Phenotypic and genotypic characterization of antibiotic resistance in the methicillin-resistant Staphylococcus aureus strains isolated from hospital cockroaches

Cockroaches are one of the most important and frequent insects responsible for harboring, transmission and dissemination of human pathogens in the hospital environment. The present research was done to study the phenotypic and genotypic characterization of antibiotic resistance in the Methicillin-resistant Staphylococcus aureus strains isolated from hospital cockroaches. Five-hundred and thirty Periplanets americana and Blattella germanica cockroaches were collected and their gut content and external washing samples were subjected to bacterial isolation. MRSA strains were subjected to disk diffusion and PCR amplification of antibiotic resistance genes. Prevalence of MRSA strains in P. americana and B. germanica cockroaches were 52.77 and 43.33%, respectively. External washing samples of P. americana cockroaches had the highest prevalence of MRSA strains (59.57%). MRSA isolates of external washing samples harbored the highest prevalence of resistance against penicillin (100%), ceftaroline (100%), tetracycline (100%), gentamicin (83.33%) and trimethoprim-sulfamethoxazole (80.55%). MRSA strains isolated from gut content samples harbored the highest prevalence of resistance against penicillin (100%), ceftaroline (100%), tetracycline (100%), trimethoprim-sulfamethoxazole (80%) and gentamicin (73.33%). BlaZ, aacA-D, tetK, msrA, dfrA, ermA, gyrA, grlA and rpoB were the most commonly detected antibiotic resistance genes amongst the MRSA strains. The present investigation is the first report of the phenotypic and genotypic evaluation of antibiotic resistance in the MRSA strains isolated from P. americana and B. germanica hospital cockroaches. Hospital cockroaches are considered as a potential mechanical vector for MRSA strains.


Introduction
There are several ways to increase the survival of microorganisms in the environment and their transmission to human population. Cockroaches are among the most common insects found in industrial and residential environments such as hospitals. Indeed, the species has been highly successful in exploiting niches within human habitation. In addition to being a persistent pest and owing to their omnivorous habit of feeding and indiscriminate deposition of fecal materials, cockroaches are well-known agents for harboring, transmission and dissemination of human pathogens, thereby representing a public health risk [1,2].
There are two cockroach species commonly found infesting in domestic, industrial and residential environments, these are the German cockroach (Blattella germanica (B. germanica)) and the American cockroach (Periplanets americana (P. americana)) (Dictyoptera; Blattidae). P. americana is the largest shiny reddish brown peridomestic cockroaches measuring on average 4-5 cm in length, while B. germanica is light yellowish brown with length ranging from 1.0-2.5 cm [1,2]. B. germanica are the most abundantly distributed cockroach species. B. germanica are more prevalent inside the home, while the P. americana are common around the home and associated with water drainage systems and water pipes. Both of them can disseminate through the sewage system especially in public places such as hospitals [1,2].
Over 100 species of virulent and resistant bacteria have been isolated from or passed through cockroaches. Microbiological and epidemiological investigations reported that Staphylococcus aureus (S. aureus) has commonly been isolated from P. americana and B. germanica found in the public places and especially hospital environment [3,4]. S. aureus is commonly found in nose and respiratory system and on the skin [5]. It is responsible for the occurrence of nosocomial and community-acquired infections, food-borne diseases and food poisoning [5].
S. aureus strains are usually resist against several types of antibiotics [6][7][8][9]. Nowadays, methicillin-resistant S. aureus (MRSA) has become a serious problem in hospitals [6][7][8][9]. Documented data revealed that about 50-70% of the S. aureus strains isolated from the hospital environment were MRSA [6][7][8][9]. MRSA strains are responsible for about 100,000 cases of infections with around 20% mortality rate each year in the United States [10]. High pathogenicity of MRSA strains, its high resistance to several types of antibiotics and its nosocomial aspects have increased the importance of isolation of MRSA from the hospital cockroaches. MRSA strains are believed to serve as important reservoirs of antimicrobial resistance genes which can transfer and integrate into the MRSA genome leading to the emergence of new and potentially more resistant strains [6][7][8][9]. Documented data revealed that presence of certain antibiotic resistance genes is responsible for occurrence of severe antibiotic resistance [6][7][8][9]. Reports showed the high presence of rpoB, blaZ, mecA, aacA-D, tetK and tetM, ermA and msrA, linA, vatA and vatB, dfrA, gyrA and grlA and cat1 antibiotic resistance genes in the S. aureus strains caused severe occurrence of resistance against ansamycins, penicillins, methicillin, aminoglycosides, tetracyclines, macrolides-lincosamide-streptogramin B, lincosamides, streptogramins, folate inhibitors, fluoroquinolones and phenicols groups of antibiotics, respectively [6][7][8][9]. Reports of methicillin-resistant strains are challenging due to the large proportion of methicillin-resistant strains and increasing numbers of isolates reinforcing the need to revise their importance to hospital [6][7][8][9]. Therefore, screening of these elements is important for public health and despite the importance of such a screen, limited data are available for MRSA at the species level among the hospital cockroach samples.
MRSA strains have been tested in hospital cockroach samples to assess microbiological safety, sanitation conditions and finally epidemiological roles of these insects in survival and transmission of bacteria. High pathogenicity of MRSA strains and general weakness of hospitalized patients make it necessary to assess the presence of MRSA strains in hospital cockroach samples. Thus, the current research was done to study the phenotypic and genotypic properties of antibiotic resistance in the MRSA strains isolated from P. americana and B. germanica cockroaches in Iranian hospitals.

Samples
The present descriptive study was conducted during 2016 and 2017 at the tertiary hospitals of the Tehran province, Iran. Five-hundred and thirty cockroaches were collected using sticky traps, vacuum cleaners and hand catch methods from human dwellings. Traps were manufactured according to the design of Reierson and Rust (1997) [11]. The traps were placed on the floor under beds, cupboards, wooden racks, and/or benches, for two consecutive days. Each trapped cockroach was placed in a sterile test tube before sending to the laboratory. After immobilization by freezing at 0°C for 5 min, the species of cockroaches were identified under a dissecting microscope according to Harwood and James (1979) [12]. P. americana and B. germanica cockroaches were subjected to further parts of the study.

Sample preparation and isolation of S. aureus
Body surface of cockroaches were washed with physiological saline after vortexing for 2 min and taken as a homogenate sample. Before gastrointestinal tract (GIT) dissection, each cockroach was decontaminated with 95% ethanol, and the residue of ethanol was removed with saline solution. The gut was dissected aseptically using sterile needles and washed with 5-mL normal saline solution. Caution was taken to reduce the number of cut off or break in the gut. One milliliter of each homogenate was inoculated separately into 9-mL of buffered peptone water (Merck, Germany) for primary enrichment, and incubated at 37°C for 18-24 h. Samples were then inoculated in screw cap test tube containing nutrient broth (NB, Merck, Germany) for the isolation of S. aureus, and enriched overnight at 37°C. A loopful of inoculum from the enriched culture was streaked on to Blood Agar (BA, Merck, Germany) and Mannitol Salt Agar (MSA, Merck, Germany) incubated at 37°C for 24 h for the observation of hemolysis and mannitol fermentation, respectively. Colonies with beta-hemolysis reaction and mannitol fermentation were further identified on the basis of Gram staining, catalase activity, coagulated test (rabbit plasma), oxidase test, glucose O/F test, resistance to bacitracin (0.04 U), urease activity, nitrate reduction, phosphatase, deoxyribonuclease (DNase, Merck, Germany) test, voges-proskaver (Merck, Germany) test and carbohydrate (xylose, sucrose, trehalose and maltose, fructose, lactose, mannose) fermentation tests [13].

Identification of methicillin-resistant Staphylococcus aureus strains
Cefoxitin (30 μg) and oxacillin (1 μg) susceptibility tests were used to distinguish the MRSA strains from S. aureus isolates of hospital cockroaches. All tests were performed using the guidelines of the Clinical and Laboratory Standards Institute (CLSI) [14].
MRSA isolates were identified another time using the PCR-based amplification of mecA gene. MRSA strains were sub-cultured on TSB media (Merck, Germany) and further incubated for 48 h at 37°C. Genomic DNA was extracted from bacterial colonies using the DNA extraction kit (Thermo Fisher Scientific, St. Leon-Rot, Germany) according to manufacturer's instruction. Purity (A260/A280) and concentration of extracted DNA were then checked (NanoDrop, Thermo Scientific, Waltham, MA, USA). The truth of the DNA was assessed on a 2% agarose gel stained with ethidium bromide (0.5 μg/mL) (Thermo Fisher Scientific, St. Leon-Rot, Germany).

Phenotypic evaluation of antibiotic resistance
Patterns of antimicrobial resistance of the MRSA strains isolated from hospital cockroaches were studied using the simple disk diffusion according to the Kirby-Baur disc diffusion technique. The Mueller-Hinton agar (Merck, Germany) medium was used for this purpose.

Statistical analysis
Statistical analysis was done using the SPSS 21.0 statistical software (SPSS Inc., Chicago, IL, USA). Chi-square test and Fisher's exact two-tailed test were used to assess any significant relationship between prevalence of S. aureus and MRSA strains and their phenotypic and genotypic properties of antibiotic resistance. P value < 0.05 was considered as statistical significant level. Table 2 represents the prevalence of S. aureus and MRSA strains in different types of hospital cockroaches. Sixty-five out of 530 (12.26%) external washing samples of hospital cockroaches and thirty-seven out of 530 (6.98%) gut content samples of hospital cockroaches were positive for S. aureus strains. Prevalence of S. aureus strains in P. americana and B. germanica hospital cockroaches were 11.61% (72/620) and 6.81% (30/440), respectively. External washing samples of P. americana Table 1 Target genes, oligonucleotide primers and PCR conditions used for detection of antibiotic resistance genes in the MRSA strains isolated from hospital cockroaches (16)(17)(18)(19)(20)(21)(22) Target gene Primer sequence (5′-3′) PCR product (bp) PCR programs PCR volume (  Prevalence of S. aureus strains in external washing and gut content samples of B. germanica hospital cockroaches were 8.18 and 5.45%, respectively. Statistically significant differences were seen between types of hospital cockroaches and prevalence of S. aureus (P < 0.05). MRSA strains were further identified using the mecA gene PCR amplification. Figure 1 shows the gel electrophoresis of the mecA gene of the MRSA strains in PCR reaction. Thirty-six out of 65 (55.38%) S. aureus strains isolated from external washing samples of hospital cockroaches and fifteen out of 37 (40.54%) S. aureus strains isolated from gut content samples of hospital cockroaches were confirmed to be MRSA strains. Prevalence of MRSA strains in P. americana and B. germanica hospital cockroaches were 52.77% (38/72) and 43.33% (13/ 30), respectively. External washing samples of P. americana hospital cockroaches had the highest prevalence of MRSA strains (59.57%), while gut content samples of P. americana hospital cockroaches had the lowest (40%). Prevalence of MRSA strains in external washing and gut content samples of B. germanica hospital cockroaches were 44.44 and 41.66%, respectively. Statistically significant differences were seen between types of hospital cockroaches and prevalence of MRSA (P < 0.05).

Results
MRSA isolates were subjected to disk diffusion to assess the antibiotic resistance pattern. Table 3 represents the phenotypic pattern of antibiotic resistance of MRSA strains isolated from hospital cockroaches. MRSA strains isolated from external washing samples of hospital cockroaches harbored the highest prevalence of resistance against penicillin (100%), ceftaroline (100%), tetracycline (100%), gentamicin (83.33%) and trimethoprim-sulfamethoxazole (80.55%) antibiotic agents. MRSA strains isolated from gut content samples of hospital cockroaches harbored the highest prevalence of resistance against penicillin (100%), ceftaroline (100%), tetracycline (100%), trimethoprim-sulfamethoxazole (80%) and gentamicin (73.33%) antibiotic agents. MRSA strains isolated from external washing samples of hospital cockroaches harbored the lowest prevalence of resistance against chloramphenicol (25%), rifampin (33.33%), levofloxacin (38.88%) and ciprofloxacin (44.44%) antibiotic agents. MRSA strains isolated from gut content samples of hospital cockroaches harbored the lowest prevalence of resistance against chloramphenicol (20%), rifampin (33.33%), levofloxacin (40%), ciprofloxacin (46.66%) and clindamycin (46.66%) antibiotic agents. MRSA strains isolated from P. americana harbored the higher prevalence of resistance against almost all antibiotic agents (P < 0.05). Additionally, those of external   washing samples had the higher prevalence of resistance against almost all tested antibiotic agents (P < 0.05). Statistically significant differences were seen between types of samples and prevalence of antibiotic resistance (P < 0.05). Figure 2 represents the prevalence of resistant MRSA strains in different types of hospital cockroaches. Multi-drug resistant strains were determined as those who had at least simultaneous resistance against 3 or more than 3 classes of antibiotics. All of the MRSA strains isolated from external washing samples of hospital cockroaches at least had resistance against 3 different classes of antibiotics, while prevalence of resistance against more than 8 classes of antibiotics was 13.88%. Additionally, 86.66% of the MRSA strains isolated from gut content samples of hospital cockroaches at least had resistance against 3 different classes of antibiotics, while prevalence of resistance against more than 8 classes of antibiotics was 6.66%. Figure 3 represents the prevalence of resistant MRSA strains in P. americana and B. germanica hospital cockroaches. All of the MRSA strains isolated from P. americana hospital cockroaches at least had resistance against 3 different classes of antibiotics, while prevalence of resistance against more than 8 classes of antibiotics was 14.28%. Additionally, 84.61% of the MRSA strains isolated from B. germanica hospital cockroaches at least had resistance against 3 different classes of antibiotics, while prevalence of resistance against more than 8 classes of antibiotics was 7.69%. Table 4 represents the genotypic pattern of antibiotic resistance amongst the MRSA strain isolated from different types of hospital cockroaches. We found that blaZ (100%), aacA-D (88.88%), tetK (72.22%), msrA (63.88%), and dfrA (63.88%) were the most commonly detected antibiotic resistance genes amongst the MRSA strains isolated from external washing of hospital cockroaches. Additionally, blaZ (100%), aacA-D (73.33%), msrA (66.66%), tetK (60%) and dfrA (60%) were the most commonly detected antibiotic resistance genes amongst the MRSA strains isolated from gut contents of hospital cockroaches. We found that vatB (11.11%), cat1 (19.44%), tetM (27.77%), grlA (27.77%), rpoB (27.77%) and vatA (30.55%) had the lowest distribution amongst the antibiotic resistance genes in MRSA strains isolated from external washing of hospital cockroaches. Additionally, tetM (20%), linA (20%), ermA (20%), vatB (20%), grlA (20%), rpoB (20%) and cat1 (20%) had the lowest distribution amongst the antibiotic resistance genes in MRSA strains isolated from gut contents of hospital cockroaches. MRSA strains isolated from P. americana harbored the higher prevalence of antibiotic resistance genes (P < 0.05). Additionally, those of external washing had the higher prevalence of antibiotic resistance genes (P < 0.05). Statistically significant differences were seen between types of samples and prevalence of antibiotic resistance genes (P < 0.05).

Discussion
Cockroaches are among the most notorious pests, having nocturnal and filthy habits, which do not only contaminate food by indiscriminate deposition of fecal materials and cause food poisoning but also transmit bacteria and other pathogenic microorganisms in infested areas. Cockroaches are common in many of human habitations, particularly in place where food is stored, processed, prepared, or served. Apart from that, they are also frequently detected in hospital environments, such as wards, operational rooms, area of intensive care units, and laboratory rooms. Indeed, cockroaches are found everywhere and possess nocturnal and omnivorous features; these characteristics make them the ideal carriers of pathogenic, virulent and resistant microorganisms and especially S. aureus [23].
The present investigation showed that 12.26% of external washing and 6.98% of gut content samples of hospital cockroaches were positive for S. aureus strains. Additionally, the prevalence of MRSA strains in external washing and gut content samples of hospital cockroaches were 6.79% (36/530) and 2.83% (15/530), respectively. Furthermore, P. americana cockroaches had the higher prevalence of S. aureus and also MRSA    strains. Findings represents an important public health issue regarding the presence of B. germanica and P. americana cockroaches in hospital environment and also their high importance as a dangerous vector for survival and dissemination of S. aureus and also MRSA strains in the hospital environment. Findings also represent the higher prevalence of S. aureus and also MRSA strains in external washing samples of studied hospital cockroaches which may signify the higher role of external parts of the hospital cockroaches for dissemination of S. aureus and also MRSA strains. Moreover, S. aureus is not intestinal bacterium with high distribution in gut contents of the hospital cockroaches. However, presence of S. aureus in gut content samples of hospital cockroaches may be due to the permanent presence of S. aureus in hospital food samples [6,7,24] which may be consumed by cockroaches. Higher prevalence of S. aureus and MRSA in P. americana is may be due to their      (20) 3 (20) higher presence in hospital sewage than B. germanica cockroaches. Although P. americana is three to four folds larger than B. germanica in length, the capability of harboring microorganisms in cockroaches is not only related to their sizes but may also depend on the sanitation conditions of the environment.
Research on S. aureus carried by cockroaches in hospital environments is very important in relation to the control of nosocomial infections, which are becoming a major challenge. These infections cause high morbidity and mortality and increased hospitalization time having a consequent increase in costs. Cockroaches are able to explore various spaces in a hospital environment, making them a potential health risk, due to their ability to disperse pathogenic strains. Their growth is facilitated by fluids and food, as well as structural flaws in the hospital environment. Hospital infection is a major challenge for health professionals working in this area. The need to control and limit cockroaches has been stressed by most researchers.
MRSA strains isolated from hospital cockroaches harbored relatively high prevalence of resistance against tested antibiotic agents. MRSA strains harbored the highest prevalence of resistance against penicillin, ceftaroline, tetracycline, gentamicin, trimethoprim-sulfamethoxazole antibiotic agents. Additionally, prevalence of blaZ, aacA-D, tetK, msrA and dfrA was relatively higher than other tested antibiotic resistance genes. We found that phenotypic properties of antibiotic resistance were approved using the genotypic characterization of antibiotic resistance. MRSA strains isolated from P. americana cockroaches and also external washing samples had the higher prevalence of antibiotic resistance and antibiotic resistance genes. We also found the higher prevalence of resistance against human-based antibiotic agents in the MRSA strains isolated from hospital cockroaches which may indirectly show transmission of resistant MRSA strains from infected patients hospitalized in hospitals and health care units to resident cockroaches of hospitals. Antibiotics are not normally applied on cockroaches, but it is known that high resistance rates were reported among pathogens associated foods. In fact, a great association between cockroaches and foods could be the probable reason for isolation of resistant strains from cockroaches. Our findings revealed that more than 50% of S. aureus isolates were MRSA. High prevalence of antibiotic resistance reported in the present study is may be due to the unauthorized and indiscriminate prescription of antibiotic agents in Iranian hospitals and health care units. The circumstances in developing countries like Iran may be inflated by easy convenience of antimicrobials at a cheaper price and their extensive use in medicine.
We found that all of the MRSA strains were positive for the mecA gene. Most of the tetracycline-resistant MRSA isolates harbored tetK and tetM genes. Prevalence of aacA-D gene was high among gentamicin and amikacin-resistant MRSA strains. Prevalence of msrA, ermA and linA antibiotic resistance genes were also significant among the macrolide, erythromycin and clindamycin-resistant MRSA strains. Additionally, high prevalence of dfrA, rpoB and cat1 antibiotic resistance genes were also found amongst the trimethoprim-sulfamethoxazole, rifampin and chloramphenicol-resistant MRSA strains. Finally, high distribution of gyrA and grlA antibiotic resistance genes were found amongst the ciprofloxacin and levofloxacin-resistant MRSA strains. Therefore, the pattern of the antibiotic resistance of the MRSA strains of hospital cockroach samples was confirmed by the PCR amplification of the specific antibiotic resistance genes. MRSA strains of our study had considerable prevalence of resistance against clindamycin (44.44 to 46.66%). The most imperative mechanism involving resistance against clindamycin is modulated by methylase enzyme which often encoded by ermA gene [42]. Prevalence of ermA antibiotic resistance genes among the MRSA strains of our research were 46.66 to 50%. Majority of our isolates carried two tetracyclines, two erythromycins, one macrolide and several streptogramin resistance determinants reveals a great diffusion of these types of resistance. TetK, ermA, msrA and aacA-D which encode resistance against tetracycline, erythromycin, macrolides and aminoglycosides were the most commonly detected antibiotic resistance genes in the MRSA strains isolated of hospital cockroach samples. Furthermore, prevalence of blaZ, dfrA and gyrA antibiotic resistance genes which encode resistance against penicillins, folate inhibitors and fluoroquinolones, respectively. The literature survey did not indicate any report on the prevalence of blaZ, rpoB, gyrA, grlA, vatA, vatB, vatC, dfrA, cat1, msrA, ermA, linA, aacA-D, tetK and tetM genes among the MRSA strains of hospital cockroach samples. Kumar et al. (2010) [43] reported that the most commonly identified antibiotic resistance genes among the S. aureus isolates were linA (51.60%), msrB (46.10%), tetK (34.40%), tetM (34.40%), msrA (26.60%) and aacA-D (26.60%). Karataş et al. (2017) [44] revealed the higher prevalence of ermA than ermc antibiotic resistance genes among the clindamycin, erythromycin and telithromycin-resistant and also higher prevalence of tetM than tetK antibiotic resistance genes among the tetracycline-resistant MRSA strains which were similar to our findings. Our results were also similar with those of the previous research which was conducted by Simeoni et al. (2008) [45]. They reported that the prevalence of tetM, tetO, tetK, ermA, ermB, ermC, aac, blaZ and mecA antibiotic resistance genes amongst the S. aureus strains isolated from meat samples were 100, 0, 91.66, 16.66, 33.33, 58.33, 0, 100 and 58.33%, respectively. High prevalence of tetK and tetM antibiotic resistance genes in the MRSA isolates can be clarified by their usual genetic locations. Presence of tetK gene on small multicopy plasmids and tetM on conjugative transposons contributes to the spread of these determinants [46]. Some of the MRSA strains harbored ermA gene. This gene is often located on small multicopy plasmids which are present in many different staphylococcal species [46]. The ermA gene is usually carried by transposons which could explain its high prevalence amongst the MRSA strains. Resistance to aminoglycosides (60 to 83.33%) which encode by the aacA-D gene (73.33 to 88.88%) is also prevalent. Johler et al. (2011) [46] reported that prevalence of ermA, tetK and tetM antibiotic resistance genes among the S. aureus strains isolated from cases of food poisoning, milk and pork were 25, 4.87 and 0%, 50, 0 and 12.82% and 0, 12.19, and 53.84%, respectively. Podkowik et al. (2012) [47] reported that the prevalence of tetracycline resistance genes (tetO, tetK and tetM) and erythromycin resistance methylase gene (ermA, ermB and ermC) among the S. aureus strains were 44 and 60%, respectively. Prevalence of blaZ, rpoB, dfrA, gyrA, grlA and cat1 antibiotic resistance genes amongst the MRSA strains isolated from hospital cockroaches were 100%, 20-27.77%, 60-63.88%, 38.88-46.66%, 20-27.77% and 19.44-20%, respectively. High prevalence of blaZ, rpoB, dfrA, gyrA, grlA and cat1 antibiotic resistance genes was also reported in the S. aureus strains isolated from human clinical infection samples [48][49][50]. Resistance to benzylpenicillin is mainly caused by the blaZ gene encoding production of beta-lactamases, which hydrolytically destroy beta-lactams. Our results suggest that blaZ may play a major role in occurrence of resistance against penicillins but cannot be used alone as an indicator for penicillin resistance. Rifampin acts by interacting specifically with the β subunit of the bacterial RNA polymerase encoded by the rpoB gene. RpoB expression will increase the prevalence of resistance against rifampin in the S. aureus strains. Low prevalence of cat1 antibiotic resistance gene is may be due to the low levels of prescription of chloramphenicol in veterinary and medical sciences. Additionally, chloramphenicol is forbidden antibiotic agent in Iran. We also found the high prevalence of MRSA strains in the hospital cockroach samples. The multi-drug resistant S. aureus in cockroach was reported earlier from Nigeria, Ethiopia, Iran, and Brazil [3,4,[51][52][53]. Though this study tested multi-drug resistant against single bacterium, in the absence of prior work, the findings of this study would signify the emergence of MRSA in the environment and the prospective likelihood of dissemination of such strains through mechanical vector B. germanica and P. americana cockroaches in Iran.
MRSA strains are considered as important foodborne pathogens all-around the world. Thus, their high prevalence in hospital cockroaches may influence on their presence in food samples of hospitals. It is because of the fact that cockroaches can easily penetrate into the hospital food store units. High prevalence of S. aureus and other foodborne pathogens have also been reported previously [54][55][56][57][58][59][60][61][62][63][64].

Conclusions
The present investigation is the first report of the phenotypic and genotypic evaluation of antibiotic resistance in the MRSA strains isolated from external washing and gut content samples of B. germanica and P. americana hospital cockroaches. High prevalence of S. aureus and MRSA strains, higher prevalence of bacteria in P. americana cockroaches, higher prevalence of bacteria in external washing samples of cockroaches, high prevalence of resistance against penicillin, ceftaroline tetracycline, gentamicin and trimethoprim-sulfamethoxazole, high distribution of blaZ, aacA-D, tetK, msrA, dfrA, ermA, gyrA and grlA antibiotic resistance genes and higher prevalence of MRSA strains in external washing samples of P. americana cockroaches were the most important findings of the present study. The present study shows the high importance of hospital cockroaches as dangerous reservoirs for harboring of virulent and resistant MRSA strains in the hospital environment and their transmission to human population. Otherwise, external surface of the P. americana cockroaches can act as a mechanical vector for transmission of MRSA strains in the hospital environment in Iran. However, further studies are required to find additional knowledge about the microbiological and epidemiological roles of the hospital cockroaches in survival and transmission of antibiotic-resistant bacteria.