Abstract
Aim: We wanted to compare two ELISA kits for detection of IgG and IgM antibodies against Borrelia in samples taken from children with suspected neuroborreliosis.
Methods: Between 01.04.2009 and 31.03.2010 serum and cerebrospinal fluid from children with various neurological symptoms were analyzed using Biomedica kits. Positive samples were confirmed with MIKROGEN Diagnostik Immunoblot assay and tested with DRG Diagnostics kits. The Biomedica IgG kit contains recombinant antigens: OspC, p18, p100 and VIsE ; the IgM Biomedica kit contains recombinant antigens: OspC (B.afzelii, B.garinii), p41/I, VIsE. The DRG IgG kit contains recombinant VIsE antigen and lysate antigens from B.burgdorferi s.s., B.afzelii and B.garinii; the DRG IgM kit contains recombinant 14 kD protein and native OspC. Interpretation of the ELISA and Immunoblot was done according to the manufacturer's instructions.
Results: A total of 379 samples (353 sera and 26 CSF) were analyzed for Borrelia antibodies. Antibodies against B.burgdorferi were detected by Biomedica kit in 55 samples from which 29 (52%) were confirmed with Immunoblot. Based on the Immunoblot analysis 29 / 379 (7,3 %) samples were considered as “true positive”. Among two ELISA tests, 26 sera were false-positive with the Biomedica test and 13 sera were false-positive with the DRG Diagnostics test. However, DRG Diagnostics test failed to detect two sera that were positive with the Biomedica test and Immunoblot.
Conclusions: We observed significant differences between the two ELISA tests. Biomedica test showed higher sensitivity but it produced more false-positive results. DRG Diagnostics test was more specific, but less sensitive.
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Pawinska, A., Vogtt, E. & Dzierzanowska-Fangrat, K. 874 Serological Diagnosis of Lyme Neuroborreliosis in Children with Various Neurological Disorders - Comparison of Two Elisa Tests. Pediatr Res 68 (Suppl 1), 439 (2010). https://doi.org/10.1203/00006450-201011001-00874
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DOI: https://doi.org/10.1203/00006450-201011001-00874