Abstract
The purpose of the present study was to develop and characterize the chitosan sponges loading with doxycycline hyclate and their antibacterial activities. The pore density of chitosan sponge prepared with freeze drying technique was increased as the higher concentrated chitosan solution was used. The sponge prepared from 10% w/w of the chitosan solution and crosslinking with glutaraldehyde solution was utilized for loading with doxycycline hyclate. The drug release and sustainable antibacterial activity of fabricated sponge were assessed using dissolution test and agar diffusion test, respectively. Drug release from non-crosslinked sponge into phosphate buffer pH7.4 was slower than that from crosslinked sponge since the former could absorb the medium and form gel to retard the initial drug diffusion. Sustainable antibacterial activity of developed sponge was evident against S. aureus and E. coli. In conclusion, the in vitro release profile and antibacterial efficiency indicated that doxycycline hyclate could be sustained using chitosan sponge.
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Acknowledgments
The authors wish to thank Thailand Research Fund (Grant No. DBG5080014) for research grant support and T. Man Pharma Ltd., PART. This work was under kindly provision by Faculty of Pharmacy, Silpakorn University. The authors would like to express my appreciation to Mrs. Kamnuam Kongsupalak, Dr. Wanchai Chongcharoen, Miss Kullap Jirangkul, Mr. Suthep Sutthipongkiat and Miss Suchada Wattananawinrat for their help and comment.
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Phaechamud, T., Charoenteeraboon, J. Antibacterial Activity and Drug Release of Chitosan Sponge Containing Doxycycline Hyclate. AAPS PharmSciTech 9, 829–835 (2008). https://doi.org/10.1208/s12249-008-9117-x
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DOI: https://doi.org/10.1208/s12249-008-9117-x