山葡萄多酚对血管内皮细胞损伤的保护作用

Protection of polyphenols of vitis amurensis rupron on injured vascular endothelial cells in vitro

摘要:
目的研究山葡萄多酚对血管内皮细胞损伤的保护作用.
方法用过氧化氢诱导血管内皮细胞损伤, 采用四甲基偶氮噻唑蓝比色法检测山葡萄多酚对细胞增殖的影响, 放射免疫法检测其对内皮素(ET-1)及前列环素(PGI₂)释放的影响.
结果过氧化氢对血管内皮细胞具有损伤作用.山葡萄多酚不同剂量抗过氧化氢引起的血管内皮细胞增殖抑制、LDH渗漏、PGI₂释放减少和ET-1释放增加.
结论山葡萄多酚具有保护血管内皮细胞作用.

Abstract:
Objective To evaluate the effects of the polyphenols of Vitis amurensis Rupron injured vascularendothelium cells.
Methods The experiment were performed in culture of H₂O₂-induced human umbilical vein endothelium cell(ECV) in vitro.Cell viability was assessed by MT Tassay.Radioimmunoassay was used to assess the released level of endothelin-1(ET-1)and prostacyclin(PGI₂).
Results H₂O₂ inhibited the ECVproliferation.Pre-incubation of ECVwith the polyphenols of Vitis amurensis Ruprbefore H₂O₂ exposure significantly increased the cell viability, the polyphenols of Vitis amurensis Ruprreduced LDH, ET-1 and increased the level of PGI₂ in H₂O₂-induced ECV.
Conclusion The po lyphenols of Vitis amurensis Ruprcan produce the protective action on injured ECV.