Abstract: Objective To study effects of fluride on apoptosis of kidney cells of rats.Methods Wistar rats were provided with distilled water containing NaF(50mg/L)for six months.Kidney cell apoptosis were detected by TUNEL(TdT-mediated dUTP Nick End Labelling)and flow cytometry.Results TUNEL-positive cells could be detected in fluoride-treated rat kidney,the apoptotic rates of fluoride-treated kidney cells were higher than those of the control significantly.Conclusions This result indicated that fluoride could induce kidney cells apoptosis of rats.