Abstract: ObjectiveTo evaluate the changes of the human memory and concentration after two nights of sleep restriction.MethodsTwelve tests(6 memory tests and 6 typing tests)were conducted in two groups of medical students(n=13),druing the 2 nights of sleep restriction.Scores of the memory game and quantity of the typing test were used for the memory and concentration evaluation.Coupling t analyses on the results of last 5 tests with that of first were conducted.ResultsThere was significant difference in tests in tests'score between first test and the last 5 tests in A group(P<0.05).There was no significant difference in group B(P>0.05).ConclusionMemory game and typing test can be used to evaluate the changes of human memory and concentration after two nights of sleep restriction.