Knowledge, attitudes, and readiness about critical antimicrobial resistant organisms among healthcare workers at colonial war memorial hospital in Fiji: a pre and post intervention study

Background Gram-negative bacteria resistant to carbapenems are also known as critical antimicrobial resistant organisms. Their emergence at Colonial War Memorial Hospital (CWMH), the largest hospital in Fiji, is a major clinical concern. This study was conducted to determine the knowledge, attitudes, and readiness of healthcare workers (HCW) at CWMH regarding management of patients with infections caused by critical antimicrobial resistant organisms. Methods A questionnaire was designed using a Likert scale to assess knowledge, attitudes, and readiness. Two cross-sectional studies were conducted, before and after the implementation of targeted educational activities which were informed by the pre-intervention study findings. Results A total of 393 and 420 HCW participated in the pre- and post-intervention studies, respectively. The majority of respondents were female (77.3%) and 18–34 years of age (67%). HCW professional roles included nurses (56.3%), doctors (31.6%), and laboratory personnel (12.2%). In the post-intervention study, significantly more HCW reported having received infection prevention and control (IPC) and antimicrobial resistance education and training (26.8% in pre to 45.5% in post intervention, p < 0.001). The majority of nurses and doctors (> 85% to ≥ 95%) were aware of how AMR organisms spread in healthcare settings and knew the IPC measures to prevent transmission of AMR infections including hand hygiene, standard and transmission-based precautions. Attitudes towards AMR were positive, with 84.2% pre intervention and 84.8% of HCW post intervention expressing their willingness to change their work environment to assist with AMR prevention. Perceived readiness to address the problem showed mixed results. Improvements in laboratory AMR surveillance data availability were noted (29.4–52.4%, p < 0001). Modest improvement in the hospital’s capacity for outbreak response (44–51.9%, p = 0.01), and treatment of AMR infections (38.9–44.4%, p = 0.01) was reported. Conclusions Our data revealed high levels of staff awareness and knowledge about AMR and IPC. However, readiness for outbreak response and treatment of critical AMR infections requires more attention. Improving AMR prevention and containment in CWMH will likely require sustained and multisectoral interventions with strong administrative commitment. Supplementary Information The online version contains supplementary material available at 10.1186/s13756-024-01439-9.


Background
The emergence and spread of antimicrobial resistance (AMR) is a major public health threat globally [1].In 2017, the World Health Organization (WHO) published the priority list of multidrug-resistant organisms (MDRO) for research and urgent development of new antimicrobials [2].The critical AMR priority list includes Acinetobacter, Pseudomonas and various Enterobacterales resistant to carbapenems and other last-line antimicrobials [3,4].Infections with critical AMR organisms in resource-limited settings are a major concern as there is inadequate or limited human, financial and infrastructure capacity to detect infections, limited access and high cost of antimicrobials, and inadequate capacity to implement measures to prevent and control them in healthcare settings [5,6].While AMR can occur naturally, some of the main factors that accelerate the emergence of AMR in healthcare facilities are misuse or overuse of antimicrobials and poor infection prevention and control (IPC) practices [7,8].A lack of knowledge about AMR can be a contributing factor to inappropriate use or overuse of antimicrobials [9].Multimodal IPC measures are effective to prevent the transmission of critical AMR infections in healthcare settings [10].Knowledge of healthcare workers (HCW) about these measures and their consistent application during patient care is of paramount importance.Knowledge, attitudes, and practice studies in low-and middle-income countries have previously showed major gaps in knowledge and practices related to AMR and IPC and highlighted the need for more standardized training to raise awareness and improve attitudes and practices [11][12][13][14].
Fiji is an island nation in the South Pacific with a population of 884,887 in 2017 [15].Recent studies from Fiji's main hospital, Colonial War Memorial Hospital (CWMH) revealed the substantial burden of organisms with critical AMR including carbapenem-resistant Acinetobacter baumannii and Enterobacterales [16,17].In addition, healthcare-associated infections (HAI) and outbreaks caused by MDRO have previously been reported in CWMH [18][19][20][21].Outbreak investigations identified poor IPC practices and shortages of relevant consumables as the main barriers to IPC compliance [19,20].Fiji launched new national IPC guidelines in 2022.Despite managing a high burden of AMR and the recurrent outbreaks of HAI with MDRO, the HCW's knowledge, attitudes, and perceptions of their overall readiness for the diagnosis, prevention, and control of critical AMR organisms has not been investigated.The uptake and usage of the current IPC guidelines is not known.
As the threat of AMR continues to expand in Fiji, more work is required to support the health system to better detect AMR organisms and implement prompt IPC interventions.Preparing Fiji for Organisms with Critical Antimicrobial Resistance is a project supported by the Australian Government's Medical Research Future Fund.This project was jointly implemented by the Fiji Ministry of Health and Medical Services and University of Melbourne between May 2022 through December 2023.As part of this project, two cross-sectional studies (pre-and post-intervention of a capacity building program) were conducted to determine the knowledge, attitudes, and readiness of HCW in CWMH about critical AMR and IPC and to assess the changes associated with the introduction of project interventions.

Study site
CWMH is the largest referral hospital in Fiji with 500 beds and over 26,500 admissions in 2015 [22].
There are a total of 1,879 staff of which, 900 are nurses, 200 doctors and 91 laboratory personnel in 2021 (Unpublished-CWMH statistics).The hospital has five IPC officers and a functional IPC committee.The IPC team works in collaboration with all departments to implement IPC activities and monitor compliance to recommended practices.There is antimicrobial stewardship (AMS) team comprised of consultant physicians, pharmacists and laboratory scientists which conducts weekly AMS rounds in the ICU, provide advice on antimicrobial use and review prescriptions of restricted antimicrobials.The hospital has a microbiology laboratory.Culture and antimicrobial susceptibility testing are performed using approved protocols.

Pre-and post-intervention studies
Two cross-sectional studies were conducted.The preintervention study was conducted in July and August 2022 before the commencement of project activities.Twelve months later, after implementation of project activities, a post-intervention study was conducted in September and October 2023.
The study questionnaire was developed through review of existing literature [23][24][25][26] and questions were appraised by local and international investigators.The questionnaire was piloted with a small group of HCW (n = 11) to assess the validity and reliability.The final questionnaire was divided into three parts.The first part included demographic and professional information (age group, gender, year of service, position, departments, and IPC training).Two additional questions were added in the post-intervention study to gather data on participation in the baseline study and participation in any educational activities regarding IPC and critical AMR in the past 12 months.The questions in part two and three included knowledge, attitudes and readiness questions which required participants to select one answer from a five-point Likert scale.There were 16 knowledge and 10 attitude questions which addressed topics such as AMR surveillance, transmission, prevention, laboratory detection, communication, and IPC management.There were five readiness questions which focused on staff perceptions of the local capacity to detect and respond to outbreaks, surveillance and resources available for AMR case management.The study was promoted to doctors, nurses, and laboratory personnel in person and at departmental meetings.Participation was voluntary and written informed consent was obtained from all respondents.
The sample size calculation was based on estimated baseline knowledge rate of 50%, estimated dropout or incomplete questionnaires rate of 15%, and 10% nonresponse rate, so the required sample size was 390 participants for each of the pre-and post-intervention groups.

Interventions
The project was implemented in CWMH from May 2022 through December 2023.Between May and July 2023, the team conducted baseline IPC and laboratory capacity assessments as well as the pre-intervention survey.1).Audit tools were developed to monitor compliance with IPC recommendations.The IPC team conducted regular contact precautions audits to assess compliance and gave feedback to HCW. (Additional file 1 Table 2).Systematic recording of CRO cases commenced and a line list was regularly updated and shared with treating medical and IPC team.The project also supported CRO outbreak detection and investigation.On site and remote mentorship and regular meetings were held with microbiologists and infection control specialists in Melbourne, Australia.

Data analysis
Data were entered into Microsoft Excel (version 16.79.2) and analysed using R package (version: 4.3.2).Descriptive statistics was used to analyse the socio-demographic, knowledge, attitudes, and readiness data.All categorical variables were presented in proportion and percentages.The Chi-squared test was used to compare the pre-and post-intervention knowledge, attitudes, and readiness.A p-value < 0.05 was considered as statistically significant.

Ethics
The study received ethics approval from Fiji National Human Research Ethics Committee (ID number 11/2022) and Human Ethics Advisory Group of The University of Melbourne (ID number 2022-23602-30481-7).

Results
A total of 816 HCW completed the study questionnaires with the overall response rate of 96% (395/410) of those offered the questionnaire in the pre-intervention period and 84.7% (421/497) in the post-intervention study.
Three respondents were excluded due to incomplete responses and 393 and 420 respondents in the pre-and post-intervention studies were included in the analysis, respectively.

Sociodemographic and professional characteristics
In both studies, around two third of respondents were aged 18-34 years (67%), with a female majority (77.3%) (Table 1).Overall, 61% of HCW had over 5 years of work experience.The HCW professional roles included nurses (56.3%), doctors (31.6%), and laboratory personnel (12.2%).Respondents represented various departments including internal medicine (18.2%), surgery (17.9%), paediatrics (15.3%), obstetrics and gynecology (14%).Laboratory departments included hematology (23%), microbiology (22%), serology (13%), and biochemistry (12%).15% (63/420) of respondents participated in both studies.The pre-and post-intervention study respondents were comparable in terms of age, sex, and professional category.However, work experience significantly differed (Table 1).The pre-intervention respondents had more senior staff, 34.6% with > 10 years of work experience compared to 27.4% in post-intervention study, p = 0.02.The shift was mainly among nurses where significantly more nurses with ≤ 1 year of service took part in the postintervention (11.6%) compared to pre-intervention study (3.3%), p = 0.003.While the proportion of nurses with > 10 years of experience declined from 38.2% in the preintervention to 29.6% in post-intervention study (Additional file 1 Table 3).The proportion of surveyed HCW who reported that they had received IPC training or education increased from 26.8%, in pre-intervention to 45.5% in the postintervention study, p < 0.001 (Table 1).Nearly half (45.9%) of the post-intervention respondents reported receiving information on AMR during the 12 month period of project implementation.In both studies, almost all respondents (> 97%) acknowledged the need for more education and information on critical AMR.(Table 2a)

Knowledge
Most HCW across all categories were aware that critical AMR organisms had been identified in CWMH (80.1% pre-and 93.5%, post-intervention p < 0.001, respectively) (Table 2a).The majority of nurses and doctors Among laboratory personnel, knowledge about critical AMR and carbapenemase producing Enterobacterales detection was higher in post-intervention (65.3% and 79.2%, p = 0.10 and 57.1% and 67.3% p = 0.03, respectively, Table 2b).In both studies, fewer than half of the staff were aware of the procedures to follow after the identification of critical AMR organisms.Knowledge on the use of genomics for understanding transmission pathways of AMR organisms and the procedures to store bacterial isolates for whole genome sequencing remained unchanged (Table 2b).

Attitudes
In both studies, almost all nurses and doctors agreed that it is important to collect data on critical AMR in CWMH (98.5% in pre-and 98.9% in post-intervention) and reported their willingness to change their work environment to prevent or control critical AMR infections (84.2% in pre-and 84.8% in post-intervention, p = 0.13) (Table 3).Over 90% of HCW who responded in both time periods reported that they believed overcrowding in CWMH increased the spread of AMR infections.Most HCW reported that they thought critical AMR organisms were common among hospitalised patients (74.1 in pre-and 81.1% in post-intervention, p = 0.06) and recognised that AMR infections could also occur in community settings (78.4% in pre-and 86.4% in post-intervention, p = 0.002) however less than a quarter of HCW (20.1% in pre and 22.2% in post-intervention) were aware that critical AMR can be a problem among international travelers.Most laboratory staff felt comfortable working in the laboratory where critical AMR organisms are processed (79.6 in pre-and 75.5% in post-intervention, p = 0.27).Among

Readiness
With regard to readiness, 41.3% of respondents in the pre-and 52.3% in the post-intervention study (p = 0.01) agreed that the hospital laboratory had adequate capacity to detect critical AMR organisms (Table 4).In both studies, nearly half of the doctors and nurses reported that the hospital did not have adequate capacity to respond to an outbreak of critical AMR infections (44.0% in the pre-and 51.9% in the post-intervention, ( p = 0.01) and reported that there were insufficient resources for management of patients with critical AMR infections (38.9% in pre-and 44.4% in post-intervention, p = 0.01).The proportion of HCW who were aware of AMR surveillance and reporting systems increased from 51.7% in the preintervention to 66.3% in the post-intervention, p < 0.001.Similarly, in the post-intervention study, more nurses and doctors reported that data on critical AMR organisms was readily available to them (29.4% in pre-and 52.4% in post-intervention cohorts, p < 0.001).

Discussion
This study assessed the knowledge, attitudes, and readiness about critical AMR and IPC among 813 different professional staff including doctors, nurses, and laboratory personnel in Fiji's main tertiary hospital.It found improvements in knowledge and attitudes to manage this problem after a dedicated educational campaign, but importantly more work is needed to reach high levels of organisational readiness for outbreaks in particular.Interestingly this study happened over a time a significant movement of nursing workforce in Fiji [27,28].As a result, a more junior nursing staff cohort participated in the post-intervention study.However, the education and training program was still associated with staff reporting feeling better prepared to manage critical AMR.HCW awareness of IPC is of paramount importance for effectively addressing the burden of AMR.In our study, HCW reported good knowledge of standard and TBP as well as hand hygiene.These findings contrast with studies in India [12] and Ethiopia [13] where only 27% and 41% of HCW reported understanding TBP and hand hygiene, respectively.The results from Fiji were closer to a multicounty study in Europe [29] (86.9%) and slightly higher than a Zambian study (61%) among nurses [14].
This study demonstrated significant improvements in HCW knowledge following targeted interventions.Nearly half of post-intervention participants reported receiving IPC and AMR information and education.At post-intervention 93.5% of surveyed HCW were aware that critical AMR organisms had been reported in the hospital.More nurses and doctors reported that they were confident in their IPC knowledge and knew what to do for patients with critical AMR infections.This awareness is a fundamental step toward implementing effective Interestingly although HCW acknowledged AMR can occur in hospitals and in the community, many respondents believed AMR is not a problem among international travellers.Studies have shown travel to AMR prevalent countries increases the risk of colonisation with MDRO [30,31] or acquiring an AMR infection if accessing medical facilities [32].Fiji has an overseas medical referral system to countries where the AMR burden is high [33] and repatriates many patients to CWMH after these visits.More education is needed to raise awareness on the increased risk of AMR among returning travellers and patients.CWMH is developing standardised procedures to promptly identify, and screen patients deemed to be at high-risk for colonisation or infection because of overseas travel or treatment.
Adoption of globally recommended IPC measures including contact precautions, hand hygiene and appropriate environmental cleaning is effective in reducing the spread of critical AMR organisms in health care settings [10,34].The risk of transmission of CRO to HCW is believed to be minimal [10].However, in both time periods, around one third of nurses and doctors expressed their discomfort at working with patients with critical AMR infections.Our study did not investigate the reasons for HCW's concerns however, provision of adequate and uninterrupted supply of personal protective equipment, environmental cleaning and disinfection products, and hand hygiene consumables are important as well as ongoing education and proactive positive attitudes of staff.
Translation of knowledge and attitudes to changes in practice is a major challenge [26,29,[35][36][37].Our study didn't investigate IPC practice.But contact precautions compliance audits conducted during the project implementation showed poor practices such as with hand hygiene compliance and use of PPE (Additional file 1 Table 3).Individual and group feedback were given to staff and IPC team throughout the project implementation so that local leaders (particularly nursing and ICU team leaders) were made aware of the performance of their ward in audits.Previous studies have demonstrated that education and training of HCW on IPC could improve IPC practices and reduce HAI [38] and the incidence of AMR [39,40].However, multiple confounding factors influence IPC compliance, including shortages of consumables, poor infrastructure, and high staff workloads [29,35,36,41,42].In Fiji, a large number of HCW including nurses have migrated overseas or joined the private health sector since 2022 [27,28].With the departure of experienced HCW, the posts will often be filled by junior staff.Hence there is a need for ongoing education and capacity building activities to sustain improvement.Further major challenges for effective IPC in CWMH include inadequate equipment and infrastructure [19,20].Ongoing attention to address these barriers is needed to mitigate the risk and impact of critical AMR.
Most HCW respondents identified insufficient hospital capacity for laboratory detection, surveillance, outbreak response and treatment of critical AMR organisms as key ongoing gaps needing further attention.These findings are similar to previous studies in the Western Pacific region which highlighted a lack of national AMR surveillance programs (54.8%) and insufficient laboratory capacity for AMR detection (51.1%) [23].Outbreak response requires coordinated systems, which local clinicians are working to build in Fiji.Access to last line antimicrobials to treat these infections is challenging, and many of these drugs are costly which may pose major difficulties for low-and middle-income countries.
The study findings should be interpreted in view of its limitations.Firstly, we used a convenience sampling method whereby readily available HCW were invited to participate.Also due to the departure of many senior nurses in 2022 and 2023, the post-intervention study enrolled more junior nurses compared to pre-intervention study.These two factors may have introduced selection bias which may have affected our results.Secondly, our results are based on self-reported responses, and we are unable to rule out possible over or under reporting.Thirdly, our studies did not investigate IPC practices, therefore improvement in knowledge and attitudes in the post intervention study does not necessarily signify better IPC practices as a result of the project's capacity building activities.Lastly, the studies were conducted in one of the tertiary hospitals with high rate of AMR therefore results cannot be generalised for the whole of Fijian facilities.
In conclusion, our data revealed good AMR and IPC knowledge and attitudes.However, staff perceived that surveillance, readiness for outbreak response and treatment of patients with critical AMR organisms were inadequate.Improving laboratory and IPC capabilities for critical AMR in CWMH will require sustained and multisectoral interventions with strong administrative commitment.Such commitment should include recognition of AMR and IPC as priorities and availing the required human, financial and material resources needed to promote good IPC practices.Strengthening AMR governance requires establishing guidelines and standardisation for AMR surveillance, reporting and outbreak response.Integration of AMR and IPC topics into the continuing medical and nursing education will help to enable sustained HCW training and education.

Table 1
Demographic and professional information of healthcare workers ‡Comparison of the pre and post intervention findings, *Others include infection control, clinical governance, oncology, and outpatient clinics, ICU = Intensive care unit, CCU = Cardiac care unit, Obs/Gyn = obstetrics and gynaecology, IPC = infection prevention and control, AMR = antimicrobial resistance

Table 2a
Knowledge of nurses, doctors, and laboratory personnel on infection control and AMR

know what to do if a patient with CAR infection is admitted in the ward.
85% in pre-and ≥ 95% in post-intervention) knew that AMR organisms can be transmitted from one patient to another, via HCW contaminated hands to patients, as well as via contaminated equipment.

Information on critical AMR infection is readily to laboratory staff
‡Comparison of the pre and post intervention findings, *Fisher exact test nurses and doctors the proportions were lower (61.9% in pre-and 63.5% in post-intervention, p = 0.74) compared to laboratory staff.In both time periods, around 30% reported that they were not comfortable working in wards or ICUs where patients with infections due to critical AMR organisms are admitted.In total, 94% of nurses and doctors recognised low risk of transmission of critical AMR to staff when caring for patients with AMR organisms.Significantly more nurses and doctors believed that they had adequate knowledge for critical AMR IPC in the post-intervention time period (63.4% in pre-and 81.4% in post-intervention, < 0.001).

Table 3
Attitudes of nurses, doctors, and laboratory personnel responses on infection control and AMR

Table 4
Readiness of nurses, doctors, and laboratory personnel on AMR

Adequate critical AMR surveillance data is readily available for doctors and nurses
‡Comparison of the pre and post intervention findings containment measures.These improvements are likely to reflect the positive impact of the twelve months of integrated education and training provided by the intervention.There were some areas such as laboratory staff knowledge about surveillance, and genomics that likely need further dedicated attention.