Journal of Lipid Research
Volume 51, Issue 5, May 2010, Pages 1228-1236
MethodsAn optimized negative-staining protocol of electron microscopy for apoE4•POPC lipoprotein
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open access
apolipoprotein E
cryo-EM
electron microscopy
phospholipid
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This work was supported by the W. M. Keck Foundations (G.R.); in part by the National Pre-doctoral Scholarship of China through the China Scholarship Council (CSC) (File No. 2008628018) (L.Z.); and by RO1 AG028793 (K.W.) from the National Institutes of Health. Its contents are solely the responsibility of the authors and do not necessarily represent the official views of the National Institutes of Health or other granting agencies.
Abbreviations:
- apoE
apolipoprotein E
- apoE4•POPC
POPC-bound form of apoE4
- Cryo-EM
cryo-electron microscopy
- EM
electron microscopy
- FPLC
fast-protein liquid chromatography
- NS
negative staining
- POPC
palmitoyl-oleoylphosphatidylcholine
- PTA
phosphotungstic acid
- UF
uranyl formate
© 2010 ASBMB. Currently published by Elsevier Inc; originally published by American Society for Biochemistry and Molecular Biology.