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Rapid Method for Screening of Immunoglobulins in Porcine Fetuses, Using Rocket Immunoelectrophoresis

Application of an Interspecies Reaction Between Human and Porcine μ-Chain

Raket immunelektroforese som en hurtig metode til “screening” af immunglobuliner i grisefostre. Anvendelse af en interspecies reaktion mellem human og porcin μ-kæde

Acta Veterinaria Scandinavica volume 20, pages 313–320 (1979)Cite this article

Abstract

A method for screening of immunoglobulin levels in porcine fetuses is presented. The method is based on rocket electrophoresis. This method is sensitive, but when used for assaying immunoglobulins it is only semiquantitative. It was shown that sera from 11 hysterectomy-derived colostrum-deprived neonatal pigs from a normal gilt contained no IgM or IgA, but small amounts of IgG. Sera from 9 piglets isolated in the same way from a gilt infected with porcine parvovirus contained all 3 antibody classes. IgM was detected by an interspecies reaction using anti human μ-chain immunoglobulin.

Sammendrag

En metode til “screening” af immunglobulinniveau i grisefostre fremlægges. Metoden er baseret på raketelektroforese. Denne metode er følsom, men kun semikvantitativ ved bestemmelse af immunglobuliner. Det påvistes, at sera fra 11 grise, som blev taget ved hysterektomi, og som ikke fik kolostrum, indeholdt små mængder IgG, men ikke IgM eller IgA. Sera fra 9 tilsvarende grise, som blev taget fra en gylt der var inficeret med porcint parvovirus, indeholdt alle 3 antistofklasser. IgM blev påvist ved en interspecies reaktion under anvendelse af anti human μ-kæde Immunglobulin.

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Authors and Affiliations

  1. State Veterinary Institute for Virus Research, DK-4771, Lindholm, Kalvehave, Denmark

    K. Dalsgaard, E. Overby, J. J. Metzger & A. Basse

  2. I.N.R.A. Research Station for Virology and Immunology, Thiverval-Grignon, France

    K. Dalsgaard, E. Overby, J. J. Metzger & A. Basse

  3. Department of Pathology, Royal Veterinary and Agricultural University, Copenhagen, Denmark

    K. Dalsgaard, E. Overby, J. J. Metzger & A. Basse

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  1. K. Dalsgaard
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  2. E. Overby
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  3. J. J. Metzger
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  4. A. Basse
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This work was partly supported by the Commission of the European Communities (Swine fever programme).

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Dalsgaard, K., Overby, E., Metzger, J.J. et al. Rapid Method for Screening of Immunoglobulins in Porcine Fetuses, Using Rocket Immunoelectrophoresis. Acta Vet Scand 20, 313–320 (1979). https://doi.org/10.1186/BF03546593

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Acta Veterinaria Scandinavica

ISSN: 1751-0147