Dynamics expression of DmFKBP12/Calstabin during embryonic early development of Drosophila melanogaster

Calcium signaling are conserved from invertebrates to vertebrates and plays critical roles in many molecular mechanisms of embryogenesis and postnatal development. As a critical component of the signaling pathway, the RyR medicated calcium-induced calcium release signaling system, has been well studied along with their regulator FK506-binding protein 12 (FKBP12/Calstabin). Lack of FKBP12 is known to result in lethal cardiac dysfunction in mouse. However, precisely how FKBP12 is regulated and effects calcium signaling in Drosophila melanogaster remains largely unknown. In this study, we identified both temporal and localization changes in expression of DmFKBP12, a translational and transcriptional regulator of Drosophila RyR (DmRyR) and FKBP12, through embryonic development. DmFKBP12 is first expressed at the syncytial blastoderm stage and undergoes increased expression during the cellular blastoderm and early gastrulation stages. At late gastrulation, DmFKBP12 expression begins to decline until it reaches homeostasis, which it then maintains throughout the rest of development. Throughout these described changes in expression, DmFKBP12 mRNA remain stable, which indicates that protein dynamics are attributed to regulation at the mRNA to protein translation level. In addition to temporal changes in expression, dynamic expression profiles during Drosophila development also revealed DmFKBP12 localization. Although DmFKBP12 is distributed evenly between the anterior to posterior poles of the blastoderm egg, the protein is expressed more strongly in the cortex of the early Drosophila gastrula with the highest concentration found in the basement membrane of the cellular blastoderm. Fertilized egg, through the profile as under-membrane cortex distribution concentering onto basement at cellular blastoderm, to the profile as three-gem layer localization in primitive neuronal and digestion architecture of early Drosophila gastrula. By late gastrulation, DmFKBP12 is no longer identified in the yolk or lumen of duct structures and has relocated to the future brain (suboesophageal and supraesophageal ganglions), ventral nervous system, and muscular system. Throughout these changes in distribution, in situ DmFKBP12 mRNA monitoring detected equal distribution of DmFKBP12 mRNA, once again indicating that regulation of DmFKBP12 occurs at the translational level in Drosophila development. As a critical regulator of the DmRyR-FKBP complex, DmFKBP12 expression in Drosophila fluctuates temporally and geographically with the formation of organ systems. These finding indicate that DmFKBP12 and RyR associated calcium signaling plays an essential role in the successful development of Drosophila melanogaster. Further study on the differences between mammalian RyR-FKBP12 and Drosophila DmRyR-FKBP12 can be exploited to develop safe pesticides.


Introduction
In mammals, calcium signaling plays critical roles in many biological functions with its molecular mechanism in cell [1][2][3]. Abnormality of the signaling leads life-threatening diseases including cancers. Extracellular environmental homeostatic calcium is regulated through cell membrane integrated protein CaSR (calcium-sensing receptor) and ITG (integrins) [4,5], and cytoplasmic calcium is controlled by inositol 1,4,5-trisphosphate receptors (IP3R) plus ryanodine receptors (RyR) in endoplasmic reticulum (ER) via one of well-known calcium induced calcium release (CICR) signaling pathway [6][7][8][9]. The CICR pathway as one of important calcium signaling pathways in cell functions through binding with their regulators such as FKBP12 (also known as Calstabin) and FKBP12.6 [10][11][12][13][14][15]. In insect, Drosophila melanogaster ryanodine receptor (DmRyR) cDNA was cloned from lava and the physical features of DmRyR single channel were characterized with in vitro overexpression system [16]. As a unique isoform of RyR in insect, the binding protein DmFKBP12 is essential as well in insect physiological and cellular processes. FKBP proteins are FK506 binding protein family implicating with many cellular function including calcium receptor signaling, protein folding and trafficking, transaction control, apoptotic death, and up to physiological role including embryonic development, stress response, tumorigenesis, neuronal response, angiogenesis and vascular remodeling [6,7,17].
Previous study unveiled that, in Drosophila melanogaster, the eight known DmFKBPs share homology with the Homo sapiens FKBP12 among its paralogues and orthologues through molecular phylogenetic analysis of FKBP family proteins [17]. Apart from DmFKBP59 possessing two FKBP domains, all other DmFKBPs including DmFKBP12, DmFKBP13, DmFKBP14, DmFKBP39, Shutdown (CG4736) and other two unnamed DmFK-BPs (CG1847 and CG5482) compose of a single FKBP domain along with Cwf/Cwc superfamily domain, EFband domain and TPR domain individually according to references from NCBI to FlyBase [17]. Because lack of HmFKBP12 generating the newborn defects with the cerebral edema and cardiac arrhythmia [18], and FKBP12 overexpression led to lethal defect of arrhythmic pathology [19], a critical function of DmFKBP12 is expected on Drosophila physiological role. Recently, the data from DmFKBP12 mutant (also known as Calstabin and FK506-BP2) demonstrated that DmFKBP12 is necessary for S107 to play its critical role on extension of both health and life span against oxidative stress, and DmRyR is essential for larval development in Drosophila flies [20,21].
FKBP12 and FKBP12.6 are widely recognized as the regulators of RyRs associated many calcium signaling physiological function [6-8, 17, 21]. RyR conducted calcium sparks regulated by FKBP12 which were first found in activation of Medaka fish eggs [22,23] in zygote early development expressing critical function of FKBP12 [7]. To identify function of DmFKBP12 in early Drosophila development, in this study, we dynamically localized the distribution pattern of DmFKBP12 protein and mRNA at different embryonic stage. The information obtained in this research provides more comprehension on how DmFKBP12 performing its role within its dynamic distribution. Our data may benefit on developing more insect-specific pesticide targeting on early stage of insect embryo with more effective strategy related to DmRyR-FKBP12 complex.

Fly stocks
All Drosophila melanogaster flies and embryos were grown at 25 °C on standard cornmeal-molasses-agar medium until collection and fixation. The wild-type embryos were from Canton S strain. Embryos were staged as previously described [24]. All animals maintained in natural day-night cycle. All animal maintenance and experiments were performed under guidelines approved by the Animal Care and Use Committee of Xi'an for Animal Use of Universities in Shaanxi Province, P.R. China.

Immunohistochemistry
Deparaffinised and rehydrated sections were rinsed in PBS, and the antigens of samples were retrieved with preheated (approx. 100 °C) target retrieval solution (Dako, USA) for 20 min. Sections were treated with 0.3% H 2 O 2 in methanol for 10 min and followed by a blocking step with 1% bovine serum albumin (BSA) diluted in PBS for 60 min at room temperature (RT). Then sections were incubated with primary antibody (anti-FKBP12 at 1:1000, sc-28814, Santa Cruz). After washed with PBS, the Envision-plus detection system was applied with HRP labelled polymer conjugated with secondary antibodies (anti-Rabbit, EnVision + System-HRP, Dako). Reaction products were visualized after incubation with 3, 3′-diaminobenzidine [28,29].
The anti-FKBP12 antibody is a primary antibody designed against mouse FKBP12. And this antibody can also recognizes Drosophila FKBP12 because of that mouse FKBP12 and Drosophila FKBP12 shares 77% identity of total sequence of amino acids, 87% positive of the amino acid sequences composed of acidic side chain, basic side chain and uncharged polar chain with 0% gaps evaluated by compositional matrix adjustment [17]. A FKBP12.6 comparison of Drosophila, human and mouse show below with polar acids S T Y N Q and C in light-gray highlight, positive charged acids R K and H in medium-gray highlight, and negative charged acids D and E in dark-gray highlight.
GCT AGC CGA TGG GCG TAC AAG TA  GTT CCA -3′)  and reverse primer (Primer-B, 5′-TAC GAG CTC CTA  TTC GAC CTT GAG CAG CTC -3′) were designed according to the published cDNA sequence of DmFKBP12 as FK506-binding protein 2 of Drosophila melanogaster (NM_079068.5). Specific sequences for identifying restriction enzyme Nhe I and Sac I were included in the primer pairs at the 5′ and 3′ ends as restriction sites, respectively. The expression of DmFKBP12 mRNA was assessed and the products were visualized with gel imaging system.

RNA in situ hybridization
Drosophila embryo cDNA was amplified by PCR with two primers (5′-CTA GCT AGC CGC CAC CAT GGG CGT ACA AGT AGT TCC A-3′, and 5′-TAC GAG CTC CGC CAC CTT C GAC CTT GAG CAG CTC-3′), and then cloned into linearized pGEM-T Easy plasmid vector to make circular plasmid vector with the probe cDNA under down stream of SP6 and T7 promoters. The probes were synthesized for RNA labeling with digoxigenin-UTP by in vitro transcription with SP6 and T7 RNA polymerase (#11175025910, Roche). Antisense double digoxigenin

Total RNA preparation and RT-PCR
Embryos were collected and snap frozen in liquid nitrogen. Total RNA was extracted with Trizol reagent (Invitrogen) and first-strand cDNA of DmFKBP12 was amplified from 3 µg of purified total RNA through reverse transcription polymerase chain reaction (RT-PCR) (Life Technologies) [9,30]. Forward primer (Primer-F, 5′-CTA (DIG) labeled RNA was used as a probe of DmFKBP12 hybridization, and DIG labeled sense RNA was used as a negative control (data not shown). The probes were quantified and applied 1 μg of probe per hybridization reaction. Probes were detected using a primary sheep anti-DIG-AP 1:10,000 (#11093274910, Roche) and NBT/ BCIP for the sensitive detection [31].
All Drosophila embryo sections collected at syncytial blastoderm, cellular blastoderm, early and late gastrulation stages, were prepared according to the previous description above with 0.01% DEPC pre-treated water [31]. DEPC overnight treatment was applied on all accessories used for the in situ hybridization.

Western blotting analysis
Expression analysis on the DmFKBP12 protein of Drosophila embryos was inspected by Western blotting according to the standard protocol [9,32]. Tissue proteins from embryos was extracted using RIPA reagent. Standard BSA approach was used for protein quantification. The proteins sample were denatured after heated at 100 °C and separated on a gradient SDS-PAGE, and then transferred onto polyvinylidene difluoride (PVDF) membranes with a BioRad transfer unit at 100 V for 120 min. The membranes were blocked with 5% nonfat milk in TBST at 4 °C overnight and detected with primary antibody (anti-β-tubulin 1:5000; anti-FKBP12 1:1000, sc-28814, Santa Cruz) at room temperature (RT). After three times washing with TBST, the membranes were incubated with secondary antibody at RT. Immunodetection was carried out with ECL followed by exposure to motored molecular imaging system (Tanon 4200, China). Images were quantitated using the Image J software downloaded from website of National Institutes of Health [16].

Microscopy and statistical analysis
The histochemistry images were documented and analyzed with the inverted microscopy (Carl Zeiss Microscopy GmbH) and manufacturer software ZEN. Data were presented as mean ± SEM, and were analyzed with SPSS 22.0 and Graphpad Prism 7. A one-way ANOVA followed by a post hoc comparison Tukey was employed to analyze the data. A P value less than 0.05 and 0.01 was considered statistically significant [16,27].

DmFKBP12 during Drosophila embryogenesis
The Drosophila embryonic samples were collected at four different stages i.e. syncytial blastoderm, cellular blastoderm, early and late gastrulation stages according to the approach described in the previous publication [33]. These four-stage Drosophila embryos harvested and sorted at following four timing-points for inspection of DmFKBP12 expression during Drosophila development. The embryos from syncytial blastoderm stage were sorted from collection of 2 h after fertilization (syncytial blastoderm, 90.8%; n > 400). The embryos of cellular blastoderm stage (cellular blastoderm, 91.5%; n > 300), early gastrulation stage (early gastrula, 91.1%; n > 200) and late gastrulation stage (late gastrula, 93.3%; n > 200) were done individually from 3 h, 13 h and 24 h collection after fertilization.
The DmFKBP12 expression were detected within the Drosophila embryo lysis with Western blotting analysis in four stages (Fig. 1a). Normalized to the β-tubulin protein, the expression initiated at stage of syncytial blastoderm, increased at stage of cellular blastoderm (lane 1 and 2; p < 0.05) and kept constant at stage of early gastrula (p > 0.05) until stage of late gastrula (lane 3 and 4). In the late stage of gastrula embryos (lane 4), the DmFKBP12 expression dramatically decreased compared to syncytial and cellular blastoderm (both p < 0.001). Meanwhile, during the Drosophila gastrulation, the protein expression of DmFKBP12 in the late stage are dramatically decreased and kept at detectable level (lane 3 and 4; p < 0.001).
RT-PCR analysis of DmFKBP12 gene expression supported the result of protein analysis (Fig. 1b). In the total RNA samples of syncytial blastoderm, cellular blastoderm, early and late gastrulation stages, the DmFKBP12 cDNA fragment amplified from transcripted first strain cDNA were clearly detected in four-stage Drosophila embryos. The above inspection demonstrated that the Drosophila DmFKBP12 expression was examied at both translation and transcription levels. Our data extend the detected expression of DmFKBP12 from adult Drosophila [20] to very early embryonic syncytial blastoderm stage of its development with multiple nuclei of first 13 mitosis after fertilization [34].
As we know that DmFKBP12 is the regulator of DmRyR, the major component of Calcium signaling on CICR pathway, we further examined its existence within the Drosophila embryos by cloning its most conserved RyR domain of all RyR orthologues. The cDNA sequencing confirmation provided the evidence on the appearance of DmRyR in Drosophila embryos (Additional file 1: Figure S1).

Dynamic profile of DmFKBP12 protein from syncytial blastoderm to cellular blastoderm of the Drosophila embryo
Drosophila syncytial blastoderm is formed from zygote after 13 mitosis of two haploid pro-nuclear meeting [34]. After fertilization, the diploid nuclear DNA synthesis and rapid nuclear divisions do not generate daughter cells but daughter nuclei without accomplishment of the plasma cytokinesis, and lead to syncytial blastoderm in a multinuclear cell [34]. At this stage, the multinuclear blastoderm cell was equipped with evenly distributed yolk particles (Fig. 2a-d) and protein granules (Fig. 2i-l) composing of glycoproteins within the blastodermal cytoplasm. The DmFKBP12 protein can be detected in entire embryo but with less differentiated localization ( Fig. 2e-h, and q). The DmFKBP12 mainly localized in zones of both anterior pole (AP) and posterior pole (PP) of egg indicated with its density (Fig. 2q). The DmFKBP12 zone in posterior pole (Fig. 2h) is larger than the anterior pole ( Fig. 2f ) with weaker expression in middle zone of the egg (Fig. 2g, q). It is clear that the DmFKBP12 in both PP and AP zone are partially co-localized with glycoprotein granules. At the syncytial blastoderm stage, the single-cell Drosophila blastoderm with nuclear accumulation after 13 nucleic mitotic divisions are ready for cellularization process.
While Drosophila syncytial blastoderm develops to cellular blastoderm, the four thousands nuclei generated after 13 mitosis migrate to subsurface of the single-cell blastoderm egg (Fig. 3a-d) and form embryonic trophectoderm lineage, with central yolk sac zone fussed from spread small yolk portions. The lineage constructs embryo as classic blastodermal subsurface-linearized single nucleus-cell sheet enveloping the surface of embryo. At this stage, the differentiated distribution of the DmFKBP12 protein continuously exhibited in the Drosophila blastoderm. The positive staining shifted to cortex zone apposition next to trophectoderm primitive epithelium ( Fig. 3e-j, and u) with non-detectable staining in central yolk sac localization ( Fig. 3e-h, k) along with migration of glycoprotein granules ( Fig. 3l-o). With the development of the embryo, the DmFKBP12 proteins concentrated to basal-cell layer zone and aggregated in basement localization of blastodermal epithelial cells ( Fig. 3q-s, t), where is critical tissue architectural structure for directing embryo further differentiation. The protein density in basement membrane is dramatically higher than that in the cellularized cell layer (p < 0.0001) and significantly higher than that in the cortex zone as well (p < 0.001).

Dynamic profile of DmFKBP12 protein from early to late gastrulation stages of the Drosophila embryo
Dramatic differentiation in Drosophila embryo is promoted after accomplishment of cellular blastoderm in which "independent" cells after accomplishment of mitotic cytokinesis are configured from syncytial embryonic cells. In order to develop forwards to late Drosophila gastrula, the cellularized single-nuclear embryonic blastoderm cells began their migration continuously and arrived at their different location in early gastrulation  stage. In the early stage, the cells preformed their preliminary folding to form primary three-germ layers. The mouth of the posterior invagination was forced from anteriorly portion of embryo (Fig. 4a-d). The Drosophila DmFKBP12 were involved into all germ layer by locating in newly formed cells from the basement localization at the cellular blastoderm stage, and distributing in neuroblasts (NBL) and stomodaeum (ST) but not in yolk (YK) within zone of anterior pole, and hindgut (HG), germ band (GB), and posterior midgut rudiment (PMG) within zone of anterior pole of embryo ( Fig. 4e-h, and  m). At the late-early gastrulation stage, distributing trend of Drosophila DmFKBP12 appeared towards to brain, supraesophageal ganglion (SG), ventral nervous system (VNS), midgut (MG), and posterior midgut rudiment (PMG) but clearly not in yolk ( Fig. 4i-l, and m) with the equal densities in neuroblast and hindgut, higher expressions in germ band and posterior midgut rudiment compared to NBL and HG (Fig. 4m, all p < 0.01).
As the fundamental events of early differentiation, association of cell proliferation and migration are tightly relevant with cellular calcium signaling as discussed previously. It is noticeable that DmFKBP12 is essential within stage transition from early gastrula to late gastrula.
In late gastrulation stage, Drosophila embryo had relatively more distinguished complexity than that in the early and late-early stages on furthermore differentiation. Along with three-germ layers developing in gastrula, the detected expression of DmFKBP12 protein in the architecture of embryonic tissues and organs is getting more specific and restricted in certain location (Fig. 5). The expression was continuously detected in digestive, nervous and muscular system including mouth hook (MH), head muscle (MUC), pharyngeal musculature (PMUS), brain supraesophageal ganglion (BR), suboesophageal ganglion (GNSO), ventral nervous system (VNS), proventriculus (PV), midgut (MG), and salivary gland (SV) within zone of anterior pole and main body of embryo (Fig. 5a-h).
In Drosophila late gastrulation stage, a major recognizable difference from previous stages is that the non-distribution areas of the DmFKBP12 protein are much more noticeable beside yolk-zone surrounding central portion of nerve fibers and tracts (NF), luminal portion of the most tubular structures in entire body cavity (Fig. 5e-h). At the end of late gastrulation stage, the non-expression zone of DmFKBP12 protein is getting more and more discernible. Furthermore, inner germ layer differentiated organs/tissues expressing less DmFKBP12 are extremely eminent (Fig. 5i-l). Compared to our inspection on DmFKBP12 expression pattern in previous three stages of Drosophila development listed above, the protein distribution in the late gastrula is significant less than that in early gastrulation and other two stages according to our immunological detection. The DmFKBP12 protein expression distributed more in the cell-composed primary viscera including mouth hook, brain (supraesophageal ganglion), midgut caecum, proventriculus, anterior midgut rudipighian, middle midgut and posterior midgut rudiment, less located in their lumens (Fig. 5m, all p < 0.001). Weak signal of DmFKBP12 expressed in cavity of posterior and anterior poles can be detected.

Dynamic mRNA profile of DmFKBP12 gene expression of Drosophila embryogenesis from syncytial blastoderm via cellular blastoderm to gastrulation
As our dynamic data on expression of DmFKBP12 in Drosophila embryogenesis, the distribution pattern of DmFKBP12 protein developed from syncytial blastoderm, via cellular blastoderm, to early and late gastrulation stages along with growing construction of primitive tissues/organs from three-germ layers. All differentiation from a single-cell embryo, via syncytial thousand-nuclei cell embryo, to classic single-nuclear cell embryo after cellularization, the Drosophila development accompanying with cellular DmFKBP12 is critical on morphological architecture. In data of this session, we focus on investigation of DmFKBP12 gene expression on mRNA level to confirm the function of evolutional conceived DmFKBP12 gene in Drosophila embryogenesis.
We utilized RNA in situ hybridization analysis to dynamically inspect the mRNA of DmFKBP12 transcript from the Drosophila embryos harvested from four stages of syncytial blastoderm, cellular blastoderm and gastrulation including early and late gastrula (Fig. 6a-l). The DmFKBP12 mRNA were evenly distributed within entire syncytial blastodermal embryo and left yolk zone free  160 µm, for e, l and p, q is 80 µm, for b-d, f-h, m-o and r-s is 40 µm, for I- of the detected signal with multiple-small size stainless spots (Fig. 6a-c). In cellular blastodermal embryo, the mRNA were shifted towards embryo edge zone to subspace underneath cell membrane along with the moving of the thousands of nuclei, and eventually form the syncytial cell cortex zone and the central yolk zone. The massive DmFKBP12 mRNA staining distributed in the cortex zone but not in the central zone (Fig. 6d-f ). In the early gastrulation stage, three-germ layers started to be produced by aggravating towards inside of the embryo and push-shrink the DmFKBP12 mRNA free from the central yolk zone, the mRNA along with differentiated primitive germ layers were folded deeply towards into embryo to perform further cellular differentiation (Fig. 6g-i). While the Drosophila embryos development reached to late gastrula, three-germ layers already constructed more complicated tissues/organs with tracks and ducts of ventral nervous system and mouth hook, and luminal spaces of mouth hook, salivary gland, proventriculus and midgut rudiment. The DmFKBP12 mRNA staining always settled on solid portions of the tissues/organs including seating on primitive intra-epithelium of the structures and not within inner space of the primitive duct-tissues (Fig. 6j-l). Distribution of the DmFKBP12 mRNA staining signals appeared positively change with the condensed cells indicated by cell nuclei staining.
The above dynamic pattern on distribution of the DmFKBP12 mRNA was observed within Drosophila embryogenesis within four stages. In syncytial Fig. 5 Expression profile of DmFKBP12 protein at late gastrulation stage of the Drosophila embryo. a-d and i H&E staining of embryo at late gastrulation stage. A number of different morphogenic movements has been completed. e-h and j-l display the distribution of DmFKBP12 protein at late gastrulation stage. DmFKBP12 was detected in the differentiated organs which involved in digestive, nervous and muscular system, such as mouth hook, midgut, middle midgut, midgut caecum. Orientation of the embryo is anterior to the top and posterior to the bottom. i-l present advanced late gastrulae than that in e-h. The qualifications of DmFKBP12 distribution in early gastrulae are presented in m. Scale bar for a, e and i is 80 µm, for b-d, f-h and j-l is 40 µm. MH, mouth hook; SLG, salivary gland; GNSO, suboesophageal ganglion; BR, brain, supraesophageal ganglion; AMG, anterior midgut rudipighian; PMUS, pharyngeal musculature; PV, proventriculus; MG, midgut; VNS, ventral nervous system; NF, nerve fibers and tracts; MUS, muscle; YK, yolk; ST, stomodaeum; PMG, Posterior midgut rudiment; MGC, midgut caecum; MMG, middle midgut; TR, trachea. Data are expressed as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001 and ***p < 0.0001 blastoderm embryo, the mRNA signals were evenly distributed in both anterior pole and posterior pole (Figs. 6a-c, 7A). However, in cellular blastoderm embryo, the mRNA staining became un-even distribution with high density in anterior pole and less density in posterior pole (Figs. 6d-f, 7b). In early gastrulation stage, the un-even distribution became more noticeable by concentrating on neuronal tissue components in the zone of anterior pole, and less plus even expression of the DmFKBP12 mRNA in the zone of posterior pole   6g-i, 7c). In late gastrulation stage, the expression un-evenness and amount of the DmFKBP12 mRNA converted to less or non in connective space and lumen cavity, and more in tissue-specific architecture (Figs. 6j-l,  7d). The un-even expression of the DmFKBP12 mRNA in Drosophila embryo (Fig. 7a-d) immaculately supported the dynamic distribution of the DmFKBP12 protein presented the above data performed in four embryonic stages visualized with the patterns of protein density.

Discussion
RyRs plus IP3R are mainly responsible for calcium sparks in skeletal muscle, cardiac myocytes, smooth muscle, neurons and other excitation cells [6,7]. This calcium sparks can transform in different appearances or forms to perform their diverse functional molecular mechanism in cells of tissues where sparks locate. Ca 2+ releasing via single tetramer RyR generating Ca 2+ quark, via multiple adjacent complex producing Ca 2+ wave and ER/SR origin Ca 2+ local discretting Ca 2+ puff play critical role in myocytes [6,7]. In neuronal presynaptic terminal, RyR mediated Calcium releasing as Ca 2+ syntilla, through Ca 2+ synapse of a crossing-membrane structural and functional complex, are essential for neuronal transition [36,37]. In vertebrate, three isoforms of RyR comprehend their biological compound commission with molecular mechanism in cells, in which regulations from FKBP12 and/or FKBP12.6 is required. In this study, our data obtained with dynamic localization of DmFKBP12 protein and DmFKBP12 mRNA (Fig. 7), demonstrate functional essentiality of DmRyR-FKBP12 complex in embryonic development of Drosophila melanogaster.
It has been well recognized that FKBP12 and FKBP12.6 are critical regulators for Calcium-induced Calcium releasing through RyRs [6,38,39]. RyR1, RyR2 and RyR3 represent skeletal muscle specific isoform, cardiac isoform and brain specific isoform. Traditionally, FKBP12 is in charge of regulating skeletal muscle RyR1 Calcium channel, and FKBP12.6 interacts with cardiac muscle RyR2 by controlling EC coupling [38,39]. Because of recent data demonstrated that FKBP12 functions in transgenic heart with its cardiac overexpression by generating lethal arrhythmia [19,[39][40][41], it is more reasonable that multifunctional regulation of these two proteins on skeletal or/and cardiac RyR could be much miscellaneous or complexed at tissue specific cellular microenvironment. Recently, FKBP12 was linked to IP3R by regulating calcium release function in cancer cells [42]. The data indicated that FKBP12 could play as multi-functional regulator working for both RyR and IP3R in charge of associating these two critical calcium channel proteins while cell carrying on the pathophysiological function. It is certain that DmRyR-FKBP complex contributes its important role within Drosophila development according to our result although no report make sure yet on embryonic function of IP3R in Drosophila.
Drosophila homologs of two mammalian intracellular CICR Ca 2+ channels RyR and IP3R were first identified in the mesoderm of early stage-9 embryos, somatic precursor myocytes and neuronal tissues [42]. Then full-length of cDNA encoding DmRyR were cloned, and channel function of full-length and carboxyl-terminal with TMD channel portion were characterized systematically [16,43,44]. The critical function for larval development is characterized with hypomorphic allele RyR(16) mutation on muscle contraction and Ca 2+ channel activity [21]. Although a present work consistent to approve the importance DmRyR, for instance, that oxidative stress induced stem cell proliferation in Drosophila midgut through activating TRPA1/RyR-mediated Ca 2+ signaling, the DmFKBP12 as critical regulator of the Ca 2+ signaling are perceptibly less investigated (Fig. 8).
The Drosophila homolog of mammalian RyRs has been investigated previously, and comprehensions on DmRyR are relatively well known such as that an only RyR gene was identified in insect instead of three isoforms in mammalian [16,[42][43][44]. However, very limited information documented from published data about insect RyR regulator DmFKBP12. The DmFKBP12 was identified on function of extension both health and life spans Fig. 8 The Dynamic distribution of DmFKBP12/Calstabin protein in four development stage of Drosophila melanogaster. Full names of the developing tissues or organs were listed in Abbreviations by following standard terminology [45,46] using loss of DmFKBP12 gene function model in Drosophila muscle [20]. This work claimed that the absence of DmFKBP12 in the cytoplasmic of DmFKBP12 mutant muscle dissociated RyR from response to oxidation and resulted in earlier aging. Our data of DmFKBP12 cloning from earlier embryo (Fig. 1) extended that the function of DmFKBP12 perform its early contribution to fly embryo. The DmRyR-FKBP12 complex may already initiate their role not only in muscle but also in brain because DmRyR is only one RyR Drosophila homolog representative of mammalian three isoforms as we discussed above.
Molecular cloning of DmRyR cDNA provided the solid evidence of DmRyR expression in Drosophila lava [16]. As the crucial regulator of this CICR pathway along with binding protein of IP3R as well [47], in which four DmFKBP12 control opening of ER/SR DmRyR tetramer, the expression and function of DmFKBP12 should be carried out in the lava stage. Because of, as discussed above, RyR-mediated CICR exhibiting with calcium spark and calcium wave robusting from fertilization of many animals [48][49][50][51], it is judicious that the DmRyR-FKBP12 complex functions as early as the same stage. Our data in this investigation demonstrated that the DmFKBP12 distributed in subsurface cortex apposition to the plasma membrane in syncytial blastoderm (Figs. 1, 2, and 6a-c). We believe that the Drosophila DmRyR-FKBP12 as an ancient conserved complex performs its essential role throughout entire life of Drosophila melanogaster. Our data showed that the protein expression of Drosophila FKBP12 is significant different at four embryonic stages with the same mRNA level. This result strongly indicated that the existence of regulation pathway in the Drosophila embryonic development could link to the involvement of more proteins such as BAP1 and PTEN, which regulate by targeting on IP3R3-mediated Ca 2+ flux to mitochondria in mammal cell [52][53][54].
Furthermore, our results suggested a proficient strategy that binding ligands such as Tacrolimus (FK506), sirolimus (rapamycin) and chemicals derived from them, have potential to utilize on developing pesticide against early development stage Drosophila melanogaster by targeting on the embryonic DmRyR-FKBP12 complex. When tacrolimus and sirolimus as immunosuppressant molecule bind on FKBP proteins including DmFKBP12, this binding would decline or even fail the formation of DmRyR-FKBP12 or DmIP3R-FKBP12. Therefore, the cellular calcium signaling pathway would be disrupted accompanying with death of muscular and neuronal tissues while the dysfunction of DmRyR FKBP12 occurs on Drosophila embryo in four stages in development.

Conclusion
Calcium signaling plays critical roles in many biological functions with Ryanodine and IP3 Receptor mediated CICR mechanism at cell molecular level. Calcium spark along with its other appearances evidently performs function with RyR/IP3R-FKBP complex associated calcium signaling from fertilization, via skeletal, cardiac and smooth muscle contraction, to presynaptic terminal neuronal cells. Our data suggested that DmRyR-FKBP12 complex plays an essential role as the regulator of DmRyR through CICR pathway. As absence of FKBP12 directly causes early newborn lethality with cerebral edema and severe arrhythmic defect representative with conduction error of cardiomyocyte in vertebrate, our data approved the present of DmFKBP12 in Drosophila early embryonic development and investigated the dynamic distribution of DmFKBP12 protein. The DmFKBP12 subsurface distribution apposition to the plasma membrane and cortex distribution within the multinuclear plasma directly supports the critical role performed through DmRyR-FKBP12 complex in Drosophila melanogaster embryo as early as at the stages of syncytial blastoderm, via cellular blastoderm and to late gastrula. Our data propose a unique potential to develop insect specific and long-term (from early embryo and adult) effective pesticide by targeting on insect embryonic RyR-FKBP12 complex against insect CICR calcium signaling pathway.