大豆异黄酮对去卵巢大鼠抗氧化能力影响
Effect of soybean isoflavones on antioxidant ability in ovariectomized rats
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摘要:目的 研究大豆异黄酮对去卵巢大鼠抗氧化能力的影响。方法 3月龄SD雌性大鼠去卵巢, 术后验证造模成功, 将去势大鼠随机分为2组, 模型组给予去离子水, 干预组给予大豆异黄酮提取物灌胃。另设对照组行假手术, 给予去离子水灌胃。于第13周末处死大鼠, 氧电极法测定脑线粒体呼吸链的呼吸功能, 并测定脑线粒体和胞浆丙二醛(MDA)含量, 肝、心和肾组织匀浆谷胱甘肽过氧化物酶(GSH-Px)活性。结果 与对照组比较, 模型组脑线粒体2条呼吸链呼吸控制率(RCR)、磷氧比(P/O)和氧化磷酸化效率(OPR)有明显下降, 过氧化产物增加, 肝、心和肾组织匀浆抗氧化酶的活性也有明显下降; 用大豆异黄酮干预, 可使上述指标有明显改善。结论 大豆异黄酮能够提高去卵巢大鼠呼吸功能和肝、心、肾组织的抗氧化酶的活性, 可能与其对抗自由基氧化损伤作用有关。Abstract:Objective To investigate the influenceo of soybean isoflavones(SI)to ovariectomized rats' antioxidant ability.Methods After being ovariectomized, the 3-month old rats were divided into 2 groups randomly.They were supplememed with deionized water or SI solution by garage, The rats were sacrificed at the end of 13 weeks.The cerebral mitochondrial respiratory functions were assayed with Clark oxygen electrode.The levesl of malondialdelyde(MDA)and the activities of glutathione peroxidase(GSH2Px)were detrrmined.Results In the group of SI, rate of respiratory control(RCR), ratio of ADP and oxygen(P/O)and ratio of A TP and quantity of protein(OPR)increased significantly; the level of MDA decreased and the activity of GSH2Px increased.Conclusion The SI can enhance the ability of antioxidant in ovariectomized rats.
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