Synthesis, biochemistry, and in silico investigations of isatin-based hydrazone derivatives as monoamine oxidase inhibitors

Ten isatin-based hydrazone derivatives were synthesized using two subseries, IA (isatin + acetophenone) and IB (isatin + benzaldehyde), and evaluated for their monoamine oxidases (MAOs) inhibitory activity. All the compounds showed stronger MAO-A inhibition than MAO-B, and the IB series showed more effective MAO-A inhibitory activity than IA series. Compound IB4 most potently inhibited MAO-A (half maximal inhibitory concentration IC 50 = 0.015 µM), followed by IB3 (IC 50 = 0.019 µM). On the contrary, compound IB3 showed the highest MAO-B inhibition (IC 50 = 0.068 µM), followed by IB4 (IC 50 = 1.87 µM). Compound IB3 and IB4 had low selectivity indices of 3.68 and 8.50, respectively. Structurally, the methyl group of IA series decreased the inhibition of both MAO-A and MAO-B. Among them, IB3 and IB4 (4-Cl and 4-Br in B-ring, respectively) showed higher MAO-A and MAO-B inhibition than the other substitutions. Inhibition constant K i values of IB3 and IB4 for MAO-A were 0.0088 and 0.0063 µM, respectively, and those for MAO-B were 0.048 and 0.060 µM, respectively. IB3 and IB4 were competitive, reversible inhibitors of MAO-A and MAO-B. Molecular docking analysis predicted that IB3 and IB4 formed stable hydrogen bonds between Asn181 and the NH atom of isatin in the ligand-protein complex. Dynamic analysis revealed that IB3 and IB4 are stable with both MAO isoforms. These observations suggest IB3 and IB4 are potent and reversible MAO-A and MAO-B inhibitors and both compounds can be used as therapeutic agents for neurological disorders.


Introduction
A fused diketopyrrole ring and a benzene ring compose the bicyclic heteroaromatic nucleus of isatin [1].It is found naturally in several plant species and some animal tissues and is used as a versatile starting material in synthetic organic chemistry.Isatin demonstrates a wide range of reactivity owing to the presence of both electron-rich (benzene and non-bonding electrons of nitrogen atoms) and electron-deficient sites (diketo part of 2nd and 3rd positions) in its structure.It can undergo oxidation, substitution, spiroannulation, and condensation reactions to produce various derivatives with unique structural frameworks and characteristics [2][3][4].In medicinal chemistry, isatin and its derivatives have attracted considerable attention because of their diverse biological profiles.These molecules exhibit a wide range of pharmacological activities including antiviral, antibacterial, anti-inflammatory, anticancer, and antidepressant effects.Derivatives of isatin have been studied as possible treatment agents for a number of illnesses, including infectious diseases, cancer, and neurological problems [5][6][7][8].
The brain and other peripheral human tissues have a class of enzymes called monoamine oxidases (MAOs) in their outer membranes of the mitochondria [9,10].They play a prominent role in the metabolism of monoamine neurotransmitters, which are signaling molecules that control mood, emotions, and thought processes.MAO has two primary isoforms: MAO-A and MAO-B.Although there was significant overlap, the substrate specificities and tissue distributions of each isoform varied.The main metabolites of the MAO-A isoform are dopamine, norepinephrine, and serotonin.MAO-A is a major player in the breakdown of serotonin and norepinephrine, which is why drugs are used to treat mood disorders, such as anxiety and depression conditions [11][12][13].MAO-B inhibitors are used to treat Parkinson's disease (PD), which is a neurodegenerative disorder accompanying with the loss of dopaminergic neurons owing to their unique function in the breakdown of dopamine [14,15].As specific inhibitors target only one type of MAO enzyme, they provide a tailored therapy with possibly fewer side effects.In contrast, nonselective inhibitors may work better in some circumstances and have wider therapeutic effects.Furthermore, in order to prevent potentially harmful interactions with foods containing tyramine, which can result in hypertensive crises, non-selective MAO inhibitors require stringent dietary restrictions [16,17].Both enzymes of MAO-A and MAO-B were inhibited by isatin and its derivatives.Isatin and its derivatives inhibits MAO-A and MAO-B with reversible type and with K i values of 3 and 15 µM, respectively [18,19].This implies that the dysregulation of neurotransmitter levels is linked to illnesses, including depression, anxiety, and PD, for which isatin may have therapeutic potential.According to our group, the C-5 and C-6 positions have been more thoroughly reviewed than the N-1 and C-3 positions.Following this, our team investigated the C-3 location and discovered that IS7 (IC 50 = 0.082µM), IHC3 (IC 50 = 1.672µM),ISB1 (IC 50 = 0.124µM), and ISFB1 (IC 50 = 0.135µM) (Fig. 1) were all influential against MAO-B [ [20][21][22][23].The chemical and physical properties of acyl hydrazones are typically attributed to their structural framework, which consist of two interconnected nitrogen atoms.Acyl hydrazones are widely employed in the synthesis of new compounds with MAO inhibition [24][25][26].To improve the pharmacological characteristics of MAO inhibition, isatin-based hydrazones containing various aldehydes and ketones were incorporated in the current study.

Synthetic procedure
To create the appropriate isatin hydrazones, a 2.5:1 mixture of hydrazine hydrate and isatin was dissolved in methanol (7.5 mL) and acetic acid (glacial, as the catalyst).A 1:1 combination of the intermediate and alternatively substituted aldehydes and ketones was then mixed in methanol (7.5 mL), with a catalytic amount of the acetic acid (Scheme 1) [21].To finish the reaction, the mixture was placed in a microwave reactor (Monowave 50) for 10-20 min at 100 °C.Hexane and ethyl acetate were mixed in a 1:1 ratio and the progress of the reaction was checked by thin-layer chromatography.After filtering and air-drying, the product was cleaned and recrystallized from methanol.The 1 H and 13 C NMR data were included in Supplementary Information (Figs.S1 ~ S6).

Enzyme assays and kinetics
Enzyme activities of MAO-A and MAO-B activities were analyzed using the continuous assay method described [27,28].For enzyme kinetics, MAO-A and MAO-B activities were analyzed using five substrate concentrations, i.e., in the range of 0.0075-0.12mM of kynuramine and 0.0375-0.6 mM of benzylamine, respectively.The Michaelis constant (K m ) values of MAO-A and MAO-B were determined to be 0.045 and 0.139 mM, respectively, using Lineweaver (LB) plots.

Inhibition studies of MAO-A and MAO-B
In the primary screening, inhibitory activity against MAO-A and MAO-B at 10 µM concentration of ten compounds were evaluated.IC 50 values, i.e., 50% inhibitory concentration were determined using the GraphPad Prism software 5 [29][30][31].The curves were constructed with 3-9 points of duplicated inhibitory data.Selectivity index (SI) was calculated using the IC 50 for MAO-B/IC 50 for MAO-A.The inhibition characteristics of lead compounds IB3 and IB4 against both enzymes were analyzed using LB plots in the presence of the inhibitors at three concentrations, and the inhibition constants (K i ) were determined using a secondary plot derived from the LB plots [27,28].

Reversibility studies
The reversibility of MAO-A or MAO-B inhibition by IB3 or IB4 was tested using the 6-8 kDa DiaEasy™ dialyzer, by comparing the residual activities of the undialyzed (A U ) and dialyzed (A D ) at ~ 2.0 ∼ IC 50 after 30 min preincubation of inhibitor and enzyme, as previously described [29,32].Reference compounds were included for reversible and irreversible inhibitors, and their patterns were decided by comparing A U and A D of the compounds [29,32].

Molecular dynamic simulation
Molecular dynamics (MD) simulations were carried out using Schrödinger LLC's Desmond simulation tool.Initially, the protein-ligand combination was conducted for the Desmond system builder panel, which targets MAO-A and MAO-B, by means of compounds IB3 and IB4.The simulation parameters were 100 ns at 300 K, 1.01325 bar of pressure, and 1000 frames [21][22][23].

In silico cytotoxicity and pharmacokinetics predictions of the compounds
The cytotoxicity and pharmacokinetics predictions of the compounds were analyzed using the ProTox-3.0web tool (https://tox.charite.de/protox3/,accessed on June 5, 2024) for cytotoxicity, blood-brain barrier (BBB) permeability, and cytochrome P450 inhibition.
Regarding the structure-activity relationship, IB series showed higher inhibition of MAO-A and MAO-B than did the IA series.However, IA series showed higher SI values for MAO-A than did the IB series.In IA series, IA5 (4-OH in B-ring) had 2.09-to 29.75-times higher MAO-A inhibition than did the other IA derivatives with substituents of -OH > -Br (IA4) > -F (IA2) > -Cl (IA3) > -H (IA1) at 4-position in B-ring in order.In addition, IA2 (4-F in B-ring) had 1.29-and 4.25-times higher MAO-B inhibition than did the other IA derivatives with substituents of -F > -OH > -Cl > -Br > -H at 4-position in B-ring in order.In IB series, IB4 (4-Br in B-ring) had 3.93-to 11.33-times higher MAO-A inhibition than did the other IB series, with substitutions of -Br > -Cl (IA3) > -F (IA2) > -H (IA1) > -OH (IA5) at 4-position in B-ring in order.Besides, IB3 (4-Cl in B-ring) had 1.87-to 24.94times higher MAO-B inhibition than did the other IB series, with substitutions in order of -Cl > -Br > -F > -H > -OH (Fig. 2; Table 1).Collectively, IB3 and IB4 (4-Cl and

Enzyme kinetics
IB3 and IB4 were selected as the most potent MAO-B and MAO-A inhibitors, respectively.The kinetics of MAO-A in the presence of IB3 or IB4 were analyzed using five concentrations of kynuramine and three concentrations of inhibitor.In the LB plots, lines of IB3 or IB4 were coincided on the Y-axis with an increase in the K m values and observed to be competitive MAO-A inhibitors (Fig. 3A and C).Secondary plots, slope vs. inhibitor concentration, showed that the K i values were 0.0088 ± 0.0015 and 0.0063 ± 0.0012 µM, respectively (Fig. 3B and D).These results suggest that IB3 and IB4 are competitive MAO-A inhibitors.
The kinetics of MAO-B inhibition by IB3 or IB4 were also analyzed using benzylamine as a substrate.In the LB plots, IB3 and IB4 were observed to be competitive inhibitors of MAO-B (Fig. 4A and C), as similar to the MAO-A inhibition.Secondary plots showed that the K i values were 0.048 ± 0.005 and 0.060 ± 0.017 µM, respectively (Fig. 4B and D).These results suggest that IB3 and IB4 are competitive MAO-B inhibitors.

Reversibility studies
Reversibility of the inhibition by the compound was evaluated using the dialysis method.In this study, concentrations of compounds IB3 and IB4 used were ~ 2.0 times of their IC 50 values (0.040 and 0.030 µM for MAO-A, and 0.14 and 0.26 µM for MAO-B, respectively).Recovery types were determined by comparing the A U and A D values.MAO inhibition by IB3 and IB4 recovered from 22.66 to 84.07% and 27.17% and 82.65%, respectively (Fig. 5A).The recovery values of IB3 and IB4 were comparable to those of the reversible inhibitor toloxatone (from 38.01 to 77.97%) and different from those of clorgyline, the irreversible inhibitor (from 27.62 to 27.41%).In addition, MAO-B inhibition by IB3 and IB4 recovered from 30.81 to 85.67% and from 33.36 to 83.64%, respectively (Fig. 5B).The recovery values of IB3 and IB4 were similar to those of the reversible inhibitor safinamide (from 31.76 to 82.51%) and different from those of pargyline, the irreversible inhibitor (from 34.50 to 35.09%).These results indicate that IB3 and IB4 are reversibletype inhibitors of MAO-A and MAO-B, respectively.

Molecular docking
Extensive molecular docking analyses were conducted to gain deeper insights into the binding mechanisms of lead compounds.Interactions were observed between IB3, IB4, IA3, and IA4 along with MAO-B and MAO-A.Docking was confirmed using native ligands.The compounds were listed in Table 2, with docking scores (XP mode) ranging from − 7.16 to -10.85 kcal/mol.Through docking evaluation, the lead molecules IB3 and IB4 received scores of -10.23 and − 9.95 kcal/mol, respectively.Safinamide, on the contrary, had a score of -10.85 kcal/mol.The docking scores indicated an affinity comparable to that of biological activity (IC 50 ).The lead molecules (IB3 and IB4) exhibited binding interactions with the MAO-B-binding pocket via hydrophobic forces (Fig. 6).
The docking scores for MAO-A, IA3, IA4, IB3, IB4, and Harmine in XP mode ranged from − 7.19 kcal/mol to -3.15 kcal/mol (Table 2).All the molecules were aligned in the same manner as the original ligands.Extensive examination of IB3 and IB4 in the active site of MAO-A revealed their presence at various positions, including Tyr69, Leu97, Phe108, Ala111, Ile180, Asn181, Ile207, Phe208, Val210, Cys323, Ile325, Ile335, Leu337, Phe352, and Tyr407 (Fig. 7).By utilizing pi-pi stacking, the interaction between rings A and B of IB3 and IB4 with Tyr407 and Phe208 was established.A comparable interaction was observed for IA3 and IA4 except for IB4 and IB3, in which Asn181 interacted with NH atom of the isatin ring through hydrogen bonding.In general, molecular docking studies suggest that the interaction between the IB series of molecules and the MAO-A pocket is more stable when hydrogen bonding occurs with the NH atom.

Molecular dynamic simulation
The binding mechanisms of IB3 and IB4 in the inhibitor-binding cavities (IBC) of MAO-A and MAO-B were monitored using MD simulations.The protein C-alpha and ligand remained within an acceptable range throughout the entire simulation duration of 100 ns, as determined by root mean square deviation (RMSD) analysis.The RMSD for the MAO-A protein is within the range The RMSF for each amino acid residue in the protein was determined by the simulation in order to evaluate the system's flexibility.High RMSF values indicate areas with flexibility, whereas low values suggest rigidity of the amino acids.The residues of MAO-A and -B exhibited significant fluctuations (Fig. 8).There were 17 interactions between IB4 and 13 interactions between IB3 and the amino acids of MAO-A.Nearly identical interactions were observed, except for a few specific residues.For MAO-B, there were 17 and 18 interactions for IB3 and IB4, respectively.Nearly identical interactions were observed, except for Tyr60, Pro102, Pro104, Trp119, and Leu328 (Table 3).There were similarities in the manner in which the ligand interacts in 2-D during docking and in the subsequent simulation, suggesting that the ligand might have the ability to inhibit MAO in a non-specific manner.

In silico cytotoxicity and pharmacokinetics predictions
The cytotoxicity and pahrmacokinetics of IB3 and IB4 using ProTox-3.0web tool showed these two compounds had non-toxic and BBB permeability (Table 4).In addition, these compounds showed CYP450 inhibition including the CYP1A2 (Table 4).These results suggest that compounds IB3 and IB4 can be used as therapeutic drugs, but careful consideration of the dosage is required.
Ultimately, the synthesis of isatin-based hydrazone derivatives from two sub-series, IA and IB, was successfully performed, and their effects on MAOs were evaluated.Both series showed a higher level of prominence for MAO-A than MAO-B.IB3 and IB4 are reversible and competitive inhibitors against MAO-B (IC 50 = 0.068 and 0.127 µM, respectively) with low selectivity (SI = 3.68 and 8.50, respectively).Furthermore, the stability of the complex was determined through docking analysis of MAO-A and MAO-B with IB3 and IB4.Stable hydrogen bonding between Asn181 and the NH atom of isatin forms a ligand-protein complex, similar to the work of a pharmacologist.It is possible to conclude from dynamic analysis that IB3 and IB4 are stable when both MAO isoforms are present.In addition, IB3 and IB4 were predicted to be non-toxic and to have BBB permeability

4 -
Br in B-ring, respectively) showed greater MAO-A and MAO-B inhibition than did the other derivatives.These results suggested that IB3 and IB4 are potent inhibitors of MAO-A and MAO-B.

Fig. 2
Fig. 2 Structure-activity relationship (SAR) of IA and IB series

Fig. 3
Fig. 3 LB plots for MAO-A inhibition by IB3 and IB4 (A and C, respectively), and their secondary plots (B and D, respectively) of the slopes vs. inhibitor concentrations.The experiments were analyzed at five concentrations of kynuramine as a substrate and at three inhibitor concentrations

Fig. 4
Fig. 4 LB plots for MAO-B inhibition by IB3 and IB4 (A and C, respectively), and their secondary plots (B and D, respectively).Benzylamine was used as a substrate

Fig. 5
Fig. 5 Recovery of MAO-A (A) and MAO-B (B) inhibitions by IB3 and IB4 using the dialysis experiments.The concentrations of IB3 and IB4 used were 0.040 and 0.030 μM for MAO-A, respectively; 0.14 and 0.26 μM for MAO-B, respectively.After 30 min preincubation, the mixtures were dialyzed for 6 h with twice of buffer change

Table 1
Inhibitions of MAO-A and MAO-B by the 10 compounds of IA and IB series a a Results are the means ± standard errors of duplicate or triplicate experiments b Selectivity index (SI) values are expressed for MAO-B compared to MAO-A

Table 2
Docking scores of MAO-A and MAO-B with IA3, IA4, IB3, and IB4, and their native ligands