SPF雏鸡感染REV后中枢免疫器官细胞凋亡及相关因子的变化

doi:10.11843/j.issn.0366-6964.2017.010.022

畜牧兽医学报 ›› 2017, Vol. 48 ›› Issue (10): 1983-1989.doi: 10.11843/j.issn.0366-6964.2017.010.022

SPF雏鸡感染REV后中枢免疫器官细胞凋亡及相关因子的变化

付礼胜¹, 侯宁^1,2, 王晓燕¹, 高雪丽¹, 刘超男¹, 吕晓萍¹, 郑世民^1*

1. 东北农业大学动物医学学院黑龙江省实验动物与比较医学重点实验室, 哈尔滨 150030;
2. 秦皇岛圣蓝皇家海洋公园, 秦皇岛 066000

收稿日期:2017-04-24 出版日期:2017-10-23 发布日期:2017-10-23
通讯作者: 郑世民,教授,E-mail:zhengshiminbl@sohu.com
作者简介:付礼胜(1991-),男,河南息县人,硕士生,主要从事动物免疫病研究工作,E-mail:m18845043769@163.com
基金资助:
国家自然科学基金（31472169）。

Changes of Cell Apoptosis and Relevant Factor in Central Immune Organs of SPF Chicks Infected with Reticuloendotheliosis Virus

FU Li-sheng¹, HOU Ning^1,2, WANG Xiao-yan¹, GAO Xue-li¹, LIU Chao-nan¹, LÜ Xiao-ping¹, ZHENG Shi-min^1*

1. Heilongjiang Key Laboratory of Laboratory Animal and Comparative Medicine, Colloge of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China;
2. Qinhuangdao Saintland Ocean Park, Qinhuangdao 066000, China

Received:2017-04-24 Online:2017-10-23 Published:2017-10-23
Supported by:

摘要/Abstract

摘要：

为探索禽网状内皮组织增生病病毒（REV）感染对雏鸡中枢免疫器官细胞凋亡及其相关酶活性和基因表达的影响，将70只1日龄SPF雏鸡随机分为REV感染组（I）和对照组（C），应用酶活性检测、荧光定量PCR、ELISA、TUNEL等方法对中枢免疫器官细胞的Caspase-3和Caspase-9活性、Bcl-2 mRNA及其蛋白质表达和凋亡细胞的动态变化进行了检测。结果发现，SPF雏鸡感染REV后，其胸腺中Caspase-3和Caspase-9活性、Bcl-2 mRNA及其蛋白质含量、凋亡细胞数量均于7~28 d内不同程度（P<0.05或P<0.01）高于对照组雏鸡；法氏囊中上述被检指标均于7~35 d内显著（P<0.05）或极显著（P<0.01）高于对照组雏鸡。研究表明REV感染引起SPF雏鸡中枢免疫器官Caspase-3和Caspase-9活性增强、Bcl-2 mRNA及其蛋白质表达上调所致的细胞凋亡与雏鸡免疫器官功能抑制密切相关。

Abstract:

The research was conducted to study the effects of reticuloendotheliosis virus (REV) on cell apoptosis, enzyme activities and expression of relevant factors in immune organs of chicks. Seventy one-day old SPF chicks were randomly selected into REV infection groups (I) and control groups (C). The methods of enzyme activities detection kit, RT-PCR, ELISA and TUNEL were applied to detect activities of Caspase-3 and Caspase-9, expression of Bcl-2 and cell apoptosis in central immune organs. The results showed that the detected indexes in thymus of SPF chicks infected with REV were significantly higher than control groups at 7 to 28 days after infection. The indexes in bursa of Fabricius of SPF chicks infected with REV were significantly increased than control groups at 7 to 35 days after infection. These data indicate that cell apoptosis caused by enhancement of Caspase-3 and Caspase-9 activities, up-regulated expression of the Bcl-2 mRNA and protein in central immune organs of SPF chicks infected with REV, are closely related to immune function suppression.

中图分类号:

S858.315.3

付礼胜, 侯宁, 王晓燕, 高雪丽, 刘超男, 吕晓萍, 郑世民. SPF雏鸡感染REV后中枢免疫器官细胞凋亡及相关因子的变化[J]. 畜牧兽医学报, 2017, 48(10): 1983-1989.

FU Li-sheng, HOU Ning, WANG Xiao-yan, GAO Xue-li, LIU Chao-nan, Lü Xiao-ping, ZHENG Shi-min. Changes of Cell Apoptosis and Relevant Factor in Central Immune Organs of SPF Chicks Infected with Reticuloendotheliosis Virus[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(10): 1983-1989.

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SPF雏鸡感染REV后中枢免疫器官细胞凋亡及相关因子的变化

Changes of Cell Apoptosis and Relevant Factor in Central Immune Organs of SPF Chicks Infected with Reticuloendotheliosis Virus

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