WNT signaling suppresses oligodendrogenesis via Ngn2-dependent direct inhibition of Olig2 expression

Olig2 transcription factor is essential for the maintenance of neural progenitor cells (NPCs) in the pMN domain and their sequential specification into motor neurons (MNs) and oligodendrocyte precursor cells (OPCs). The expression of Olig2 rapidly declines in newly generated MNs. However, Olig2 expression persists in later-born OPCs and antagonizes the expression of MN-related genes. The mechanism underlying the differential expression of Olig2 in MNs and oligodendrocytes remains unknown. Here, we report that activation of WNT/β-catenin signaling in pMN lineage cells abolished Olig2 expression coupled with a dramatic increase of Ngn2 expression. Luciferase reporter assay showed that Ngn2 inhibited Olig2 promoter activity. Overexpression of Ngn2-EnR transcription repressor blocked the expression of Olig2 in ovo. Our results suggest that down-regulation of WNT-Ngn2 signaling contributes to oligodendrogenesis from the pMN domain and the persistent Olig2 expression in OPCs.

Olig2 is the key transcription factor that not only maintains the neural progenitor cells (NPCs) of pMN domain, but also regulates the sequential specification of NPCs into motor neurons (MNs) and OPCs [1][2][3][4][5]. Since persistent expression of Olig2 is inhibitory to post-mitotic MN genes [6], the expression of Olig2 rapidly declines in newly generated MNs, but remains high in later-born cells of oligodendrocyte lineage [2][3][4][5][6]. The mechanism of down-regulation of Olig2 expression in MNs remains elusive. WNT signaling is known to regulate the balance between the proliferation and differentiation of NPCs during neurogenesis [7]. It is interesting that endogenous WNT/β-catenin signaling is activated in newly generated MNs [8]. Activation of WNT/β-catenin signaling has been reported to inhibit the specification of OPCs and astrocytes from NPCs during early stages of gliogenesis [9][10][11]. However, the mechanism underlying the inhibition of OPC specification from pMN NPCs by WNT/βcatenin signaling remains to be determined.
In line with this concept, two NGN2 recognition sequences are identified in the upstream promoter of the Olig2 gene in human, rat and mouse (Fig. 1b). Luciferase reporter assay revealed that Ngn2 but not its DNA binding defective mutant AQ-Ngn2 can inhibit the promoter activity of mouse Olig2 (Fig. 1c), demonstrating that Ngn2 can bind to the promoter of Olig2 and repress its expression. To confirm that Ngn2 mediates WNT inhibition of oligodendrogenesis, we overexpressed Ngn2 in embryonic chicken spinal cord by in ovo electroporation and found a significant decrease of Olig2 and Pdgfra expression in the electroporated side at cE7 (Additional file 1: Fig. S3). Since Ngn2 can function either as a transcriptional activator or a repressor, we next investigated whether the inhibition of Olig2 expression is mediated by the transcriptional repressor activity of Ngn2. RCAS-Ngn2-EnR (DNA binding domain of NGN2 fused with EnR transcription repressor) was employed as a repressor-only NGN2 chimeric protein. It was found that overexpression of this chimeric repressor caused a significant reduction of Olig2 expression (Fig. 1d), mimicking the effect of full-length Ngn2 protein. This finding demonstrated that Ngn2 inhibits Olig2 expression by its transcriptional repressor activity.
In conclusion, our results suggest that WNT signaling up-regulates the expression of Ngn2, and Ngn2 in turn inhibits Olig2 expression and oligodendrogenesis during MN specification (Fig. 1e).

Additional file 1: Supplementary materials and results.
(See figure on previous page.) Fig. 1 WNT signaling inhibit Olig2 expression through upregulation of Ngn2 expression. a Transverse sections of spinal cord at E12.5 from control and WNT signaling activated (Olig1 Cre/+ ;Ctnnb1 ΔEx3/+ ) mice were subjected to IF with anti-OLIG2 antibody or ISH with Olig1, Pdgfrα and Ngn2 riboprobes. The cells positive for OLIG2, Olig1 and Pdgfrα are absent in the spinal cord from Olig1 Cre/+ ;Ctnnb1 ΔEx3/+ mice, whereas Ngn2 is upregulated. Inset highlights the expression of OLIG2 in pMN domain, note that vascular development was abnormal in the spinal cord of Olig1 Cre/+ ;Ctnnb1 ΔEx3/+ mice. b There are putative Ngn2 binding sequences in the promoter regions of Ngn2 from human, rat and mouse. c Luciferase report assay revealed that Ngn2 but not its DNA binding deficient mutant AQ-Ngn2 inhibit the promoter activity of mouse Olig2. *p < 0.05, t-test. d Over-expression of Ngn2-EnR mimics the phenotype caused by over-expression of Ctnnb1-ΔEx3. Both expression of Ctnnb1-ΔEx3 and Ngn2-EnR suppressed the expression of OLIG2 in ovo. Arrowhead indicates induced expression of chick Ngn2 (cNgn2). Arrows represent reduced expression of endogenous genes. e OLIG2 maintains proliferation of pMN domain neural progenitor cells. High level of WNT signaling upregulates Ngn2 expression, NGN2 in turn coordinate with OLIG2 to promote motor neurons specification and suppress Olig2 expression in newly generated motor neurons. OPCs were specified OLIG2+ cells when WNT signaling is declined at the gliogenesis stage

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