ABSTRACT
A number of forward and reverse genetics methods have been developed in cereal crops where a huge sequence can be determined using reverse genetics. The forward genetic approach has proven powerful in elucidating complex processes and determining the gene-function relationship. Transposon-mediated mutagenesis is a high-powered forward genetic technique used to identify the specific genetic elements that control phenotypes. It utilizes transposable genetic elements which integrate and produce arbitrary insertion mutations that are easily identified. Transformation of T-DNA-bearing transposons normally generates a few copies; thus, transposon amplification was used to increase copy number. To understand the transposable elements from the genome of plants that assess the diversity of evolutionary adaptability in plant taxa. In the present chapter, we briefly show the mutagenesis-based approaches in cereals and summarize the genes studied in the cereals.
