视网膜色素上皮氧化损伤SIRT1表达改变

冯俊侨; 杨柳; 周圆; 祁冰洁; 尹悦; 马乐; 张玮

doi:10.11847/zgggws1119547

视网膜色素上皮氧化损伤SIRT1表达改变

Oxidative stress-related discrepant expression of sirtuin 1 in human retinal pigment epithelial cells

摘要

摘要:
目的明确氧化应激对视网膜色素上皮细胞（RPE）中去乙酰化酶1（SIRT1）表达的影响。
方法以人RPE细胞为实验对象，不同浓度H₂O₂（0、200、300 µmol/L）处理RPE细胞，观察处理后24 h细胞形态的改变情况，检测处理后24 h与72 h细胞中SIRT1的mRNA与蛋白表达情况。
结果 H₂O₂作用后，随H₂O₂浓度的增加，RPE细胞的形态受损，有凋亡小体的出现；在氧化应激24 h后细胞内SIRT1的转录水平增加，而在氧化应激72 h后SIRT1的蛋白表达显著下降。
结论氧化应激可导致RPE细胞形态改变，SIRT1在RPE细胞内维持着氧化与抗氧化应激系统平衡，因此将SIRT1可作为临床上年龄相关性黄斑变性病（AMD）治疗的靶点。

Abstract:
Objective To explore the effect of oxidative stress on sirtuin 1 (SIRT1) expression in retinal pigment epithelial (RPE) cells.
Methods Human RPE cells were treated with hydrogen peroxide (H₂O₂) at concentrations of 0, 200, 300 µmol/L. RPE cell morphology was observed with Giemsa staining 24 hours after the treatment. SIRT1 mRNA and protein expression were detected 24 and 72 hours after H₂O₂ treatment.
Results The morphology of RPE cells was damaged and apoptotic bodies were observed after treatment with different concentrations of H₂O₂; SIRT1 mRNA level was increased in a dose-dependent manner 24 hours after H₂O₂-induced oxidative stress. However, 72 hours after H₂O₂ treatment SIRT1 protein expression was reduced significantly.
Conclusion Oxidative stress can lead to morphological changes in RPE cells; SIRT1 maintains the balance of the oxidation reduction system in RPE cells and could be used as a therapeutic target in for clinical management of age-related macular degeneration (AMD).

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