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p53, BCL-2, and Ki-67 Expression According to Tumor Response After Concurrent Chemoradiotherapy for Advanced Rectal Cancer

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Abstract

Background: Concurrent chemoradiotherapy (CCRT) for locally advanced rectal cancer is an important modality for curative resection, but tumors show wide spectrum response. The purpose of this study was to investigate any correlation among related genetic mutations, proliferative index, and tumor response after CCRT.

Methods: This study included 23 patients with rectal cancer, who were preoperatively staged as at least T3 N1 or T4 (determined by transrectal ultrasonography and MRI). Enrolled patients were given 5-FU 450 mg/m2/day and leucovorin 20 mg/m2/day intravenously for 5 days during weeks 1 and 5 of radiotherapy (45–54 Gy). Surgical resection was performed 4 weeks after completion of the scheduled treatment. Tumor response was classified as CR (complete response), PR (partial response: 50% diminution of tumor volume and downstaging), and NR (no response). Paraffin-embedded tissue obtained before chemoradiotherapy was studied by immunohistochemical staining for p53, BCL-2, and Ki-67. The extent of tumor response was correlated with proliferative activity and was measured by immunostaining Ki-67 proliferative antigen and the expression of p53 and BCL-2 oncoproteins.

Results: All patients were resectable. CR was obtained in 4 patients, PR in 10 patients, and NR in 9 patients. The p53 mutation was noted in 16 patients: NR in 5 patients, PR in 9 patients, and CR in 2 patients (P = .638). BCL-2 expression was noted in 11 patients: NR in 4 patients, PR in 3 patients, and CR in 4 patients (P = .799). The Ki-67 labeling index was NR: 615.4 ± 47.2; PR: 663.2 ± 20.4; and CR: 765.5 ± 58.3 (CR + PR vs. NR, P = .029).

Conclusions: Immunohistochemical expression of p53 and BCL-2 does not correlate with tumor response after CCRT, but Ki-67 labeling may be a useful parameter for radiosensitive tumors selected for CCRT.

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Authors and Affiliations

  1. Departments of Surgery, Yonsei University College of Medicine, Seoul, Korea

    Nam Kyu Kim MD, Jae Kun Park MD, Kang Yong Lee MD, Seong Hyeon Yun MD & Jin Sik Min MD

  2. Departments of Pathology, Yonsei University College of Medicine, Seoul, Korea

    Woo IK Yang MD

  3. Departments of Radiation Oncology, Yonsei University College of Medicine, Seoul, Korea

    JinSil Sung MD

  4. Department of Surgery, Yonsei University College of Medicine, Seodamun-Gu, Shinchon-dong 134, CPO 8044, Seoul, Korea

    Nam Kyu Kim MD

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  1. Nam Kyu Kim MD
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  2. Jae Kun Park MD
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  6. JinSil Sung MD
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  7. Jin Sik Min MD
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Correspondence to Nam Kyu Kim MD.

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Kim, N.K., Park, J.K., Lee, K.Y. et al. p53, BCL-2, and Ki-67 Expression According to Tumor Response After Concurrent Chemoradiotherapy for Advanced Rectal Cancer. Ann Surg Oncol 8, 418–424 (2001). https://doi.org/10.1007/s10434-001-0418-5

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  • DOI: https://doi.org/10.1007/s10434-001-0418-5

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