| 基于铈配合物信号探针的凝血酶电化学适体传感器 | |
| Electrochemical aptamer sensor for thrombin based on cerium complex signal probe | |
| 摘要: 以Ce3+为中心离子，N，N-二甲基甲酰胺(DMF)为有机配体，通过温度调节，合成系列形貌和电化学信号不同的铈配合物(Ce-COPs)。筛选出电化学信号最强的多面体状Ce-COP为信号探针。通过凝血酶(TB)与TB适体链之间的特异性识别作用，设计了一种简单通用的TB适体传感器。最优实验条件下，该传感器对TB的线性响应范围为1.0 fmol·L-1~1.0 nmol·L-1，检测限为0.94 fmol·L-1。此外，本方案方法与商品人凝血酶(TM) ELISA试剂盒检测结果相近。结果表明，我们构建的TB适体传感器具有良好的灵敏度、特异性、选择性和稳定性。 | |
| 关键词: 铈配合物 传感器 凝血酶 适体 电化学 | |
| 基金项目: 国家自然科学基金青年项目(No.22204010)、重庆市自然科学基金研究项目(No.CSTB2022NSCQ-MSX0967)、重庆市教委科技研究项目(No.KJQN202201631)和重庆第二师范学院儿童日用品检测研究所(No.2022xjyjs12)资助。 | |
| Abstract: In this work, a series of cerium coordination polymers (Ce-COPs) with different morphology and electro- chemical signals were synthesized by a simple hydrothermal method using Ce3+ as the central ion and N,N-dimethyl- formamide as the organic ligand through temperature regulation. The polyhedral Ce-COP with the largest electro- chemical signal was selected as the signal probe. Then, a sensitive thrombin (TB) aptamer sensor was designed through the specific recognition between TB and TB aptamer. Under the optimal experimental conditions, the linear range for TB detection was from 1.0 fmol·L-1 to 1.0 nmol·L-1, and the detection limit was 0.94 fmol·L-1. In addition, our method is similar to the results of commercial human thrombin (TM) ELISA reagent detection. In a word, our biosensor possesses good sensitivity, specificity, selectivity, and stability. | |
| Keywords: cerium coordination polymer sensor thrombin aptamer electrochemistry | |
| 投稿时间：2023-01-03 修订日期：2023-09-17 | |
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| 王存,邹晓川,黄远波,胡静,邓明川,冯霞.基于铈配合物信号探针的凝血酶电化学适体传感器[J].无机化学学报,2023,39(10):1931-1940. | |
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