Deficits in skilled motor and auditory learning in a rat model of Rett syndrome

Rett syndrome is an X-linked neurodevelopmental disorder caused by a mutation in the gene MECP2. Individuals with Rett syndrome display developmental regression at an early age, and develop a range of motor, auditory, cognitive, and social impairments. Several studies have successfully modeled some aspects of dysfunction and Rett syndrome-like phenotypes in transgenic mouse and rat models bearing mutations in the MECP2 gene. Here, we sought to extend these findings and characterize skilled learning, a more complex behavior known to be altered in Rett syndrome. We evaluated the acquisition and performance of auditory and motor function on two complex tasks in heterozygous female Mecp2 rats. Animals were trained to perform a speech discrimination task or a skilled forelimb reaching task. Our results reveal that Mecp2 rats display slower acquisition and reduced performance on an auditory discrimination task than wild-type (WT) littermates. Similarly, Mecp2 rats exhibit impaired learning rates and worse performance on a skilled forelimb motor task compared to WT. Together, these findings illustrate novel deficits in skilled learning consistent with clinical manifestation of Rett syndrome and provide a framework for development of therapeutic strategies to improve these complex behaviors.


Background
Rett syndrome is a rare neurodevelopmental disorder associated with a mutation in the X-linked gene MECP2. This disorder mostly affects females, who display normal early development followed by regression of already acquired skills [4]. Individuals with Rett syndrome exhibit a range of symptoms, including seizures, breathing abnormalities, motor impairments, changes in sensory responses, anxiety, and speech-language deficits [4,14].
The development of interventional strategies to improve function in these domains is of clear clinical importance.
To address this and provide a testbed for evaluation, transgenic mouse and rat models bearing mutations in the MECP2 gene have been developed [12,13,25,31]. Some features of these models have been well characterized and provide substantial insight into the pathophysiology of Rett syndrome. However, testing complex behaviors in Mecp2 rodents is in its early stages. Initial work successfully modeled psychomotor regression similarly seen in girls with Rett syndrome by using a seed opening task in Mecp2 rats [31]. Other studies using the same rat model have found abnormalities in gait, rotarod performance, and speech discrimination performance [3,9]. It is likely that detailed characterization of other complex behaviors known to be altered in Rett syndrome, such as skilled learning, could expand the utility of these animal models. Here, we sought to characterize the acquisition and performance of skilled motor function and to replicate the previous findings characterizing auditory discrimination performance.
To do so, we trained female heterozygous Mecp2 rats and wild-type (WT) littermate control rats to perform a speech discrimination task to assess auditory processing or a skilled forelimb reaching task to evaluate motor function. In both the complex auditory and motor tasks, Mecp2 rats exhibit acquisition of the task and improved performance with continued training. However, the rate of learning, as well as several metrics of performance, are reduced in Mecp2 rats compared to WT, indicative of a deficit in skilled learning. Impaired performance corresponds to dysregulated neural function in auditory and motor networks in transgenic Mecp2 models [9,21]. These results provide a novel characterization of skilled learning in Mecp2 rats and develop a framework for future studies to evaluate the effect of potential interventions on complex behavioral tasks.

Subjects
Twenty-four female Mecp2 heterozygous rats and 18 female wild-type littermates were used in this study. Rats were generated by breeding a Mecp2 heterozygous Sprague-Dawley female with a wild-type Sprague-Dawley male. Female breeders were obtained from Horizon Discovery (SAGE labs), using zinc finger nuclease technology that generated a 71 base pair deletion in exon 4 [3,9,31]. Experimenters were blind to the genotype. Animals underwent behavioral assays and training between 4-8 months of age. Nine Mecp2 rats and 11 wild-type rats underwent auditory discrimination training. Fifteen Mecp2 rats and 13 wild-type rats underwent motor training. Following motor training, 7 of the Mecp2 rats and 9 wild-type littermates underwent intracortical microstimulation (ICMS). All animals were housed in a 12:12 h reversed light-dark cycle and were food-deprived during training. A small subset of animals did not complete all tasks they were assigned to (Additional file 1). Similar to previous studies [9], 18 of the 24 Mecp2 rats had one or more seizures. When seizures occurred, rats were given a 30-min break or removed from the session for the day. All protocols were approved by The University of Texas at Dallas Institutional Animal Care and Use Committee.

Auditory behavioral testing
Speech discrimination tasks and procedures were similar to previous studies [7][8][9]. Training sessions occurred twice a day for 1 h each, 5 days a week. Rats were first trained to press a lever or nose poke for a food reward until they reached a criteria of 100 independent responses for two sessions. Once this criteria was met, rats began training on a sound detection task. Rats were trained to respond to the target speech sound 'dad' within 3 s of sound presentation until they reached the criteria of ≥ 75% correct for 5 sessions, or until they reached 50 sessions of detection. Rats received a 45 mg food pellet reward (45 mg dustless precision pellet, Bio-Serv Frenchtown, NJ) for a correct response to the target sound 'dad', and received a 6-s time out for an incorrect response to silence catch trials (Fig. 1a). Following this detection task, rats were trained on a speech discrimination task, in which they were required to respond to the target sound 'dad' and ignore the non-target sounds 'bad', 'sad', 'deed', and 'dood' (Fig. 1d). Each rat was trained on the discrimination task for 3 weeks. Rats then began training on a speech in noise task in which they were tasked to respond to the target sound 'dad', and ignore non-target sounds ('bad', 'sad', 'deed', and 'dood') during various levels of background noise (0, 48, 54, 60, and 72 dB) with the speech sounds presented at 60 dB (Fig. 1g). Each rat was trained on the speech in noise task for 2 weeks.
All speech sounds were spoken by a female native English speaker, as in previous studies [7][8][9]. Sounds were presented so that the loudest 100 ms of the vowel was 60 dB. The STRAIGHT vocoder was used to frequency shift all speech sounds up by one octave into the rat hearing range, while leaving all temporal information intact [19].

Motor behavioral testing
Rats were trained on a skilled motor task, as in previous studies [15][16][17]23]. The task was an automated lever pressing task, in which the animal was required to learn to reach outside of a cage and press a lever twice in rapid succession. The behavior apparatus consisted of an acrylic cage, with a slot in the front right for access to a lever that was positioned 1 (inside) to 2 cm (outside) away from inside the edge of the chamber [15][16][17]23] (Fig. 2a). Reward pellets were delivered to a receptacle in the front left side of the apparatus. A potentiometer was affixed to the lever to record the angle of the lever relative to the horizontal. The lever allows for up to a 13°d epression. A lever press was defined as a deflection greater than 9.5°, followed by a release to at least 4.75°. A spring supported the lever, providing 28 g of resistance and allowing the lever to return to its horizontal resting position. An electronic controller board sampled the potentiometer position at 100 Hz and relayed the information to MotoTrak software the controlled the task criteria and collected data (MotoTrak, Vulintus, Louisville, CO).
Training sessions were 30 min in duration and occurred twice per day separated by at least 2 h, 5 days per week. Rats were first trained to press a lever that was positioned inside the apparatus. Throughout training, rats progressed through five stages, each requiring different criteria for trial success and consequent reward delivery, as detailed in Table 1. During the final stage, rats were rewarded for double pressing within a 500 ms window (Fig. 2b). Once the final stage was completed, rats underwent intracortical microstimulation (ICMS).

Intracortical microstimulation
The day after the final day of motor training, rats underwent ICMS to evaluate left motor cortex organization contralateral to the trained paw. Rats were anesthetized with ketamine hydrochloride (70 mg/kg, i.p.) and xylazine (5 mg/kg, i.p.), with supplementary doses given as needed to maintain anesthesia levels. Doxapram (20 mg/ kg, i.p.) and glycopyrrolate (0.5 mg/kg, i.p.) were given to stabilize breathing and heart rate as needed. A small incision of the cistern magna was made to attenuate cortical swelling. A craniotomy and durotomy was performed to expose the left motor cortex. A tungsten electrode (0.1-1 MΩ) was inserted into the brain at a depth of 1.75 mm. Stimulation sites were then chosen at random on a grid with sites set 500 μm apart from each other. ICMS stimulation consisted of a 40 ms pulse train of 10 pulses.
ICMS procedures were conducted with two experimenters to ensure blinding to group and electrode location. The first experimenter placed the electrode and recorded data from each site. The second experimenter, blinded to the genotype of the rat and electrode position, delivered stimulations and classified movements. Stimulation was  vs. vs.

Mecp2 WT
Mecp2 WT Fig. 1 Speech discrimination performance is impaired in Mecp2 rats. Target and non-target sounds for the a detection task, d speech discrimination task, and g speech in noise task. b Detection, a simple auditory behavior, was unimpaired in Mecp2 rats compared to WT (Mecp2 n = 9, WT n = 11). However, Mecp2 rats displayed significant impairments in the more challenging tasks: e speech discrimination in quiet (Mecp2 n = 9, WT n = 11) and h speech discrimination in varying levels of background noise (Mecp2 n = 8, WT n = 11). c, f, i Mecp2 rats were significantly slower at responding to the target sound in all tasks. Circles depict individual subjects. Bars represent the mean and error bars indicate SEM across rats. The asterisk (*) denotes p < 0.05 across groups gradually increased from 20 to 250 μA, or until a movement was observed. The stimulation amplitude at which a movement was first seen was documented as the threshold. If no movement was seen at 250 μA, then that site was recorded as no response. Movements were classified as proximal forelimb, distal forelimb, head, or hindlimb. Cortical area was calculated by multiplying the number of sites eliciting a response by the area surrounding a site (0.25 mm 2 ).

Social behavior tests
To assess social behavior, 9 Mecp2 rats and 9 WT littermates underwent a sociability and social novelty preference task. The behavioral arena was a rectangular box 70 cm in length × 30 cm wide × 50 cm depth. The apparatus was divided into three chambers. The two outside chambers each contained a wire cage. The experimental animal was placed inside the middle chamber and allowed to freely explore for 5 min to habituate to the apparatus. To test sociability, an unfamiliar rat that had no prior contact was then placed into one of the wired cages, and the experimental rat freely explored for 10 min. Sociability was defined as the amount of time the experimental rat spent in the chamber with the unfamiliar rat. The sociability index (time spent with the unfamiliar rat − time spent in the empty chamber) ÷ (time spent with the unfamiliar rat + time spent in the empty chamber) was used to indicate a preference to interact with or avoid the rat. To test social novelty, a second unfamiliar rat was placed in the wired cage in the chamber that had previously been empty. The experimental rat was allowed to freely roam for another 10 min. Social novelty was defined as how much time was spent with the novel rat compared to the  Fig. 2 Skilled motor learning is impaired in Mecp2 rats. a Illustration of a rat performing the lever pressing task. Rats were tasked with reaching out through a narrow slot in the cage and pressing a lever twice in rapid succession. b Representative data collected using the task, depicting a trial with a successful double press. c Mecp2 rats were significantly slower to progress though training than WT (Mecp2 n = 15, WT n = 13). d A smaller portion of Mecp2 rats completed the final stage of training within 12 weeks compared to WT rats (Mecp2 n = 15, WT n = 13). e Mecp2 rats demonstrated significant reductions in lever pressing speed, as evidenced by an increase in the inter-press interval (IPI) (Mecp2 n = 10, WT n = 13). f Additionally, Mecp2 rats completed significantly fewer trials than WT animals (Mecp2 n = 15, WT n = 13). Circles depict individual subjects. Bars represent the mean and error bars indicate SEM across rats. The asterisk (*) denotes p < 0.05 across groups now familiar rat. The social novelty index (time spent with the novel rat − time spent with the familiar rat) ÷ (time spent with the novel rat + time spent with the familiar rat) was used to indicate a preference to interact with the novel rat over the familiar rat. The rats used during the sociability and social novelty tests were of the same age and sex as the experimental rats.

Standard behavioral assessments
To assess repetitive behaviors, 9 Mecp2 rats and 9 WT littermates underwent a marble burying test. Rats were habituated to the novel bedding (BioFresh nitrocellulose comfort bedding). Rats were then placed into a new cage with 15 marbles for 10 min. The number of marbles buried was recorded. Nine Mecp2 rats and 9 WT littermates also underwent a spontaneous alternation task. Rats were placed in a plus maze and were allowed to freely explore for 10 min. A spontaneous alternation occurred when a rat entered all arms within five consecutive entries. The percentage of spontaneous alternation was calculated by dividing the number of alternations by the number of possible alternations (number of alternations) ÷ (number of total arm entries − 4) × 100.

Statistical analysis
Statistical analysis was performed with MATLAB software. Speech discrimination performance was measured as percent correct, determined by the average of correct responses to the target speech sound and correct rejections to the non-target speech sounds. Performance on the speech in noise task was taken from the first week of training. The average reaction time to respond to the target sound was also assessed. Two-way repeated measure ANOVAs were used to analyze discrimination performance between experimental groups as well as discrimination performance over time. Post hoc unpaired t tests were used to determine statistical significance. A repeated measure general linear model was used to analyze speech in noise performance between groups and noise levels. Post hoc pairwise comparisons were Bonferroni-corrected for each noise level. Behavioral analysis of motor function included average training stage progression, number of trials per session, and lever inter-press interval (IPI). Significant differences for average stage progression were determined using the Wilcoxon rank-sum test. Mean IPI was calculated from the fourth and fifth stages. Significant differences in behavior measures were determined using unpaired t tests. The chi-square test was used to determine significance in the portion of rats that completed the study. To assess ICMS data, an unpaired t test was used to determine significance in movement representation size, total map size, and average thresholds. Measures for the behavioral assays include the number of marbles buried, sociability, social novelty, and percent alternations. Unpaired t tests were used to determine group differences in behavioral assays. All data is reported as mean +/− standard error of the mean (SEM).

Results
A number of studies document alterations in auditory processing in individuals with Rett syndrome [1,10,18,27,28] and animal models [9,12,20]. We sought to evaluate the acquisition and performance on a speech discrimination task in Mecp2 rats to confirm previous findings [9]. Mecp2 and WT rats were initially trained to respond to presentation of the speech sound 'dad'. Detection of the speech sound, calculated as the percentage of trials in which rats correctly responded to the target sound, did not significantly improve in either groups over the course of 5 weeks of training ( Fig. 1b; two-way repeated measures ANOVA, F(4, 28) = 1.3288, p = 0.238). No differences in performance were observed in between groups on this simple task ( Fig. 1b; two-way repeated measures ANOVA, effect of group; F(1, 7) = 0.025, p = 0.878). A significantly slower reaction time was observed in the Mecp2 group compared to the WT control group (Fig. 1c; Mecp2 2.45 ± 0.07 s, WT 2.13 ± 0.1 s, Unpaired t test, p = 0.029). These findings are consistent with previous studies and suggest that gross auditory processing is generally intact in Mecp2 rodents [9,12,20].
We next assessed performance in these rats on a more challenging auditory task that requires discrimination of the target sound from a number of similar distractor sounds, either in a quiet environment or in the presence of background noise. Mecp2 rats demonstrated significantly impaired performance compared to WT in the absence of background noise over the course of 3 weeks of testing ( Fig. 1e; two-way repeated measures ANOVA, effect of group; F(1, 18) = 5.99, p = 0.025). Post hoc analysis revealed that Mecp2 rats performed significantly worse in week 2 of discrimination training and a trend towards worse performance on weeks 1 and 3 (unpaired t test, week 1: p = 0.054, t = 2.05; week 2: p = 0.0172, t = 2.62; week 3 p = 0.055, t = 2.04). The presence of background noise significantly degraded discrimination performance in both groups ( Fig. 1h;  Consistent with reduced performance, Mecp2 rats exhibited significantly slower reaction times (Fig. 1f, i; unpaired t test, discrimination: Mecp2: 1.82 ± 0.11 s, WT: 1.52 ± 0.04 s, p = 0.013; speech in noise: Mecp2: 1.99 ± 0.08 s, WT: 1.69 ± 0.07 s, p = 0.012). To confirm that impaired performance is not due to motor deficits that slow reaction time, we extended the hit window to 6 s for Mecp2 rats and reanalyzed discrimination performance. Even with this prolonged hit window, Mecp2 rats demonstrated significantly impaired discrimination compared to WT over the course of 3 weeks (6 s hit window: twoway repeated measures ANOVA, effect of group; F(1, 18) = 5.96, p = 0.025). These findings indicate that impaired performance is not likely due to motor deficits that may slow response rate, but rather can be ascribed to incorrect responses. These findings indicate that Mecp2 rats exhibit deficits on a complex auditory task that are not apparent in a simple task.
Previous studies report modest deficits in motor function in Mecp2 heterozygous or KO animal models [3,13,24,26,29,31,32], but these deficits largely fail to replicate the substantial motor dysfunction observed in Rett patients. Given the appearance of deficits on a challenging auditory task, we next sought to characterize acquisition and performance of Mecp2 rats on a skilled forelimb motor task. Mecp2 and WT rats underwent training on a task that requires the rats to reach their forelimb through a narrow slot in the cage and press a lever twice in rapid succession to receive a food reward. The training was staged to become progressively more challenging as performance improved, such that longer reaches and faster presses were required (Table 1). Mecp2 rats demonstrated significantly slower progression through training stages than WT animals over training, indicative of an impairment in skilled motor learning ( Fig. 2c; Wilcoxon rank-sum test, weeks 2-12, p < 0.05). Consequently, a smaller proportion of Mecp2 rats completed the final stage of training within 12 weeks compared to WT (Fig. 2d; Mecp2: 3 of 15 rats, WT: 9 of 13 rats, χ 2 test, χ 2 = 6.89, p = 0.009). Mecp2 rats displayed a significantly slower interval between successive lever presses than WT ( Fig. 2e; Mecp2: 0.86 ± 0.1 s, WT: 0.57 ± 0.04 s, unpaired t test, p = 0.013). Additionally, Mecp2 rats performed fewer trials during behavioral training sessions, consistent with previous reports of motor hypoactivity [3,13,24,26,29,31,32] (Fig. 2f; Mecp2: 114.29 ± 11.27, WT: 144.19 ± 8.61, unpaired t test, p = 0.049). Together, these results indicate an impairment in skilled motor function in Mecp2 rats.
We next sought to explore whether large scale changes in cortical circuits could explain motor performance deficits in Mecp2 rats. To do so, we conducted ICMS mapping to document movement representations in the motor cortex after the conclusion of motor training. No differences in overall area of the motor cortex or the size of individual movement representations were observed between Mecp2 and WT rats (Fig. 3a, unpaired t , unpaired t test, p = 0.3). The absence of gross changes in cortical movement representations suggests that motor dysfunction may arise from changes in synaptic function in motor networks, rather than large-scale changes in corticospinal connectivity.

Discussion
The present study provides a novel characterization of complex skill learning in Mecp2 rats. We report that heterozygous Mecp2 rats display slower acquisition and reduced performance on an auditory discrimination task than WT littermates. Similarly, Mecp2 rats exhibit impaired learning rates and worse performance on a skilled forelimb motor task compared to WT. Together, these findings illustrate skilled performance deficits that are novel and consistent with clinical manifestation.
We find that Mecp2 rats were impaired during a more challenging auditory task, corroborating a previous report [9]. These findings are consistent with clinical evidence that while individuals with Rett syndrome generally have normal gross auditory processing, higher levels of auditory processing are often degraded [1,9,12,18,20,27,28]. This is also supported by evidence of higher hearing thresholds and decreased response strength to low frequency sounds in the primary auditory cortex of Mecp2 rats [9]. Additionally, we found that Mecp2 rats were slower at responding to the target sound on all speech discrimination tasks. Both individuals with Rett syndrome and Mecp2 animal models exhibit delayed cortical responses to auditory stimuli [1,9,10,12,20]. This degraded auditory processing likely contributes to the slower reaction times to auditory stimuli observed in the current study.
Given that Mecp2 rats were impaired during a more challenging auditory task, we sought to characterize performance on a skilled forelimb motor task. Overall, Mecp2 rats had a significant impairment in skilled motor function compared to WT controls. In the current study, the reduced forelimb movement speed, as assessed by longer latencies between presses on the task, are consistent with deficits in motor performance in Mecp2 rats [5,31]. Additionally, Mecp2 rats initiated fewer trials during behavioral testing sessions, consistent with motor hypoactivity previously documented with the open field and rotarod tasks [3,13,24,26,29,31,32]. The impairments in motor learning and skilled motor function reported here are consistent with clinical observations. Moreover, these findings expand the utility of the transgenic rat model and suggest that the model may be useful as a framework to evaluate interventions aimed at improving motor function.
Studies have documented impaired motor cortex plasticity in both Mecp2 rodent models [21,22] and Rett syndrome patients [2], which may contribute to motor dysfunction. Based on this data and our observation of deficits in skilled motor function, we sought to explore whether large-scale changes in cortical circuits could explain poor motor performance in Mecp2 rats. However, no differences in the cortical movement representation area or stimulation threshold to evoke movements were observed. The technique used to evaluate motor function in this study, intracortical microstimulation, largely provides a coarse evaluation of direct corticospinally evoked movements. Similar to the auditory system, our findings indicate that motor circuits are largely preserved in Mecp2 heterozygous rats, and the motor  Fig. 4 Mecp2 rats display some cognitive impairments. a The number of marbles buried was not significantly different between Mecp2 rats and WT. b Mecp2 rats exhibit significantly fewer spontaneous alternations compared to WT. c No difference in the number of entries was observed. d, e Additionally, no difference in sociability or social novelty was observed between groups. Circles depict individual subjects (WT n = 9, Mecp2 n = 9). Bars represent the mean and error bars indicate SEM across rats. The asterisk (*) denotes p < 0.05 across groups dysfunction likely reflects changes in synaptic plasticity or dysfunction in higher processing areas. Future studies directed at examining finer-scale synaptic changes in motor networks may provide more insight into skilled motor dysfunction. Along with severe auditory and motor dysfunction, Rett syndrome patients exhibit cognitive and social deficits. This has been modeled in various rodent models of Rett syndrome [6,11,26,[30][31][32]. We sought to corroborate some of these findings in our Mecp2 female rat model. To do so, Mecp2 and WT rats underwent a social interaction task, a marble burying task, and a spontaneous alternation task to assess sociability and social preference, repetitive behaviors, and working memory, respectively. While we did not observe differences in social or repetitive behaviors, we did observe a working memory impairment, indicated by a reduction in percent alternation in the spontaneous alternation task. This finding is consistent with a previous study that observed working memory impairments in a novel Mecp2 male mouse model [11]. Conversely, we did not observe abnormal social behavior or repetitive movements. This difference may arise from the fact that female Mecp2 rodents used in the present study exhibit a less severe phenotype compared to male rodents [24,26]. Additionally, some studies have suggested that social behavior may improve over time in Rett syndrome patients [33], which may explain the lack of abnormal social behavior in the current study. A previous study documented abnormal social behavior in juvenile female Mecp2 rats [31], while the current study used adult Mecp2 rats. Future studies evaluating social behavior at several timepoints may provide more insight to the manifestation and time course of abnormal social behavior in Mecp2 rats.
Several studies have successfully modeled MECP2 dysfunction and Rett syndrome-like phenotypes in Mecp2 mice [12, 13, 20-22, 26, 29]. The Mecp2 rat model used in the present study provides the opportunity for more complex behavioral analysis that may offer more insight into the pathology of Rett syndrome. Additionally, our experiments were conducted in adult Mecp2 heterozygous females, which is more analogous to heterozygous mutations found in girls with Rett syndrome. A recent preceding study highlights the utility of the female Mecp2 rat model, using the model to identify psychomotor regression similarly seen in Rett syndrome girls [31].
A number of limitations of this study merit consideration. While the presented data provides initial evidence of skilled learning deficits in auditory and motor tasks in Mecp2 rats, rodent models fail to capture the complexity of skilled learning in humans. Future studies that expand evaluation to a broader range of tasks will provide a more comprehensive assessment of skilled learning in this model. In the current study, we observed significantly slower reaction times in Mecp2 rats, consistent with reduced performance on the speech discrimination task. Based on the present data, we cannot exclude the possibility that slower reaction time arise from abnormal motor function. However, given that Mecp2 rodents have generally intact motor function but abnormal auditory cortical responses [9,12,20], we believe that the speech discrimination impairment is due to atypical cortical auditory processing. Future studies should incorporate recording of neural responses in the brainstem and the auditory cortex in conjunction with behavioral discrimination to examine the contribution of auditory processing. One of the central findings of the present study is the observation of impaired motor learning and performance on a skilled task. The current study also found that Mecp2 rats performed fewer trials during motor behavioral training sessions, which is consistent with previous reports of motor hypoactivity with the open field and rotarod tasks [3,13,24,26,29,31,32]. It is possible that impaired motor skills could arise from lack of motivation or general muscle weakness. Preliminary results from a previous study indicate potential muscle weakness in Mecp2 females, but the magnitude of this effect is unclear [24]. Moreover, recent evidence found that impairments in motor cortex plasticity correlates with motor deficit in individuals with Rett syndrome, suggesting that central changes contribute to motor dysfunction [2]. Future studies are needed to delineate the contributions of central and peripheral mechanisms on motor dysfunction.

Conclusions
The development of interventional strategies to improve the quality of life of individuals with Rett syndrome is of clinical importance. Characterization of more complex behaviors known to be altered in Rett syndrome could expand the utility of animal models. The current study characterizes skilled motor learning, replicates previous work evaluating auditory discrimination performance in female Mecp2 rats, and establishes a framework for future studies to evaluate the effect of potential interventions on complex behavioral tasks.
Additional file 1. Task assignment for each animal.