Abstract
ICSA and ICA are present in more than 90% of children at the onset of IDDM and also prior to its development. In addition 6-25% of unaffected sibs of IDDM probands also have such antibodies but most never develop IDDM. In the present study we have measured complement-dependent cytotoxic ICSA by a simple microcytotoxicity assay using as targets the cloned rat, insulinoma line RIN-m Cytotoxicity was positive if 50% of cells were killed after incubation with diluted, heat inactivated, rat liver powder-adsorbed sera followed by complement with ethidium bromide added after 90'; dead cells were identified under epiillumination with an inverted Leitz microscope. The sera of all unaffected first degree relatives of 112 IDDM probands were examined and the results were divided with reference to sharing of HLA haplotypes by the sibs with their respective diabetic probands. 23/186 (12.4%) sibs were ICSA-positive and this was most common in sibs whose diabetic sib was also ICSA-positive(32.1 vs. 3.8%, p<.001).Furthermore, ICSA were found predominantly in those sibs HLA-identical (7/44) or haploidentical (14/105) to their diabetic sib and were rare in HLA-nonidentical sibs (2/37, p<.05).
The results suggest that the tendency to produce ICSA may be inherited as a dominant trait, different from the inheritance of IDDM itself. Further studies are required to identify etiologic factors in the development of these ICSA.
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Ginsberg-Fellner, F., Taguchi, Y., Witt, M. et al. PANCREATIC ISLET CELL ANTIBODIES (ICSA) IN FAMILIES OF CHILDREN WITH TYPE I DIABETES MELLITUS (IDDM): DOMINANT INHERITANCE. Pediatr Res 18 (Suppl 4), 221 (1984). https://doi.org/10.1203/00006450-198404001-00769
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DOI: https://doi.org/10.1203/00006450-198404001-00769