De novo sequencing of the Hypericum perforatum L. flower transcriptome to identify potential genes that are related to plant reproduction sensu lato

St. John’s wort (Hypericum perforatum L.) is a medicinal plant that produces important metabolites with antidepressant and anticancer activities. Recently gained biological information has shown that this species is also an attractive model system for the study of a naturally occurring form of asexual reproduction called apomixis, which allows cloning plants through seeds. In aposporic gametogenesis, one or multiple somatic cells belonging to the ovule nucellus change their fate by dividing mitotically and developing functionally unreduced embryo sacs by mimicking sexual gametogenesis. Although the introduction of apomixis into agronomically important crops could have revolutionary implications for plant breeding, the genetic control of this mechanism of seed formation is still not well understood for most of the model species investigated so far. We used Roche 454 technology to sequence the entire H. perforatum flower transcriptome of whole flower buds and single flower verticils collected from obligately sexual and unrelated highly or facultatively apomictic genotypes, which enabled us to identify RNAs that are likely exclusive to flower organs (i.e., sepals, petals, stamens and carpels) or reproductive strategies (i.e., sexual vs. apomictic). Here we sequenced and annotated the flower transcriptome of H. perforatum with particular reference to reproductive organs and processes. In particular, in our study we characterized approximately 37,000 transcripts found expressed in male and/or female reproductive organs, including tissues or cells of sexual and apomictic flower buds. Ontological annotation was applied to identify major biological processes and molecular functions involved in flower development and plant reproduction. Starting from this dataset, we were able to recover and annotate a large number of transcripts related to meiosis, gametophyte/gamete formation, and embryogenesis, as well as genes that are exclusively or preferentially expressed in sexual or apomictic libraries. Real-Time RT-qPCR assays on pistils and anthers collected at different developmental stages from accessions showing alternative modes of reproduction were used to identify potential genes that are related to plant reproduction sensu lato in H. perforatum. Our approach of sequencing flowers from two fully obligate sexual genotypes and two unrelated highly apomictic genotypes, in addition to different flower parts dissected from a facultatively apomictic accession, enabled us to analyze the complexity of the flower transcriptome according to its main reproductive organs as well as for alternative reproductive behaviors. Both annotation and expression data provided original results supporting the hypothesis that apomixis in H. perforatum relies upon spatial or temporal mis-expression of genes acting during female sexual reproduction. The present analyses aim to pave the way toward a better understanding of the molecular basis of flower development and plant reproduction, by identifying genes or RNAs that may differentiate or regulate the sexual and apomictic reproductive pathways in H. perforatum.


Background
Reproduction is the highest priority of all living things, and involves a complex combination of processes whose variability between species has long puzzled evolutionary biologists. In angiosperms, reproduction for the most part culminates in the formation of male and female gametophytes, whose union leads to a new sporophyte generation that will begin from seeds. In the flower, male and female sporogenesis and gametogenesis generate two types of gametes that are either enclosed in an embryo sac (egg cell), or pollen grain (sperm).
Despite the enormous complexity and heterogeneity of flower morphology existing within angiosperms, a number of recent publications focusing on the genes involved in flower development suggested that the biological basis of flower development is actually highly conserved among plants [1]. Accordingly, the genetic and epigenetic bases of gamete formation have been studied in model species, such as Arabidopsis, snapdragon and maize, using a number of different approaches. The production and characterization of mutants defective for their ability to set seeds [2,3], in addition to expression studies [4], led to the identification of several genes that are potentially or effectively associated with sporogenesis and gametogenesis [5]. A number of mutations affecting the normal progression of meiosis [6] and gametophyte development [2,7], as well as double fertilization and seed development [8], have been studied in great detail (reviewed by [5]). Interconnections between the ovule and the developing embryo sac have emerged in a number of studies in which sporophytic mutations directly affecting gametophytic development were studied [9][10][11]. More recently, the mutation of genes involved in the production and action of sRNAs in the ovule have been shown to have dramatic effects on the development of the embryo sac [12,13], thus supporting the idea that sRNA-related mechanisms residing in the sporophyte are critical for cell-fate determination in the ovule and the formation of gametes [13][14][15].
Increasing interest has been focused on Hypericum perforatum for the study of apomixis, a naturally occurring form of asexual reproduction whereby progeny inherit the entire maternal genome through the seed [16][17][18][19][20]. Hypericum spp. (1.3 pg/2C, equal to ∼630 Mb) have x = 8 chromosomes: individuals are mainly tetraploid with 2n = 32, but diploid and hexaploid individuals have been found. H. perforatum is an invasive perennial herb that is widely distributed in a variety of habitats and is regarded as a serious weed in many countries [21,22]. Several compounds produced by Hypericum species have stimulated the interest of the scientific community for their biological activity [23], and H. perforatum has been studied for the identification of potential genes involved in the biosynthesis of active metabolites [24]. From the reproductive point of view, H. perforatum reproduces via aposporic apomixis, a gametophytic variant (according to Nogler, [25]) whereby the alternative differentiation of a somatic cell gives rise to a functional, unreduced embryo sac. In principle, aposporic initial cells in apomictic plants are somatic cells belonging to the nucellus, which change their fate by being able to mitotically divide and develop functional embryo sacs through mimicking sexual gametogenesis development [19,26]. The mode of reproduction in H. perforatum is highly dynamic, and biotypes span from almost complete sexuality to nearly obligate apomixis. In particular, apomixis is mostly found in tetraploid individuals and is characterized by complete penetrance and variable levels of expressivity, ranging from 20% to 97% (for review, see Pupilli and Barcaccia, [27] and ref. therein). The occurrence of diploid and hexaploid individuals reflects a dynamic reproductive system because haploidization and polyploidization are mediated by parthenogenesis of meiotic egg cells and fertilization of aposporic egg cells, respectively [17,26]. As with other asexual plant complexes, apomixis and hybridization are closely linked in H. perforatum [28], and interestingly the dosage of genetic factors has been proposed to influence the penetrance of apomixis, as tetraploid and hexaploid genotypes tend to be more apomictic and sexual respectively, regardless of geographic origin [29]. These observations are in agreement with the hypothesis that apomixis might relies upon spatial or temporal mis-expression of genes acting during female sexual reproduction [30].
Hypericum perforatum is considered an attractive model system for the study of apomixis because it is characterized by a relatively small genome size, the availability of morphologically distinct ecotypes, self-compatibility and easy cross-ability, high degree of molecular polymorphisms, along with a versatile mode of reproduction, a relatively short generation time and an abundant seed set [26,31]. Genotypes that produce embryos either from aposporic fertilized egg cells or from parthenogenesis of meiotically reduced egg cells have been identified, suggesting that apospory and parthenogenesis may be developmentally uncoupled [17]. It is now well known that parthenogenic capacity is preferentially expressed by aposporic egg cells (or, alternatively, non-parthenogenic behavior is frequently associated with meiotic egg cells). Nevertheless, aposporic egg cells can frequently occur in non-parthenogenic individuals, and parthenogenic development of meiotic egg cells can also take place. Genotypes that almost exclusively express only one component of apomixis while suppressing the other support the hypothesis that two distinct genetic factors control apospory and parthenogenesis in this species (for review, see Barcaccia et al., [27] and ref. therein). Recently, the genetic basis of apomixis in H. perforatum was uncovered by mapping and then sequencing a locus (designated HAPPY for Hypericum APOSPORY) associated with apospory, demonstrating that distinct genetic factors are associated apospory and parthenogenesis in this species [18].
Recently, next generation sequencing (NGS) technologies have been used to investigate gene expression changes associated with sperm development [32], differentiation of the megaspore mother cell [33] and specific cell types of the embryo sac [34] in Arabidopsis. In an alternative approach, high-quality single nucleotide polymorphisms (SNPs) were mined from NGS libraries sequenced from a number of sexual and apomictic Ranunculus genotypes in order to elucidate the origin and evolution of apomixis [35]. Taken together, the availability of reference DNA and RNA datasets are increasing the ease at which complex phenotypes and processes in non-model organisms can be analyzed. Here we sequence and annotate the flower transcriptome of H. perforatum with particular reference to processes related to reproduction. The annotation and comparative investigation of the flower transcriptome is a critical step for better understanding the genetic control of apomixis, and provides valuable information on gametophyte determination and gamete formation in Hypericum.

Results
De novo assembly and annotation of the Hypericum flower transcriptome We analyzed two sexual and three apomictic accessions (two obligate and one unrelated facultative apomictic accession characterized by 24% apomictic seed development; Table 1; Figure 1, panel A). Flower buds and flower organs were collected at different developmental stages corresponding to Arabidopsis flower stages 1-12 and spanning meiosis and gametogenesis processes ( Figure 1, panels B-G, with particular reference to the female gametophyte).
Samples were collected to include the entire developmental pathway of gamete production, whose critical steps in sexual ovules include megaspore mother cell differentiation ( Figure 1, panel B), its meiotic division to form a functional megaspore (Figure 1, panel C) and mitotic divisions giving rise to a functional eight-nucleate embryo sac with an egg cell apparatus (Figure 1, F-G). Conversely, aposporic ovules ( Figure 1, panel I) are frequently characterized by a failure of the meiotic program and differentiation of one or multiple unreduced embryo sacs from the somatic cells (aposporic initials) of the ovule (Figure 1, panels D-E).
All full-length enriched cDNA libraries were successfully normalized (data not shown) in order to avoid over representation of the most commonly transcribed genes and to maximize sequence diversity. Roche 454 sequencing of two full plates yielded a total of 1.47 million sequences, and an average number of sequenced reads from each library of 177,822 (Additional file 1: Table S1).
High quality sequences from all libraries were pooled and assembled to create a single global reference transcriptome, and yielded 33,860 isotigs with an average length of 1,002 bp and an N50 of 1,184 bp (Table 2). Following the assembly procedure, the quote of singletons was equal to 2% ( Table 2). As a result, the assembly pipeline produced 60,428 assembled sequences -from now onward defined as unigenes -that were composed of 33,860 isotigs (accounting for 98% of sequenced reads) and 26,568 singletons (Table 2).
BLASTX analysis aligned approximately 61% of the Hypericum unigenes to a protein present in the nonredundant (nr) database (n = 36,988; E-value ≤1.E-06; Table 3). The remaining 39% of assembled unigenes (n = 23,455; Table 3) did not yield significant matches at this level of stringency. The relative contribution of assembled and non-assembled reads to the annotation of the Hypericum flower unigenes was also computed with a BLASTX-based procedure (see Additional file 1: Table S2). As shown in Table 3, 90% of the best hits produced by BLASTX were concentrated in 10 species, whereas the remaining 10% of BLASTX hits were distributed over 632 taxonomic entities. Roughly 86% of the most significant alignments were made with proteins deduced from Ricinus communis (35%), Populus trichocarpa (32%), Vitis vinifera (12%), Glycine max (5%) and Medicago truncatula (2%; Table 3). Figure 1 Developmental stages of H. perforatum ovules sampled and analyzed in this research. A: Schematic representation of key aspects of sexual and apomictic reproductive pathways in H. perforatum and key developmental stages. B-G: Ovules at developmental stages spanning meiosis, aposporic initial formation and development, and gametogenesis. B: megaspore mother cell (MMC); C: tetrad showing a functional megaspore (fM) along with three degenerating megaspores (dM); D: aposporic initial (Ai) cell; E: enlarged aposporic cell equivalent to an aposporic one-nucleate embryo sac (a1N ES); F: oangic apparatus showing in focus one synergid (Syn) and the egg cell (EC); G: detail of a central cell (CC) from a mature embryo sac. In each photograph the yellow area mark a given germinal cell type (each bar is 8 μm). The reference assembly was built starting from the pool of reads sequenced from each library. Alignment of unigenes on the HAPPY locus and the use of this resource for the identification of allele/splice variants Screening for the identification of putative homologs in the nr database produced a number of matches with proteins encoded by genes located in the BAC clone HM061166.1 (see Additional file 1: Table S3) containing the genetic loci associated with apospory in H. perforatum ( Figure 2, panel A). BLASTN searches of all 60,428 unigenes over the sequence of the BAC clone produced 17 relevant matches, 9 of which aligned to multiple unigenes (see Additional file 1: Table S3). Overall sequence identity shared by unigenes and the corresponding regions of HM061166.1 was on average 95%. Moreover, BLASTN searches did not produce reliable matches for the retrotransposons located in the BAC clone. The fact that the truncated genes ARI-T, HK2 and GH2 did not match none of the unigenes from our collection ( Figure 2, panel A) may indicate that these genes were not expressed or were expressed at levels below our detection threshold. Alternatively, divergence between the genetic loci of the investigated plants (Figure 2, panel A) may have hampered the production of BLAST matches (see also [18]). BLASTX matches from the nr protein database (Additional file 1: Table S3) also revealed possible synteny between the Hypericum sequence HM061166.1 and the chromosomal region located between the loci POPTR_ 0014s16260.1 and POPTR_0014s15970.1 of poplar (Populus trichocarpa). One exception to this was the region containing ARI and a number of other genes, which had truncated forms of ARI-T, NPH3 and TK that are not located in the same chromosome (LG14) in poplar (Additional file 1: Table S3; Figure 2). Positive matches of flower transcripts with nucleotide sequences of HM061166.1 further suggested the presence of some additional coding regions in the BAC clone, including PSP7 and AIR12.
Since distinct unigenes are the product of different assembly output, it is possible that the finding of multiple unigenes matching the same BAC portion reflects the existence of duplicated genes (i.e., paralogs) or allelic variation existing in our pool of genotypes. To test this hypothesis we selected 9 regions of the BAC clone HM061166.1 that shared high similarity with multiple unigenes ( Figure 2; Table 4), aligned the sequences in multiple alignments and analyzed the nucleotide diversity existing between unigenes and reference sequence extracted from HM061166.1. As external controls, the same statistics were made for the three single copy regions ITS or internal transcribed spacers, matK and ndhF (Table 4). Nucleotide diversity displayed by unigenes when compared to their reference sequences extracted from HM061166.1 was an average 0.044 ± 0.062 (within unigenes: 0.025 ± 0.026). Conversely, the nucleotide diversity estimated for the control regions ITS, matK and ndhF was much lower and averaging 0.01 ± 0.01 ( Table 4). The average nucleotide diversity measured in the BAC regions surrounding ARI7-like was as low as 0.013 ± 0.012 (within unigenes: 0.013 ± 0.016). Nucleotide diversity for sequences aligning to the ARI7like gene was equal to 0.170 (within unigenes: 0.080). For a number of unigenes, the nucleotide diversity shared with genes encoding for WRKY-like protein, Statistics are based on multiple unigenes aligning to unique coding sequences predicted from the BAC clone HM061166, containing the HAPPY locus associated to Apospory (AL). For each coding region (Gene product), the Unigene Id, the number of polymorphic sites (S/AL) and measured nucleotide diversity (Pi/AL) are reported. The number of polymorphic sites (S/U) and nucleotide diversity (Pi/U) calculated among unigenes are also indicated. The number of haplotypes emerging from the alignment of the multiple unigenes to coding sequences predicted from the BAC clone HM061166 is indicated as Hn/ALU. **Locus not included in the BAC clone HM061166, which was used as external reference for statistics on sequence genetic diversity. Unigenes were deposited as TSA project under the accession GBXG00000000.
HnRNP, RimL/acetyltransferase-domain protein, PG was equal to 0.00 (Table 4). Despite the low genetic diversity, the unigenes aligned to the genes: Exp1, DGCR-like protein, HnRNP, RimL/acetyltransferase-domain protein, and Mo25-like protein displayed alignment variants featuring a possible lack or insertion of sequence traits in their CDS, coding sequences ( Figure 2, panel B). The distribution of the average number of nucleotide substitutions per site (Dxy) calculated between unigenes and corresponding regions of HM061166.1 showed that nucleotide substitutions were concentrated in the 3'-end of the alignments in at least 50% of investigated regions ( Figure 2, panel C, see also Additional file 2: Figure S1).

The distribution of sequences between single libraries
Of the 60,428 unigenes composing our reference assembly, and identified from different flowers and flower parts, 33,860 sequences were assembled with reads derived from one or multiple libraries. Calculation of the number of raw reads assembled in each unigene from each library allowed us to estimate the presence of each unigene in each sequenced library. These data were used to infer the overlaps existing between the two sequencing reactions (sequencing plates) and independent libraries (i.e., 4 libraries for each sequencing plate). About 92% of the assembled unigenes were composed of sequences shared between the two independent sequencing experiments ( Figure 3, panel A). As much as 86% of unigenes were identified from the flowers of at least three sexual or apomictic individuals ( Figure 3, panel B). Similarly, 78% of unigenes were shared by at least three libraries made from the different flower parts. Taken together these results indicate that most transcripts were detectable at the flowertranscriptome level, regardless of reproductive mode or specific flower organ, while numbers of transcripts detectable in single libraries were low ( Figure 3, panels B-C). The existence and abundance of overlaps between the libraries derived from flowers collected from sexual and obligate apomictic accessions were also measured ( Figure 3, panel B). A total of 552 (1.7% of assembled unigenes) transcripts were present in both apomictic libraries, but were not found in the sexual libraries. Moreover, a total of 673 sequences (2.0% of assembled unigenes) were observed in the two sexual samples, but not in the apomictic samples.
When we considered the different flower parts collected from a facultative apomictic accession (characterized by 24% apospory), and hence showing either apomictic or sexual reproduction, we could identify 616 unigenes (1.9% of total unigenes) exclusive to young flower buds at stages preceding micro-and mega-sporogenesis ( Figure 3, panel C). Moreover, 765 and 1,178 sequences were sequenced only in carpels and stamens, respectively (equal to 2.4% and 3.7% of total unigenes, respectively) ( Figure 3, Figure 3 Statistics on the composition of libraries and distribution of unigenes. Venn diagrams that graphically represent the number of H. perforatum unigenes whose presence was shared among libraries, as indicated by the contribution of each library to the assembly. All possible intersections between libraries, as well as library specific sequences, are shown. A: Sequences shared by the two sequencing approaches; B: Sequences derived from whole flowers of two apomictic (A39, A1973) and two sexual (S13, S36) individuals; C: Sequences derived from single flower parts collected from a facultative apomictic accession. sep|pet: sepals and petals. panel C). The overlap between carpels and stamens consisted of 807 (2.5%) single unigenes. Similarly, 1,195 and 974 transcripts were found in the overlapping regions of young buds with carpels and stamens, respectively.
Finally, most genes residing on the genomic region comprised in the BAC clone HM061166.1, thus surrounding the HAPPY locus, were expressed in the different flower parts as well as in both sexual and apomictic flowers analyzed in this study, as indicated by the production of multiple sequence reads matching the CDS predicted from the genomic sequence HM061166.1 (see Additional file 1: Table S4).

Ontological annotation of sexual and apomictic specific sequences
For the annotation of gene products with a possible key role in plant reproduction and seed formation, we checked the ontological annotation associated to all unigenes identified only in sexual and apomictic libraries (673 and 552 unigenes, respectively; Figure 3). Metrics concerning the full ontological annotation of the flower transcriptome are reported on Additional file 1: Table S5. Most annotations associated to unigenes identified only in sexual and apomictic libraries were shared between the two sequence collections, although some extent of variability was observed for single GO annotations ( Figure 4).
Highest scores were recorded for terms associated to the sensing and response stresses and stimuli as well as terms associated to metabolism of carbohydrate, lipids and secondary compounds (

Identification of genes involved in flower development, gamete formation and plant reproduction
As flower organs were sampled in this study, we expected the libraries to contain genes involved in flower development, gamete formation and reproduction. To test this hypothesis and annotate the unigenes, we created a database of 811 Arabidopsis genes whose function has been associated with plant reproduction sensu lato and used this set of data as a source of data specifically focused on reproduction (see Additional file 1: Table S6). BLAST analyses indicated that 1,674 Hypericum unigenes were putative orthologs to 632 Arabidopsis gene products (78% of total considered genes associated to reproduction in the broad sense).
An attempt to join the annotation of reproductiverelated genes to their expression site was done by checking the distribution of sequences matching reproduction-related genes within the different flower parts. Approximately 63% of the sequences matching reproduction-related genes were shared among all organs. Of the remaining sequences, most were expressed in all flower tissues, with the exception of only the sepals and petals ( Figure 5). To a lesser extent, sequences were present in carpels and young buds and stamens and young buds, followed by stamens and carpels alone ( Figure 5). According to this finding, the distribution of apomicticor sexual-specific unigenes in each plant organ revealed that nearly half of the annotated unigenes belonged to three groups: i) young buds, carpels and stamens; ii) carpels and young buds; and iii) stamens and young buds. Approximately 10% of the apomictic-or sexual-specific unigenes could not be paired with any specific flower part ( Figure 5).
Among the sequences annotated as reproductionrelated, we could effectively annotate numerous unigenes involved in sporogenesis, gametogenesis and embryogenesis (Table 5). NCBI and TAIR searches yielded 89% of the annotations related to meiosis, gametogenesis and embryogenesis (Table 5). Sequences derived exclusively from sexual libraries produced almost double the number of reproductive related genes derived from the apomictic libraries (223 and 118 unigenes, respectively). This difference was particularly evident for genes comprised in the two categories: "regulation of megasporogenesis" (17 and 5 unigenes, respectively) and "female gametogenesis, fertilization and seed development" (21 and 3 unigenes, respectively) ( Table 5).
With this approach, we were able to identify and annotate Hypericum unigenes whose expression has been associated with phases of ovule development (e.g., INO, BEL, ANT), along with transcripts whose activity is restricted or specific to cell fate in the ovule, such as in the nucellar tissue (e.g., AGO9, RDR6 and SGS3) and embryo sac (e.g., ATO, CLO, BLH1 and LIS) (Table 6). Similarly, we could associate 81 unigenes expressed in the Hypericum flower to meiosis (see Table 5). Among those, we detected and annotated a number of genes with known functions in meiosis (e.g., DMC1, ATREC8, MMD1 and MS5), megaspore selection and gamete formation (e.g., AGP18, AGO9, AGO5 and MYB98) ( Table 6). Genes involved in the control of DNA methylation in sporophytic and gametophytic plant tissues, such as MET1 and DME, were also identified and annotated (Table 6). Similarly, 521 and 157 unigenes were linked to gametophyte and gamete development and embryogenesis, respectively (see Table 5). In these categories we could sequence and annotate a number of genes whose mutants are known to affect the embryo sac, embryo and endosperm development (e.g., AGL32, IAA32/ MEE10, dVPE, FUS2, SERK, TANMEI/EMBR2757 and ILA) ( Table 6).

Expression analysis of reproduction-related genes in sexual or apomictic plant accessions
Real-Time RT-qPCR assays were used to verify whether apomictic plants are affected in the expression of genes that are expected to be involved in sexual reproductionrelated processes (Table 6). In particular, the expression of 30 unigenes was tested in pistils and stamens collected from sexual (i.e., meiotic) and apomictic (i.e., apomeiotic) accessions (Table 6; Figure 6, panel A). A sample composed by sepals and petals was also included in the analyses, as external reference.
The expression levels of genes involved in determination of cell and ovule identity was tested in apomictic and sexual pistils in order to investigate differences between meiotic and aposporic gametophyte development. In particular, we studied the expression of key genes involved in the generation and activity of small RNAs and putatively involved in the determination of cell identity in pre-meiotic and meiotic ovules ( Figure 6, panel A). The expression data of HpRDR6 and HpSGS3, two genes involved in the siRNA formation in plants, did not show any differential pattern between apomictic and sexual pistils ( Figure 6, panel A). Noteworthy, HpAGO9, which  The reproductive database was built upon TAIR and NCBI annotations and provides information on genes involved on reproductive processes. For each biological term or process, the number of Hypericum unigenes having a significant match with entries on the reproductive database is shown (unigenes). The number of is implicated in the development and maintenance of MMC identity in the Arabidopsis ovule, was downregulated at early stages and up-regulated at late stages of pistil development in apomictic genotypes compared to sexual ones ( Figure 6, panel A). Some genes potentially involved in ovule and cell identity, like HpINO, did not reveal any differential expression between sexual and apomictic genotypes. Other genes potentially involved in cell identity, including HpANT and HpBEL1, were down-regulated at early and late stages of pistil development in apomictic genotypes ( Figure 6, panel A). The expression data of HpDMC1.2, HpMND and HpMS5 did show that these three genes are down-regulated in apomictic genotypes at early and late stages of pistil development ( Figure 6, panel B). While HpDMC1.2 was differentially expressed between sexual and apomictic  pistils, the related HpDMC1.1 was expressed at comparable levels in apomict and sexual pistils ( Figure 6, panel B). Two Hypericum ARGONAUTE homologs of AtAGO5, both involved in megaspore selection and embryo sac development in Arabidopsis, showed no significant differences in expression levels between apomictic and sexual pistils (Figure 7, panel A). Concerning genes that in Arabidopsis control DNA methylation in sporophytic and gamethophytic tissues, HpDME1 was found upregulated in apomictic genotypes at early and late stages of pistil development, and to a lower extent also HpDME2 was found up-regulated in apomictic genotypes (Figure 7, panel A). In addition, HpMET1 proved to be down-regulated in apomictic genotypes compared to sexual ones at both stages of pistil development (Figure 7, panel A). The expression of several genes that are involved in embryo sac and embryo, and endosperm development ( Table 6) was also tested in the same set of pistils from sexual and apomictic genotypes (Figure 7, panel B). It is worth mentioning that both HpSERK1 and HpSERK2 were differentially expressed in genotypes with antagonist reproductive features, being down-regulated at early and late stages of pistil development in apomictic genotypes (Figure 7, panel B). Moreover, with the only exception of HpdVPE and HpILA, whose expression in pistils appeared to differ in apomictic and sexual genotypes at late developmental stages, most of the tested genes did not reveal expression changes (Figure 7, panel B). The two genes HpMYB98 and HpAGL32, known in Arabidopsis to be specifically expressed in ovule domains including the embryo sac and the endothelium, were found similarly expressed in apomictic and sexual pistils (Figure 7, panels A and B, respectively).

Computational investigation of flower and seed-related genes based on Arabidopsis microarray-based reference transcriptome
The possibility to discover additional genes involved in molecular pathways leading flower and seed development was addressed by comparative approaches focused on A. thaliana flower and seed transcriptomes as references (see Additional file 1: Table S7). To do so we created an Arabidopsis microarray-based reference transcriptome, based on the expression data of 20 published microarray experiments performed in A. thaliana by using flower tissues at developmental stages preceding pollination (e.g., whole flowers, carpels and ovules, anthers and pollen grains). In addition to these, the microarray-based reference transcriptome was enriched with the data of 17 publically available microarray experiments performed on A. thaliana pollinated flowers and seeds (e.g., whole seeds, endosperm, embryo) (Additional file 1: Table S7).
Based on the published data of the A. thaliana microarrays, our reference dataset contained 20,372 genes expressed in at least one of the microarray experiments considered in our study, irrespectively of their expression values (Table 7). This reference dataset was used to set the architecture of pollinated and non-pollinated flowers in MapMan. On the other side, based on our BLASTX results, we could use approximately 60% and 63% of genes expressed in the A. thaliana flower and seed (12,035 and 12,003 hits, respectively) as they produced significant matches with one or multiple Hypericum unigenes (Table 7).
We found that 191 Hypericum transcripts were expressed only before pollination in the Arabidopsis flower (see Additional file 3: Figure S2). Using the same approach, we could detect 65 Hypericum unigenes, which appeared to be expressed upon fertilization in Arabidopsis flowers (Additional file 3: Figure S2, panel A). Interestingly, a number of Hypericum unigenes were shown to be specific to certain seed structures in Arabidopsis (Additional file 3: Figure S2, panel B). This was the case of HpAGL80/FEM111 (AT5G48670) and HpEMB2271 (AT4G21130), whose Arabidopsis putative orthologs appeared to be expressed in the Arabidopsis endosperm.
An overview of metabolic and regulative pathways active during flower development was defined by mapping the Hypericum flower unigenes to the metabolic and regulative environments defined by MapMan (see Additional file 4: Figure S3). Particular emphasis was given to the maps: cell regulation overview and cellular response pathways (Additional file 4: Figure S3). A total of 74 apomictic-specific and 137 sexual-specific gene transcripts, whose expression was restricted to Hypericum flowers, had homologues in the Arabidopsis flower transcriptome (see Additional file 4: Figure S3, indicated as light-blue and green squares, respectively). Sixty-five Hypericum unigenes matched Arabidopsis seed-specific genes (see Additional file 4: Figure S3, red squares) whose annotations were related to the following categories: transcription factors (21 hits; 30%), protein modification (5 hits; 7%) and degradation (10 hits; 14%), receptor kinases (9 hits; 13%), as well as the synthesis and metabolism of hormones (13 hits; 19%; Additional file 4: Figure S3). Among the genes related to seed development in Arabidopsis, the homolog of the B3 family transcription factor AT1G26680 was uniquely present in the sexual libraries in H. perforatum (see Additional file 4: Figure S3, orange squares). Similarly, the expression of the homolog of the embryo-specific zinc-finger transcription factor AT5G0750 (TZF6), which is required for heart-stage embryo formation in the Arabidopsis seed, was present in the apomictic, but not in sexual carpels (see Additional file 4: Figure S3, violet squares).

Discussion
De novo sequencing and assembly of the Hypericum flower transcriptome H. perforatum L. is a medicinal plant with reproductive behavior ranging from highly or nearly obligate apomixis (frequent) to complete sexuality (rare) in natural populations [19,31]. Even though H. perforatum is not a traditional model system for plant biology studies, it is a suitable system for the study of apomeiosis and parthenogenesis ( [17][18][19]26]). To do so, we approached the flower transcriptome with two sequencing efforts conceived for better understanding how and where differential gene expression occurs between both reproductive forms. Hence, we sequenced cDNA collected from single flower organs (i.e., sepals and petals, stamens and carpels) at different stages of flower development, and linked these data to RNAseq data from whole flowers collected from two different obligately-sexual genotypes and two different highly-apomictic genotypes. These efforts led to the assembly of 1.47 million sequence reads to produce 60,428 unigenes, which proved to be a valuable tool for the study of the Hypericum flower transcriptome. Starting from these data, we were able to annotate and characterize 36,988 transcripts found expressed in male and/or female reproductive organs, including tissues or cells of sexual and apomictic flower buds. Our approach of sequencing flowers from two fully obligate sexual genotypes and two unrelated highly apomictic genotypes, in addition to different flower parts dissected from a facultativelyapomictic accession, enabled us to approach the complexity of the flower transcriptome according to its main reproductive organs as well as for alternative reproductive behaviors.
The assembly of such a large number of unigenes provided the possibility of testing for the presence of alternative alleles or splicing variants that could have been sequenced and assembled independently. To verify this thesis we investigated the unigenes aligning to the genomic region comprised in the BAC sequence HM061166.1 and surrounding the HAPPY locus [18]. Nine genes included in the BAC clone aligned to multiple unigenes sequenced from the flower libraries. Despite being sufficiently dissimilar to escape the assembly procedure, the fact that multiple unigenes aligned to the same region could indicate the sequencing of alternative alleles or splicing variants. This could be the case of the BAC regions encoding for the gene products WRKY, DGCR and ARI7, as well as EXP1, RimL and PG. Findings of nucleotide substitutions concentrated in the 3'end of the alignments in at least 50% of investigated regions could be an additional clue that analyzed SNPs are robust and not attributable to sequencing artifacts.
On average, the nucleotide diversity of multiple unigenes mapping to the genomic window surrounding the HAPPY locus [18] was comparable to that estimated for the ITS regions used as external reference [36]. Despite the limitation due to the absence of mapping and quantitative expression data, the finding of nucleotide diversity shared by unigenes and genomic reference stretches comparable to the extent showed by ITS regions suggests that most of the unigenes may resemble allele variants rather than long-lasting duplicated genes (which would have been associated with higher nucleotide diversity, assuming enough time for independent accumulation of mutations). We are aware that coverage provided by the technology used in the study is lower than that recommended for robust variant detection analysis [35], and hence our estimates of nucleotide diversity could be affected by the presence of undetected of sequencing errors. Nevertheless, if we consider that the error rate is dependent on the sequencing method, its distribution across all loci of the BAC clone would be substantially uniform. Hence, we believe that our finding that unigenes aligning on ARI7-like displayed a nucleotide diversity largely exceeding that calculated on the surrounding regions of the BAC clone (0.013) is worth mentioning. This finding is interesting as we consider that ARI7 contains the actual marker co-segregating with apospory in H. perforatum and is among those few genes predicted from HM061166.1 (Additional file 1: Table S3).
Finally, taking into account the low genetic diversity existing within unigenes and CDS predicted from the BAC clone HM061166.1, the finding of alignment variants displaying the insertion or deletion of gene sequence traits in the unigenes provides a first indication for the presence of splice variants in sequences encoded by genes surrounding the HAPPY locus ( Figure 2, see also Additional file 4: Figure S3). The allelic nature of these unigenes will be eventually confirmed by the availability of mapping data.

Genes related to plant reproductive organs and seed formation
De novo sequencing of the H. perforatum flower transcriptome was attempted to identify genes related to plant reproduction, including transcripts specifically or preferentially expressed in anthers and/or pistils, and possibly differentially expressed between sexual and asexual meiosis and gametogenesis. Computational in silico annotation of flower transcripts provided us with an insight into the molecular mechanisms of various biological processes, including meiosis and gametogenesis.
Particular attention was paid to the annotation of genes important for cell specification [3,12] and genes whose mutants affect gametophyte development [5,8,11]. The orthologs of genes whose products are possibly involved in restricting cell-fate in ovules or gametophytes were detected (Table 6; Figure 6). Genes that are involved in regulation of the correct number of cell divisions occurring during megagametogenesis (RBR) [37] or the correct positioning of nuclei within the embryo sac (BLH1/eostre) [38] were also detected in our datasets. Accordingly, transcripts whose expression are thought to be crucial for selection of the functional megaspore and its cell identity (AGP18) [39], or those involved in proper specification of gametes and cell fates, including LIS [7], CLO [40] and ATO [40], were sequenced and annotated.
Overall, these findings demonstrated that our approach could successfully identify gene products whose expression is associated with ovule and seed development, and restricted to a few number of cells [41,42]. For example, we were able to identify the homolog of MYB98 (Table 6; Figure 7), which in Arabidopsis is expected predominantly in the synergid cells and is considered to be critical for synergid differentiation in this species [41]. It is remarkable that HpMYB98, a gene associated with proper differentiation of synergid cells [3][4][5]17], was characterized by high levels of expression variation (compared to sexuals) in the pistils collected at late developmental stages from apomictic accessions (Table 6; Figure 7). As the sexuals and apomicts compared here were of identical ploidy, elevated gene expression variation in apomicts could be consistent with a number of factors including genetic background differences between genotypes [29], trans-acting regulatory variation in the hybrids or genomic differences having arisen due to sexuality versus apomixis [43]. Assuming that PCR efficiency was comparable in the different tissues, the high level of variation recorded in apomictic pistils at stages of late gametogenesis could be the result of spatial or temporal mis-expression of some components of the genetic pathways acting during gametogenesis.
The orthologs of genes that were previously associated with seed and endosperm abortion and anomalies in zygote development, including IAA32/MEE10, dVPE, ILITHYIA, SERK1 and SERK2 among others [4,44], were sequenced and studied in greater details by RT-qPCR. Quantitative expression studies demonstrated that apomictic and sexual pistils have different relative abundance of transcripts encoded by these genes (Table 6; Figure 7). The finding that multiple genes that are involved in seed and endosperm development are differentially expressed between sexual and apomictic pistils implies that entire reproductive gene pathways are modulated in their timing and levels during apomictic development.
Validation using RT-qPCR assays confirmed that HpMET1 [45] and HpDME [46] are differentially expressed among the different flower parts (Figure 7) while only HpMET1 appeared to be differentially expressed in apomictic and sexual pistils at developmental stages corresponding to gametogenesis. In Arabidopsis MET1 absence leads to removal of the silencing methylation marks and the expression of genes (e.g., FIS2 and FWA) involved in the regulation of endosperm development [5]. Accordingly, [45] reported that MET1 is implicated in the repression of endosperm development in the absence of fertilization in Arabidopsis [45], while [47] provided data in support of the idea that a DNA methylation pathway in maize likely plays a critical role in the differentiation between apomictic and sexual reproduction.
Hence, the reduction in the expression of HpMET1 in apomictic pistils further support that variations of DNA methylation marks might be linked to gametophyte development in apomictic H. perforatum.

Identification and expression analysis of candidate genes for apomixis
Apomixis, a form of asexual seed production is characterized by i) meiotically-unreduced egg cell formation (apomeiosis); ii) development of an embryo without the fertilization of the egg cell (parthenogenesis); and iii) endosperm development with (pseudogamous endosperm) or without (autonomous endosperm) fertilization of the central cell [48].
The most abundant GO terms found associated with the unigenes differentially expressed between sexual and apomictic accessions were those associated with transport, response to stress, biotic and abiotic stimuli, and DNA metabolic process and DNA binding ( Figure 4). The number of enriched GO terms specifically related to plant reproduction (i.e., meiosis, gametogenesis, and embryonic and post-embryonic development) was relatively low in either sexual or apomictic genotypes ( Figure 4). Nevertheless, our findings that unigenes found only in sexual or apomictic libraries were annotated as cell cycle, cell growth, cell differentiation, as well as embryonic and post-embryonic development ( Figure 4) could provide some additional indications supporting the hypothesis that apomixis relies upon spatial or temporal mis-expression of genes acting during female sexual reproduction [27,30,[49][50][51][52]. Hence a better understanding of the genetic mechanisms underpinning the switch from sexual to apomictic reproductive strategies could be greatly facilitated by the study of genes primarily involved in the normal progression of sporogenesis, gametogenesis and embryo development. In light of this, we examined whether apomictic seed development was characterized by expression by genes known to be important for the regular progression of gametogenesis and seed development in sexual plants.
One critical finding was the observation that gene products involved in cell differentiation and cell growth, as well as embryonic and post-embryonic development were overrepresented in the two sexual libraries (Figure 4). A closer expression analysis of multiple genes involved in meiosis (HpDMC1, HpMS5, HpMND) or megaspore selection (HpAGP18) indicates a relative decrease in mRNA abundance in apomictic pistils ( Figure 6). The analysis of two distinct HpDMC1-like unigenes indicated that their respective mRNAs had contrasting expression patterns, and while HpDMC1b shows clear differential expression in all considered developmental stages of the pistil, the expression of HpDMC1a is similarly expressed in both apomictic and sexual pistils ( Figure 6). Since cytological and cytometric data [19,29] indicate that apomictic plants in H. perforatum are characterized by a low occurrence of reduced embryo sacs, the decreased expression of critical genes for normal progression of meiosis supports the hypothesis that apospory in this species could be associated to a low rate of successful meiosis.
It has been hypothesized that the molecular switch from sex to apomixis is associated with gene-specific silencing mechanisms associated with chromatin remodeling factors or trans-acting and heterochromatic interfering RNAs involved in both transcriptional and post-transcriptional gene regulation [27]. Recently, a specific route for cell specification and embryo sac development was proposed to occur through the action of different ARGONAUTE (AGO9 and AGO5) genes in Arabidopsis [13,14] and maize [15]. Similarly, mutations in RDR6 or SGS3, two genes involved in the small RNA pathway, are known to result in a relaxation of gametic cell identity and fate in the ovule, leading to changes in cell fate of somatic initials in the nucellar tissue, which differentiate into gametic cells without undergoing meiosis and which can furthermore initiate female gametogenesis through the activation of TEs [14]. The RT-qPCR analyses performed here did not demonstrate differences in the abundance of RDR6 and SGS3 in sexual and apomictic pistils collected at developmental stages spanning female meiosis and gametogenesis ( Figure 6). Similarly, no significant differences were detected in the expression of HpAGO5 in the same plant tissues (Figure 7). Alternatively, since RT-qPCR analyses were performed on whole pistils, we cannot exclude the possibility that small expression differences consistent with gene expression in specific cell types of the ovule might have been lost. This could to be the case of HpAGO5, whose expression in Arabidopsis is expected in the nucellus, where it activates a non-cell autonomous mechanism by promoting the initiation of gametogenesis in the functional megaspore.
Our RT-qPCR data indicated that mRNA levels of HpAGO9 are significantly decreased in apomictic pistils at the earlier developmental stages ( Figure 6). Reduced expression of HpAGO9 in pistils retaining a high level of apospory is noteworthy since AGO9 is involved in restricting the acquisition of gametophyte cell fate in the sub-epidermal layer of the ovule nucellus in Arabidopsis [14], and the phenotype documented for ago9 Arabidopsis plants is remarkably similar to the aposporic gametophyte development [14].
Such considerations of the expression and activity of sRNAs and non-coding RNAs would support the idea that the coding fraction of the transcriptome could be only one side of the coin, and that small and non-coding RNA sequences could represent a meaningful component yet to be fully understood in the frame of gametes and seeds development.

Conclusions
The availability of NGS technologies is raising the ease at which complex phenotypes and processes in nonmodel organisms can be analyzed as a consequence of the increased of throughput and availability of reference DNA and RNA datasets. In our study we were able to annotate and characterize 36,988 transcripts found expressed in male and/or female reproductive organs, including tissues or cells of sexual and apomictic flower buds. Our approach of sequencing whole flowers at different developmental stages from two fully obligate sexual genotypes and two unrelated highly apomictic genotypes, in addition to different flower parts dissected from a facultatively apomictic accession, enabled us to analyze the complexity of the flower transcriptome according to its main reproductive organs (i.e., sepals, petals, stamens and carpels) as well as for alternative reproductive behaviors (i.e., sexual vs. apomictic).
Our findings that unigenes found only in sexual or apomictic libraries were annotated as cell cycle, cell growth, cell differentiation, as well as embryonic and post-embryonic development provided additional indications supporting the hypothesis that apomixis relies upon spatial or temporal mis-expression of genes acting during female sexual reproduction. This hypothesis is further strengthened by our RT-qPCR data, demonstrating that genes expressed in sporophytic and gametophytic cell types and primarily involved in the normal progression of sporogenesis, gametogenesis and embryo development are differentially expressed between apomictic and sexual pistils.
Overall, the results collected in this research represent a critical step toward a better understanding of the genetic and molecular mechanisms leading to gametophyte and gamete determination and development in our model species, in either sexual or apomictic plant genotypes. Data presented here pave the way for whole transcriptome studies aimed at the identification and definition of transcriptome changes associated with the development of male and female reproductive organs by giving particular emphasis to meiosis, the formation of gametes and, more generally, to the processes associated with plant reproduction and seed formation in H. perforatum.

Plant materials
Hypericum perforatum L. plants from two naturally occurring tetraploids (2n = 4× = 32) and three induced tetraploids (2n = 4× = 32) were used for the sequencing approaches (Table 1). For the production of induced tetraploids, plants of the diploid sexual line R1, reselected from the tetraploid apomictic cultivar 'Topaz', were converted to auto-polyploids by colchicine application, as reported by Schallau et al. [18]. Briefly, seeds were imbibed in water for 24 h, placed on filter paper soaked with an aqueous solution of 0.2% colchicine for 24 h and then planted in soil for germination. The C0 plants that survived this treatment were self-pollinated and their progenies (C1) were screened for tetraploid plants. Sexual tetraploid C1 and C2 plants were used for further crosses with tetraploid apomictic pollinators and their progenies were then screened for the reproductive phenotype [18,29]. The reproductive mode of all H. perforatum accessions was estimated by flow cytometric screening of 48 single seeds as indicted by Matzk et al. [31].
We constructed a total of 8 different cDNA libraries. A single cDNA library was obtained from whole flowers collected across developmental stages 1-12 for each of the following accessions: HP13EU and HP36EU (sexual plant accessions), HP39EU and HP1973US (apomictic plant accessions). These four cDNA libraries were sequenced twice. An organ-specific cDNA library was also produced from the facultative apomictic accession HP4/ 13 for each of the following flower parts: buds (whole buds, length <3.0 mm, corresponding to Arabidopsis flower stages 1-10), carpels (bud length >3.0 mm, corresponding to Arabidopsis flower developmental stages [11][12], stamens (bud length >3.0 mm, corresponding to Arabidopsis flower developmental stages 11-12) and sepals/petals (bud length >3.0 mm, corresponding to Arabidopsis flower developmental stages [11][12] [18,53]. These four cDNA libraries were sequenced once.

cDNA synthesis, normalization and pyrosequencing
Total RNA was extracted from whole flowers and flower parts by using the Spectrum™ Plant Total RNA Kit (Sigma-Aldrich) following the protocol provided by the manufacturer. The eventual contamination of genomic DNA was avoided by the optional DNase I (Sigma-Aldrich) treatment. Poly(A) + mRNA was isolated from total RNA using the Ambion MicroPoly(A) Purist Kit according to the manufacturer's instructions (Life Technologies, Darmstadt, Germany) and the integrity and quantity was verified using an Agilent 2100 Bioanalyser and RNA Nanochips (Agilent Technologies, Santa Clara, USA). In order to avoid over representation of the most commonly transcribed genes and to maximize sequence diversity, normalized, full-length enriched cDNA libraries were generated using a combination of the SMART [54] cDNA library construction kit (Clontech, Takara, Saint-Germainen-Laye, France) and the DSN normalization method [55] implemented in the Trimmer Direct cDNA normalization kit (Evrogen, BioCat, Heidelberg, Germany). The procedure generally followed the manufacturer's protocol but included several important modifications, essentially as previously described in more detail [56]. Optimization of the complete cDNA normalization procedure, such as the number of thermocycles for ds-cDNA synthesis, was essentially carried out as described by Vogel and Wheat [57]. Each of the resulting ds-cDNA pools was purified and concentrated using the DNA Clean and Concentrator kit (Zymogen) and size fractionated with SizeSep 400 spun columns (GE Healthcare) that resulted in a cutoff at~200 bp. To test for normalization efficiency, a fraction of the full-length-enriched cDNAs were cut with SfiI and ligated to pDNR-Lib plasmid (Clontech). Ligations were transformed into E. coli ELECTROMAX DH5α-E electrocompetent cells (Invitrogen). For each cDNA library, appr. 200 bacterial colonies were grown in 96 deep-well plates, plasmid mini-preparations were performed using the 96well robot plasmid isolation kit (NextTec) on a Tecan Evo Freedom 150 robotic platform (Tecan) and Singlepass sequencing of the 5' termini of cDNA libraries was carried out on an ABI 3730 xl automatic DNA sequencer (PE Applied Biosystems). Vector clipping, quality trimming and sequence assembly using stringent conditions (e.g., high quality sequence trimming parameters, 96% sequence identity cutoff, 35bp overlap) was done with the Lasergene software package (DNAStar Inc.). For direct sequencing, approximately 1 μg of each SfiI-digested normalized cDNA sample was sheared via nebulization into small fragments. The eight shotgun cDNA libraries were grouped in two plates and sequenced on the Roche 454 FLX platform using Titanium chemistry (Roche Diagnostics Corporation, Basel, Switzerland).

Bioinformatics -EST processing protocol
All high quality reads generated from the two sequencing reactions were assembled in a single reference transcriptome. The assembly was done with gsAssembler (Newbler v2.7, Roche Diagnostics Corporation, Basel, Switzerland) by considering the default parameters, including a minimum read length: 20; seed length: 16; seed step: 12; minimum overlap length: 40 and minimum overlap identity: 90. Both contigs and singletons originated from this procedure were then used as input files in a second assembly step in which all parameters were maintained equal to the previous assembly, except that the minimum overlap length was lowered to 20 bp.
For each single library and assembled sequence, the relative abundance of predicted unigenes was calculated by counting the number of raw reads taking part in the assembly. A unigene was considered expressed in one library if at least one sequence derived form the specified library was included in the assembly of the considered unigene. Transcriptome overlaps and compositions were displayed with Venn diagrams using the web tool venny (bioinfogp.cnb.csic.es/tools/venny/index.html; [58]).
A BLASTX-based approach was used to compare the Hypericum sequences to the nr database and to annotate the assembled Hypericum unigenes, using the BLAST v2.2.29+ downloaded from NCBI, National Center for Biotechnology Information (http://www.ncbi.nlm.nih.gov/).
The H. perforatum clone BAC 25H09 genomic sequence containing the apospory-linked marker and covering the apospory-associated region (GenBank accession number HM061166.1) was previously identified and annotated for several predicted genes, including a variety of transcription factors and retrotransposons [18]. Alignments of unigenes to the BAC sequence HM061166.1 were performed with software the CLC Genomics Workbench v7 (QIAGEN). Statistics on genetic diversity and distribution of the average number of nucleotide substitutions per site (Dxy) were performed with the software DNAsp v5.10.1 [59]. Values of Dxy were extracted from the package of statistics: DNA divergence between populations (sliding window: 100 bp; overlaps: 25 bp).

Plant reproduction database
The plant reproduction database was generated based on the refseq protein sequence repository at GenBank that could be downloaded by browsing the dataset with opportune key words such as ("embryo defective"[All Fields], "maternal effect embryo arrest"[All Fields], "unfertilized embryo sac"[All Fields], "gametophytic factor"[All Fields]). Searches were restricted to the Plants by using the option "Viridiplantae" [Organism]. In a parallel approach, all sequences annotated as "meiosis", "gametophyte development", and "embryogenesis" were downloaded from TAIR (http://www.arabidopsis.org/). Finally, a number of protein sequences whose gene products were studied and linked to "cell specification", "meiosis", "gametogenesis" and "seed development" [3][4][5][10][11][12] were downloaded from Genbank and added to the previous set. On the whole, the plant reproduction database considered 811 non-redundant Arabidopsis gene loci.

Validation of sequencing data by quantitative Real-Time RT-PCRs
Plant materials were selected according to the genetic and cyto-histological bases of apospory recently described for Hypericum perforatum [18,19]. The reproductive mode of all H. perforatum accessions was estimated by flow cytometric screening of 48 single seeds as indicted by Matzk et al. [31]. Samples were collected in 3 biological replicates, from different plant accessions (Table 1). RNA extractions were conducted using the Invisorb Spin Plant RNA Mini Kit (Sigma-Aldrich). cDNA synthesis was conducted using the SuperScript® VILO™ cDNA Synthesis Kit (life technologies), following the indication of the supplier. Primers used in the quatitative RT-PCR experiments are reported on Additional file 1: Table S8. Expression analyses were performed using the thermal cyclers StepOne and 7300 Real-Time PCR System (Applied Biosystem), equipped with 96-and 384-well plate systems, respectively, with the SYBR green PCR Master Mix reagent (Applied Biosystem). The amplification efficiency was calculated from raw data using the OneStep Analysis software (Life Technologies). Amplification performances expressed as fold change were calculated by the ΔΔCt method using HpTIP4 as housekeeping gene [62]. Error bars indicate the standard deviation observed among the three biological replicates.

Computational investigation of flower and seed-related genes based on Arabidopsis microarray-based data
An Arabidopsis microarray-based reference transcriptome was created using the Arabidopsis-expressed sequences, downloaded from publically available experiments. Microarray data sets were downloaded from the arrayexpress data repository at EBI (http://www.ebi.ac.uk/arrayexpress/ experiments/) and used to infer the most probable Arabidopsis transcriptome to be used as a reference (Additional file 1: Table S7). Probes were considered expressed when present in at least one experiment, considering a p-value lower than 0.05.
MapMan (http://mapman.gabipd.org/web/guest/mapman; [63]) analyses were performed by using the Hypericum dataset properly rearranged as input files. Briefly, the Arabidopsis proteome was downloaded from the TAIR, The Arabidopsis Information Resource database (ftp://ftp.arabidopsis.org/home/tair/Sequences/BLAST_ datasets/TAIR10_BLASTsets/) and set as reference database for local BLASTX analysis performed using the H. perforatum transcriptome dataset as the query. AGI codes relative to Arabidopsis putative homologous genes (E-value cut-off: 1.0E-9) were recovered by each tabular BLASTX result (BLAST-2.2.25+ argument: -outfmt 6) and used to download the corresponding ATH1-121501 genechip identifiers (Affymetrix) from TAIR (http:// www.arabidopsis.org/tools/bulk/microarray/index.jsp) (for more details see [61]). MapMan analyses focused on maps: Regulation overview and Cellular response overview, which were used to graphically display the data.