Abstract
In order to investigate the correlation among energy input–related, drug-related, and stabilizer-related aspects for both top-down and bottom-up nanocrystal production, meloxicam nanosuspensions (NS) were produced by using three different methods (low-energy wet milling, high-pressure homogenization, and precipitation) and each method was optimized by using design of experiment (DoE). Box-Behnken design of 3 factors and 3 levels was applied for the optimization of each method. All the three models were found to be significant and the optimized process parameters were used for production of NS, respectively. Interestingly, by comparison of the top-down and bottom-up approaches, the influence of energy input (homogenization pressure or milling speed) from the instruments seemed not significant for top-down compared with bottom-up for this drug. Different mechanisms of homogenization (relatively high energy zone) and milling (relatively low energy zone) led to obtained various significant correlations for each method. Capsules containing nanocrystals were successfully produced by using a novel method applying NS (after wet bead milling and homogenization processes) as wetting agent for direct capsuling and showed superiority regarding as dissolution rate compared with the traditional two-step method (freeze-dried powder used for capsuling as the first step). Different NS preparation methodologies proved to have a direct influence on the following capsuling process and consequently, in the dissolution rate. This study also proved that residual DMSO in nanosuspension after precipitation process could affect the freeze-drying process, which might further alter the redispersion and influence the downstream processes.
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References
Li M, Alvarez P, Bilgili E. A microhydrodynamic rationale for selection of bead size in preparation of drug nanosuspensions via wet stirred media milling. Int J Pharm. 2017;524(1–2):178–92. https://doi.org/10.1016/j.ijpharm.2017.04.001.
Liu T, Muller RH, Moschwitzer JP. Effect of drug physico-chemical properties on the efficiency of top-down process and characterization of nanosuspension. Expert Opin Drug Deliv. 2015;12(11):1741–54. https://doi.org/10.1517/17425247.2015.1057566.
Pensel P, Paredes A, Albani CM, Allemandi D, Sanchez Bruni S, Palma SD, et al. Albendazole nanocrystals in experimental alveolar echinococcosis: Enhanced chemoprophylactic and clinical efficacy in infected mice. Vet Parasitol. 2018:78–84.
Castro SG, Bruni SFS, Urbizu LP, Confalonieri A, Ceballos L, Lanusse CE, et al. Enhanced dissolution and systemic availability of albendazole formulated as solid dispersions. Pharm Dev Technol. 2013;18(2):434–42. https://doi.org/10.3109/10837450.2012.693509.
Paredes AJ, Bruni SS, Allemandi D, Lanusse C, Palma SD. Albendazole nanocrystals with improved pharmacokinetic performance in mice. Ther Deliv. 2018;9(2):89–97. https://doi.org/10.4155/tde-2017-0090.
Sattar A, Chen D, Jiang L, Pan Y, Tao Y, Huang L, et al. Preparation, characterization and pharmacokinetics of cyadox nanosuspension. Sci Rep. 2017;7(1):2289. https://doi.org/10.1038/s41598-017-02523-4.
Liu T, Yao GL, Liu XT, Yin HP. Preparation nanocrystals of poorly soluble plant compounds using an ultra-small-scale approach. AAPS PharmSciTech. 2017;18(7):2610–7. https://doi.org/10.1208/s12249-017-0742-0.
Attari Z, Bhandari A, Jagadish PC, Lewis S. Enhanced ex vivo intestinal absorption of olmesartan medoxomil nanosuspension: preparation by combinative technology. Saudi Pharm J. 2016;24(1):57–63. https://doi.org/10.1016/j.jsps.2015.03.008.
Paredes AJ, Llabot JM, Bruni SS, Allemandi D, Palma SD. Self-dispersible nanocrystals of albendazole produced by high pressure homogenization and spray-drying. Drug Dev Ind Pharm. 2016;42(10):1564–70. https://doi.org/10.3109/03639045.2016.1151036.
Medarevic D, Djuris J, Ibric S, Mitric M, Kachrimanis K. Optimization of formulation and process parameters for the production of carvedilol nanosuspension by wet media milling. Int J Pharm. 2018;540(1–2):150–61. https://doi.org/10.1016/j.ijpharm.2018.02.011.
Sahibzada MUK, Sadiq A, Faidah HS, Khurram M, Amin MU, Haseeb A, et al. Berberine nanoparticles with enhanced in vitro bioavailability: characterization and antimicrobial activity. Drug Des Devel Ther. 2018;12:303–12. https://doi.org/10.2147/DDDT.S156123.
Kakran M, Sahoo NG, Tan IL, Li L. Preparation of nanoparticles of poorly water-soluble antioxidant curcumin by antisolvent precipitation methods. J Nanopart Res. 2012;14(3). doi: Unsp 757. https://doi.org/10.1007/S11051-012-0757-0.
Liu T, Muller RH, Moschwitzer JP. Production of drug nanosuspensions: effect of drug physical properties on nanosizing efficiency. Drug Dev Ind Pharm. 2018;44(2):233–42. https://doi.org/10.1080/03639045.2017.1386207.
dos Santos AM, Carvalho FC, Teixeira DA, Azevedo DL, de Barros WM, Gremiao MPD. Computational and experimental approaches for development of methotrexate nanosuspensions by bottom-up nanoprecipitation. Int J Pharm. 2017;524(1–2):330–8. https://doi.org/10.1016/j.ijpharm.2017.03.068.
Sinha B, Muller RH, Moschwitzer JP. Bottom-up approaches for preparing drug nanocrystals: formulations and factors affecting particle size. Int J Pharm. 2013;453(1):126–41. https://doi.org/10.1016/j.ijpharm.2013.01.019.
Liu T, Muller RH, Moschwitzer JP. Systematical investigation of a combinative particle size reduction technology for production of resveratrol nanosuspensions. AAPS PharmSciTech. 2017;18(5):1683–91. https://doi.org/10.1208/s12249-016-0612-1.
Chudiwal SS, Dehghan MH. Quality by design approach for development of suspension nasal spray products: a case study on budesonide nasal suspension. Drug Dev Ind Pharm. 2016;42(10):1643–52. https://doi.org/10.3109/03639045.2016.1160108.
Parmentier J, Tan EH, Low A, Moschwitzer JP. Downstream drug product processing of itraconazole nanosuspension: factors influencing drug particle size and dissolution from nanosuspension-layered beads. Int J Pharm. 2017;524(1–2):443–53. https://doi.org/10.1016/j.ijpharm.2017.04.012.
Sun W, Ni R, Zhang X, Li LC, Mao SR. Spray drying of a poorly water-soluble drug nanosuspension for tablet preparation: formulation and process optimization with bioavailability evaluation. Drug Dev Ind Pharm. 2015;41(6):927–33. https://doi.org/10.3109/03639045.2014.914528.
Cal K, Sollohub K. Spray drying technique. I: hardware and process parameters. J Pharm Sci. 2010;99(2):575–86. https://doi.org/10.1002/jps.21886.
Chin WWL, Parmentier J, Widzinski M, Tan EH, Gokhale R. A brief literature and patent review of nanosuspensions to a final drug product. J Pharm Sci. 2014;103(10):2980–99. https://doi.org/10.1002/jps.24098.
Colombo M, Orthmann S, Bellini M, Staufenbiel S, Bodmeier R. Influence of drug brittleness, nanomilling time, and freeze-drying on the crystallinity of poorly water-soluble drugs and its implications for solubility enhancement. AAPS PharmSciTech. 2017;18(7):2437–45. https://doi.org/10.1208/s12249-017-0722-4.
Zaman M, Hanif M, Shaheryar ZA. Development of tizanidine HCl-meloxicam loaded mucoadhesive buccal films: in-vitro and in-vivo evaluation. PLoS One. 2018;13(3):e0194410. https://doi.org/10.1371/journal.pone.0194410.
Louati K, Bargaoui I, Safta F. Development and validation of ultra-performance liquid chromatography method for the determination of meloxicam and its impurities in active pharmaceutical ingredients. Ann Pharm Fr. 2018;76(3):187–200. https://doi.org/10.1016/j.pharma.2018.02.001.
Gottlieb IJ, Tunick DR, Mack RJ, McCallum SW, Howard CP, Freyer A, et al. Evaluation of the safety and efficacy of an intravenous nanocrystal formulation of meloxicam in the management of moderate-to-severe pain after bunionectomy. J Pain Res. 2018;11:383–93. https://doi.org/10.2147/JPR.S149879.
Ochi M, Kawachi T, Toita E, Hashimoto I, Yuminoki K, Onoue S, et al. Development of nanocrystal formulation of meloxicam with improved dissolution and pharmacokinetic behaviors. Int J Pharm. 2014;474(1–2):151–6. https://doi.org/10.1016/j.ijpharm.2014.08.022.
Yu Q, Wu XY, Zhu QG, Wu W, Chen ZJ, Li Y, et al. Enhanced transdermal delivery of meloxicam by nanocrystals: preparation, in vitro and in vivo evaluation. Asian J Pharm Sci. 2018;13(6):518–26. https://doi.org/10.1016/j.ajps.2017.10.004.
Xia DN, Quan P, Piao HZ, Piao HY, Sun SP, Yin YM, et al. Preparation of stable nitrendipine nanosuspensions using the precipitation-ultrasonication method for enhancement of dissolution and oral bioavailability. Eur J Pharm Sci. 2010;40(4):325–34. https://doi.org/10.1016/j.ejps.2010.04.006.
Singh SK, Srinivasan KK, Gowthamarajan K, Singare DS, Prakash D, Gaikwad NB. Investigation of preparation parameters of nanosuspension by top-down media milling to improve the dissolution of poorly water-soluble glyburide. Eur J Pharm Biopharm. 2011;78(3):441–6. https://doi.org/10.1016/j.ejpb.2011.03.014.
Keck CM, Muller RH. Drug nanocrystals of poorly soluble drugs produced by high pressure homogenisation. Eur J Pharm Biopharm. 2006;62(1):3–16. https://doi.org/10.1016/j.ejpb.2005.05.009.
Merisko-Liversidge E, Liversidge GG. Nanosizing for oral and parenteral drug delivery: a perspective on formulating poorly-water soluble compounds using wet media milling technology. Adv Drug Deliv Rev. 2011;63(6):427–40. https://doi.org/10.1016/j.addr.2010.12.007.
Abdelwahed W, Degobert G, Stainmesse S, Fessi H. Freeze-drying of nanoparticles: formulation, process and storage considerations. Adv Drug Deliv Rev. 2006;58(15):1688–713. https://doi.org/10.1016/j.addr.2006.09.017.
Iurian S, Bogdan C, Tomuta I, Szabo-Revesz P, Chvatal A, Leucuta SE, et al. Development of oral lyophilisates containing meloxicam nanocrystals using QbD approach. Eur J Pharm Sci. 2017;104:356–65. https://doi.org/10.1016/j.ejps.2017.04.011.
Kurti L, Kukovecz A, Kozma G, Ambrus R, Deli MA, Szabo-Revesz P. Study of the parameters influencing the co-grinding process for the production of meloxicam nanoparticles. Powder Technol. 2011;212(1):210–7. https://doi.org/10.1016/j.powtec.2011.05.018.
Liu T, Muller RH, Moschwitzer JP. Consideration of the solid state for resveratrol nanocrystal production. Powder Technol. 2018;332:63–9. https://doi.org/10.1016/j.powtec.2018.03.028.
Choi J-Y, Yoo JY, Kwak H-S, Nam BU, Lee J. Role of polymeric stabilizers for drug nanocrystal dispersions. Curr Appl Phys. 2005;5(5):472–4.
Noolkar SB, Jadhav NR, Bhende SA, Killedar SG. Solid-state characterization and dissolution properties of meloxicam-Moringa coagulant-PVP ternary solid dispersions. AAPS PharmSciTech. 2013;14(2):569–77. https://doi.org/10.1208/s12249-013-9941-5.
Jafar M, Mhg D, Shareef A. Enhancement of dissolution and anti-inflammatory effect of meloxicam using solid dispersions. Int J Appl Pharm. 2010;2(1):22–7.
Siow CRS, Wan Sia Heng P, Chan LW. Application of freeze-drying in the development of oral drug delivery systems. Expert Opin Drug Deliv. 2016;13(11):1595–608. https://doi.org/10.1080/17425247.2016.1198767.
Du J, Li XG, Zhao HX, Zhou YQ, Wang LL, Tian SS, et al. Nanosuspensions of poorly water-soluble drugs prepared by bottom-up technologies. Int J Pharm. 2015;495(2):738–49. https://doi.org/10.1016/j.ijpharm.2015.09.021.
Funding
Tao Liu received financial support from A Project of Shandong Province Higher Educational Science and Technology Program (project no. J18KA269), Shandong Provincial Key R&D Program (project no. 2019GSF107006), and Qingdao Source Innovation Program (project no. 19-6-2-38-cg).
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Liu, T., Yu, X. & Yin, H. Study of Top-down and Bottom-up Approaches by Using Design of Experiment (DoE) to Produce Meloxicam Nanocrystal Capsules. AAPS PharmSciTech 21, 79 (2020). https://doi.org/10.1208/s12249-020-1621-7
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DOI: https://doi.org/10.1208/s12249-020-1621-7