Entomophagy practices, use patterns, and factors influencing perception and consumption frequency of edible insects in the Republic of Benin

Abstract

Background

Edible insects are important sources of essential nutrients and have the potential to contribute to malnutrition reduction and food security in the Republic of Benin. However, their consumption is always restricted to a limited number of sociocultural groups. To determine how the consumption of insects could be promoted as an alternative food source, this study documents the endogenous knowledge associated with edible insects and, the main factors that govern their perception and frequency consumption.

Methods

A survey was conducted towards 479 rural households consuming edible insects through 91 villages of Atacora, Alibori, Zou, and Plateau departments using individual interviews with a semi-structured questionnaire. The survey was focused on the inventory of edible insects and the documentation of consumers’ acceptance, frequencies and motive reason of consumption, local uses, and accessibility to edible insects. Samples of edible insects were collected and preserved in 70% alcohol for taxonomic identification.

Results

The majority of surveyed people (79.1%) were consumers of edible insects since many years ago (29.1 ± 17.2 years). Insect species belonging to 17 genera of 7 families and 3 orders of insects were used as food, with Brachytrupes membranaceus Drury being the most widespread and consumed. Six factors affecting edible insect availability were identified with the chemical pollution as the most important. Besides their food use (63.2%), edible insects in the study area were used for several purposes. We find that ethnicity, religion, age, education level, and monthly frequency of insect consumption are the main factors influencing the local perception of edible insects. Indeed ethnic group, religion ethnicity, and market accessibility have a positive influence on edible insect consumption frequency. The Hierarchical Clustering of Principal Components has allowed us to classify the interviewees into 3 groups with different perceptions of entomophagy and their characteristics will make it possible to better orient the strategies for promoting entomophagy in the Republic of Benin.

Conclusions

Religion and tradition are among the main factors that influence entomophagy in Benin Republic. The development of a national strategy to promote entomophagy should take into account the recorded insect consumption motivations, and their different uses by each ethnic group, and mainly target young people.

Background

Consumed for centuries, edible insects were first promoted as an alternative sustainable source of nutrients for humans and livestock by Meyer-Rochow [1] who recommended that WHO and FAO support the idea. After the report published by the FAO on edible insects [2], some authors supported the assumption that edible insects could provide an alternative source of protein to meet the demand of rapid population growth [3, 4]. Indeed edible insects have been reported to be as rich in proteins, fats, and minerals as traditional meats [1, 5]. Some edible insect species contain more protein than some meat [6], more iron than spinach, as much vitamin B12 as salmon, amino acids, as well as high calcium, Omega 3, and fibre contents [5, 7]. The low environmental pollution, low water usage and land, and fast returns on investment are other benefits of edible insect’s production [8, 9]. Unfortunately, despite all these advantages, edible insects’ acceptability and consumption remain today the biggest challenge for humans and industry [10]. Indeed, several factors from socio–demographic, psychological (insect phobia, feelings of disgust), and cultural influence consumer attitudes and behaviour to accept insects as food and feed [11].

In the Republic of Benin, consumption of edible insects is restricted to a limited number of sociocultural groups, such as the Waama, Dendi, Ottamari, Bariba and Nagot. Although some studies have been conducted on edible insects in Benin, they are mainly limited to their diversity, gathering method, collection place, seasonal availability, consumption patterns, and consumer perception [6, 12,13,14,15,16]. However, little is known about the consumption frequency of edible insects, their variation across different consumption zones in Benin, their traditional uses by Beninese people, and the factors that influence their accessibility. While this information is important not just for the preservation of traditional knowledge associated with edible insects but also for their conservation and sustainable uses.

The development of strategies allowing the acceptance of entomophagy by Beninese populations requires a better understanding of the factors influencing the perception and consumption of edible insects. Indeed, several factors such as consumers’ socio-demographic characteristics, consumers’ external environment, and factors related to the insects consumed are known as influencing the acceptance or rejection of entomophagy [17]. Unfortunately, very few studies have focused on the factors influencing the acceptance and practice of entomophagy by Beninese people. Therefore, no policy is implemented to promote entomophagy despite its potential to reduce malnutrition and food insecurity. Indeed, edible insects, due to their richness in proteins and essential nutrients [18] offer an opportunity to reduce the rate of malnourished children and food insecurity in Benin. Furthermore, to our knowledge, no study has collected data both in the north and in the south of Benin in order to have a general view of the entomophagy practices that are crucial for the implementation of a national policy for the promotion of edible insects. In order to encourage entomophagy in Benin and directly contribute to the attainment of the Sustainable Development Goals (SDGs), it is necessary to carry out investigations on indigenous knowledge related to edible insects and determine factors that promote or hinder the expansion of entomophagy.

The aim of this study was to (1) document the consumption frequency, and local uses of edible insects; (2) examine the local perception of consumers about entomophagy; (3) investigate factors influencing consumers’ perception and consumption of edible insects.

Methodology

Study area

The survey was carried out in 91 villages located across the departments of Atacora (Latitudes/ Longitude: 10° 45′ 0" N/1° 40′ 0" E), Alibori (Latitudes/ Longitude: 11°19′ 0" N/2°55′ 0" E), Zou (Latitudes/ Longitude: 7° 15′ 0" N/2° 10′ 0" E) and Plateau (Latitudes/ Longitude: 7° 10′ 0" N/2° 34′ 60" E) in the Republic of Benin (Fig. 1). These departments are known as the main areas of consumption of edible insects in Benin [6, 12,13,14, 16]. The departments of Atacora and Alibori located in the north of Benin are characterized by a Sudano-Sahelian climate (humid tropical) with a unimodal rainfall regime with a rainy season (April to October) and a dry season (November to March). The average annual rainfall varies from 700 to 1,200 mm with average annual temperatures varying between 26 and 27 °C. This region has a diversity of soils (raw mineral soils, cambisols, tropical ferruginous soils, ferralitic soils, and hydromorphic soils) with woody, shrubby, and grassy vegetation. The departments of Zou and Plateau located in south Benin have a bimodal climate (Sudano-Guinean type) with two rainy seasons and two dry seasons A major rainy season (March to July), a minor dry season in August, a minor rainy season (September to November) and a major dry season (December to March). Annual rainfall varies from 900 to 1300 mm with dominant soils of ferralitic type. The average annual temperature is 28 °C. In the study area, agricultural activities are most dominant, and indigenous knowledge is extensive in terms of the uses of plants and animals for medicinal and cultural purposes [12].

Fig. 1

Map of the Republic of Benin showing the localisation of the surveyed villages

Sample size

The sample size for the study was determined using the formula of Dagnelie [19]:

$$n = \frac{{{\text{Z}}^{2} \times p\left( {1 - p} \right) \times N}}{{Z^{2} \times p\left( {1 - p} \right) + \left( {N - 1} \right) \times ET^{2} }}$$

"n" the required sample size."N" the actual size of the population."ET" the standard error (margin of error) tolerable for the survey: ET = 0.05."z" the normal standard value of the confidence interval: Z ≈ 1.96."p" proportion of rural population in study areas.

The size n of the sample is thus substantially equal to 400 individuals. The number of people interviewed per locality was determined by proportionality considering the size of the population of each locality. However, only people who had eaten edible insects at least once in their life were included in the survey.

Selection of respondents

The respondents of this study were people who had practiced entomophagy in the past and who, today, continue or not to consume edible insects. These respondents were chosen from the villages of the Alibori, Atacora, Zou and Plateau departments, which represent the major regions of consumption of edible insects in the Republic of Benin [6, 12,13,14, 16]. In each department, the surveyed villages were chosen on the basis of previous studies mentioning edible insects carried out in the Republic of Benin [12,13,14, 16]. Within each selected village, the surveyed households practicing entomophagy were chosen with the help of the village chief, and using the snowball method [20]. In total, 479 rural households consuming edible insects were surveyed in the study area (Table 1).

Table 1 Socio-demographic characteristics of respondents by surveyed department

Survey

Data were collected during face-to-face interviews on the basis of a pre-tested and revised questionnaire. The survey was carried out during the rainy season (July to October 2022) by two trained investigators. The oral consent of the participants was taken after the presentation of the objectives of the survey. Translators were recruited locally and impregnated with the questionnaire to facilitate exchanges with the interviewees. The number of surveyed households varied from one department to another, depending on the edible insect’s consumers at the level of each surveyed village (Table 1). The collected data focused primarily on the socio-demographic characteristics of respondents (gender, age, ethnicity, religion, type of household, diet, income source, and education level), which are known as having a positive impact on respondents' willingness, perception, consumption, and acceptance of edible insects [21,22,23,24]. Among the respondents, 62.63% were men and the majority were illiterate (55.95%). The great majority of interviewees were married (80.17%) with a predominance of monogamous households (51.15%). Ottamari (37.79%), Yoruba (27.97%), and Dendi (18.8%) were the most represented ethnic groups. The respondents were Christians (45.93%), Muslims (29.44%), or Animists (24.63%). Agriculture was the main source of income (48.23%) of the surveyed households (Table 1). The second part of the survey was focused on the inventory of edible insects as well as the documentation of frequency of consumption, parts consumed, consumer preferences, seasonal availability, environmental factors affecting the diversity of edible insects, and traditional uses. The perception of the surveyed households on the edible insect’s consumption was assessed through a 5-point Likert scale (1 = completely disagree, 5 = completely agree) [9]. Samples of edible insects were also collected and stored in plastic boxes containing 70° ethanol for identification in the laboratory. Voucher samples were deposited at the Laboratory of applied entomology (LEnA) of the National High school of Applied Biosciences and Biotechnologies (ENSBBA).

Data analysis

The descriptive analysis was carried out to summarize the sociodemographic characteristics of the surveyed households. Chi-square (χ²) or Fisher test was performed to assess the relationship between insect consumption practices and ethnic groups. Principal component analysis (PCA) using the Facto Mine R package was performed [25] to describe the different uses of edible insects and respondents' perceptions of insect consumption with respect to ethnic groups.

The use value (UV) of each of the edible insect species inventoried in the study area was calculated following Alves et al. [26] using the formula:

$${\text{UV}} = \sum {\frac{{{\text{Ni}}}}{{{\text{Nt}}}}}$$

where Ni is the number of respondents who mentioned the use of the edible insect species, and Nt is the total number of respondents.

The fidelity level (FL), the percentage of respondents claiming the use of a certain species of edible insects for the same major purpose, was calculated for the most frequently reported diseases or ailments as:

$${\text{FL }}\left( \% \right) \, = \, \left( {{\text{Np }}/{\text{ N}}} \right) \, \times \, 100$$

where Np is the number of respondents that claim of use an edible insect’s species to treat a particular disease, and N is the number of respondents that use the edible insects as a medicine to treat any given disease [27].

Hierarchical Clustering on Principal Components (HCPC) was performed to assess the influence of social, cultural, and economic characteristics of respondents on the perception of insect consumption. The HCPC consisted of a Multiple Correspondence Analysis (MCA) realised on the perception measures and on the social characteristics of the respondents, followed by a hierarchical clustering analysis (HCA) on the principal components from the MCA. To select the variables for each perception group, we measured the difference between the class values and the overall values. These statistics were converted into a criterion called the value test (V-test) to perform a selection on the variables, and thus determine the most characteristic variables [28, 29]. The most characteristic variables of a group were those for which the test of associated values was greater in absolute value than 2. Moreover, if this test of value was positive for a variable, it had a high value in the class considered. On the other hand, if the value was negative, the variable had a low value for the class.

A multivariable logistic regression of the outcome of edible insect consumption (No/Yes) on the independent variables (age group, gender, ethnic group, religion, education level, main source of income, and market accessibility) was conducted to determine their association. The conditional association of the different independent variables with the outcome was quantified using odds ratios.

All the analyses were performed in R version 4.3 [30] and a significance level of 5% was considered. The graphs for the descriptive analysis were constructed using the package ggplot2.

Results

Consumption patterns of edible insects

The majority of the surveyed people (79.1%) were still consumers of edible insects and since several years ago (29.1 ± 17.2 years). The remaining surveyed people (20.9%) have consumed edible insects at least once in the past but no longer consume them. Most of the surveyed men (49.7%) consume edible insects against 29.4% of the surveyed women. Contrary to the other departments where the consumption of insects by the surveyed people is more recent, those surveyed in the Atacora department have consumed them since their childhood (39.3 ± 14.3 years of consumption on average). Among the nutrient’s sources of the surveyed households, edible insects represent 10 and 30% of annual intake (Table 2). About 33.6% of the surveyed people consumed edible insects at least once a month and only 21.3% consume them almost daily. There were no significant differences according to gender (p = 0.795) and level of education (p = 0.402) in the frequency of consumption of edible insects. Throughout the study area, insect consumption is usually family-based (79.3%). However, in the plateau department, entomophagy is much more practiced by parents (Table 2). Fried insects (71.8% of the surveyed people) are the preferred form of consumption in the study area. A difference in terms of the frequency of insect consumption was observed across departments (p < 0.001), age (p =  < 0.001), and religion (p =  < 0.001). More illiterate (44.9%), Christian (37.9%), and adult (57.4%) respondents continue to practice entomophagy compared to others (Fig. 2).

Table 2 Consumption frequency and use patterns of edible insects in the study area

Fig. 2

Variation in consumption of edible insects depending on A gender, B educational level, C religion and D age in the study area

Motivation for insect consumption

Eight reasons were mentioned by the surveyed people for insect consumption (Fig. 3). The delicious taste of edible insects (85.6%) and the cultural values (44.3%) associated with them were mentioned as the most important reasons. The good taste similar to fish (31.7%), the seasonal fish scarcity (23.2%), the food diversification, and the ease of preparing edible insects (28.2%) were listed by some households as reasons for insect consumption. While, some surveyed households consume edible insects specifically for their fatty content (15.5%), by pleasure (8.1%), or by lack of money (4.4%).

Fig. 3

Incentives for insect consumption in the study area

Diversity of edible insects

Insect species belonging to 17 genera were used as food and that some were identified to species level in the study area (Table 3). The inventoried insects belong to 7 scientific families with the order of the Orthoptera (52.9%) being the most represented. Fourteen, 7 and 6 edible insect species were listed respectively in the departments of Atacora, Plateau, and Zou. While, only 3 edible insect species were listed in the Alibori department. In the study area, the most consumed insects were Brachytrupes membranaceus Drury (Fig. 4), followed by Macrotermes subhyalinus Rambur, Macrotermes bellicosus Smeathman, and Rhynchophorus phoenicis Fabricus (Table 3). However, only, B. membranaceus was consumed by people in all the surveyed departments. The insect species consumed by the surveyed people varied according to ethnic groups (Fig. 5). Oryctes monoceros Olivier, R. phoenicis, and Macrotermes ivorensis Grassé & Noirot were more consumed by Yoruba, Fon et Adja ethnic groups. While Acanthacris ruficornis citrina Serville was more consumed by the Dendi ethnic group. The thirteen remaining species were consumed mainly by the other ethnic groups in the study area (Fig. 5).

Table 3 Insect species consumed by respondents in the study area

Fig. 4

Pictures of some edible insects collected in Southern Benin. Scale: 1:1. A Brachytrupes membranaceus; B Lampetis gorilla (Thomson); C Lava of Oryctes ohausi Minck; D Truxalis sp; E Adult of Rhynchophorus phoenicis Fabricius; F Lava of Rhynchophorus phoenicis, Fabricius; G Adult of Oryctes ohausi Minck; H Ornithacris turbida cavroisi

Fig. 5

Distribution of edible insects according to the surveyed sociolinguistic groups. Zon: Zonocerus variegatus, Tru: Truxalis spp, Cyb: Cybister sp., Lam: Lampetis gorilla, Rus: Ruspolia differens, Miv: Macrotermes ivorensis, Gry: Gryllus bimaculatus, Bra: Brachytrupes membranaceus, Msu: Macrotermes subhyalinus, Spa: Spathosternum pygmaeum, Mbe: Macrotermes bellicosus, Aca: Acanthacris ruficornis citrina, Orn: Ornithacris turbida cavroisi, Ory: Oryctes monoceros, Hie: Hieroglyphus africanus, Mfa: Macrotermes falciger, Rhy: Rhynchophorus phoenicis

Factors influencing the accessibility to edible insects

The collection of edible insects was done mainly in the wild with a period of accessibility coinciding mainly with the rainy season. Edible insects are mainly collected from crop fields (38.3%), houses (37.3%), savannas (15.2%), forests (3.4%), public places (2.9%), and mountains (2.9%). Six factors affecting the accessibility to edible insects were identified in the study area (Fig. 6). The most important factors were chemical pollution in edible insect collection fields (91.6% of surveyed people), climate variability (87.8%), and destruction of ecosystems (78.4%). Twenty-five chemical pesticides were perceived by the surveyed people as contributing to the chemical pollution of edible insect collection fields. Among them, 10 herbicides, 13 insecticides, and 2 fungicides were recorded (Table 4). The most used herbicides in edible insect collection fields are Nicomais 40 SC (12.1% of responses) and Kalach Extra 70 SG (9.8%) while Sunpyrifos 48% EC (12.7%) was the most cited insecticide.

Fig. 6

Factors affecting the accessibility to edible insects in the study area

Table 4 Pesticides used in crop fields where edible insects are collected by the surveyed consumer people

Edible insect uses

Besides their food use (63.2%), edible insects in the study area were used in traditional medicine (19%), spiritual uses (8.8%), poultry feed (6.1%), animal trap (1.3%), sold in markets (1%), and for fishing (0.6%). The use values of edible insects in the study area varied from 0.14 (Cybister sp.) to 1 (B. membranaceus) (Table 3). Eight edible insect species were used by the surveyed people of Atacora and Plateau departments to treat diverse ailments (Table 5). These insects are often mixed with honey for oral administration or shea butter for topical application. Among all the species of edible insects inventoried, fried grasshoppers (A. ruficornis citrina) and tobacco crickets (B. membranaceus) are the only ones listed as sold in markets mainly in the Alibori department (Fig. 7). These insects were sold either in piles or with a local measuring instrument called "Togolo" whose price varied depending on the insect and the period. Using the local "Togolo" measure, grasshoppers and crickets were sold at 1500FCFA and 2000 FCFA respectively in the dry season (November to March). While, in the rainy season (April to October), the price was falling ranging from 1000 to 1500FCFA for one "Togolo" of grasshoppers and crickets, respectively. In the Plateau department, R. phoenicis and Spathosternum pygmaeum Karsch were used by some people as bait to catch freshwater fish. While, B. membranaceus and Gryllus bimaculatus De Geer, M. bellicosus, M. falciger, Ornithacris turbida cavroisi, and S. pygmaeum were used for animal trap and poultry feed by some interviewed people of Atacora department. Except for A. ruficornis citrina and Cybister sp., the others listed as edible insects are used for spiritual purposes by the people interviewed mainly in the Atacora and Alibori departments.

Table 5 Edible insect species used in traditional folk medicine in the study area

Fig. 7

Some edible insect species sold in the Malanville markets

The results of the principal component analysis showed that the factorial design formed by axis 1 and 2 explains 88.6% of the initial total variability of the different traditional uses of edible insects by ethnic groups (Fig. 8). The analysis showed that most surveyed people of Ottamari ethnic group used edible insects for traditional medicine and for animal trapping. While the surveyed people from the Bariba ethnic group mainly use edible insects for spiritual purposes. On the other hand, the surveyed people from the Adja and Cotimba ethnic groups mainly use edible insects to feed poultry. Respondents from the Dendi ethnic group are mainly involved in selling edible insects. While those of the Yoruba ethnic group use them for fishing. The majority of surveyed people of the Fon and Peulh ethnic groups used edible insects only for food.

Fig. 8

Results of Principal Components Analysis to describe the relationship between ethnic groups and traditional use of edible insect

Perception of entomophagy

The factorial design formed by the first two axes explains 70.3% of the initial total variability of the different perceptions of entomophagy made by the respondents (Fig. 9). The analyses revealed that the Linkert scale such as disagree and strongly disagree are positively correlated with axis 1 in contrast to those of agree or strongly agree. Axis 2 is negatively correlated with the neutral scale, don't know. In general, the overall pattern of different assessments towards entomophagy perception showed that respondents are not afraid to eat insects. The majority of respondents consider edible insects to be nutritious, and feel good after eating them and therefore, shows a willingness to purchase edible insects as a new manufactured product. More than half of the respondents consumed insects because of their delicious taste (85.6%) and 31.7% consumed them for their fish-like taste. Some respondents (40.3%) consumed insects because of the cultural values associated with them. However, the surveyed people do not recommend to guests or teach their friends to eat insects. It also appears from their response that edible insects are no longer available as before and they do not trade insects and therefore do not make them a source of income. Furthermore, they are unaware of the effect of insect consumption on the health and well-being of children, and also of the impact of climate change on the availability of insects.

Fig. 9

Results of Principal Components Analysis to describe respondents' perception of insect consumption. ILE I like to eat edible insects; INA I’m not afraid to eat edible insects or when I see them; EIN Edible insects are nutritious; ILT I like the taste of edible insects; EIGH Edible insects are good for the health; WFG When I eat edible insects, I feel good; CET The capture of edible insects is easy for me at any time in the year; EIGCH Edible insects are good for children’s health; ITCFC I teach my children and my friends to capture edible insects; ITCCEI I teach my children to consume edible insects; ITFCEI I teach my friends to consume edible; IRGEEI I recommend to my guests to eat edible insects; IGEIEG I give edible insects to eat to my guests; IEEICI I eat edible insects because it's part of my cultural identity; EIGI Edible insects generate me income; TCBFCEI Those who can afford to buy fish also consume edible insects; EIMANP Edible insects are more available now than in past years; CVIAEI Climatic variations greatly influence the availability of edible insects; IBEIMBP I will buy the edible insects when they are marketed with beautiful packaging; ISEIMBP I will sell edible insects when they will be marketed with beautiful packaging; Disagree Disagree; DnKnow Do not know; StrAgree Strongly Agree; Agree Agree; StrDisagree Strongly Disagree

The results of the Hierarchical Clustering on Principal Components (HCPC) showed that 79.52% of the initial variability of the perception measures and social characteristics was retained on the first 60 axes of the Multiple Correspondence Analysis (MCA). The hierarchical clustering analysis (HCA) identified three groups of perceptions related to sociocultural and economic characteristics (Fig. 10).

Fig. 10

Projections of respondents in the scheme formed by the first two axes of the HCA. Cluster1: Insect consumers with a very negative perception of entomophagy; Cluster 2: Insect consumers with a positive perception of entomophagy; Cluster 3: Insect consumers with a very positive perception of entomophagy

The first cluster, which included 14.31% of respondents was characterized by consumers with a very negative perception of entomophagy and are not ready to promote it (Table 6). These respondents are notably Ottamari (55.22%; p < 0.001) and Cotimba (13.43%; p = 0.004), animists (38.81%; p = 0.001) with a high level of education (secondary: 35.82%, p < 0.001; university: 8.96%, p = 0.001) and whose frequency of insect consumption is average (38.81%, p < 0.001). The second cluster included 59.40% of the respondents mainly Yorouba (36.69%, p < 0.001) and Ottamari (50.72%, p < 0.001) consumers with a positive perception of entomophagy and its promotion (Table 7). The majority of these respondents are adults (74.82%, p = 0.003) and older persons (11.87%, p < 0.001) who practice the religion of Christianity (57.91%, p < 0.001) and who have a high frequency of insect consumption (73.74%, p < 0.001). However, these respondents are unaware of the health benefits of eating edible insects and do not adhere to their sales. The third group including 26.29% of the respondents belonging to the Dendi (65.85%, p < 0.001) and Fon (21.95%, p < 0.001) ethnic groups, practicing Islam (73.17%, p < 0.001) who are very favourable to entomophagy and its promotion (Table 8). This acceptability of entomophagy is associated with a young age (32.52%; p < 0.001) and a low frequency of insect consumption (32.52%; p < 0.001). They have knowledge of the health benefits of edible insects and are willing to buy them despite their low monthly consumption.

Table 6 First grouping of perception variables according to socio-cultural and economic variables

Table 7 Second grouping of perception variables according to socio-cultural and economic variables

Table 8 Third grouping of perception variables according to socio-cultural and economic variables

Factors determining insect consumption

The test of chi-square or Fisher exact showed strong evidence (p < 0.001) that the proportion of participants who eat the insect is associated with the ethnic group, and religion and also linked to their accessibility of market (Table 9). On the other hand, there was no evidence that the consumption of the insect is associated with gender (p = 0.92), age group (p = 0.21), education (p = 0.54), and source of income (p = 0.98). The results of the full model taking into account all the factors indicated that the covariates considered in this model, with the exception of the ethnic group, religion, and market accessibility, were no evidence (p > 0.05) on edible insect consumption. Thus, this model leads to the same results as the univariate tests performed on each of the factors. Therefore, edible insect consumption is associated with an ethnic group, religion, and market accessibility. Indeed, more Ottamari respondents (p = 0.004) consume insects than other ethnic group participants (Table 10). And the Muslim respondents (p = 0.03) consume weakly insects than Christians and animists. As access to the market becomes increasingly difficult (p < 0.001), people consume more insects (Table 10).

Table 9 Factors influencing entomophagy practice in the study area

Table 10 Result of multivariable logistic regression analysis

Discussion

Our results show that the consumption of insects goes back a long time and more frequent in the Atacora region than in other prospected regions. In fact, the population of this region is more affected by food insecurity [31], relies more heavily on entomophagy to meet their nutritional needs [12]. However, a small proportion (10–30%) of edible insects contribute to the nutrient’s intake of the surveyed populations with low-frequency consumption. This is not surprising, because some studies showed that insects are the least accepted alternative sources of nutrients among the population [32, 33]. Nevertheless, it would be interesting to assess the nutritional impact of edible insects in the function of their frequency consumption in Beninese households. In addition, knowing that the form of consumption can affect the digestibility and the nutritional properties of edible insects, therefore, it is important to check whether the fried form preferred by the Beninese populations allows optimal assimilation of nutrients.

A difference in the frequency of insect consumption was observed with regard to the level of education, age, and religion of the respondents. Indeed, it is known that certain religious prohibitions can slow down entomophagy [4], and that education can lead to the abandonment of the consumption of insects [34]. In addition, several studies have shown that young people are more inclined to entomophagy than older people mainly because of their openness [35, 36] Like Zimbabweans [37] and Ivorians [38], the delicious taste of insects is one of the main reasons for their consumption. In the study area, the cultural values associated with certain species of insects also contribute to the perpetuation of their consumption from generation to generation. Indeed, Hlongwane et al. [4] and Ghosh et al. [11] showed that culture plays an important role in entomophagy acceptance and edible insect preference.

Our results showed that 17 species of insects are consumed in the four surveyed regions. This number of edible insect species is low comparatively to other studies conducted in Benin [14, 15], and could reflect a gradual disappearance of some species or the decreasing trend of insect availability. Among the recorded species, Lampetis gorilla (Thomson) (Coleoptera: Buprestidae) to our knowledge has never been mentioned as edible insect in Benin. This could suggest that entomophagy is experiencing a positive development in Benin with new species of insects consumed. The dominance of Orthoptera, Blattaria, and Coleoptera among the edible insects in Benin is not surprising. Indeed, these insects’ orders are in the top five of most commonly consumed in Sub-Saharan Africa [39], and across the world [40, 41]. As mentioned by Ebenebe et al. [42], in Nigeria, the Tobacco cricket B. membranaceus is among the most preferred edible insects.

The use of chemical pesticides to protect farmlands was the main constraint hindering the accessibility of edible insects to populations. Indeed, edible insects are mainly caught in cropping areas, where they are also considered by some farmers as crop pests and killed with pesticides. In fact, some studies showed that edible insects collected in crop fields have a high quantity of pesticide residues on and recommended their farm rearing according to appropriate regulations to ensure safety [43, 44]. It would therefore be interesting to assess the level of pesticide residues contained in the main edible insects consumed in Benin. It is therefore urgent to develop simple methods of producing edible insects, accessible to low-income populations in order to guarantee the security of populations and contribute to poverty reduction.

Diverse uses of edible insects were recorded in the study area. As in other countries, edible insects are used for medicinal purposes [45,46,47], cultural rituals [48], and for poultry feed [49]. The market dedicated to edible insects is very little developed in Benin and remains informal. Indeed, Riggi et al. [14] mentioned the collection and informal sale of edible insects by the Nagot in southern Benin towards Nigeria. The influence of market accessibility on edible insect frequency consumption is not surprising. Indeed, respondents who have difficult access to the market consumed the traditional local products available to them to meet their nutritional needs and consider edible insects which are also of the same quality than the ‘meat’ from traditional sources [50] as their main food source. The grasshopper species (A. ruficornis citrina and B. membranaceus) mentioned as sold in the markets of Alibori department were also cited as having a commercial value in Nigeria [42], and Cameroon [51]. It is therefore appropriate to create a value chain around edible insects and thus contribute to the establishment of a formal market.

The perception of entomophagy and the frequency of consumption of edible insects depends on ethnic groups. In fact, Dendi, Fon, Yorouba and Ottamari ethnic groups showed a good perception of entomophagy and clear desire to promote it. However, some respondents of Yorouba and Ottamari ethnic groups are unaware of the health benefits of eating edible insects. This ignorance of the nutritional benefits of edible insects despite their consumption could be explained by the fact that they consume the insects only by food habit or just for flavour. It is, therefore, necessary to organize awareness-raising on the nutritional benefits linked to the consumption of edible insects towards this type of consumer to reinforce their inclusion as a regular source of nutrients in their diet.

Our results showed that respondents from the Cotimba and Ottamari ethnic groups, who still practice endogenous religions have a negative perception of entomophagy compared to those who are Christians or Muslims. Indeed, it is known that religions prohibiting the consumption of animals and their derivatives significantly influence the practice of entomophagy [52, 53]. It is therefore not surprising that religion was found to be an important factor influencing consumption frequency in the study area. In addition, eating insects is not prohibited for Christians and Muslims because it is mentioned in various literature related to these religions [54, 55]. However, knowing that the cultural realities [56] and some religions such as the Zionist churches in South Africa [57] or the apostolic churches in Zimbabwe [37] can negatively influence insect consumption, it is important to evaluate the effect of religious and cultural beliefs on entomophagy practice in Benin in order to define appropriate policies for its promotion.

Conclusion

Our results showed that the consumption of edible insects represents a small part of the total food intake, and is however fully rooted in the eating habits of the surveyed people. Several reasons motivating the consumption of insects have been recorded and must be exploited in the definition of policy to promote entomophagy in the study area. Various synthetic chemical pesticides are used in edible insect collection fields suggesting the possibility of consuming insects containing residues harmful to the health of populations. The development and popularization of edible insect farming methods accessible to low-income populations is therefore recommended. In addition, awareness sessions on the nutritional benefits associated with the consumption of edible insects must be organized for their regular inclusion in the diet of populations. A differential perception of entomophagy across ethnic groups was noted with a negative perception among the Cotimba ethnic group. Ethnicity and religion are the main factors taken into account in the definition of policies to promote entomophagy in Benin.