PubMed  Google Scholar 

  3. Prescott J, Wentzensen IM, Savage SA, De Vivo I. Epidemiologic evidence for a role of telomere dysfunction in cancer etiology. Mutat Res. 2012;730:75–84.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  4. Vera-Ramirez L, Sanchez-Rovira P, Ramirez-Tortosa MC, et al. Free radicals in breast carcinogenesis, breast cancer progression and cancer stem cells. Biological bases to develop oxidative-based therapies. Crit Rev Oncol Hematol. 2011;80:347–68.

    Article  PubMed  Google Scholar 

  5. Huang TT, Zou Y, Corniola R. Oxidative stress and adult neurogenesis—effects of radiation and superoxide dismutase deficiency. Semin Cell Dev Biol. 2012;23:738–44.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  6. Powis G, Montfort WR. Properties and biological activities of thioredoxins. Ann Rev Pharmacol Toxicol. 2001;41:261–95.

    Article  CAS  Google Scholar 

  7. Gasdaska PY, Oblong JE, Cotgreave IA, Powis G. The predicted amino acid sequence of human thioredoxin is identical to that of the autocrine growth factor human adult T-cell derived factor (ADF): thioredoxin mRNA is elevated in some human tumors. Biochim Biophys Acta. 1994;1218:292–6.

    Article  CAS  PubMed  Google Scholar 

  8. Luthman M, Holgren A. Rat liver thioredoxin and thioredoxin reductase: purification and characterization. Biochemistry. 1982;21:6628–33.

    Article  CAS  PubMed  Google Scholar 

  9. Powis G, Oblong JE, Gasdaska PY, Berggren M, Hill S, Kirkpatrick DL. The thioredoxin/thioredoxin reductase redox system and control of cell growth. Oncol Res. 1994;6:539–44.

    CAS  PubMed  Google Scholar 

  10. Yoo MH, Carlson BA, Gladyshev VN, Hatfield DL. Abrogated thioredoxin system causes increased sensitivity to TNF-α-induced apoptosis via enrichment of p-ERK 1/2 in the nucleus. PLOS One. 2013;8(9):e71427. doi:10.1371/journal.pone.0071427.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  11. Nordberg J, Arner ES. Reactive oxygen species, antioxidants, and the mammalian thioredoxin system. Free Radic Biol Med. 2001;31:1287–12.

    Article  CAS  PubMed  Google Scholar 

  12. Yoshioka T, Kawada K, Shimada T, Mori M. Lipid peroxidation in maternal and cord blood protective mechanism against activated-oxygen toxicity in the blood. Am J Obstet Gynecol. 1979;135:372–6.

    CAS  PubMed  Google Scholar 

  13. Sun Y, Oberley LW, Li Y. A simple method for clinical assay of superoxide dismutase. Clin Chem. 1988;34:497–500.

    CAS  PubMed  Google Scholar 

  14. Witko-Sarsat V, Friedlander M, Capeillere-Blandin C, et al. Advanced oxidation protein products as a novel marker of oxidative stress in uremia. Kidney Int. 1996;49:1304–13.

    Article  CAS  PubMed  Google Scholar 

  15. Klaunig JE, Kamendulis LM, Hocevar BA. Oxidative stress and oxidative damage in carcinogenesis. Toxicol Pathol. 2010;38:96–109.

    Article  CAS  PubMed  Google Scholar 

  16. Cooke MS, Evans MD, Dizdaroğlu M, Lunec J. Oxidative DNA damage: mechanisms, mutation, and disease. FASEB J. 2003;10:1195–214.

    Article  Google Scholar 

  17. Gupta RK, Patel AK, Kumari R, Chugh S, Shrivastav C, Mehra S, Sharma AN. Interactions between oxidative stress, lipid profile and antioxidants in breast cancer: a case control study. Asian Pac J Cancer Prev. 2012;13:6295–8.

    Article  PubMed  Google Scholar 

  18. Gonenc A, Ozkan Y, Torun M, Simsek B. Plasma malondialdehyde (MDA) levels in breast and lung cancer patients. J Clin Pharm Ther. 2001;26:141–4.

    Article  CAS  PubMed  Google Scholar 

  19. Yeh CC, Hou MF, Tsai SM, et al. Superoxide anion radical, lipid peroxides and antioxidant status in the blood of patients with breast cancer. Clin Chim Acta. 2005;361:104–11.

    Article  CAS  PubMed  Google Scholar 

  20. Rajneesh CP, Manimaran A, Sasikala KR, Adaikappan P. Lipid peroxidation and antioxidant status in patients with breast cancer. Singapore Med J. 2008;49:640–3.

    CAS  PubMed  Google Scholar 

  21. Polat FM, Taysi S, Gul M. Oxidant/antioxidant status in blood of patients with malignant breast tumor and benign breast disease. Cell Biochem Funct. 2002;20:327–31.

    Article  CAS  PubMed  Google Scholar 

  22. Ray G, Batras S, Shukla NK. Lipid peroxidation, free radical production and antioxidant status in tissues of breast cancer. Breast Cancer Res Treat. 2000;59:163–70.

    Article  CAS  PubMed  Google Scholar 

  23. Oytun P, Ozay O, Mine EI. Coenzyme Q10 concentrations and antioxidant status in tissues of breast cancer patients. Clin Biochem. 2000;33:279–84.

    Article  Google Scholar 

  24. Capeillere-Blandin C, Gausson V, Descamps-Latscha B, Witko-Sarsat V. Biochemical and spectrophotometric significance of advanced oxidized protein products. Biochim Biophys Acta. 2004;1689:91–102.

    Article  CAS  PubMed  Google Scholar 

  25. Kaneda H, Taguchi J, Ogasawara K, Aizawa T, Ohno M. Increased level of advanced oxidation protein products in patients with coronary artery disease. Atherosclerosis. 2002;162:221–5.

    Article  CAS  PubMed  Google Scholar 

  26. Lazarova M, Stejskal D, Lacnak B, et al. The antioxidant acetylcysteine reduces oxidative stress by decreasing level of AOPPs. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2004;148:131–3.

    Article  CAS  PubMed  Google Scholar 

  27. Kosova F, Çetin B, Akıncı M, et al. Advanced oxidation protein products, ferrous oxidation in xylenol orange, and malondialdehyde levels in thyroid cancer. Endocrine Tumors. 2007;14:2616–20.

    Google Scholar 

  28. Park BJ, Cha MK, Kim IH. Thioredoxin 1 as a serum marker for breast cancer and its use in combination with CEA or CA15-3 for improving the sensitivity of breast cancer diagnoses. BMC Res Notes. 2014;7:7. doi:10.1186/1756-0500-7-7.

    Article  PubMed Central  PubMed  Google Scholar 

  29. Ueno M, Matsutani Y, Nakamura H, et al. Possible association of thioredoxin and p53 in breast cancer. Immunol Lett. 2000;75:15–20.

    Article  CAS  PubMed  Google Scholar 

  30. Lincoln DT, Ali Amadi LM, Tonissen KF, Clarke FM. The thioredoxin–thioredoxin reductase system: over-expression in human cancer. Anticancer Res. 2003;23:2425–33.

    CAS  PubMed  Google Scholar 

Download references

Acknowledgment

This study was supported by grants from Gazi University Scientific Research Projects Department.

Author information

Authors and Affiliations

  1. Faculty of Medicine, Department of Medical Biochemistry, Gazi University, Ankara, Turkey

    Nedret Kilic PhD

  2. Department of Clinical Biochemistry, Diskapi Yildirim Beyazit Training and Research Hospital, Etlik, Ankara, Turkey

    Mine Yavuz Taslipinar MD

  3. Department of Radiation Oncology, Dr. Abdurrahman Yurtaslan Ankara Oncology Training and Research Hospital, Ankara, Turkey

    Yildiz Guney PhD, MD

  4. Department of General Surgery, Gazi University Faculty of Medicine, Ankara, Turkey

    Ercument Tekin MD & Erhan Onuk MD

Authors
  1. Nedret Kilic PhD
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Mine Yavuz Taslipinar MD
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yildiz Guney PhD, MD
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ercument Tekin MD
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Erhan Onuk MD
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Mine Yavuz Taslipinar MD.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Kilic, N., Yavuz Taslipinar, M., Guney, Y. et al. An Investigation into the Serum Thioredoxin, Superoxide Dismutase, Malondialdehyde, and Advanced Oxidation Protein Products in Patients with Breast Cancer. Ann Surg Oncol 21, 4139–4143 (2014). https://doi.org/10.1245/s10434-014-3859-3

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1245/s10434-014-3859-3

Keywords

Search

Navigation