Importance of sigma factor mutations in increased triclosan resistance in Salmonella Typhimurium

Salmonella enterica is the second most common foodborne pathogen. The use of biocides is crucial to prevent spread of foodborne pathogens, and it would be devastating for food safety if Salmonella would become resistant to the disinfectants used. Another concern is that exposure to disinfectants might lead to decreased susceptibility to antibiotics. The current study aimed to identify genetic changes causing high level triclosan resistance in S. enterica serovar Typhimurium and evaluate how these affected antibiotic resistance and efflux pump activity. Wild type strains S. Typhimurium 4/74 and DTU3 were adapted to increasing concentrations of the biocide triclosan by serial passage. High level triclosan resistant isolates (MIC > 1000 μg/ml) were obtained. Strains were genome sequenced, and SNPs in fabI, rpoS and rpoD were found to be associated with high level resistance. However, work with defined mutants revealed that a SNP in fabI was not sufficient to obtain high level resistance. This required additional mutations in the sigma factors rpoS or rpoD. The adapted strains showed triclosan-dependent increased efflux, increased fabI expression and reduced susceptibility towards the antibiotics enrofloxacin and sulphamethoxazole/trimethoprim. Medium level triclosan resistance could be obtained by fabI mutations in S. Typhimurium, however, high level resistance was found to require sigma factor mutations in addition to a fabI mutation. Reduced antibiotic sensitivity was observed for the adapted strains, which could be associated with increased efflux.


Background
Salmonella continues to be an important foodborne pathogen with 91.000 confirmed human cases reported in Europe in 2012 [1]. Among the serovars involved, Salmonella enterica serovar Typhimurium (S. Typhimurium) was the second most important serovar representing 22% of all confirmed cases [1]. Levels of resistance towards clinically important antibiotics, such as ciprofloxacin, are high in isolates of this serovar from animals and food [2].
Biocides are broadly used to prevent microbial growth and play an important role in preventing the spread of pathogenic bacteria. In recent years there has been increasing concern that use of biocides can select for antibiotic cross resistance [3], in addition to causing increased tolerance towards the biocides themselves. During the last 30 years several cases of bacteria developing resistance or tolerance towards biocides, and in some cases cross-resistance to antibiotics, have been reported [4]. Recently, it has been shown that "in-use" concentrations of disinfectants can select multidrug resistant mutants of S. Typhimurium [5].
Biocides are used in a wide variety of applications, spanning from preservatives in household-products like vacuum cleaners to disinfectants, make-up and industrial cleaning agents [6]. Especially triclosan, a chlorophenol, has been used in many products, including toothpaste, soaps and as antibacterial agent in toys, fabrics and cutting boards. The effect of triclosan has been extensively studied. It has been shown that triclosan inhibits the enoyl-acyl carrier protein reductase (FabI) which is involved in fatty-acid biosynthesis in Escherichia coli [7]. In addition, low concentrations of triclosan interfere with nutrient-uptake, whereas high concentrations facilitates membrane leakage by incorporation into the bacterial membrane [8].
Efflux pumps play a role in exporting toxic compounds from the cell and could be a common mechanism for antibiotic and biocide resistance [9]. The efflux systems EmrAB/AcrEF have been found to play a role in the susceptibility of Salmonella towards triclosan [10]. Furthermore, inactivation of the efflux pump genes acrB and tolC in Salmonella has previously been shown to decrease triclosan resistance [11]. However, proteomic studies of various triclosan-resistant strains revealed that there was no significant overexpression of the AcrAB-TolC efflux-pump [12], indicating that efflux is not the main mechanism of triclosan resistance [13].
Different genes have been associated with reduced susceptibility to triclosan. Results from E. coli and S. aureus have implied that point mutations in fabI are the primary reason for decreased triclosan sensitivity and that fabI overexpression in E. coli is associated with triclosan resistance [14,15]. In accordance with this, up-regulation of fabI in Salmonella has been described in response to triclosan exposure, but point mutations or overexpression of fabI is not sufficient to give high-level resistance in this bacterium, indicating that there are other, yet to be determined, factors involved [11,16]. The aim of this study was to determine which mutations, in addition to mutation in fabI, are required for S. Typhimurium to obtain high level biocide resistance. To study this, we adapted two different isolates of S. Typhimurium to high level triclosan resistance and identified the genes involved in this resistance. In addition, we investigated the effect of the mutations on efflux activity, antibiotic cross resistance and cell culture invasiveness and growth.

Bacterial strains
Bacteria used in this study are listed in Table 1. To evaluate the effect of single SNPs (compared to wild type) from strains adapted to high level triclosan, a single SNP was isolated from other SNPs by transfer to a clean parent strain background by phage transduction using P22 phages. P22 transductions were performed with P22HT105/int − 201 as described [17]. A selective pressure of 2 μg/ml of triclosan was used to select for the transfer of the fabI SNP. An rpoS deletion-mutant was constructed by Lambda-Red mediated allelic exchange of the rpoS gene with the chloramphenicol cassette as previously described [18] using the primers rpoSfwd: 5′CAGAATACGCTGAAAGTTCATGATTTA AATGAAGACGCgtgtaggctggagctgcttc3′ and rpoSrev: 5′G CG GAA CAGCGCTTCGATATTCAGCCCCTGCG TCTGCAcatatgaatatcctccttag3′ in 4/74. The mutant was verified using a PCR-strategy with the primers rpoScon: 5′-GGATCACGGGTAGGAGCCACCTTTTG AG and camfwdny: 5′-TACGCAAGGCGACAAGGTG CTGATGCCG as previously described [19]. The deletion of rpoS was transferred to TDTU3C by P22 phage mediated general transduction. Mutations were confirmed by PCR and sequencing. Strains were maintained in LB-Lennox broth (LB) (BD Difco™). For growth on solid media, LB was added 1.5% agar producing LB agar plates.

Biocide solutions
A stock solution of 50 g/L triclosan (Irgasan, Sigma-Aldrich, Broendby Denmark) was made in 70% ethanol and from here a solution of 300 μg/ml was prepared in sterile MilliQ-water and kept at 5°C until precipitations had dissolved, usually 18-20 h.

Adaptation to high concentrations of biocides
Adaptation to high concentrations of biocides was obtained by daily sub-cultivations using an oblique-plate assay modified from assays previously described [20,21]. Briefly, 3x700μl MHB (Müller Hinton broth, Oxoid A/S, Roskilde, Denmark) was inoculated with one colony and incubated at 37°C with shaking for 3.5 h. Plates containing a maximum concentration of ½ MIC were streak- inoculated starting from the highest concentration in triplicate. After incubation overnight at 37°C colony material swabbed from growth at the highest concentration were suspended in 1 ml sterile 0.9% NaCl and sub-cultivated daily on the same concentration until growth occurred in the full length of the plate on two subsequent days, then the concentration was doubled. Adaptation was ended after five subsequent days using same concentration with no increase in the extent of growth. Control-adaptations were done on plates not containing biocide to examine the effect of subcultivations alone. Adaptation stability was examined by growth in media without biocide for five subsequent days with daily passage.

MIC-determinations
MIC determinations for biocides were done by micro dilution assay as previously described [22]. Strains adapted to biocides were tested in concentrations ranging from 8-4000 μg/ml and non-adapted strains (wild type and control-adapted strains) were tested in concentrations 0.14-64 μg/ml. All MIC determinations were done in technical duplicate and biological triplicate.

Antibiotic susceptibility tests
Susceptibility towards antibiotics was examined in biological triplicate using disk diffusion according to EUCAST guidelines version 3.0, 24 April, 2013 using Müller Hinton agar (MHA) (Oxoid A/S, Roskilde, Denmark). Furthermore antibiotic resistance tests were performed on MHA containing triclosan in concentrations of 1 and 1000 μg/ml. Statistical analysis was performed on zone diameters varying >2 mm compared to wild type.

MBC determinations
Minimum bactericidal concentrations (MBCs) towards biocide were estimated by spot plating 2x10μl on LB agar from inoculum and all wells without growth from the MIC-plate after incubation. MBC was determined as the lowest concentration giving more than a 5-log reduction of inoculum.

Genome sequencing and SNP detection
Both parent strains and all adapted strains including controls were sequenced. DNA was extracted from overnight cultures using DNeasy Blood and Tissue Kit (Qiagen) according to manufactures instructions. Genome sequencing was done at the National High-Throughput Sequencing Centre, University of Copenhagen, Denmark using Illumina HiSeq 2000 paired end reads with a coverage of 100-200 bp. Sequences were analyzed for the presence of SNPs and other genomic variations using CLC Genomic Workbench 5.5.1 probalistic variant detection after mapping to the annotated genome of S. Typhimurium strain 4/74 (CP002487) [23]. Variants of DTU3 were filtered against control-reads of DTU3wt with a control read count threshold of 20 prior to analysis. Mapping of 4/74 revealed 100% coverage and the mapping of DTU3 showed 98% coverage meaning that the sequenced obtained from DTU3 covered 98% of the published genome of 4/74, which primarily contained genes encoding phage proteins, prophages and other phage related structures.

Growth curves
Growth was evaluated with and without triclosan in a concentration of 0.3 μg/ml. Growth curves were obtained using Bioscreen C (Finland) in a final volume of 200 μl Müller Hinton broth (MHB). The inoculum was 5 μl overnight (ON) culture diluted to an OD 600 = 0.05, giving a final OD in the wells of 0,001 (about 1*10 6 CFU/ml).
Plates were incubated at 37°C in Bioscreen C with medium shaking for 10 sec before each measurement. OD 600 was measured every 20 min for 23 h. Growth curves were performed in biological triplicate.

Efflux pump activity
Efflux pump activity was evaluated using the Cartwheel method modified from [24]. The inoculum was prepared by growing the strains for 4 h in LB at 37°C with shaking. Efflux was evaluated in increasing concentrations of triclosan (0.25-1000 μg/ml) with fixed concentration of ethidium bromide of 1 μg/ml. Fluorescence was examined using GelDoc (Bio-Rad Laboratories, Inc.).

Cell culture infections
Cell-assays were performed using INT-407 (HeLa contaminated epithelial cell-line) as previously described [26] with few modifications: Cells were seeded at 5x10 5

Statistics
For the analysis of differences in antibiotic resistance a mixed linear model was used. Each concentration of triclosan was analyzed separately, and zone diameters of the adapted strains at triclosan concentration of 1000 μg/ ml were compared to wild type strains at triclosan concentration of 1 μg/ml. Zone diameter for each antibiotic were the response variable and strain were the explanatory variable. Date of antibiotic testing was included as a random factor. The statistical analysis was performed using the program R [27] and the packages "lme4" [28] and "multcomp" [29]. Student's t-test in Excel 2010 (Microsoft) was used to analyse differences in adhesion and invasion between strains. Significance levels were set at 5%.

Results
Salmonella Typhimurium isolates with tolerance to high concentration of triclosan are easily obtained after exposure to triclosan Two different S. Typhimurium strains, the clinical isolate 4/74 [30] and the pork slaughterhouse isolate DTU3 were exposed to triclosan to evaluate the ability to obtain resistant mutants and to elucidate the genetics behind this adaptation. Adaptation was done using daily sub-culturing on gradient plates containing increasing concentrations of triclosan. Three biologically independent adapted strains were isolated for each wild type strain. Before adaptation, the triclosan MIC values for 4/74 and DTU3 were 1 μg/ml and 8 μg/ml, respectively. After 36 sub-cultivations the MIC had increased to 1057-2088 μg/ ml for strain 4/74, subcultures A-C (from here on designated T4/74 strain A-C) and 2088-4124 μg/ml for TDTU3 strains A-C (Table 2). MBC-values, defined as the lowest concentration giving a 5-log reduction of inoculum, were shown to be 4124 μg/ml and 4124-8146 μg/ml respectively (Table 2). To evaluate the stability of the phenotype, the adapted strains were subsequently grown five days without triclosan and were found to maintain their high level resistance (data not shown). This indicated that the adaptation was caused by genetically inheritable mutations rather than development of phenotypic tolerance. The control strains K4/74 and KDTU3 were similarly exposed to 36 sub-cultivations, but without triclosan. Both control strains maintained the wild type MIC (Table 2).

Concurrent mutations in fabI and sigma-factor leads to high-level resistance
To elucidate the changes responsible for triclosan resistance, genome sequencing of the strains was conducted. The genome sequences of the adapted strains were compared to the corresponding control strains and revealed between two to seven changes (Table 3). Only non-synonymous changes in coding regions were considered. A Gly-93 → Ser mutation in fabI was present in all adapted strains with the exception of TDTU3C for which a Gly-93 → Val mutation in fabI was present. Mutations were also found in several other genes including rpoD and ndh (T4/74C) and rpoS (TDTU3C) ( Table 3). We focused our investigations on the two adapted strains, T4/74C and TDTU3C to elucidate the importance of the SNPs in the sigma factors, rpoD and rpoS. To investigate whether mutations in the adapted strains had an effect on fitness, their growth was compared to the control strains. We found that the adapted strains T4/74C and TDTU3C and their corresponding control strains showed similar growth (data not shown). To evaluate the importance of the sigma factor SNPs, we constructed a mutant harboring only the fabI mutation by transferring the fabI SNP of T4/74C to the wild type strain 4/74, obtaining the strain 4/74-fabI. This strain had higher MIC (270 μg/ml) than parent wild type strain, but did not show the same high MIC to triclosan as the adapted strain, that also contained the ndh and rpoD mutations (Table 2). Similarly, the MIC of a DTU3-fabI mutant (64 μg/ml), without the SNP in rpoS was also found to be 4 times higher than the wild type, but 64 fold lower than the TDTU3C strain (Table 2). Introducing the rpoS mutation (R100H) into DTU3-fabI by phage transduction reestablished the high triclosan MIC, confirming that both mutations have to present to obtain high level resistance ( Table 2). The importance of a mutation in a sigma factor was consistent with the observations in the adapted strain TDTU3C, which only had mutations in the sigma factor rpoS in addition to the mutation in fabI. Furthermore, the significance of a functional sigma factor for high triclosan resistance was shown by deleting the rpoS gene in the TDTU3C strain resulting in the mutant TDTU3CΔrpoS::cam which had lost its high level resistance ( Table 2). Mutations in fabI was found in all our adapted strains and increased expression of fabI has previously been shown to affect triclosan resistance [11]. To evaluate if the SNPs identified altered the expression of fabI, the amount of fabI mRNA was compared between the adapted strains and the wild type. Examination of fabI expression using Northern Blot showed a minor up-regulation of fabI in the two adapted strains (data not shown). This corresponds with previously published observations of triclosaninduced fabI expression [11,31].

Antibiotic resistance and efflux pump activity in triclosan adapted strains
There is an increasing concern that adaptation to biocides could lead to cross resistance to other antimicrobial compounds, including antibiotics used in the clinical setting [6]. Antibiotic resistance analysis was done using disc-diffusion on plates with and without triclosan. When comparing wild type, control and adapted strains, we observed only slight decreases in zone diameter for the adapted strain T4/74C with enrofloxacin (ENR), and for TDTU3C exposed to sulfamethoxazole/trimethoprim (SXT),, indicating reduced sensitivity (Table 4). On the other hand, the adapted strain T4/74C showed a small increase in zone diameter regarding amoxicillin/clavulanic acid (AMC) and TDTU3C regarding ENR. However, when the tests were done using plates with triclosan in concentrations of 1000 μg/ml a highly significant decrease in zone-diameter was seen towards SXT (p < 0.001) and ENR (p < 0.001) for both T4/74C and TDTU3C ( Table 4). The opposite was observed regarding cefotaxime (CTX), where both of the adapted strains showed a significant increase in zone diameter, and similarly for 4/74C exposed to ceftiofur (EFT) (p < 0.001).
Triclosan resistance can be caused by efflux pumps which are also known to affect resistance to antibiotics [32]. Since we observed antibiotic cross resistance when high triclosan concentrations were present, we investigated whether increasing triclosan concentrations could lead to increased efflux. We evaluated efflux activity by using accumulation of ethidium bromide (EtBr) as an indicator of the level of efflux. EtBr traverses the bacterial cell wall and once inside it binds to DNA and fluoresces in ultraviolet light. Bacterial efflux pumps recognize EtBr and are able to extrude it to the medium meaning that low fluorescence is indicative of high efflux. Our experiments revealed a correlation between efflux and concentration, showing increased efflux when the adapted strain was exposed to triclosan in high concentrations (Fig. 1). At low concentrations (0,5 and 5 μg/ml), high level fluorescence was observed as a highly fluorescent streak of bacteria. This corresponds with high level of intracellular EtBr caused by low efflux. At 500 and 1000 μg/ml triclosan, the bacterial streak only shows low level fluorescence, which indicates high efflux and limited binding of EtBr to the DNA in the cell.

Cell culture adhesion and invasion of triclosan adapted strains
Previous results have shown a link between triclosan resistance and virulence factor expression in Gram positive bacteria and reduced invasiveness after prolonged treatment of S. Typhimurium with disinfectants [4,20]. We therefore investigated the ability of the adapted strains to adhere to and invade the cell-line INT407. We found a small, but significantly decreased ability of the adapted strain TDTU3C to adhere and invade compared to the wild type and control strains. However, the inoculum was also slightly decreased for the TDTU3C strain. 4/74C did not show reduced adherence and invasion compared to the control strain K4/74 (Fig. 2). The control strain K4/74 revealed a decreased ability to invade compared to the wild type, however it also showed a decreased adhesion, which could account for the lower ability to invade. Our results therefore indicate that the mutations do not have any major effect on virulence for the two strains.

Discussion
In the present study, we have investigated the genetic background for the ability of S. Typhimurium to adapt to triclosan. Adaptation was found to be relatively easy and we were able to adapt two different strains of clinical and slaughterhouse origin to grow in concentrations equal to or above 1000 μg/ml. The mechanisms of resistance towards triclosan have been studied quite intensively, but still the exact mechanisms are not fully understood [11-13, 16, 33-36]. To further elucidate the genetics behind this adaptation, we genome sequenced the adapted strains and compared the sequence to the control strains. In all adapted strains, a mutation was present in fabI, however other mutations were also present. From our genome sequencing results and subsequent construction of mutants containing fabI from the adapted strains, we can conclude that having a mutation in fabI leads to increased triclosan tolerance, but is not enough to confer high level resistance.
Our results show that mutations in one of the sigma factors rpoS or rpoD, in addition to the fabI mutation, caused high resistance levels in T47/4C and TDTU3C. This corresponds with previous results showing that fabI mutations are involved in triclosan resistance, but do not alone account for high level resistance [11]. The authors demonstrated that a variety of other genes, including arcB, tolC and ramA can be involved in triclosan resistance [11]. Although, it has been shown, that triclosan induce rpoS expression in S. Typhimurium [16], implying its importance for a triclosan response, In addition to the fabI and sigma factor mutation, we also found a mutation in ndh in T4/74C. The function of ndh is not fully understood, but it has been proposed that it is involved in rapid NADH recycling [37]. Since triclosan is known to bind NADH, it is not unlikely that this mutation has some additional effect on susceptibility or fitness of the strain. However, since we have found no mutations in ndh or other similar genes in TDTU3C, we did not consider the ndh mutation to be as important as the mutation in the sigma factor. It is, however, important to notice, that high level resistance to triclosan can be achieved with no mutations in sigma factors or ndh, as evident from the adapted strains T4/74A, TDTU3A, and TDTU3B. Further studies are needed to elucidate whether such strains harbours mutations in genes that are directly or indirectly regulated by RpoS or RpoD, eliminating the need for mutation in the regulatory gene(s).
Several researchers have been able to adapt Salmonella and other bacteria to certain biocides and the concern is that the mechanisms resulting in biocide resistance may confer cross resistance to antibiotics [4,38,39]. In this study, we found a decreased susceptibility of T4/74C and TDTU3C towards enrofloxacin and sulfamethoxazole/trimethoprim, respectively.
A highly interesting observation was that concurrent exposure to high concentrations of triclosan conferred a marked decrease in zone diameters of the adapted strains a b c d towards enrofloxacin and sulfamethoxazole/trimethoprim. A possible explanation could be the induction of efflux by triclosan. We found that increased efflux of EtBr was linked to increased concentration of triclosan ( Fig. 1) supporting the hypothesis that high triclosan concentrations induce efflux and thereby explain why cross resistance to antibiotics is primarily observed when the bacteria are also subjected to high concentrations of triclosan. This is likely since resistance towards SXT and fluoroquinolones can occur via efflux. The ArcAB-TolC efflux pumps are important for triclosan resistance [11] and these pumps have also been shown to directly contribute to flouroquinolone (e.g. ENR) resistance in Salmonella [40,41]. The efflux systems EmrAB and ArcEF have been shown to be important for both triclosan resistance [10] and trimethoprim resistance [42]. Our results support the previous observations of triclosan dependent increase of antibiotic resistance in different bacteria [43][44][45][46]. Our data demonstrated that the mutations in T4/74C only had minor effects on adhesion and invasion to a cultured cell line. In contrast to this, the mutations in TDTU3C did have significant effect on adhesion and invasion, however, the inoculum was slightly lower for this strain compared to the wild type strain, and this might explain this difference. Previous work on triclosan exposed S. Typhimurium has showed reduced ability to invade Caco-2 epithelial cells [4]. This difference could be due to differences in cell lines and different mutations in the strains investigated.

Conclusion
The current study has demonstrated that high level resistance to triclosan can be achieved by mutations in both fabI and in a sigma factor. The combinations of these mutations do not compromise growth, but induces efflux, which in turn may be accountable for the observed antibiotic resistance. Further research is needed to clarify the role of sigma factor mutations in high level triclosan resistance.