Abstract: Objective To study the influence of cannabinoid CB2 receptor agonist AM1241 on cultured hepatic stellate cell line(HSC-T6).Methods HSC-T6 cells were divided into a control group(normal culture), an oxidative stress model group(treated with 100 mU/L glucose oxidase), and two AM1241 treatment groups(first treated with 20 or 80 μmol/L AM1241 and then treated with 100 mU/L glucose oxidase).Alpha-smooth muscle actin(α-SMA) expression in HSC-T6 cells was detected with immunocytochemical method.Collagen type I(Col I) content in supernatants of HSC-T6 cell culture, malondialdehyde(MDA) and superoxide dismutase(SOD) contents in HSC-T6 cells were determined with enzyme-linked immunosorbent assay(ELISA);thiobarbituric acid method for MDA and nitroblue tetrazolium method for SOD were adopted in the detections.Western blot was used to detect total protein and nuclear protein of nuclear factor erythroid-2 related factor 2(Nrf2) in HSC-T6 cells.Results Compared with the control group, the expression of Col I and α-SMA in HSC-T6 cells were significantly increased in the oxidative stress group(P<0.05) and the expression of Col I and α-SMA in HSC-T6 cells were significantly decreased (P<0.05).Compared with the control group, MDA of the oxidative stress group increased but SOD decreased significantly(both P<0.05); compared with the oxidative stress group, the content of SOD in AM1241 treatment group decreased but MDA increased significantly(both P<0.05).The expression of Nrf2 total protein in the control, oxidative stress and two AM1241 treatment groups were 0.57±0.021, 0.60±0.015, 0.59±0.020, and 0.61±0.032, respectively, without significant difference among the groups.No expression of nuclear protein of Nrf2 was detected in the control group; there was the expression of Nrf2 nuclear protein in the oxidative stress group and the expression of Nrf2 nuclear protein in the AM1241treatement increased significantly(P<0.05).Conclusion Cannabinoid CB2 receptor agonist AM1241 can inhibit HSC-T6 cell activation under oxidative stress and the effect may be related to the promoted intranuclear transfer of Nrf2 and the increased antioxidant activity in HSC-T6 cells.