In vitro antibacterial, antioxidant, in silico molecular docking and ADEMT analysis of chemical constituents from the roots of Acokanthera schimperi and Rhus glutinosa

Acokanthera schimperi is a medicinal plant traditionally used for the treatment of wounds, scabies, and malaria. Rhus glutinosa has been also utilized for the management of ectoparasites and hemorrhoids. Silica gel column chromatography separation of CH 2 Cl 2 /MeOH (1:1) extract root of A. schimperi afforded oleic acid ( 1 ), lupeol ( 2 ), dihydroferulic acid ( 3 ), acovenosigenin A-3-O-α-L-rhamnopyranoside ( 4 ) and sucrose ( 5 ) whereas CH 2 Cl 2 / MeOH (1:1) and MeOH roots extracts of R. glutinosa afforded β-sitosterol ( 6 ), (E)-5-(heptadec-14-en-1-yl)-4,5-dihydroxycyclohex-2-enone ( 7 ), methyl gallate ( 8 ), and gallic acid ( 9 )


Introduction
Infectious diseases have been frequently found to be among the major causes of threat to global health [1].The World Health Organization estimates that infectious diseases account for about 50% of all fatalities in tropical countries [2].The misuse of conventional antimicrobial drugs results in the progress of multiple-drug resistance of pathogenic microorganisms [3].The prolonged bacterial infectious diseases are also important for the emergency of reactive oxygen species (ROS) and are linked with oxidative stress [4].Oxidative stress plays a critical role in the development of various pathological complications such as cardiovascular dysfunction, inflammation, carcinogenesis, drug toxicity, and neurodegenerative diseases [5].Higher plant natural products may be a novel source of antioxidant and antibacterial chemicals with possibly unique mechanisms of action [6,7].Antimicrobials cause harm to pathogens by disrupting the establishment of microbial cell walls, inhibiting the synthesis of microbial proteins and nucleic acids, altering the shape and function of microbial membranes, or blocking metabolic pathways by inhibiting essential enzymes [8].Plant-derived antioxidants may act as free radicals scavengers and combat oxidative stress by keeping a balance between antioxidants and oxidants [9].Antioxidant compounds block the effects of free radicals which have been linked to the pathogenesis of many diseases including atherosclerosis, heart disease, cancer, and Alzheimer's disease [10].The antioxidant potential of the plant product is mainly associated with compounds such as flavonoids and phenolic acids, ascorbic acid, vitamin E, and different carotenoids [11].Recently, researchers have been paying more attention to new antimicrobial and antioxidant compounds isolated from various medicinal plants which might be good sources of novel therapeutic agents [12].
Acokanthera schimperi (Apocynaceae) is a droughtresistant evergreen plant that grows in southern Yemen and East Africa including Ethiopia [13]. A. schimperi has been reported to possess traditionally for the management of wounds, malaria, scabies, and insect repellent [14].In Ethiopia, A. schimperi locally called "Merenz" (in Amharic) and "Kararu" (in Afaan Oromo) have been used for the treatment of headaches, eye disease, skin infection, and snake bites as anti-venom [15].The leaves and bark of the plant have been used for the treatment of skin disorders, inflammatory-related diseases, and malaria [16].In Ethiopia, the grounded bark is mixed with water and taken orally to treat stomachache while its roots are burned on fire and fumigated to cure hepatitis [17].There are limited studies on leaves of A. schimperi that reported the isolations of only a few triterpenoids and acaricidal coumarins from the leaves part of the plant [18,19].A more comprehensive study of compounds isolated from A. schimperi is needed.
Rhus glutinosa (Family Anacardiaceae) is a medicinal plant indigenous to Ethiopia and widely used for the treatment of various ailments [20,21].The dry/fresh stem bark of R. glutinosa is orally given for deficiency of vitamin C [22].In Eriteria, the leaves of R. glutinosa have been used for the management of ectoparasites and scabies [23].In Ethiopia, the dried roots and leaves of R. glutinosa are also used to treat hemorrhoids [24].To the best of our knowledge, even though the plant possesses a wide range of therapeutic values, prior scientific investigations done on the plant are very limited.Herein, we report a comprehensive study on the isolation and characterization of chemical constituents of the roots of A. schimperi and R. glutinosa, and evaluation of their in vitro antibacterial and antioxidant activities and in silico molecular docking and ADMET analysis.

Chemicals and instruments
FT-IR spectra were recorded in the KBr pellet using Thermo Scientific Spectrometer SMART iTR basic in NICOLET iS10 model within a 4000 -400 cm -1 spectral range. 1 H and 13 C NMR spectral data were analyzed on a JNM-ECZ 400 S (400 MHz, JEOL, Ldt.USA) spectrometer.CDCl 3 and CD 3 OD were used as solvents for NMR analysis.TLC silica gel Merck pre-coated aluminum plate (silica gel GF 254 German) was used for TLC analysis, and compounds on TLC were identified with a UV lamp (UV4AC6/2, CBIO, Bioscience, and Technologies.Ldt., China) at 254 and 365 nm and spraying with vanillin/H 2 SO 4 .Column chromatography was used to isolate compounds (silica gel mesh size of 60-120 mm as stationary phase).A double-beam UV-Vis spectrophotometer (P9-UV-Vis, VWR International Ltd, China) was used to measure absorbance at 517 nm for the DPPH assay.Siliga gel (60-120 mesh, LOBA CHEMIE PVT.LTD., India) was used for column chromatography.HPLC grade solvents such as methanol (Alpha Chemika, India) ethyl acetate (Alpha Chemika, India), Chloroform (Alpha Chemika, India), dichloromethane (LOBA CHE-MIE PVT.LTD., India), and petroleum ether (60-80ºC) (LOBA CHEMIE PVT.LTD., India) were used for the isolation of compounds.2,2-diphenyl-1-picrylhydrazyl (DPPH) was used to evaluate the radical scavenging activity of compounds.Ciprofloxacin and ascorbic acid were used as the standard drug for antibacterial and antioxidant assays respectively.

Plant material collection
The roots of A. Schimperi and R. glutinosa were collected in April 2023 from Lalibela Town, North Wollo Zone, Amhara Regional State, Ethiopia, located about 710 km north of the capital, Addis Ababa.The plants were authenticated by Mr. Melaku Wondafrash and a voucher for the specimen BA001 and BA004 was given, respectively, for A. schimperi and R. glutinosa and deposited at the National Herbarium, Department of Biology, Addis Ababa University, Addis Ababa, Ethiopia.

Extraction and isolation of compounds Extraction and isolation of compounds from the roots of A. schimperi
Powdered roots of A. schimperi (600 g) were soaked for 72 h with 4 L of CH 2 Cl 2 /MeOH (1:1), filtered, and concentrated using a rotary evaporator at 40 °C to afford 20 g (4.9%) crude extract.The marc was soaked with methanol (3 L) for 72 h, filtered, and concentrated to afford 6 g (1%).Preliminary TLC profiles of CH 2 Cl 2 /MeOH (1:1) and MeOH extract were examined with the help of a UV cabinet (UV 254 and UV 365 nm lamps) and vanillin/ H 2 SO 4 as a spraying agent.CH 2 Cl 2 /MeOH (1:1) extract (15 g) was adsorbed with an equal amount of silica gel and subjected to chromatographic separation (silica gel 150 g, 60-120 mesh size as stationary phase).Column elution was done by increasing the gradient of EtOAc in petroleum ether to collect 130 fractions (each 100 mL).The TLC profile of each fraction was detected under a UV cabinet (254 and 365 nm) and visualized after spraying with vanillin/H 2 SO 4 .Fractions 8-13 (eluted with 10% EtOAc in petroleum ether) afforded compound (1, 18 mg) as a single spot.Fractions 17-23 (eluted with 10% EtOAc in petroleum ether) showed a similar spot, combined fractions, and re-chromatographed using the increasing gradient of EtOAc in petroleum ether.A total of 36 fractions were collected of which sub-fractions 19-25 (eluted with 10% EtOAc in petroleum ether) showed a single spot after spraying with vanillin/H 2 SO 4 to give compound 2 (23 mg).Fractions 32-40 (eluted with 30% EtOAc in petroleum ether) were combined and rechromatographed using gradient increasing of EtOAc in petroleum ether.A total of 35 fractions were collected of which sub fractions 21-25 (30% EtOAc in petroleum ether) showed a single spot under UV 254 lamp to afford compound 3 (26 mg).Fractions 76-79 formed a white precipitate and showed a single spot after spraying with vanillin/H 2 SO 4 to give compound 4 (57 mg).Fractions 93-97 (eluted with 10% MeOH in EtOAc) were mixed and purified with silica gel column chromatography with increasing gradient of MeOH in EtOAc to obtain compound 5 (40 mg).

Extraction and isolation of compounds from the roots of R. glutinosa
Powdered roots of R. glutinosa (600 g) were soaked with 4 L of CH 2 Cl 2 /MeOH (1:1) for 72 h, filtered, and concentrated using a rotary evaporator at 40 °C to afford 15 g (2.5%) crude extract.The marc was soaked with methanol (3 L) for 72 h, filtered, and concentrated to afford 7 g (1.16%) crude extract.Preliminary TLC profiles of both crude extracts showed similar spots under a UV lamp and hence combined.The combined crude extract (16 g) was adsorbed with an equal amount of silica gel and subjected to silica gel column chromatographic separation with increasing gradient of EtOAc in petroleum ether as eluent to afford 150 fractions (100 mL each).The TLC profile of each fraction was analyzed under UV cabinet at 254 and 365 nm wave length.Fractions 11-16 (eluted in 10% EtOAc in Petroleum ether) showed a single spot to afford compound 6 (23 mg).Fractions 26-33 (eluted in 20% EtOAc in petroleum ether) were combined and rechromatographed using an increasing gradient of EtOAc in petroleum ether.A total of 32 fractions were collected of which sub-fractions 17-22 showed a single spot under the UV 254 lamp to afford compound 7 (17 mg).Fractions 53-62 (eluted with 50% EtOAc in petroleum ether) were combined and purified by silica gel column chromatography using gradient increasing of EtOAc in petroleum ether to collect a total of 62 fractions (10 mL each).Subfractions 28-34 (eluted with 50% EtOAc in petroleum ether) and sub-fractions 49-53 (eluted with 60% EtOAc in petroleum ether) showed a single spot under the UV 254 lamp to afford compounds 8 (40 mg) and 9 (70 mg), respectively.

Antibacterial activity
The antibacterial potential of the crude extracts and isolated compounds were evaluated using Mueller Hinton agar medium following the standard procedure reported in the literature [25] against Gram-negative bacteria (E. coli and P. aeruginosa) and Gram-positive bacteria (S. aureus and S. pyogenes).In brief, a few colonies (3-5) of bacterial strains with similar morphologies were properly introduced into a liquid medium (saline solution) using a sterilized inoculating loop.The turbidity of the suspension was adjusted to 0.5 McFarland barium sulfate standards (10 8 CFU/mL).The experiment was carried out at two-fold serial solutions of 200, 100, and 50 mg/ mL of CH 2 Cl 2 /MeOH (1:1) and MeOH crude extracts, and 5, 2.5, and 1.25 mg/mL of the isolated compounds using DMSO as the diluting agent.Bacterial suspensions were applied onto a petri plate containing MHA. Whatman No 1 paper discs (6 mm) were applied on the surface of the inoculated MHA and about 20 µl each test sample was applied onto the disks.The samples were tested in triplicates and expressed as mean ± SD.Ciprofloxacin (30 µg) was used as the reference drug.

Radical scavenging activity
The radical scavenging activities of crude extracts of roots of A. schimperi and R glutinosa and isolated compounds were evaluated following DPPH assay as described by Sasikumar et al., [26] using ascorbic acid as standard.Two-fold serial dilution solutions of 1000, 500, 250, 125, and 62.5 µg/mL were prepared from 2 mg/mL stock solutions.Then, 4 mL of DPPH (4 mg/100 mL in methanol) was added to 1 mL of each serially prepared sample and kept in the dark for 30 min.The absorbance was measured using a UV-Vis spectrophotometer at 517 nm.The percentage of inhibition of DPPH radical was calculated as; where A control is the absorbance of DPPH, and A sample is the absorbance of the sample with DPPH solution.The inhibitory concentration, the concentration needed to scavenge 50% of DPPH radical (IC 50 ) was calculated from the Prism dose-response curve (GraphPad, Prism version 4.0 for Windows, GraphPad Software, San Diego, CA, USA) obtained by plotting the percentage of inhibition versus the concentrations [27].

Molecular docking analysis
The present study aimed to support the in vitro study via in silico molecular docking analysis by predicting the mode of binding orientation and affinity of the isolated compounds towards the antibacterial and antioxidant target proteins.Based on the in vitro analysis, compounds with promising antibacterial activity (2, 3, 8, and 9) were docked against proteins such as DNA gyrase B of E. coli (PDB ID: 4F86), pyruvate kinase of S. aureus (PDB ID: 3T07), and S. pyogenes 10,782 streptopain (PDB ID: 6UKD) receptors.The selection of antibacterial target protein towards the corresponding docked compound was chosen following the findings obtained in vitro results.The promising DPPH radical scavenging activity of compounds noted by 3, 8, and 9 observed in the experimental analysis also inspired us to predict the binding pattern and affinity of these compounds towards human myeloperoxidase (PDB ID: 1DNU), an important antioxidant target protein.

Protein and ligand preparation
The 3D crystal structures of the selected target proteins (PDB ID: 4F86, PDB ID: 3T07, PDB ID: 6UKD, and PDB ID:1DNU) were downloaded from the RCSB Protein Databank (RCSB PDB: Homepage) and saved in the PDB format.The protein preparation was done using AutoDock 4.2.6 (MGL tools 1.5.6)following the standard protocol [28] by removing the co-crystallized ligand, water molecules, and co-factors.Polar hydrogens and Kollman charges were added to the protein and a pdbqt format file was generated using the AutoDockTools 1.5.6 software.The chemical structures of the studied compounds (2, 3, 8, and 9) were drawn using ChemOffice tool (Chem Draw 16.0) assigned with proper 2D orientation, and the energy of each molecule was minimized using ChemBio3D.The energy-minimized ligand molecules were saved in pdbqt format using the AutoDock Vina tool to carry out the docking simulation.

Docking simulations
The graphical user interface program was used to set the grid box for docking simulations.The grid was set so that it surrounds the region of interest in the macromolecule.Accordingly, the 3D sizes of the structure-based design (SBD) site sphere of each protein were retrieved as follows; (PDB ID: 4F86 (1.979, 8.744, 62.387), PDB ID: 3T07 (0.013, 0.147, -0.003) PDB ID: 6UKD (-25.515,-13.743, 13.171) and PDB ID:1DNU (27.535, 2.689, 13.523) Å for x, y and z dimensions, respectively.The AutoDock Vina searched for the best-docked conformation between isolated compounds and target proteins.Nine different conformations were generated for each ligand in the docking process using the Lamarckian genetic algorithm (LGA) program.The conformations with the most favorable (least) binding free energy were selected for analyzing the interactions between the target receptor and ligands by Discovery Studio visualizer and PyMOL.Finally, the ligand interactions, hydrogen bonds, residual amino acid interactions, 2D and 3D structural orientations, and image preparations were run using the Biovia Discovery Studio Visualizer 2021 [29,30].

In-silico pharmacokinetics analysis
The pharmacokinetic properties of the studied compounds (2, 3, 8, 9) were assessed to estimate their potential as drug candidates.This estimation was carried out based on the standard protocol adopted by the Lipinski rule [31].Pro Tox II was employed to predict the toxicities, toxicological endpoints, and LD 50 of the isolated compounds [32].

Characterization of compounds from the root of A. schimperi
CH 2 Cl 2 /MeOH (1:1) roots extract of A. schimperi afforded five compounds (1-5) (Fig. 1). 1 H NMR spectrum (SI-Figure S1A, SI-Table S1) of compound 1 ranged from δ 0.88 to 5.33.The signals displayed at δ 5.33 (m) and 2.34 (2 H, t) were attributed, respectively, to olefinic and methylene protons α-to an acyl group.The signals observed at δ 1.63 (2 H, m) and 2.02 (4 H, m) were recognized for methylene (H-3) and allylic methylene protons (H-8 and H-9), respectively.The signals displayed at δ 1.25 (24 H, 12 x CH 2 , brs) and δ 0.88 (3 H, t) were assigned to the long-chain methylene protons and terminal methyl protons, respectively [33].The 13 C NMR and DEPT-135 spectra (SI-Figure S1B&C, SI-Table S1) revealed a total of 18 well-resolved carbons.The signal observed at δ 180.4 was an indicative of the presence of a carboxylic acid.The peaks appeared at δ 129.9 and 129.6 were recognized for olefinic methine carbons.The presence of 14 methylene carbons ranged from δ 22.7 to 34.0, pointing downward in the DEPT-135 spectrum, and one terminal methyl carbon displayed at δ 14.1 indicated the presence of unsaturated 18-carbon fatty acid.The above spectra data and comparing it with the literature report indicated that compound 1 is identified as oleic acid [34].
The peaks on the 1 H NMR spectrum (SI-Figure S2 A, SI-Table S2) of compound 2 range from δ 0.74 to 4.67.S2) revealed a total of 30 well-resolved carbons.The 13 C NMR spectrum suggests the presence of seven methyls, eleven methylenes, six methines, and six quaternary carbons.The appearance of olefinic peaks displayed at δ 109.4 (C-29) and 151.0 (C-20) and oxymethine at δ 79.1 (C-3) confirmed that compound 2 was a lupane-type triterpenoid.Based on the above spectral information and comparison with literature reports, compound 2 was recognized as lupeol which has been reported to possess antioxidant, antimicrobial, antitumor, and anti-carcinogenic activities [36].
The FT-IR spectrum (SI-Figure S8A) of compound 8 showed a broad band observed at 3280, cm − 1 representing the O-H stretching vibration, and the intense band that appeared at 1701 cm − 1 was assignable to C = O strtching of an ester group.The bands displayed at 2931 was attributed to the C-H stretching vibration of methyl group.The presence of C = C stretching vibration of aromatic carbons was observed at 1608, and 1450 cm − 1 .The band displayed at 1009 cm -1 was recognized for C-O stretching vibration. 1H NMR spectrum of (SI-Figure S8B) showed the presence of aromatic protons displayed at δ 6.90 (2H, s, H-2/6) and methoxy proton at δ 3.70 (3 H, s).The broad singlet peak observed at δ 9.16 was recognized for the protons belonging to the hydroxyl group.The 13 C NMR and DEPT-135 spectra (SI-Figure S8C & D) revealed a total of six well-resolved carbons.The presence of a symmetry sp 2 methine carbons (C-2/ C-6) was displayed at δ 109.0.The signals observed at δ 119.8 (C-1), 138.9 (C-4), and 146.1 (C-3/C-5) were recognized to be sp 2 quaternary carbons.The presence of a methoxy carbon resonated at δ 52.0 and α-β unsaturated carbonyl δ 166.9 was indicative of the presence of an ester group.Based on the above combined spectral data and comparing it with the literature report, compound 8 was identified as methyl gallate [45].
The FT-IR spectrum (SI-Figure S9A) of compound 9 showed the presence of O-H and C = O stretching vibration at 3345, and 1680 cm − 1 , respectively.The vibrational stretching of C = C of aromatic carbons were observed at 1600, 1550, and 1446 cm − 1 The bands observed at 1320, and 1052 cm − 1 were assigned to -C-O-stretching vibrations. 1 H NMR spectrum (SI-Figure S9B) showed the presence of singlet aromatic proton integrated for two protons observed at δ 6.87 (2H, s, H-2/6).The peaks observed at δ 8.80, and 9.15 were assigned to the protons of the hydroxyl group.The signal displayed at δ 11.80 was attributed to the proton belonging to the carboxyl group.The 13 C NMR spectrum supported its DEPT-135 spectrum (SI-Figure S9C&D) showed a total of five wellresolved carbons.The presence of sp 2 methine carbon (C-2/C-6) was observed at δ 109.0.The signals observed at δ 119.8 (C-1), 138.9 (C-4), and 146.1 (C-3/C-5) were recognized as sp 2 quaternary carbons.The peak observed at δ 168.0 was attributed to the presence α-β unsaturated carboxylic acid.The above spectroscopic information and comparison with the literature reported indicated that compound 9 is identical to gallic acid [45].

Antibacterial activity
The antibacterial activity of the CH 2 Cl 2 /MeOH and MeOH root extracts of A. schimperi and R. glutinosa and isolated compounds (2, 3, 4, 6, 7, 8, and 9) were evaluated against E. coli, P. aeruginosa, S. aureus, and S. pyogenes using disc diffusion assay.The CH 2 Cl 2 /MeOH (1:1) and MeOH extracts of the root of A. schimperi and R. glutinosa exhibited low to promising antibacterial activity following dose-dependent approaches against the tested pathogens (Table 1).The CH 2 Cl 2 /MeOH (1:1) and MeOH extract of the root of A. schimperi showed significant antibacterial activity against E.coli and S. aureus with respective ZI of 16.0 ± 0 and 15.6 ± 0.47 mm at 200 mg/mL, compared to ciprofloxacin (27.0 ± 0 to 31 ± 0.0 mm at 30 µg/disc).MeOH extract also exhibited modest antibacterial activity against S. pyogenes and S. aureus with ZI of 13.0 ± 0.0 and 14.3 ± 0.8 mm, respectively.A similar study carried out by Taye et al., (2011) indicated that MeOH extract leaves of A. schimperi showed moderate antibacterial activity with ZI of 14 mm against against S. pyogenes and S. aureus 500 mg/ mL [46].According to Tadeg et al., (2005) investigation, the MeOH extract of leaves of A. schimperi demonstrated potent antibacterial activity against S. aureus and P. aeruginosa with inhibition of 24 ± 0.5 and 17.0 ± 0.0 mm at 100 mg/mL [47].CH 2 Cl 2 /MeOH extract root of A. schimperi also demonstrated modest activity against E. coli and S. pyogenes with ZI of 13.3 ± 0.8 and 12.6 ± 0.47 mm, respectively.A related investigation conducted by Kenubih et al., (2021) demonstrated that the CH 2 Cl 2 extract leaves of A. schimepri exhibited significant antibacterial activity against E. coli, P. aeruginosa and S. aureus with ZI of 16.67, 15.67, and 15.0 mm, respectively [14].The CH 2 Cl 2 /MeOH (1:1) and MeOH extracts root of R. glutinosa ZI displayed significant antibacterial effects against S. aureus with 17.3 ± 0.8 and 18.0 ± 0.0 mg/mL, respectively, at 200 mg/mL compared to ciprofloxacin (31 ± 0.0 at 30 µg/disc) (Table 1).MeOH extract also showed significant activity against E. coli and S. pyogenes with ZI of 16.3 ± 0.8 and 16.0 ± 0.0 mm, respectively.CH 2 Cl 2 /MeOH (1:1) extract displayed a significant antibacterial action against S. pyogenes and E. coli with ZI of 15.6 ± 0.47 and 15.0 ± 0.0 mm, respectively.The present finding revealed that both plant extracts had significant antibacterial activity against all bacteria tested with varying degrees of activity.The differences in the antibacterial potential of the CH 2 Cl 2 /MeOH (1:1) and MeOH extracts against the tested bacteria could be attributed to the differences in the chemical constituents and the concentration of the secondary metabolites present in the corresponding crude extracts [14].
The antibacterial activity of the isolated compounds (2, 3, 4, 6, 7, 8, and 9) demonstrated modest to promising activity towards the tested pathogens (Table 2).Lupeol (2) displayed the highest antibacterial activity against E.coli and S. aureus with ZI of 16.6 and 17.3 mm respectively, compared to ciprofloxacin (27.0-31.0 ± 0.0 mm).The finding was in good agreement with the literature report demonstrated that lupeol (2) exhibited antibacterial activity against E. coli and S. aureus with ZI of 19 and 20 mm respectively at 3 mg/mL [48].Lupeol (2) also showed modest antibacterial activity P. aeruginosa and S. pyogenes with ZI of 12.3 and 11.6 mm respectively.Musa et al., (2024) investigated on evaluation of the antibacterial potency of lupeol (2) using the broth dilution method and the authors found that the lupeol (2) exhibited antibacterial activity against E.coli and S. aureus with MIC values of 2.5 and 5 mg/mL respectively [49].Dihydroferulic acid (3) displayed the highest antibacterial activity against S. pyogenes and E. coli with ZI of 14.6 and 12 mm respectively.Compound 4 demonstrated significant antibacterial activity S. pyogenes, S. aureus, and P. aeruginosa with ZI of 15.3, 13.6, and 14.6 mm respectively (Table 2).The antibacterial activity outcome of the studied compounds from the root of A. schimperi indicated that   2) may be associated with its nature of lipophilicity and it readily penetrates the cell membrane and exerts its biological effect [50].β-sitosterol (6) showed modest antibacterial activity against S. aureus with an inhibition zone of 11.0 mm, compared to ciprofloxacin (31 ± 0.0 mm).A similar study investigated by Sen et al., (2012) indicated that β-sitosterol ( 6) exhibited moderate antibacterial activity against E. coli and S. aureus with ZI of 13 and 14 mm respectively [51].According to Melaku et al, (2022) investigation, βsitosterol (6) had antibacterial activity against S. aureus, K. pneumoniae, and E. coli with ZI of 12 mm, 11 mm, and 10 mm respectively [52].Compound 7 exhibited potent activity against E. coli and S. pyogenes with ZI of 15.6 and 16 mm respectively.Compound 7 and methyl gallate (8) also demonstrated significant antibacterial effects against P. aeruginosa with of 14.3 and 15.6 mm respectively compared to ciprofloxacin (ZI of 29.0 ± 0.0).Methyl gallate (8) showed potent antibacterial action against S. aureus and E. coli ZI of 18.6 and 16.3 mm respectively.Gallic acid (9) noted the best antibacterial activity S. pyogenes and S. aureus with ZI of 16 and 17.6 mm respectively (Table 2).A related study investigated by Al-Zahrani (2019) revealed that methyl gallate (8) and gallic acid (9) exhibited antibacterial activity against methicillin-resistant and susceptible methicillin S. aureus strains with MIC values of ranges 3.5 to 25 µg/mL [53].A study investigated by Pinho and his coworkers (2014) showed that gallic acid presented potent antibacterial activity against S. epidermidis, S. aureus, and K. pneumonia with MIC values of 0.0098, 0.0195, 0.00975 mg/mL, and MBC values of 0.0039, 0.039 and 0.0039 mg/mL respectively [54].Phenolic compounds such as methyl gallate (8) and gallic acid (9) disrupt the bacterial cell wall by hyperacidification of the plasma membrane via proton donation and acidification of the intracellular cytosolic which inhibits the enzyme H + -ATPase, which is required for the synthesis of ATP [55].
The present study demonstrated that gram-positive bacteria were more susceptible than gram-negative bacteria.The susceptibility of gram-positive bacteria could be associated with the structure of the cell membrane of the bacteria, that gram-positive bacteria lack the outer membrane and have a thick layer of peptidoglycan that surrounds the plasma membrane to protect bacteria from the harsh environment where they livein [56].The present finding revealed that the crude extracts and isolated compound of roots of R. glutinosa demonstrated moderate to promising antibacterial activities against the tested pathogens proving the therapeutic values of the plant recorded by traditional medicinal healers.

Radical scavenging activity
Plants contain abundant antioxidants which provide to defense mechanism against free radicals-associated disorders.Natural antioxidants have the power to stop chain reactions and prevent oxidation processes by eliminating radical intermediates and oxidizing themselves [57].Recently, the radical scavenging and antioxidant capacity of plant natural products have drawn a lot of interest due to their potential to lower the risk of cardiovascular disease and cancer in humans [58].In the present study, the antioxidant activity of CH 2 Cl 2 /MeOH (1:1) and MeOH extracts, and isolated compounds that have enough amount for activity (2, 3, 4, 6, 7, 8, and 9) of the roots of A. schimperi and R. glutinosa was determined following the DPPH assay using compared with ascorbic acid.The dark purple solution of DPPH was turned to yellow when the DPPH radical subsequently received an electron or a hydrogen radical from an antioxidant and the observed color change was measured by UV-Vis spectrophotometry at 517 nm [59].The crude extracts and isolated compounds of (2, 3, 4) of the root of A. schimperi studied compounds demonstrated moderate to strong quenching of DPPH free radicals (Table 3; Fig. 2).CH 2 Cl 2 /MeOH (1:1) and MeOH extracts of the roots of A. schimperi showed modest inhibition of DPPP radical with IC 50 values of 43.0 and 32.89 µg/mL respectively, compared with ascorbic acid (IC 50 value 5.83 µg/Ml).A related study investigated by Alemu et al., (2020) demonstrated that the methanol extract of leaves of A. schimperi exhibited 74.85% DPPH radical inhibition at 200 mg/mL [60].Dihydroferulic acid (3) displayed strong scavenging of DPPH radical with an IC 50 value of 10.66 µg/mL, compared  .The observed promising antioxidant potential of dihydroferulic acid (3) could be associated with the nature of the phenolic compound that could easily donate hydrogen to stabilize the DPPH radicals [63].
The DPPH radical scavenging activity of CH 2 Cl 2 / MeOH and MeOH extract and isolated compounds (6, 7, 8 and 9) of the roots R. glutinosa demonstrated moderate to promising DPPH radical scavenging activity (Fig. 3; Table 4).The CH 2 Cl 2 /MeOH (1:1) and MeOH extract showed strong scavenging of DPPH radical with respective IC 50 values of 20.08 and 13.66 µg/mL respectively.β-sitosterol (6) displayed minimum scavenging of DPPH radical with IC 50 values of 114.5 µg/mL and compound 7 showed medium inhibition of DPPH radical with IC 50 values of 66.86 µg/mL.Methyl gallate (8) and gallic acid (9) demonstrated strong quenching of DDPH radical with respective IC 50 values of 7.48 and 6.08 µg/mL, compared to ascorbic acid (IC 50 value of 5.83 µg/mL).A similar investigation studied by Habtemariam (2011) indicated that gallic acid (9) showed 50% inhibition of DPPH radical at 6.26 ± 0.83 µg/mL [64].According to the  8) exhibited potent scavenging of DPPH radical with an IC 50 value of 1.02 µg/mL [65].Another study studied by Bhadoriya et al., (2012) indicated that gallic acid (8) displayed 91.99 ± 0.59 inhibition of DPPH radical at 50 µg/mL [66].The findings indicated that methyl gallate (8) and gallic acid (9) showed the highest DPPH scavenging activity than the crude extracts and other isolated compounds suggesting that these compounds could be the active antioxidant constituents of the roots of R. glutinosa.The presence of the phenolic hydroxyl group has been comprehensively reported as the main structural descriptor for the antioxidant activity of polyphenols, due to the hydrogen atom ability to scavenge free radicals [67].Gallic acid (9) has been demonstrated as the chief antioxidant component responsible for the efficient antiradical and anticancer properties of several plant extracts [68].The present finding confirms that the crude extracts and isolated compounds of the roots of A. schimperi and R. glutinosa possess antibacterial and antioxidant activity indicating that the chemical constituents of the studied plant cloud have a therapeutic value of pathogenic microorganism and free radical-inducing disorders.

Molecular docking analysis
Most pharmaceuticals or biologically active compounds work by interacting with targets on proteins.Due to many drugs that can target multiple proteins, target identification is crucial in drug development and biomedical studies [69].Molecular docking is an effective method for determining the possible drug candidates by predicting the binding affinity of small molecules to target protein.Docking analysis is used to screen a large database of small molecules to identify those with a high affinity for binding to a particular protein [70].
Drug targets are proteins or enzymes whose activities affect the survival of a disease-causing organism and therefore inhibiting the activity of these enzymes is harmful to the survival of the microorganisms [71].For instance, the S. aureus protein kinase catalyzes the last rate-limiting step of glycolysis, which is essential to the metabolism of carbohydrates.Pharmacological inhibition of PK may cause MRSA to have impaired metabolism so it could be a promising target for novel antibiotics [72].Similarly, the DNA gyrase of E. coli is an important enzyme for bacterial DNA replication and transcription to maintain topology and integrity [73].Therefore, it is essential to use bacterial DNA gyrase as a target for antibacterial drugs [74].On the other hand, streptopain, a cysteine protease secreted by S. pyogenes is an essential enzyme for full host infectivity due to its ability to cleave host proteins (plasminogen, fibrinogen), antimicrobial peptide, and antibodies [75].Streptopain represents a potential virulence factor target, and its inhibition could enhance complement-mediated host defense functions during S. pyogenes infection [76].Human myeloperoxidase (MPO) is an important enzyme found in human neutrophils, whose main role is to maintain defenses against invading pathogens [77].MPO can catalyze the H 2 O 2 -mediated oxidation of chloride to the powerful oxidizing agent hypochlorous acid (HOCl), which leads to the oxidation (degradation) of biomolecules of pathogens in the phagosome.The initial product of MPO-H 2 O 2 -Cl − system is the potent antimicrobial oxidant hypochlorous acid/hypochlorite (HOCl/OCl).However, recent evidence claims that excessive HOCl production can result in oxidative stress, tissue damage, initiation and propagation of acute and chronic vascular inflammatory disease.HOCl can initiate modification reactions targeting lipids, DNA, and proteins through halogenation, nitration, and oxidative crosslinking [78,79].MPO-derived chlorinated compounds are specific markers for inflammation progression, which attracted considerable intrest in the development of therapeutically useful MPO inhibitors and HOCl scavengers.Therefore inhibition of the MPO target is an important mechanism for the treatment of free radical-induced disorders [78].

Drug likeness, ADME, and toxicity profiles of compounds
Drug-likeness is a prediction that evaluates whether a given organic molecule possesses characteristics that make it suitable for use as an orally active medication [31].The drug-likeness and pharmacokinetics properties of the isolated compounds with high-ranking binding energy scores (2, 3, 8, and 9) were assessed using the Swiss-ADME online tool.Swiss ADME is an authenticated free web tool for investigating and estimating the physicochemical parameter, lipophilicity, water solubility, pharmacokinetics, and drug-likeness following the principles of Lipinski's rule-of-five and Veber's rule [81].The Lipinski rule of Five (RO5) principles states that bioactive compounds with a molecular weight of less than 500, logP of less than 5, less than 5 hydrogen bond donors (nHBD), and less than 10 hydrogen bond acceptors (nHBA) are more likely to have good oral bioavailability and be orally active [82].According to the Veber rule a drug-like molecule should have a total polar surface area (TPSA) should be less than 140 Å and the number of rotatable bond values should be less than 10 [83].The present finding revealed that all compounds under investigation (2, 3, 8, and 9) have a molecular weight in the acceptable range (MW ≤ 500) implying they can be readily absorbed in the gastrointestinal tract [84] (SI-Table S12).The nHBA and nHBD for all tested compounds were found to be within Lipinski's limit range.This implies that the compounds are expected to be well absorbed or permeable from the gastrointestinal tract when they are administrated.The TPSA value of the analyzed compounds was recorded in the range from 20.23 to 97.99 Å and is much below the limit of 140 Å, suggesting a high probability of favorable oral bioavailability for drug-like candidates [85] (SI-Table S12).
The number of rotatable bonds is also an important parameter to estimate the drug-likeness potential of a drug candidate and is linked with the bioavailability and flexibility of lead compounds.Hence, all studied compounds fell within the acceptable range (nRB ≤ 10), indicative of their potential permeability and oral bioavailability.Overall, all the analyzed compounds meet the criterion of Lipinski's rule and Veber's rule with 0 violations, suggesting the potential to be orally administered drugs (SI-Table S12).The skin permeability value (Kp, in cm/s) of the analyzed compounds ranges from − 1.90 to -6.84 cm/s suggesting low skin permeability and is within the range of broad-spectrum antibiotic ciprofloxacin (-9.09 cm/s) [86].The lipophilicity (log P) values of all studied compounds ranges from 0.21 to 4.86 fell under the Lipinski rule and suggested optimal lipophilicity of the compound.The Swiss-ADME screening outcome revealed that except lupeol (2), all compounds under investigation were likely to have high gastrointestinal absorption.This implies that compound (3, 8, and 9) compounds have the potential to be absorbed in the gastrointestinal tract upon oral administration [87] (SI-Table S12).The BBB permeability test results indicated that except for compound 3, all compounds under investigation lacked BBB permeability, revealing these compounds were expected to have a high level of CNS safety [88].The ADME estimation results demonstrated that all compounds under investigation are not found to be substrates of permeability glycoprotein (P-gp), implying favorable drug absorption [89].
Cytochrome P450 monooxygenase plays a fundamental part in drug mobilization and elimination in biological systems.Inhibition of isoforms of this enzyme system by drugs could result in poor elimination, resulting in druginduced toxicity.Therefore it is important to note that a drug candidate should have minimal inhibitory effect against various enzyme isoforms [90].The present finding indicated that none of the tested compounds were found to be inhibitors of CYP1A2, CYP2C19, CYP2C9, CYP2C9, and CYP2D6 isozymes.Moreover, except gallic acid (9) none of the analyzed compounds were inhibitors of CYP23A4, suggesting that the compounds would be well metabolized in the liver and readily eliminated from the organism [91].The results of the Pro Tox II toxicity analysis of the studied compounds (2, 3, 8, and 9) found to have hepatotoxicity, mutagenicity, immunotoxicity, and cytotoxicity.Except for gallic acid ( 9), all tested compounds were found to be noncarcinogenic.The obtained toxicity profile of the analyzed compounds suggested that the compounds might be good candidates for drug development.The acute oral toxicity (LD 50 ) predictions indicated that all the tested compounds were categorized into class 4 (300 < LD 50 ≤ 2000), indicating slight toxicity levels (SI-Table S12).
In conclusion, the CH 2 Cl 2 /MeOH (1:1) extract of the roots of A. schimperi afforded five (1-5) compounds.Of which, dihydroferulic acid (3), acovenosigenin A-3-O-α-L-rhamnopyranoside (4), and sucrose (5) were reported here for the first time from this species.CH 2 Cl 2 / MeOH (1:1) and MeOH extract of R. glutinosa afforded four (6)(7)(8)(9), all reported here for the time from this species.The CH 2 Cl 2 /MeOH (1:1) and MeOH crude extracts and isolated compounds of A. schipmeri and R. glutinosa showed modest to promising antibacterial activity against the tested bacteria strains.Among them, lupeol (2) methyl gallate (8) and gallic acid (9) displayed promising activity against E.coli, S. aureus and S. payogens with an inhibition zone ranges of 16.6 ± 0.09 to 18.6 ± 0.08 mm at 5 mg/mL.Dihydroferulic acid (3), methyl gallate (8), and gallic acid (9) also demonstrated promising scavenging of DPPH radical with IC 50 values of 10.66, 7.48, and 6.08 µg/mL, respectively, compared to ascorbic acid (IC 50 value 5.83 µg/mL).The in vitro antibacterial and antioxidant activities of the crude extracts and isolated compounds of A. schimperi and R. glutinosa supported by the considerable minimum binding free energy of the studied compounds towards the respective target proteins prove that the chemical constituents of the studied plants could serve as antimicrobial agents by blocking the essential cellular reactions required for the survival of the bacterial and the treatment of free radical-induced disorders.Further comprehensive in vivo study is needed to explore the plants' full therapeutic potential and confirm the efficacy and safety of crude extracts and isolated compounds.

1 H
NMR of the spectrum (SI-FigureS5A, SI-Table hydrocarbons.The bands displayed at 1464 and 1383.16cm − 1 represent the C-H bending vibration of methyl groups.Weak bands observed at 1650, and 1048 cm − 1 were assigned to C = C and C-O stretching vibration, respectively.The 1 H NMR spectrum (SI-Figure S6B, SI-Table with ascorbic acid (IC 50 value of 5.83 µg/mL).Lupeol (2) and compound (3) also displayed medium inhibition of DPPH radical with respective IC 50 values of 53.97 and 47.54 µg/mL.The observed antioxidant potential of lupeol (2) showed close agreement in published literature indicating that lupeol established scavenging of DPPH radical with an IC 50 value of 44.3 µg/mL [61].Dihydroferulic acid (3) displayed higher antioxidant activity compared to the crude extracts and other compounds (2 and 4) of A. schimperi.A similar study investigated by Deters et al., (2008) demonstrated that dihydroferulic acid (3) exhibited scavenging of DPPH radical with IC 50 values of 19.5 µM[62]

Table 1
Antibacterial activities reported as inhibition zone (mean ± SD, in mm) of CH 2 Cl 2 /MeOH (1:1) and MeOH extract roots of A. schimperi and R. glutinosa