屎肠球菌对大肠杆菌感染肉鸡生长性能、血清生化指标和盲肠菌群结构的影响

doi:10.11843/j.issn.0366-6964.2018.05.011

摘要/Abstract

摘要：

旨在研究日粮中添加屎肠球菌对大肠杆菌感染的肉鸡生长性能、血清生化指标和盲肠菌群结构的影响。将360只1日龄体重接近的白羽肉鸡随机分为4组，每组6个重复（每重复15只）：阴性对照组（NC）、阳性对照组（PC）、屎肠球菌组（E.f）和抗生素组（Anti）。后3组肉鸡在7日龄感染大肠杆菌，试验共持续28 d。肉鸡的血清生化指标检测使用特定试剂盒，而盲肠微生物结构变化用PCR-DGGE检测。结果显示：1）14、21和28日龄时，屎肠球菌组肉鸡体重显著高于阴性和阳性对照组肉鸡（P<0.05）；10～14、15～21、10～28日龄屎肠球菌组肉鸡平均日增重显著高于阴性和阳性对照组肉鸡（P<0.05）；2）相较于阴性对照和阳性对照肉鸡，14和21日龄屎肠球菌组和抗生素组血清IgA浓度显著增加（P<0.05）；相较于阴性对照，10～28日龄屎肠球菌组和抗生素组血清中IgG浓度显著增加（P<0.05）；3）与21日龄阳性对照组肉鸡相比，屎肠球菌组血清中C3浓度显著增加（P<0.05）；与10、14和21日龄阴性对照肉鸡相比，其余3组肉鸡血清中溶菌酶的浓度显著增加（P<0.05）；4）与阳性对照组相比，饲喂屎肠球菌显著增加28日龄肉鸡盲肠微生物菌群的香农指数（P<0.05）。结果提示，饲喂屎肠球菌可显著提高大肠杆菌感染肉鸡的生长性能，提高血液免疫相关指数，改变盲肠菌群多样性。

Abstract:

The present study was conducted to investigate the effects of Enterococcus faecium administration on the growth performance, serum biochemical parameters and cecal microflora's structure in E. coli-challenged broilers. A total of 360 1-day-old White Feather chickens with the approximative body weight were randomly divided into 4 groups, six replicates (15 birds per replicate) in each group:negative control (NC), positive control (basal diet, PC), E. faecium-fed (E. f) and antibiotic-fed birds (Anti). The birds in PC, E. f and Anti groups were challenged with E. coli K88 on d 7, the whole test lasted for 28 d. And the serum levels of complement components and immunoglobulins were measured by the specific kits, while the change of cecal microbiota was determined by PCR-DGGE. Data showed that:1) The birds in E. f group had significant higher body weight than that of birds in NC and PC groups on d 14, 21 and 28(P<0.05); The birds in E. f group had significant higher ADG than that of birds in NC and PC groups during d 10-14, 15-21 and 10-28 (P<0.05). 2) The birds in E. f and Anti groups had significant higher level of serum IgA than that of birds in NC and PC groups on d 14, 21 (P<0.05); Birds in E. f and Anti groups had significant higher level of serum IgG than that of birds in NC group in d 10-28(P<0.05). 3) Compared with birds in PC group, birds in E. f group had significant higher C3 concentration on d 21(P<0.05). The birds in PC, E. f and Anti groups had higher serum lysozyme than that of birds in NC group on d 10, 14 and 21 (P<0.05). 4) Compared with the birds in PC group, birds in E. f group had significant higher Shannon index of cecal microflora (P<0.05) on d 28. In conclusion, the dietary Enterococcus faecium improved the growth performance, serum immune parameters and changed the diversity of cecal intestinal microflora in the E. coli K88-challenged chicken.

中图分类号:

S831.5

曹广添, 代兵, 张玲玲, 曾新福, 杨彩梅. 屎肠球菌对大肠杆菌感染肉鸡生长性能、血清生化指标和盲肠菌群结构的影响[J]. 畜牧兽医学报, 2018, 49(5): 962-970.

CAO Guang-tian, DAI Bing, ZHANG Ling-ling, ZENG Xin-fu, YANG Cai-mei. Effects of Enterococcus faecium on the Growth Performance,Serum Biochemical Parameters and the Structure of Cecal Microflora in the E. coli-challenged Broilers[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(5): 962-970.

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