基于海藻酸自组装胶体粒子的制备及其乳化性能

doi:10.11901/1005.3093.2020.455

材料研究学报

2021, Vol. 35

Issue (10): 761-768 DOI: 10.11901/1005.3093.2020.455

研究论文

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基于海藻酸自组装胶体粒子的制备及其乳化性能

张翠歌(

), 胡良, 卢祖新, 周佳慧

安徽工程大学化学与环境工程学院芜湖 241000

Preparation and Emulsification Properties of Self-assembled Colloidal Particles Based on Alginic Acid

ZHANG Cuige(

), HU Liang, LU Zuxin, ZHOU Jiahui

School of Chemical and Environmental Engineering, Anhui Polytechnic University, Wuhu 241000, China

引用本文:

张翠歌, 胡良, 卢祖新, 周佳慧. 基于海藻酸自组装胶体粒子的制备及其乳化性能[J]. 材料研究学报, 2021, 35(10): 761-768.
Cuige ZHANG, Liang HU, Zuxin LU, Jiahui ZHOU. Preparation and Emulsification Properties of Self-assembled Colloidal Particles Based on Alginic Acid[J]. Chinese Journal of Materials Research, 2021, 35(10): 761-768.

全文: PDF(5129 KB) HTML

摘要:

用海藻酸(ALG)和溶菌酶(Lys)静电自组装制备胶体粒子，用纳米粒度仪和透射电镜对其尺寸和形貌进行表征，研究了溶菌酶和海藻酸的质量比(WR)对胶体粒子性质的影响，得到了具有最佳WR的胶体粒子。胶体粒子具有球形结构，粒径约为143 nm。这种胶体粒子可二次组装在油水界面稳定水包油型Pickering乳液，研究了pH值和盐浓度对胶体粒子性质和乳化性能的影响。随着pH值的增大胶体粒子和乳液滴粒径均逐渐增大而乳化性能逐渐降低; 随着盐浓度的提高胶体粒子和乳液滴的粒径先减小后增大，乳化性能先提高后降低。乳液均保留了一定的活性。

关键词 ：有机高分子材料, Pickering 乳液, 自组装, 酶活

Abstract：

Self-assembled colloidal particles were prepared via electrostatic interaction between alginic acid (ALG) and lysozyme (Lys), and the effect of the mass ratio of lysozyme to alginic acid on the properties of colloidal particles was investigated in order to obtain colloidal particles at the optimal mass ratio. The size and morphology of colloidal particles were characterized by nanometer particle size analyzer and transmission electron microscope. The results show that the colloidal particles are spherical with particle size of about 143 nm. The colloidal particles can be assembled at the oil-water interface to stabilize the oil-in-water Pickering emulsion. The effects of pH value and salt concentration on the properties and the emulsifying properties of colloidal particles were investigated in detail. The results show that as the pH value increases, the colloidal particles and emulsion droplet size both gradually increase, and the emulsification performance gradually decreases; as the salt concentration increases the colloidal particles and emulsion droplet size first decreases and then increases, while the emulsification performance increases first and then decreases. The effect of pH and salt concentration on the activity of lysozyme in the emulsion was further investigated. It follows that the prepared emulsions are all active to a certain extent .

Key words： organic polymer materials pickering emulsion self-assembly enzyme activity

收稿日期: 2020-10-29

ZTFLH:

O636.1

基金资助:国家自然科学基金(51703001);安徽省教育厅自然科学基金(KJ2017A115);安徽省大学生创新创业训练计划(S202010363259)

作者简介: 张翠歌，女，1977年生，博士

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https://www.cjmr.org/CN/10.11901/1005.3093.2020.455 或 https://www.cjmr.org/CN/Y2021/V35/I10/761

图1 Lys/ALG胶体粒子的制备及油水界面二次组装示意图

图2 Lys/ALG胶体粒子的粒径，ζ电位和多分散度与质量比WR的关系

图3 Lys/ALG胶体粒子的粒径分布、表观照片和TEM图

图4 胶体粒子粒径和ζ电位与pH值的关系

图5 不同pH值乳液的数码照片和乳液滴的显微镜照片、乳液滴平均粒径和多分散度与pH值的关系以及乳液滴平均粒径随时间的变化

图6 胶体粒子粒径和ζ电位与盐浓度的关系

图7 不同盐浓度乳液的数码照片和乳液滴的显微镜照片、乳液滴平均粒径和多分散度与盐浓度的关系以及乳液滴平均粒径随时间的变化

图8 不同油水比胶体粒子的乳化性能：乳液的数码照片和乳液滴的显微镜照片以及乳液滴的平均尺寸和多分散度

图9 自由溶菌酶，Lys/ALG胶体粒子和胶体粒子制备的乳液的溶菌酶的活性

图10 不同pH乳液中溶菌酶的活性和不同盐浓度乳液中溶菌酶的活性，pH 值为5.9

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