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Possible Application of the IHI Test in the Species Identification of Hair
Acta Veterinaria Scandinavica volume 13, pages 266–268 (1972)
Abstract
Immunological species differentiation of keratein from hair has previously been demonstrated by the precipitin ring test in tubes (Pillemer et al. 1939) and by the indirect hemagglutination test (Simonsen 1970). In the present study the possibility of species identification of s-carboxymethylkeratein (SGMK) from single hairs was examined. SCMK and rabbit anti-SCMK sera from man, horse, dog and ox were prepared according to methods described by Gillespie (1962) and Simonsen (1970, 1971). Suitable antisera were used for the indirect hemagglutination-inhibition (IHI) test (Stavitsky 1954). The antisera were absorbed with heterologous SCMK and the inhibition test performed using SCMK extracted from 5 cm stretches of hairs by reduction and alkylation in 1 ml fluid volumes. To each vial containing 0.5 ml of antiserum in a serial 2-fold dilution row of the respective antisera was added 0.05 ml of a homologous or a heterologous single-hair SCMK. After incubation at 37 °C for 30 min. SCMK-coated goat erythrocytes were added and the test read after incubation at 20 °C for 18 hrs.
References
Gillespie, J. M.: The isolation of some soluble proteins from wool. Aust. J. biol. Sci. 1962, 15, 262–277.
Pillemer, L., E. E. Ecker & J. R. Wells: The specificity of keratins. J. exp. Med. 1939, 69, 191–197.
Simonsen, H. B.: Differentiation of keratein from bovine and canine hair by passive hemagglutination. Acta vet. scand. 1970, 11, 664–605.
Simonsen, H. B.: Disc electrophoretic studies on an S-carboxymethyl- kerateine preparation from bovine and canine hair. Acta vet. scand. 1971, 12, 307–309.
Stavitsky, A. B.: Micromethods for the study of proteins and antibodies. J. Immunol. 1954, 72, 360–375.
Acknowledgments
The author is grateful to Bente Thorsen for skilled technical assistance.
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Simonsen, H.B. Possible Application of the IHI Test in the Species Identification of Hair. Acta Vet Scand 13, 266–268 (1972). https://doi.org/10.1186/BF03548582
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DOI: https://doi.org/10.1186/BF03548582