Sestrin2 is involved in asthma: a case–control study

Background Asthma is a chronic disease that seriously harms the health of patients. Oxidative stress is involved in asthma. As an oxidative stress-inducible protein, sestrin2 is elevated in oxidative stress-related diseases. We aimed to explore whether sestrin2 was involved in asthma. Methods Seventy-six subjects (44 in the asthma group, 32 in the control group) were recruited in this study. Plasma sestrin2 levels, peak expiratory flow (PEF), forced expiratory volume in 1 s (FEV1) % predicted, forced vital capacity (FVC) % predicted and FEV1/FVC ratio were measured in controls and in asthmatics both during an exacerbation and when controlled after the exacerbation. Results The asthma group had a significant higher sestrin2 level than the control group (asthmatics during exacerbation, 1.75 ± 0.53 ng/mL vs. 1.32 ± 0.48 ng/mL, p < 0.001; asthmatics when controlled after the exacerbation, 1.56 ± 0.46 ng/mL vs. 1.32 ± 0.48 ng/mL, p = 0.021, respectively). In addition, sestrin2 was negatively correlated with FEV1% predicted and FEV1/FVC ratio in asthmatics during exacerbation (r = − 0.393, p = 0.008; r = − 0.379, p = 0.011; respectively). Moreover, negative correlations between sestrin2 and FEV1% predicted and FEV1/FVC ratio also existed in asthmatics when controlled after the exacerbation (r = − 0.543, p < 0.001; r = − 0.433, p = 0.003 respectively). More importantly, multiple linear regression analysis demonstrated that FEV1% predicted was independently associated with sestrin2 in asthmatics both during exacerbation and when controlled after the exacerbation. Conclusions Sestrin2 is involved in asthma. Sestrin2 levels increase in asthmatics both during exacerbation and when controlled after the exacerbation. In addition, sestrin2 is independently associated with FEV1% predicted.


Background
Asthma is a common disease clinically characterized by recurrent wheeze, shortness of breath, chest tightness and cough [1]. Currently, 235-334 million people worldwide suffer from this disease which seriously endangers their health and even causes death [2,3]. According to WHO, the estimated death due to asthma in 2015 was 383,000 [2]. Asthma is usually characterized by chronic airway inflammation which leads to airway remodeling and fixed airflow limitation followed by poor asthma control [1]. Accordingly, airway inflammation is one of the most important targets of asthma treatment. Currently, the diagnosis and treatment of asthma are usually based on the pattern of symptoms in combination with lung function. However, symptoms and lung function, to some extent, may not fully reflect the level of inflammation [4]. Biomarkers can partly reflect the level of airway inflammation [5]. In addition, biomarkers may provide assistance for diagnosis, targeted treatment and monitoring of asthma [6]. Recently, several biomarkers have been reported, including immunoglobulin E (IgE), eosinophil, neutrophil, periostin, dipeptidyl peptidase 4, protein YKL-40, cluster of differentiation 93 (CD93), etc. [4,[7][8][9].
It was reported that oxidative stress increased in asthma and caused airway inflammation and airway remodeling [16,17]. Therefore, we speculated that sestrin2 might also be involved in asthma. The aim of this study was to explore whether sestrin2 was involved in asthma to set the stage for future study.

Subjects
All 76 subjects were recruited from the First Affiliated Hospital of Kunming Medical University. 44 patients were from the outpatient service or hospital admission for asthma exacerbation treatment, and 32 healthy subjects were from the Health Examination Center of hospital. Comprehensive physical examination, laboratory test and medical history collection were performed for all patients prior to enrollment.
Inclusion criteria: (1) Patients visited a doctor for asthma exacerbation; Exacerbations of asthma are episodes characterized by progressive increase in symptoms of shortness of breath, cough, wheezing or chest tightness and progressive decrease in lung function, which means the current treatment needs to be changed to meet the patient's requirements according to the Global Strategy for Asthma Management and Prevention (GINA) guidelines [1]. (2) Patients who had been diagnosed with asthma prior to enrollment according to clinical history of symptoms with airflow limitation and positive bronchial provocation or bronchodilatation test. Positive bronchodilatation was defined that the FEV 1 increased > 12% and 200 ml from baseline, 10-15 min after 200-400 mcg albuterol being inhaled, and positive provocation was defined that FEV 1 decreased by 15% of baseline with hypertonic saline challenge according to the GINA guidelines [1]. (3) The lung function data [peak expiratory flow (PEF), forced expiratory volume in 1 s (FEV 1 ) % predicted, forced vital capacity (FVC) % predicted and FEV 1 /FVC ratio] were available at exacerbation and relief from exacerbation with treatment.
This study was approved by the Ethics Committee of Hospital, and informed consent was obtained before the study.

Treatment of asthma exacerbation
The treatments were divided into three levels. First level: patients were treated with inhaled salbutamol or budesonide/formoterol at the time of first arrival to the outpatient service or admission. Second level: the efficacy of the first level treatment was evaluated 1-2 h later. If the patient's symptoms didn't improve, the doxofylline accompanied with inhaled budesonide and terbutaline were added. Third level: the efficacy of the second level treatment was evaluated 4-6 h later, and oral or intravenous methylprednisolone and montelukast would be used on the basis of second level treatment if the patient's symptoms still didn't improve. The patients who couldn't be improved with treatment of the three levels or patients who needed mechanical ventilation were excluded from this study.

Relief of asthma exacerbation
At any level of the treatment above, if the patient's symptoms were improved, the treatment would be maintained until the patient's symptoms disappeared. Then the treatment of asthma exacerbation was completed and the patients were discharged. The subsequent treatment was changed to inhaled corticosteroids (ICS)/ long-acting beta2-agonist (LABA). In the following 4 weeks of discharge, the patients who met with the GINA criteria for well or partly controlled asthma were included in this study [1]. The patients whose asthma was uncontrolled were excluded from this study.

Blood test
Venous blood samples were obtained at two time spots: first arrival to the outpatient service before treatment or at the time of admission before treatment; at the end of the treatment in hospital. Following a centrifugation at 3000 rpm for 20 min, the supernatant was taken and stored at − 80 °C for testing. Plasma sestrin2 concentrations were detected using an ELISA kit (Mlbio, China). The lung function test was completed by the laboratory of First Affiliated Hospital of Kunming Medical University.

Statistical analysis
The data were expressed as mean ± standard deviation, and the one-sample Kolmogorov-Smirnova method was used to detect whether the data were normally distributed. Normally distributed data were compared using an unpaired t test. Paired t test was used in comparison between before and after treatment, and rank sum test was used for non-normal distribution data. Spearson's correlation analysis was used to test the correlations between sestrin2 and age, gender, body mass index (BMI), smoking index, PEF, FEV 1 % predicted, FVC% predicted, and FEV 1 /FVC ratio. Multiple linear regression analysis with stepwise selection was used to determine the relationships between sestrin2 and age, BMI, smoking index, FEV 1 % predicted, PEF, FVC% predicted, and FEV 1 /FVC ratio. Statistical analyses were performed with SPSS17.0.

Results
Seventy-six subjects were enrolled in the study and divided into asthma group (n = 44) and control group (n = 32). The demographic characteristics, results of lung function and sestrin2 test were listed in Table 1.
Comparisons between sestrin2 concentrations in control group and in asthma group (during exacerbation and when controlled after the exacerbation) were presented in Fig. 1. Both during exacerbation and when controlled after the exacerbation, the asthma group had Correlations between sestrin2 and lung function parameters were listed in Table 2. The level of sestrin2 was negatively correlated with PEF, FEV 1 % predicted and FEV 1 /FVC ratio in asthmatics during exacerbation. Moreover, negative correlations between sestrin2 and FEV 1 % predicted and FEV 1 /FVC ratio also existed in asthmatics when asthma was controlled after the exacerbation ( Table 2).
The multiple linear regression analysis with stepwise selection demonstrated that sestrin2 was independently associated with FEV 1 % predicted in asthmatics both during exacerbation and when controlled after the exacerbation (Table 3).

Discussion
The present study demonstrated that the level of sestrin2 in patients with asthma was significantly higher than that of the healthy subjects. In addition, sestrin2 was negatively correlated with FEV 1 % predicted and FEV 1 / FVC ratio in asthmatics both during exacerbation and when controlled after the exacerbation. More importantly, multiple linear regression analysis showed that sestrin2 was independently associated with FEV 1 % predicted in asthmatics both during exacerbation and when controlled after the exacerbation.
It was reported that oxidative stress was involved in the pathogenesis of asthma [16,17]. Airway oxidative stress results from a variety of causes, including excessive exposure to environmental pro-oxidants, airway infiltration of inflammatory cells, metabolic disorders, and decreased levels of antioxidants [18,19]. Oxidative stress may be the result of inflammation and may also trigger and increase inflammation [17]. Sestrin2 has been found as a stress-responsive protein which was strong associated with excessive oxidative stress, hypoxia, and DNA damage [20]. In addition, hypoxia causes up-regulation of sestrin2 [10,21,22]. Hypoxia stimulates the mitochondria and increases ROS, results in hypoxic stress, and induces elevated level  of sestrin2 [11]. When asthma is exacerbated, airflow is significantly limited, resulting in increased hypoxia, leading to oxidative stress [23]. When asthma is controlled, oxidative stress tends to decrease and may still exist [24]. Therefore, we speculated that the increase in sestrin2 of asthma patients might be related to the aggravation of hypoxia caused by airflow limitation. FEV 1 % predicted is an important indicator of airway function, and the more FEV 1 % predicted decrease, the more severe the asthma is [1]. In this study, sestrin2 was negatively correlated with FEV 1 % predicted, and multiple regression analysis showed that sestrin2 was independently and negatively associated with FEV 1 % predicted, which meant that sestrin2 might be helpful to assess the FEV 1 % predicted and accordingly to assess the severity of asthma, especially for the patients whose FEV 1 % predicted could not be obtained because of the inability to perform lung function testing, such as patients who cannot cooperate, etc. It also meant that sestrin2 might be useful for guiding the treatment of asthma. In addition, studies had shown that sestrin2 could up-regulate autophagy through activating AMPK [25,26]. Increasing studies suggested that the level of autophagy in airway smooth muscle cells was significantly increased in asthma [27,28]. Therefore, the role of sestrin2 in asthma needs further researches to confirm.
It was reported that sestrin2 could inhibit the signal transduction of platelet-derived growth factor receptorbeta (PDGFRβ), an upstream regulator in the alveolar damage repair process [15]. Studies on asthma suggested that inhibition of PDGFRβ signaling, in the context of exposure to chronic air allergens, can lead to increased lung dysfunction and thickening of airway smooth muscles, resulting in airflow limitation [29]. The present study shows that sestrin2 is independently associated with FEV 1 % predicted which is an indicator of the severity of airflow limitation. Accordingly, we speculate that sestrin2 might be involved airway remodeling and airflow limitation in asthma.
Some limitations exist in this study. Firstly, this study is not a randomized research and the sample size is small. Secondly, we didn't investigate the effects of related treatments on sestrin2, such as glucocorticoids, β2-adenosine receptor agonists, etc. In addition, another limitation was that time of discharge from hospital might not be the ideal time to capture the well controlled asthma data, though asthma control was assessed within 4 weeks after discharge, which resulted in the inclusion of some partly controlled asthma patients in this study. Nonetheless, our study shows that sestrin2 level significantly increases in asthma, which indicates that sestrin2 is involved in asthma.

Conclusions
Sestrin2 is involved in asthma. Sestrin2 levels increase in asthmatics both during exacerbation and when controlled after the exacerbation. In addition, sestrin2 is independently associated with FEV 1 % predicted.