Preliminary study on effect of caspofungin on biofilm forming ability of Enterococcus faecalis
BAI Bing, LIN Zhi-wei, XU Guang-jian, SUN Xiang, CHEN Jun-wen, TU Hao-peng, LI Duo-yun, YU Zhi-jian, DENG Qi-wen, ZHENG Jin-xin
Department of Infectious Diseases and Shenzhen Key Laboratory for Endogenous Infection, Shenzhen Nanshan Hospital of Shenzhen University, Shenzhen 518052, China
Abstract：Objective To analyze the effect of caspofungin on inhibiting the formation of Enterococcus faecalis(E. faecalis) biofilm. Methods One strain of clinically isolated E. faecalis with strong positive biofilm and 10 strains of E. faecalis with different biofilm forming ability were selected, caspofungin intervention group and control group were set up at the same time, intervention group used caspofungin with the concentration of 20 μmol/L, control group used normal saline. Growth of planktonic strains and biofilm formation of E. faecalis in two groups were detected by crystal violet staining and microplate reader. Results Caspofungin had no inhibitory effect on the growth of planktonic clinical E. faecalis strains, but can significantly inhibit the formation of biofilm. Conclusion Caspofungin has a significant inhibitory effect on biofilm formation of E. faecalis.
白冰, 林志伟, 徐广健, 孙翔, 陈俊文, 涂浩鹏, 李多云, 余治健, 邓启文, 郑金鑫. 卡泊芬净对粪肠球菌生物膜形成能力影响的初步研究[J]. 中国感染控制杂志, 2019, 18(7): 607-611.
BAI Bing, LIN Zhi-wei, XU Guang-jian, SUN Xiang, CHEN Jun-wen, TU Hao-peng, LI Duo-yun, YU Zhi-jian, DENG Qi-wen, ZHENG Jin-xin. Preliminary study on effect of caspofungin on biofilm forming ability of Enterococcus faecalis. Chinese Journal of Infection Control, 2019, 18(7): 607-611.
Garg S, Mohan B, Taneja N. Biofilm formation capability of enterococcal strains causing urinary tract infection vis-a-vis colonisation and correlation with enterococcal surface protein gene[J]. Indian J Med Microbiol, 2017, 35(1):48-52.
[2]
Vargas-Cruz N, Reitzel RA, Rosenblatt J, et al. In vitro study of antimicrobial percutaneous nephrostomy catheters for prevention of renal infections[J]. Antimicrob Agents Chemother, 2017, 61(6):1-6.
[3]
Zaborowska M, Tillander J, Brånemark R, et al. Biofilm formation and antimicrobial susceptibility of staphylococci and enterococci from osteomyelitis associated with percutaneous orthopaedic implants[J]. J Biomed Mater Res B Appl Biomater, 2017, 105(8):2630-2640.
[4]
Zheng JX, Bai B, Lin ZW, et al. Characterization of biofilm formation by Enterococcus faecalis isolates derived from urinary tract infections in China[J]. J Med Microbiol, 2018, 67:60-67.
[5]
Zheng JX, Wu Y, Lin ZW, et al. Characteristics of and virulence factors associated with biofilm formation in clinical Enterococcus faecalis isolates in China[J]. Front Microbiol, 2017, 8:2338.
[6]
Arias CA, Murray BE. The rise of the Enterococcus:beyond vancomycin resistance[J]. Nat Rev Microbiol, 2012, 10(4):266-278.
[7]
Jones ME, Draghi DC, Thornsberry C, et al. Emerging resistance among bacterial pathogens in the intensive care unit-a European and North American Surveillance study (2000-2002)[J]. Ann Clin Microbiol Antimicrob, 2004, 3:14.
[8]
O'Driscoll T, Crank CW, Crank. Vancomycin-resistant enterococcal infections:epidemiology, clinical manifestations, and optimal management[J]. Infect Drug Resist, 2015, 8:217-230.
[9]
Siala W, Kucharíková S, Braem A, et al. The antifungal caspofungin increases fluoroquinolone activity against Staphylococcus aureus biofilms by inhibiting N-acetylglucosamine transferase[J]. Nat Commun, 2016, 7:13286.
[10]
Mohamed JA, Huang W, Nallapareddy SR, et al. Influence of origin of isolates, especially endocarditis isolates, and various genes on biofilm formation by Enterococcus faecalis[J]. Infect Immun, 2004, 72(6):3658-3663.
[11]
Lebeaux D, Ghigo JM, Beloin C, et al. Biofilm-related infections:bridging the gap between clinical management and fundamental aspects of recalcitrance toward antibiotics[J]. Microbiol Mol Biol Rev, 2014, 78(3):510-543.
[12]
Tong Z, Zhang Y, Ling J, et al. An in vitro study on the effects of nisin on the antibacterial activities of 18 antibiotics against Enterococcus faecalis[J]. PLoS One, 2014, 9(2):e89209.
[13]
Tang HJ, Chen CC, Zhang CC, et al. In vitro efficacy of fosfomycin-based combinations against clinical vancomycin-resis-tant Enterococcus isolates[J]. Diagn Microbiol Infect Dis, 2013, 77(3):254-257.
[14]
Oliva A, Furustrand Tafin U, Maiolo EM, et al. Activities of fosfomycin and rifampin on planktonic and adherent Enterococcus faecalis strains in an experimental foreign-body infection model[J]. Antimicrob Agents Chemother, 2014, 58(3):1284-1293.
[15]
Luther MK, Arvanitis M, Mylonakis E, et al. Activity of daptomycin or linezolid in combination with rifampin or gentamicin against biofilm-forming Enterococcus faecalis or E. faecium in an in vitro pharmacodynamic model using simulated endocardial vegetations and an in vivo survival assay using Galleria mellonella larvae[J]. Antimicrob Agents Chemother, 2014, 58(8):4612-4620.
[16]
Safdar A, Rodriguez G, Zuniga J, et al. High-dose caspofungin as a component of combination antifungal therapy in 91 patients with neoplastic diseases and hematopoietic stem cell transplantation:a critical review of short-term and long-term adverse events[J]. J Pharm Pract, 2015, 28(2):175-182.
[17]
Bachmann SP, VandeWalle K, Ramage G, et al. In vitro activity of caspofungin against Candida albicans biofilms[J]. Antimicrob Agents Chemother, 2002, 46(11):3591-3596.
[18]
Uppuluri P, Srinivasan A, Ramasubramanian A, et al. Effects of fluconazole, amphotericin B, and caspofungin on Candida albicans biofilms under conditions of flow and on biofilm dispersion[J]. Antimicrob Agents Chemother, 2011, 55(7):3591-3593.
[19]
Maiolo EM, Furustrand Tafin U, Borens O, et al. Activities of fluconazole, caspofungin, anidulafungin, and amphotericin B on planktonic and biofilm Candida species determined by microcalorimetry[J]. Antimicrob Agents Chemother, 2014, 58(5):2709-2717.
[20]
Vriens K, Cools TL, Harvey PJ, et al. Synergistic activity of the plant defensin HsAFP1 and caspofungin against Candida albicans biofilms and planktonic cultures[J]. PLoS One, 2015, 10(8):e0132701.
