The lysine methyltransferase Ehmt2/G9a is dispensable for skeletal muscle development and regeneration

Euchromatic histone-lysine N-methyltransferase 2 (G9a/Ehmt2) is the main enzyme responsible for the apposition of H3K9 di-methylation on histones. Due to its dual role as an epigenetic regulator and in the regulation of non-histone proteins through direct methylation, G9a has been implicated in a number of biological processes relevant to cell fate control. Recent reports employing in vitro cell lines indicate that Ehmt2 methylates MyoD to repress its transcriptional activity and therefore its ability to induce differentiation of activated myogenic cells. To further investigate the importance of G9a in modulating myogenic regeneration in vivo, we crossed Ehmt2floxed mice to animals expressing Cre recombinase from the Myod locus, resulting in efficient knockout in the entire skeletal muscle lineage (Ehmt2ΔmyoD). Surprisingly, despite a dramatic drop in the global levels of H3K9me2, knockout animals did not show any developmental phenotype in muscle size and appearance. Consistent with this finding, purified Ehmt2ΔmyoD satellite cells had rates of activation and proliferation similar to wild-type controls. When induced to differentiate in vitro, Ehmt2 knockout cells differentiated with kinetics similar to those of control cells and demonstrated normal capacity to form myotubes. After acute muscle injury, knockout mice regenerated as efficiently as wildtype. To exclude possible compensatory mechanisms elicited by the loss of G9a during development, we restricted the knockout within adult satellite cells by crossing Ehmt2floxed mice to Pax7CreERT2 and also found normal muscle regeneration capacity. Thus, Ehmt2 and H3K9me2 do not play significant roles in skeletal muscle development and regeneration in vivo.


Background
The formation of skeletal muscle begins in the embryonic somites [1], which generate the primary myotome and the first primitive myogenic structure containing muscle progenitors. Morphogen gradients including sonic hedgehog (Shh) [2,3] and Wingless (Wnt) [4] ensure initial myogenic specification by controlling the expression of myogenic regulatory factors (MRFs-Myf5, Myod, Myog, and Mrf4)-a conserved family of musclespecific basic helix-loop-helix (bHLH) transcription factors responsible for myogenic lineage commitment and differentiation. Embryonic muscle progenitors migrate, expand, and undergo subsequent waves of myogenesis persisting through fetal and early neonatal development resulting in the formation of the different skeletal muscles of the adult.
A proportion of fetal myoblasts also become localized underneath the basal lamina of newly formed myofibers. These cells become specified as satellite cells-the quiescent, tissue resident stem cell of the skeletal muscle, identifiable by the expression of paired box transcription factor, paired box 7 (Pax7). Satellite cells are responsible for the regenerative potential of the muscle and, upon acute injury, break quiescence and mimic their developmental programs by expanding rapidly, upregulating MRFs and differentiating to form new myofibers. In mice, this process leads to near-complete tissue regeneration and restoration of muscle function.
Despite much knowledge about the key transcription factors regulating myogenesis, the epigenetic landscapes required for the control of gene expression in skeletal muscle differentiation remain less well understood. Epigenetic mechanisms including different modifications on the unstructured C-terminals (tails) of histone proteins have an increasingly appreciated role in controlling gene expression during myogenesis [5]. Some studies [6][7][8] have found that specific histone acetyltransferases can interact with Myod and acetylate histones associated with muscle-specific genes, thereby activating their transcription. Recent genome-wide analyses have uncovered dynamic epigenetic changes during myogenesis [9], including the loss of histone 2B (H2B) ubiquitination [10]. Chromatin immunoprecipitation (ChIP)-seq analyses have also revealed the importance of bivalent domains containing both H3K4me1 and H3K27ac in regulating muscle enhancers during myogenesis [9]. These data also point to Myod as playing a key role in the recruitment of chromatin-modifying enzymes and transcription factors to activate such enhancers [11,12].
A less well-characterized histone mark in myogenesis is H3K9me2, produced by euchromatic histone-lysine Nmethyltransferase 2 (Ehmt2) (MGI: 2148922), also known as G9a. This Su (var)3-9 and enhancer of zeste (SET) domain containing methyltransferase dimerizes with its close homologue Ehmt1 (aka Glp) to induce H3K9me2 [13]. Knockout of Ehmt2 leads to a global reduction of H3K9me2 levels and early embryonic lethality in mice, underscoring its importance and the fact that Ehmt1 cannot fully compensate for its loss [14]. Conditional knockouts of Ehmt2 also demonstrated its importance in germ cell development [15], heart development [16], lymphocyte development [17,18], leukemia [19], drug addiction [20], cognition, and adaptive behaviors [21,22]. H3K9me2 appears to occur on repressed genes in euchromatin, whereas H3K9me3 is also a repressive mark, but associated with pericentromeric heterochromatin [23]. The repressive function is led by recruitment of proteins such as HP1, which bind preferentially to H3K9me2 and establish a repressive chromatin conformation [15]. The SET domain in Ehmt2 and other histone methyltransferases also have the potential to methylate non-histone polypeptides [24,25], allowing possible regulation of the localization and activity of a variety on non-histone proteins [26]. Ehmt2 contains an ankyrin domain, enabling protein-protein interactions [27] with a variety of partners including DNA methyltransferases (DNMT) [28], which provide an alternative mechanism for gene repression [29]. Finally, Ehmt2 can act as a gene activator in addition to its repressive roles, by interacting with coactivators such as in nuclear receptor-mediated transcription regulation [23,30].
Despite Ehmt2 being capable of regulating a diverse range of cellular and biological functions, little is known about its role in skeletal muscle. Working with the widely used murine cell line C2C12, Ling et al. [31] recently highlighted Ehmt2 as a possible regulator of myogenic differentiation. Using in vitro overexpression and knockdown strategies, Ehmt2 was shown to act as an inhibitor of myotube formation. Biochemical analyses suggested that EHMT2 also has the capability to directly methylate MYOD at K104 [31], revealing a novel Bhlhe41/Sharp1-dependent mechanism inhibiting myogenesis that is controlled by Ehmt2 through both direct modulation of MYOD and the repressive H3K9me2 modification of Myod target genes [32,33]. In spite of these initial findings, whether Ehmt2 plays an equivalent role in a more complex biological system such as in vivo skeletal muscle development or regeneration is yet to be evaluated. In this study, we generated transgenic mouse strains to genetically delete Ehmt2 during muscle development as well as in adult satellite cells. We found that proliferation and differentiation of satellite cells was not influenced by the absence of Ehmt2. Knocking out Ehmt2 also failed to result in significant consequences for skeletal muscle development and in adult muscle regeneration in vivo. Thus, Ehmt2 is completely dispensable for the normal functioning, maintenance, and damage response of murine skeletal muscle.

Methods
Mice and animal care C57BL/6 mice harboring the Ehmt2 floxed allele were previously generated by Lehnertz et al. [17]. In this strain, Cre-mediated targeting of the Ehmt2 floxed allele results in a genomic deletion from exon 4 to exon 20 and a frameshift mutation that places the downstream coding sequence out of frame. All other transgenic strains used herein were generated by other groups [34][35][36] and obtained from The Jackson Laboratory. Inducible Cre recombinase was activated by intraperitoneal injection of tamoxifen dissolved in corn oil (250 mg/kg of body weight per day) for 5 consecutive days, followed by 7 days without any treatment to allow sufficient activation. The mice were housed in a pathogen-free facility, and all experiments were performed according to the Canadian Council on Animal Care (CCAC) regulations.
Analysis was performed on LSRII (Becton Dickinson) equipped with three lasers. Data were collected using FACSDiva software. Sorts were performed on a FACS Influx (Becton Dickinson) or FACSAria (Becton Dickinson), both equipped with three lasers, using a 100-μm nozzle at 18 psi to minimize the effects of pressure on the cells. Sorting gates were strictly defined based on "fluorescence minus one" stains.

Cell culture and immunocytochemistry
Viable single myofibers were isolated from the extensor digitorum longus (EDL) muscle of 6-8-week-old mice following dissociation with collagenase I as previously described [38]. Myofibers and their associated satellite cells were maintained ex vivo for up to 72 h in highglucose Dulbecco's modified Eagle's medium (DMEM) supplemented with 20 % v/v FBS, 0.5 % v/v chick embryo extract, and pen-strep. Following culture, single myofibers were fixed in 4 % PFA and then stained overnight with the following primary antibodies: mouse anti-Pax7 (Developmental Studies Hybridoma Bank (DSHB)), mouse anti-MyoD (Dako, clone 5.8A), mouse anti-Myogenin (DSHB, clone FD5).
Fluorescence-activated cell sorting (FACS)-sorted cells were grown in high-glucose DMEM, supplemented with 2.5 ng/ml bFGF (Invitrogen) 20 % v/v FBS, and 10 % v/v horse serum. This medium is hereafter referred to as "growth medium." Cells were seeded in tissue culturetreated plastics coated with Matrigel (BD Biosciences).
The media were changed every 24-48 h. To induce myogenic differentiation, confluent myoblasts were cultured in DMEM supplemented with 5 % horse serum for up to 96 h, before being fixed in 4 % PFA and stained overnight with mouse anti-Myosin (DSHB, clone MF20).

Histology
TA muscles were dissected from mice, fixed in 4 % paraformaldehyde overnight followed by 70 % ethanol overnight, and then embedded in paraffin following standard protocols. Tissues were cut with a microtome in a crosssectional orientation through the entire length of the muscle. Cross sections of 5 mm thickness were then mounted onto glass slides (Thermo Fisher Scientific, USA) and stained with Masson's trichrome or Picrosirius red following standard protocols. The cross-sectional area was used as a measure of myofiber size, which was produced by semi-automated measurements on stitched whole-section images (Nikon).

Gene deletion efficiency measured by allele count
At least 10,000 FACS-purified satellite cells per sample were used for measuring the efficiency of Ehmt2 conditional knockouts. Cells were lysed and purified for genomic DNA, of which 50 ng per sample was mixed with digital droplet PCR supermix (Bio-Rad) and two TaqMan Copy Number Assays for a duplex (fluorescein amidite (FAM) and VIC) digital droplet PCR assay. The "functional assay" is a TaqMan Copy Number Assay with FAM dye (Thermo Fisher Scientific #4400291 Mm00466045_cn) that detects Ehmt2 in a region from intron 14 to exon 15, which is found only in the wildtype or floxed alleles (functional alleles) of the gene. The "reference assay" is a TaqMan Copy Number Assay with VIC dye (Thermo Fisher Scientific #4400291 Mm00466690_cn) that detects Ehmt2 in a region from intron 25 to intron 26, which is found in any null, wildtype, or floxed alleles of the gene. Droplets of the mixture were generated according to standard digital droplet PCR protocol (Bio-Rad) and ran in a thermocycler for 40 PCR cycles. PCR products were read droplet-wise in duplex (FAM and VIC) following standard protocol (Bio-Rad), and a signal ratio was calculated by dividing the absolute copy number of the functional assay to the copy number of the reference assay. The signal ratio was then used to interpolate the functional allele frequency (%) from a known standard curve (see Additional file 1: Figure S1). The standard curve of signal ratios was produced by performing digital droplet polymerase chain reaction (ddPCR) using genomic DNA that were mixed at known proportions from Ehmt2 wt/wt and Ehmt2 null/null mouse embryonic fibroblasts. The standard curve has a Pearson coefficient of 0.993, and a statistical test of linearity yielded a p value of 0.0001. The results of this measurement are presented either as the frequency of functional alleles in each experimental sample or as the efficiency of gene deletion, which is calculated as the reciprocal of the functional allele frequency.

Statistics
Mouse weight measurements plotted against age were subjected to linear regression analysis. A sum-of-squares F test was performed on a shared model to test the null hypothesis that one curve fits all groups. The resulting p value was used to conclude the differences in growth pattern between the groups. Error for mean of means is propagated by weighted pooled variance.

Ehmt2 (G9a) is dispensable in skeletal muscle development
To examine the role of Ehmt2 in myogenesis in vivo, we first established a transgenic mouse model in which the Ehmt2 gene was conditionally knocked out in the skeletal muscle lineage. Mice harboring loxP-flanked Ehmt2 alleles (Ehmt2 floxed ) were crossed to mice with a Cre recombinase gene knocked in to the Myod locus (Myod Cre ) [34]. To verify the efficiency of the conditional knockout, we performed an exon-specific allele-counting assay using digital droplet PCR to measure functional allele frequency [19] (see the "Methods" section for details). In FACS-purified satellite cells from control mice (Myod wt/wt Ehmt2 floxed/floxed ), the Ehmt2 functional allele frequency was 100 %; whereas in the knockout mice (Myod wt/Cre Ehmt2 floxed/floxed ), the functional allele frequency was reduced to 2.9-7.9 % (95 % CI) (Fig. 1a). These genomic results were consistent with immunostaining quantification of EHMT2 protein in satellite cells. In wildtype mice, EHMT2 was robustly expressed in activated satellite cells whereas no detectable staining was present in the knockout mice (Fig. 1c). Furthermore, western blot analysis of whole skeletal muscle lysates from the conditional knockout mice showed reduction of H3K9me2 levels compared to those of the wildtype (Fig. 1b), congruent with previous reports that H3K9me2 is diminished in Ehmt2 null/null models [14].
Knockout and control group progenies from Myod Cre and Ehmt2 floxed breeding were born at expected Mendelian frequencies (Fig. 1d), and neonatal weights were similar between both groups (Fig. 1e). Growth patterns of knockout and control group progenies were charted by body weight, which showed no statistically significant differences (Fig. 1f). Mature skeletal muscles in these mice are of similar size (Fig. 1g)  myofiber size by measuring the cross-sectional area of myofibers (Fig. 1h, i). These findings indicate that Ehmt2 in the skeletal muscle lineage is dispensable in embryonic and fetal development.
Ehmt2 (G9a) knockout satellite cells have normal proliferation kinetics and differentiation capacity in vitro As previous reports have suggested that Ehmt2 is an important regulator of C2C12 myogenesis, we next assessed whether it plays a similar role in satellite cell and primary myoblast cultures ex vivo. To analyze satellite cell proliferation, we performed a 4-h 5-ethynyl-2′-deoxyuridine (EdU) pulse on myofiber-associated satellite cells from the wildtype and conditional knockout mice after 72 h in culture.
No differences in EdU incorporation were detected between the control and knockout groups (Fig. 2a, e). Similarly, quantification of immunofluorescent staining showed similar numbers of PAX7+ (Fig. 2c) and MYOD+ (Fig. 2b) satellite cells after 72 h in culture. These results indicate that satellite cells lacking Ehmt2 show comparable rates of proliferation and myogenic activation to wild-type cells ex vivo. Next, we assessed the requirement of Ehmt2 for satellite cells to undergo myogenic differentiation. Satellite cell-derived myofibers from wildtype (WT) and knockout (KO) mice were expanded to confluence, induced to differentiate, and then analyzed by immunostaining of MYOG and myosin heavy chain after 4, 24, and 48 h. Under differentiating conditions, MYOG-expressing cells increased, but no significant differences in the percentages of cells expressing MYOG were observed between control and KO myoblasts (Fig. 2e). Similarly, we found no significant differences in myogenic fusion index (ratio of fused nuclei found in myosin-expressing cells to total nuclei) following 48 h of differentiation (Fig. 2f), providing further support that deletion of Ehmt2 does not have significant effects on the progress or timing of myogenic differentiation in primary myoblasts.
Together, these data provide little evidence that Ehmt2 plays a major role in the regulation of satellite cell proliferation or myogenic differentiation in vitro.

Skeletal muscle-and satellite cell-specific deletion of Ehmt2 (G9a) has little effect on muscle regeneration in vivo
Before evaluating the requirement of Ehmt2 in the response of skeletal muscle to acute injury in vivo, we first analyzed the expression of the gene in our injury model in WT mice, which involved an intramuscular injection of notexin (a snake venom toxin) in the TA muscle. During the ensuing regenerative process, we performed transcriptome sequencing of WT primary myoblasts at Myoblasts were seeded at equal densities and induced with differentiation media for 4, 24, and 48 h. d, e Immunofluorescence detection of EHMT2 and MYOG, respectively. f Immunofluorescence detection of myosin heavy chain in myotubes, myoblast fusion index calculated as % nuclei inside myosin-expressing myotubes different timepoints; in our results, neither Ehmt2 nor Ehmt1 showed any dynamic changes in expression during the process, in contrast to key myogenic regulators (Additional file 1: Figure S2).
Then, we evaluated the aforementioned Myod Cre Ehmt2 floxed/floxed model, which deletes Ehmt2 in all skeletal muscles and satellite cells during their development [39]. Following injury, we quantified the cross-sectional area of centrally nucleated myofibers as a measure of regeneration [40]. Despite a trend toward an increased number of the largest fibers in KO samples, no statistically significant difference was found between the control and KO mice in the distribution of myofiber size at 7, 14, or 21 days post injury, indicating comparable regenerative capacities (Fig. 3b, Additional file 1: Figure S3).
Myod-driven CRE leads to target deletion early in development and could therefore trigger compensatory effects that mask the regulatory role of Ehmt2 in adult regenerative myogenesis. To mitigate this risk, we performed a satellite cell-specific, inducible Ehmt2 KO using a strain carrying the tamoxifen-activated CreERT2 recombinase knocked in to the Pax7 locus [35]. This allowed us to confine the gene deletion within adult satellite cells and to use a Rosa26 YFP reporter to monitor the efficiency of induction. One week after the end of tamoxifen treatment, we found that the YFP reporter was activated in 70 % of cells in the satellite cell population (Additional file 1: Figure S4). Since this was not a definitive measure of gene deletion, we further performed Ehmt2 allele count and found that the functional allele frequency had been reduced to 27.2 ± 4.2 % (SEM) in the satellite cell population purified by FACS.
Acute muscle injury by notexin was performed at 7 days after the final CreERT2 induction on mice harboring Pax7 CreERT2 and Ehmt2 floxed/null alleles. At 7, 14, and 21 days after the injury, no significant differences in myofiber size distribution were observed between the control and KO groups (Fig. 3f, Additional file 1: Figure  S5, data not shown), suggesting that lack of Ehmt2 in adult satellite cells does not significantly affect repair and regeneration in vivo.
Together, our data provide little evidence for a role of Ehmt2 in regulating skeletal muscle development, homeostasis, or regeneration in vivo.

Discussion
In this study, we provide a comprehensive assessment of the biological consequences of G9a deletion in skeletal muscle progenitors. Our data strongly suggests that Ehmt2 is dispensable for both developmental and regenerative myogenesis in vivo and is not required for normal satellite cell proliferation and myogenic differentiation in vitro.
Although germ line deletion of Ehmt2 is embryonically lethal, conditional KO models suggest that Ehmt2 plays distinct roles in different tissues. Studies have shown an involvement of Emht2 in the regulation of embryogenesis [14,28,41], cardiac morphogenesis [16], lymphopoiesis [17,18], myelopoiesis [19], germ cell development [15], brain and cognitive development [21,22], and drug addiction [20], confirming Ehmt2 is capable of controlling a diverse range of biological processes. In the case of myogenesis, we showed that conditional loss of Ehmt2 in vivo does not induce any significant developmental impact or any significant alterations to the regenerative capacity of myogenic progenitors in response to skeletal muscle injury. Deletion of Ehmt2 in primary myoblasts also fails to induce any significant alterations in proliferative kinetics or differentiation capacity in vitro, suggesting little role for Ehmt2 in regulating myogenesis.
These results are surprising given the previous reports suggesting an important role for Ehmt2 in negatively regulating myogenic differentiation of C2C12 cells, an immortalized myogenic line. In particular, it was demonstrated that siRNA knockdown of Ehmt2 led to enhanced and/or premature differentiation of C2C12 myoblasts [31]. It was further reported that Ehmt2 is an integral component of the mechanism with which Bhlhe41/Sharp1 regulates in myogenesis, in that it is recruited by SUMOylated BHLHE41/SHARP1 [33], methylates MYOD at lysine 104 [31], and leads to repressive H3K9me2 modifications on Myod targets [32]. These results were not consistent with observations in the current study when examining the differentiation capacity of primary myoblasts lacking Ehmt2, which showed no premature differentiation, no alterations in myogenic fusion, and normal proliferation. This discrepancy in findings could stem from the fundamental differences between the biological models being analyzed; unlike C2C12 cells, which were derived from a different strain of mouse (C3H) [42], immortalized [43], and have a much shorter doubling time [44], the primary myoblasts in our study were not serially passaged and were analyzed in their myofiber niche during proliferation and on Matrigel during differentiation, in addition to in vivo analyses. These differences may be particularly relevant in the case of Ehmt2, as we have recently reported, in another tissue system, that its absence has drastically different effects on transformed compared to natural hematopoietic cells [19].
To date, no genome-wide analysis of the Ehmt2-mediated H3K9me2 in myogenic cells exists. Dynamic changes in H3K9me2 have been reported at specific gene bodies and regulatory regions [45,46], suggesting that modulating this epigenetic mark may affect gene transcription. However, in our experiments, lack of Ehmt2 led to a dramatic drop in the global levels of detectable H3K9me2 in the absence of any effects on skeletal muscle development. This indicates that Ehmt2 activity is mostly non-redundant and questions the importance of Ehmt2-mediated histone modifications in myogenesis in particular and in the control of differentiation in general. What remains unclear is the status of EHMT2mediated methylation of MYOD at lysine 104 [31] in the in vivo model. Ling et al. reported the identification of this residue by mass spectrometry of peptides resulting from the digestion of MYOD with trypsin [31]. However, the reported MYOD peptides, ACKACKRKTT and its methylated forms, do not appear to be obtainable by trypsin digestion alone or by any commonly used digestion method. The reported MYOD peptide is also not found in the tandem mass spectrometry proteomics repository PeptideAtlas (https://db.systemsbiology.net/ sbeams/cgi/shortURL?key=1mk36ybs). More intriguingly, the proposed mechanism [31] was based on liquid chromatography-mass spectrometry (LC-MS) results, showing that the different methylation states of the MYOD peptide are separated by only 1 m/z unit each. Methylation adds 14 Da to the peptide mass; thus, each peptide would have to carry 14 charges on 10 residues. The LC-MS results could not possibly correspond to methylation of the reported MYOD peptide. The uncertainty of MYOD methylation, together with the dispensability of Ehmt2-mediated H3K9me2 in vivo, casts doubts on the proposed role of Ehmt2 in Bhlhe41/Sharp1-mediated regulation of myogenesis [32]. Nevertheless, SUMOylated Bhlhe41/Sharp1 [33] may still regulate Myod and downstream targets through an alternative mechanism.
Although we have shown here that the loss of EHMT2's histone methylation function in our model was not compensated, the possibility exists that its potential interaction with myogenic regulators could be compensated by another gene, such as its close homologue Ehmt1 (GLP). These two genes are highly similar in structure, as their protein products contain highly similar catalytic domains for lysine methylation (SET domain) and a set of ankyrin repeats for protein-protein interaction. These two enzymes are known to form heterodimers [14] but also play unique roles depending on the cell type and developmental stage [47]. Using domain-specific mutations, the EHMT1 but not the EHMT2 ankyrin repeats were found to be required for mouse viability [48], suggesting that this domain function in EHMT2 could be compensated by EHMT1. On the other hand, the SET domain in EHMT1 is dispensable for mouse viability [16], suggesting EHMT2 could compensate for EHMT1's methyltransferase activity. In our Ehmt2 KO cells, even though the histone methyltransferase activity, a function shared by both proteins, is not compensated by EHMT1, its ankyrin repeatdependent protein-protein interactions may be compensated. Both proteins have been reported to methylate a number of non-histone targets beyond MYOD [49,50], and it is unknown if EHMT1 could replace EHMT2 in binding and methylating these targets. Interestingly, such compensation by Ehmt1 was not observed in C2C12 studies [31], and in our transcriptome data, neither of the genes showed any dynamic changes during regeneration. Nevertheless, to fully address this concern, a conditional double KO of Ehmt2 and Ehmt1 in myogenesis would be required.

Conclusions
In this study, we analyzed tissue-specific KO models of Ehmt2 both during development and in adult satellite cells, and unlike as previously reported, we found no evidence for a significant role of this methyltransferase in the development or regeneration of skeletal muscle. The drop in H3K9me2 levels observed in cells lacking Ehmt2 strongly suggests that this histone modification is dispensable for the regulation of myogenesis. Primary cultures revealed that Ehmt2 does not significantly alter the proliferation and differentiation processes of satellite cells. Thus, the proposed regulatory role of Ehmt2 in myogenesis cannot be validated in vivo.    Fig. 3 Ehmt2 is dispensable for muscle regeneration. a Schematic diagram of leg injury timeline for Myod Cre Ehmt2 floxed/floxed mice. b, c Masson's trichrome stain of histological sections of the tibialis anterior muscle of adult Myod Cre Ehmt2 floxed/floxed mice at 21 days after injury and myofiber size measurement by cross-sectional area, respectively. d Schematic diagram of leg injury timeline for Pax7 CreERT2 Ehmt2 floxed/null mice. e Ehmt2 deletion efficiency by Pax7 CreERT2 as measured by functional allele frequency in FACS-purified satellite cells. Analysis by gDNA allele counting using ddPCR, n ≥ 3. f, g Myofiber size measurement by cross-sectional area and Masson's trichrome stain of histological sections of the tibialis anterior muscle of induced adult Pax7 CreERT2 Ehmt2 floxed/floxed mice at 21 days after injury, respectively protein); Ehmt2: euchromatic histone-lysine N-methyltransferase 2, aka G9a; FACS: fluorescence-activated cell sorting; FAM: fluorescein amidite; FITC: fluorescein isothiocyanate; H2B: histone 2B; H3K27ac: acetylation of lysine 27 on histone H3 protein subunit; H3K4me1: unimethylation of lysine 4 on histone H3 protein subunit; H3K9me2: trimethylation of lysine 9 on histone H3 protein subunit; HP1: heterochromatin protein 1; KO: knockout; LC-MS: liquid chromatography-mass spectrometry; LY6A/E: lymphocyte antigen 6A/E, aka SCA-1 (stem cell antigen 1); MRF: myogenic regulatory factor; Mrf4: myogenic regulatory factor 4, aka myogenic factor 6 (herculin); Myf5: myogenic factor 5; Myog: myogenin; Pax7: paired box 7; PE: phycoerythrin; PECAM: platelet endothelial cell adhesion molecule, aka CD31 (cluster of differentiation 31); PE-Cy7: phycoerythrin-cyanine 7 tandem complex; PTPRC: protein tyrosine phosphatase receptor type C, aka CD45 (cluster of differentiation 45); SEM: standard error of mean; SET: Su (var)3-9 and enhancer of zeste; Shh: sonic hedgehog; SUMO: small ubiquitin-like modifier; TA: tibialis anterior; VCAM: vascular cell adhesion molecule, aka CD106 (cluster of differentiation 106); WT: wildtype.