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Occurrence of the blaZ Gene in Penicillin Resistant Staphylococcus aureus Isolated from Bovine Mastitis in Denmark

Forekomst af blaZ genet i penicillinresistente Staphylococcus aureus isoleret fra bovin mastitis i Danmark

Acta Veterinaria Scandinavica volume 40, pages 279–286 (1999)Cite this article

Abstract

Fifty-eight penicillin resistant Staphylococcus aureus isolates of known phage types from bovine mastitis in Denmark from the 1950’ties (10 isolates) and the 1990’ties (48 isolates) were tested for beta-lactamase production. Furthermore, the presence of blaZ and blaR1 and the location of blaZ was determined by PCR and hybridisation. All isolates produced beta-lactamase and contained blaZ and blaR1. The blaZ gene was located on the chromosome in 54 isolates and on plasmids of different sizes in 4 isolates. Sequence analysis of an internal region of blaZ in 2 isolates of bovine origin showed a high degree of homology to already published sequences from human isolates. BlaZ could be transferred from the 4 isolates with plasmid location whereas it was not possible to transfer blaZ from 3 isolates with chromosomal location of the gene. The blaZ gene and the blaR1 gene were located closely to each other as previously published. In contrast to observations among isolates of human origin, no correlation between penicillin resistance and phage pattern was indicated for the bovine isolates. Furthermore, in contrast to the observed shift towards increased occurrence of plasmid location of blaZ among isolates of human origin in Denmark, blaZ appears to remain predominately chromosomal located among isolates of bovine origin.

Sammendrag

Otteoghalvtreds penicillinresistente Staphylococcus aureus isolater med kendte fagtyper fra mastitis hos kvæg, som var isoleret i 1950erne (10 isolater) og 1990erne (48 isolater), blev undersøgt for beta-laktamaseproduktion, forekomst af blaZ og blaR1, samt lokalisering af blaZ. Alle isolater producerede betalaktamase og indeholdt blaZ og blaR1. BlaZ genet var placeret på kromosomet i 54 isolater og på plasmider af forskellig størrelse i 4 isolater. Sekventering af en del af blaZ i 2 isolater viste en høj grad af homologi til de publicerede sekvenser fra humane stammer. BlaZ kunne overføres til en følsom S. aureus stamme fra de 4 isolater, hvor blaZ var placeret på plasmider, mens det ikke var muligt at overføre blaZ fra 3 isolater med kromosomal placering. BlaZ og blaR1 generne var placeret nær hinanden som tidligere beskrevet. Det var ikke muligt at observere nogen korrelation mellem fagtype og penicillinresistens, hvilket er i modsætning til det, der tidligere er blevet rapporteret for humane stammer. BlaZ er tilsyneladende stadig primært kromosomalt placeret blandt danske bovine stammer, hvilket er i modsætning til situationen blandt humane isolater, hvor der er sket et skift mod en øget forekomst af plasmidmedieret betalaktamase produktion.

References

  • Aarestrup FM, Wegener HC, Rosdahl VT: Evaluation of phenotypic and genotypic methods for epidemiological typing of Staphylococcus aureus isolates from bovine mastitis in Denmark. Vet. Microbiol. 1995, 45, 139–150.

    Article  CAS  Google Scholar 

  • Aarestrup FM, Wegener HC, Rosdahl VT, Jensen NE: Staphylococcal and other bacterial species associated with intramammary infections in Danish dairy herds. Acta Vet. Scand. 1995a, 36, 475–487.

  • Aarestrup FM, Ahrens P, Madsen M, Pallesen LV, Poulsen RL, Westh H: Glycopeptide susceptibility among Danish Enterococcus faecium and Enterococcus faecalis isolates of animal and human origin and PCR identification of genes within the VanA cluster. Antimicrob. Agents Chemother. 1996, 40, 1938–1940.

    Article  CAS  Google Scholar 

  • Aarestrup FM, Jensen NE: Development of penicillin resistance among Staphylococcus aureus isolated from bovine mastitis in Denmark and other countries. Microb. Drug Resist. 1998, 4, 247–256.

    Article  CAS  Google Scholar 

  • Asheshov EH: The genetics of penicillinase production in Staphylococcus aureus strain PS80. J. Gen. Microbiol. 1969, 59, 289–301.

    Article  CAS  Google Scholar 

  • Chan PT: Nucleotide sequence of the Staphylococcus aureus PCI beta-lactamase gene. Nucleic Acids Res. 1986, 14, 5940.

  • East AK, Dyke KGH: Cloning and sequence determination of six Staphylococcus aureus beta-lactamases and their expression in Escherichia coli and Staphylococcus aureus. J. Gen. Microbiol. 1989, 135, 1001–1015.

    CAS  Google Scholar 

  • Gillespie MT, Skurray RA: Nucleotide sequence of the blaZ gene of the Staphylococcus aureus beta-lactamase transposon Tn4002. Nucleic Acids Res. 1989, 17, 8854.

  • Kossaibati MA, Esslemont RJ: The costs of production diseases in dairy herds in England. Vet. J. 1997, 154 41–51.

  • Lacey RW: Antibiotic resistance in Staphylococcus aureus and streptococci. Brit. Med. Bull. 1984, 40, 77–83.

    Article  CAS  Google Scholar 

  • Owens WE, Watts JL: Antimicrobial susceptibility and beta-lactamase testing of staphylococci isolated from dairy herds. J. Dairy Sci. 1988, 71, 1934–1939.

    Article  CAS  Google Scholar 

  • Renneberg J, Rosdahl VT: A nationwide 30 years study of changes in antibiotic resistance in Staphylococcus aureus with special emphasis on penicillin-resistance. Zbl. Bakt. Suppl. 1992, 26, 27– 30.

  • Renneberg J, Rosdahl VT: Epidemiological studies of penicillin resistance in Danish Staphylococcus aureus strains in the period 1977–1990. Scand. J. Infect. Dis. 1992a, 24, 401–409.

  • Rosdahl VT: Localisation of the penicillinase gene in naturally occurring Staphylococcus aureus strains. Acta Path. Microbiol. Immunol. Scand. B 1985, 93, 383–388.

    CAS  Google Scholar 

  • Rosdahl VT: Epidemiology of resistance among gram-positive bacteria: With special reference to staphylococcal infections. J. Hosp. Infect. Suppl. A 1988, 11, 123–129.

    Article  Google Scholar 

  • Rowland SJ, Dyke KGH: Tn552 a novel transposable element from Staphylococcus aureus. Mol. Microbiol. 1990, 4, 961–975.

    CAS  Google Scholar 

  • Schito GC, Debbia EA, Pesce A: Susceptibility of respiratory strains of Staphylococcus aureus to fifteen antibiotics: Results of a collaborative surveillance study (1992–1993). The Alexander Project Collaborative Group. J. Antimicrob. Chemother. Suppl. A 1996, 38, 97–106.

    CAS  Google Scholar 

  • Sears LE, Moran LS, Kissinger C, Creasey T, Perry-O’Keefe H, Roskey M, Sutherland E, Slatko BE: CircumVent thermal cycle sequencing and alternative manual and automated DNA sequencing protocols using the highly thermostable VentR (exo-) DNA polymerase. Biotech. 1992, 13, 626– 633.

  • Skov RL, Williams TJ, Pallesen L, Rosdahl VT, Espersen F: Beta-lactamase production and genetic location in Staphylococcus aureus: Introduction af a beta-lactamase plasmid in strains of phage group II. J. Hosp. Infect. 1995, 30, 111–124.

    Article  CAS  Google Scholar 

  • Virarni Z, Noble WC: Antibiotic resistance and plasmids in Staphylococcus aureus from normal populations. J. Antimicrob. Chemother. 1992, 29, 35– 39.

  • Wang P-Z, Novick RP: Nucleotide sequence and expression of the beta-lactamase gene from Staphylococcus aureus plasmid pI258 in Escherichia coli, Bacillus subtilis, and Staphylococcus aureus. J. Bacteriol. 1987, 169, 1763–1766.

    Article  CAS  Google Scholar 

  • Wegener HC, Schwartz S: Antibiotic resistance and plasmids in Staphylococcus hyicus isolates from pigs with exudative epidermitis and from healthy pigs. Vet. Microbiol. 1993, 34, 363–372.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Microbiology, The Danish Veterinary Laboratory, Bülowsvej 27, DK-1790, Copenhagen V, Denmark

    M. Vesterholm-Nielsen, M. Ølholm Larsen & E. Møller Aarestrup

  2. Department of Veterinary Microbiology, The Royal Veterinary and Agricultural University, Frederiksberg, Denmark

    M. Vesterholm-Nielsen, M. Ølholm Larsen & J. Elmerdahl Olsen

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  1. M. Vesterholm-Nielsen
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  2. M. Ølholm Larsen
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  3. J. Elmerdahl Olsen
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  4. E. Møller Aarestrup
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Correspondence to E. Møller Aarestrup.

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Vesterholm-Nielsen, M., Ølholm Larsen, M., Elmerdahl Olsen, J. et al. Occurrence of the blaZ Gene in Penicillin Resistant Staphylococcus aureus Isolated from Bovine Mastitis in Denmark. Acta Vet Scand 40, 279–286 (1999). https://doi.org/10.1186/BF03547026

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Acta Veterinaria Scandinavica

ISSN: 1751-0147