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倪朝辉, 郑文旭, 李凡. 产超广谱β-内酰胺酶大肠埃希菌耐药基因分析[J]. 中国公共卫生, 2008, 24(10): 1237-1239. DOI: 10.11847/zgggws2008-24-10-45
引用本文: 倪朝辉, 郑文旭, 李凡. 产超广谱β-内酰胺酶大肠埃希菌耐药基因分析[J]. 中国公共卫生, 2008, 24(10): 1237-1239. DOI: 10.11847/zgggws2008-24-10-45
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NI Zhao-hui, ZHENG Wen-xu, LI Fan. Analysis of drug resistance genes in extended spectrum β-lactamases producing Escherichia coli[J]. Chinese Journal of Public Health, 2008, 24(10): 1237-1239. DOI: 10.11847/zgggws2008-24-10-45
Citation: NI Zhao-hui, ZHENG Wen-xu, LI Fan. Analysis of drug resistance genes in extended spectrum β-lactamases producing Escherichia coli[J]. Chinese Journal of Public Health, 2008, 24(10): 1237-1239. DOI: 10.11847/zgggws2008-24-10-45
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产超广谱β-内酰胺酶大肠埃希菌耐药基因分析

Analysis of drug resistance genes in extended spectrum β-lactamases producing Escherichia coli

    摘要
  • 摘要: 目的 了解产超广谱β-内酰胺酶(ESBLs)大肠埃希菌分离株ESBLs基因与氨基糖苷修饰酶(AMEs)基因的流行状况.方法 对49株临床分离株采用K-B(Kirby-Bauer)纸片扩散法测定细菌的药物敏感性,应用聚合酶链反应(PCR)方法检测ESBLs基因和AMEs基因的携带状况,分析其分布特征及联合检出情况.结果 该49株产ES-BLs大肠埃希菌全部为多重耐药菌株,对氨苄西林、头孢唑啉、头孢哌酮、头孢曲松以及氨苄西林/舒巴坦的耐药率均超过90%,对庆大霉素、妥布霉素的耐药率也分别达到67.3%和65.3%,对美洛培南耐药率最低,仅为2.0%.耐药基因检测结果显示,ESBLs基因中blaTEM、blaSHV、blaCTX-M-1和blaCTX-M-9的阳性率分别为93.9%,2.2%,22.5%和59.2%.AMEs基因中aac(3)-Ⅱ、aac(6')-Ⅰ和ant(3")-Ⅰ的阳性率分别为61.2%,30.6%和18.4%.结论 临床分离的产ESBLs大肠埃希菌多重耐药现象严重,对氨基糖苷类抗生素耐药率较高.ESBLs基因与AMEs基因携带率高,且多种ESBLs基因可与AMEs基因共存,以blaTEM、blaCTX-M-9与aac(3)-Ⅱ基因共存最为多见.

     

    Abstract: Objective To investigate the genotypes prevalence of extended spectrum B-lactamases(ESBLs)and aminog lycoside modifying enzymes(AM Es)in ESBLs producing Escherichia coli clinical strains.Methods Kirby-Baucer method was performed to test the antibiotics susceptibility of 49 ESBLs pro ducing Escher ichia coli strains.Genot ypes of ESBLs and AMEs were analyzed by PCR method.Results The 49 clinical strains were all multiple resistant.The resistance rate to ampicillin,cefazolin,cefoper azone and ceftriaxone was more than 90%.The resistance rates to gentamicin and tobramycin were 67.3% and 65.3% respectively.The resistance rate to meropenem was the lowest,only 2.0%.The positive rate of ESBLs g enes of blaTEM,blaSHV,blaCTX-M-1 and blaCTX-M-9 was 9319%,2.2%,2215% and 59.2%,respectively.The positive rate of AMEs genes of aac(3)-Ⅱ,aac(6c)-Ⅰ and ant(3d)-Ⅰ was 61.2%,30.6% and 18.4%,respectively.Conclusion Multiple resistance of ESBLs producing Escher ichia coli clinical strains was serious.Different genotypes of ESBLs and AM Es were of ten coexistent in the same strain and the most popular coexist ence way was blaT EM,blaCT X-M-9 and aac(3)-Ⅱ.

     

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